Crispr

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 58
• Symbol: ACTA1
• Description: actin alpha 1, skeletal muscle

DataSource: http://genomecrispr.dkfz.de/#!/results/ACTA1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
229433074	229433097	1	+	26472758	Jiyoye	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	-0.016036049218670634	[176]	[131]	0	hSpCas9	negative selection	1991
229431804	229431827	1	-	26472758	Jiyoye	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.7056151234708659	[160]	[197]	5	hSpCas9	negative selection	1992
229433040	229433063	1	-	26472758	Jiyoye	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-3.0953249590233103	[33]	[2]	-9	hSpCas9	negative selection	1993
229432682	229432705	1	-	26472758	Jiyoye	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	1.1193730705231375	[187]	[307]	7	hSpCas9	negative selection	1994
229431831	229431854	1	-	26472758	Jiyoye	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	0.40717538150587274	[0]	[0]	3	hSpCas9	negative selection	1995
229431744	229431767	1	-	26472758	Jiyoye	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	0.5190682615767706	[61]	[66]	4	hSpCas9	negative selection	1996
229432593	229432616	1	+	26472758	Jiyoye	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.8062713369156956	[90]	[119]	5	hSpCas9	negative selection	1998
229432407	229432430	1	-	26472758	Jiyoye	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.9009469138564585	[51]	[20]	-6	hSpCas9	negative selection	1999
229432009	229432032	1	+	26472758	Jiyoye	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.11243211594474145	[275]	[224]	0	hSpCas9	negative selection	2000
229431983	229432006	1	+	26472758	Jiyoye	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-1.450805613621699	[144]	[39]	-7	hSpCas9	negative selection	2001
229433074	229433097	1	+	26472758	KBM7	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.3537335183432341	[466,415]	[368,171]	4	hSpCas9	negative selection	193109
229431804	229431827	1	-	26472758	KBM7	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.4939116061184192	[791,1485]	[972,609]	5	hSpCas9	negative selection	193110
229433040	229433063	1	-	26472758	KBM7	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-2.9955783062229804	[28,6]	[0,0]	-9	hSpCas9	negative selection	193111
229432682	229432705	1	-	26472758	KBM7	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.47082935315198693	[1171,1119]	[971,548]	5	hSpCas9	negative selection	193112
229431831	229431854	1	-	26472758	KBM7	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	1.8894541142896983	[13,3]	[7,20]	9	hSpCas9	negative selection	193113
229431744	229431767	1	-	26472758	KBM7	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	0.2852459955506086	[306,258]	[141,178]	3	hSpCas9	negative selection	193114
229432593	229432616	1	+	26472758	KBM7	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	-0.015155583855530674	[615,655]	[301,294]	0	hSpCas9	negative selection	193116
229432407	229432430	1	-	26472758	KBM7	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.4832279246904414	[222,89]	[57,43]	-4	hSpCas9	negative selection	193117
229432009	229432032	1	+	26472758	KBM7	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	-0.03288453566218369	[722,592]	[322,280]	0	hSpCas9	negative selection	193118
229431983	229432006	1	+	26472758	KBM7	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.40574316642130803	[433,335]	[249,226]	4	hSpCas9	negative selection	193119
229433074	229433097	1	+	26472758	Raji	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	-0.722691463184088	[255]	[35]	-5	hSpCas9	negative selection	384227
229431804	229431827	1	-	26472758	Raji	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.12080405113592119	[641]	[161]	1	hSpCas9	negative selection	384228
229433040	229433063	1	-	26472758	Raji	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	2.1073835353735992	[0]	[0]	9	hSpCas9	negative selection	384229
229432682	229432705	1	-	26472758	Raji	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.31822173008196497	[393]	[113]	3	hSpCas9	negative selection	384230
229431831	229431854	1	-	26472758	Raji	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-1.7995070602349195	[14]	[0]	-8	hSpCas9	negative selection	384231
229431744	229431767	1	-	26472758	Raji	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.38258245141180997	[190]	[33]	-3	hSpCas9	negative selection	384232
229432593	229432616	1	+	26472758	Raji	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.15765674185482342	[281]	[72]	1	hSpCas9	negative selection	384234
229432407	229432430	1	-	26472758	Raji	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-1.1214351551222814	[74]	[7]	-7	hSpCas9	negative selection	384235
229432009	229432032	1	+	26472758	Raji	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.16639194710552263	[502]	[130]	1	hSpCas9	negative selection	384236
229431983	229432006	1	+	26472758	Raji	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.26608128139265763	[214]	[59]	2	hSpCas9	negative selection	384237
229433074	229433097	1	+	26472758	K562	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.06509502822188498	[384]	[339]	0	hSpCas9	negative selection	575345
229431804	229431827	1	-	26472758	K562	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.8158979990239812	[859]	[1277]	6	hSpCas9	negative selection	575346
229433040	229433063	1	-	26472758	K562	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.4788839813034702	[16]	[19]	4	hSpCas9	negative selection	575347
229432682	229432705	1	-	26472758	K562	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.4440198930044502	[837]	[519]	-3	hSpCas9	negative selection	575348
229431831	229431854	1	-	26472758	K562	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-3.8430441135838915	[16]	[0]	-9	hSpCas9	negative selection	575349
229431744	229431767	1	-	26472758	K562	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.06592139294570354	[247]	[199]	0	hSpCas9	negative selection	575350
229432593	229432616	1	+	26472758	K562	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.9864821894723319	[451]	[755]	8	hSpCas9	negative selection	575352
229432407	229432430	1	-	26472758	K562	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	0.0816892276283383	[74]	[66]	0	hSpCas9	negative selection	575353
229432009	229432032	1	+	26472758	K562	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.37533821981661764	[578]	[633]	3	hSpCas9	negative selection	575354
229431983	229432006	1	+	26472758	K562	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.23638140860102866	[359]	[357]	1	hSpCas9	negative selection	575355
229431534	229431557	1	-	26627737	DLD1	viability	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.34295184679076773	[1607]	[762,751,433]	3	hSpCas9	negative selection	769563
229431800	229431823	1	-	26627737	DLD1	viability	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.07416748991954097	[411]	[130,128,144]	0	hSpCas9	negative selection	769564
229432262	229432285	1	+	26627737	DLD1	viability	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.06349546017745067	[1768]	[653,603,358]	0	hSpCas9	negative selection	769565
229432563	229432586	1	-	26627737	DLD1	viability	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	2.253217075916648	[12]	[17,42,0]	9	hSpCas9	negative selection	769566
229433040	229433063	1	-	26627737	DLD1	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-1.2218710927423535	[12]	[0,0,2]	-8	hSpCas9	negative selection	769567
229433041	229433064	1	-	26627737	DLD1	viability	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	-0.42873328557178003	[121]	[32,47,7]	-4	hSpCas9	negative selection	769568
229431534	229431557	1	-	26627737	GBM cells	viability after 5 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.10108612511431447	[888]	[446,441]	-1	hSpCas9	negative selection	851878
229431800	229431823	1	-	26627737	GBM cells	viability after 5 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.21390036035420112	[249]	[142,167]	3	hSpCas9	negative selection	851879
229432262	229432285	1	+	26627737	GBM cells	viability after 5 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.31006582889247947	[570]	[237,255]	-5	hSpCas9	negative selection	851880
229432563	229432586	1	-	26627737	GBM cells	viability after 5 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	0.2681476845514048	[23]	[19,10]	4	hSpCas9	negative selection	851881
229433040	229433063	1	-	26627737	GBM cells	viability after 5 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.03641739863562021	[19]	[4,16]	0	hSpCas9	negative selection	851882
229433041	229433064	1	-	26627737	GBM cells	viability after 5 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	0.38766106034753856	[61]	[42,43]	6	hSpCas9	negative selection	851883
229431534	229431557	1	-	26627737	GBM cells	viability after 13 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.3478544577692291	[888]	[394,365]	-4	hSpCas9	negative selection	934193
229431800	229431823	1	-	26627737	GBM cells	viability after 13 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.10410754113634779	[249]	[147,143]	1	hSpCas9	negative selection	934194
229432262	229432285	1	+	26627737	GBM cells	viability after 13 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.29220830864098135	[570]	[268,239]	-3	hSpCas9	negative selection	934195
229432563	229432586	1	-	26627737	GBM cells	viability after 13 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	0.3877201104717738	[23]	[21,12]	5	hSpCas9	negative selection	934196
229433040	229433063	1	-	26627737	GBM cells	viability after 13 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.006041919746579527	[19]	[11,9]	0	hSpCas9	negative selection	934197
229433041	229433064	1	-	26627737	GBM cells	viability after 13 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	0.3600802247844457	[61]	[51,35]	4	hSpCas9	negative selection	934198
229431534	229431557	1	-	26627737	GBM cells	viability after 21 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.20742417514565492	[888]	[600,517]	2	hSpCas9	negative selection	1016508
229431800	229431823	1	-	26627737	GBM cells	viability after 21 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-0.4380126109568483	[249]	[105,94]	-4	hSpCas9	negative selection	1016509
229432262	229432285	1	+	26627737	GBM cells	viability after 21 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.14137585224441995	[570]	[286,275]	-1	hSpCas9	negative selection	1016510
229432563	229432586	1	-	26627737	GBM cells	viability after 21 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	0.8027130895882384	[23]	[16,27]	7	hSpCas9	negative selection	1016511
229433040	229433063	1	-	26627737	GBM cells	viability after 21 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.96488109840617	[19]	[3,6]	-7	hSpCas9	negative selection	1016512
229433041	229433064	1	-	26627737	GBM cells	viability after 21 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.1361400711672451	[61]	[84,63]	9	hSpCas9	negative selection	1016513
229431534	229431557	1	-	26627737	RPE1	viability after 9 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.0412982356929934	[3277]	[921,1228]	0	hSpCas9	negative selection	1098823
229431800	229431823	1	-	26627737	RPE1	viability after 9 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-0.04519649615732263	[967]	[363,202]	0	hSpCas9	negative selection	1098824
229432262	229432285	1	+	26627737	RPE1	viability after 9 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.11545726884513585	[3213]	[992,845]	-1	hSpCas9	negative selection	1098825
229432563	229432586	1	-	26627737	RPE1	viability after 9 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	-0.33352193340706693	[74]	[22,12]	-2	hSpCas9	negative selection	1098826
229433040	229433063	1	-	26627737	RPE1	viability after 9 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.640545941863357	[32]	[12,56]	8	hSpCas9	negative selection	1098827
229433041	229433064	1	-	26627737	RPE1	viability after 9 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	0.1724588257377313	[277]	[77,123]	1	hSpCas9	negative selection	1098828
229431534	229431557	1	-	26627737	RPE1	viability after 12 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.06089398807206292	[3277]	[804,1038]	0	hSpCas9	negative selection	1181138
229431800	229431823	1	-	26627737	RPE1	viability after 12 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.3329138919217327	[967]	[567,72]	2	hSpCas9	negative selection	1181139
229432262	229432285	1	+	26627737	RPE1	viability after 12 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.15130122025042558	[3213]	[1093,815]	1	hSpCas9	negative selection	1181140
229432563	229432586	1	-	26627737	RPE1	viability after 12 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	-0.6610903958474053	[74]	[20,3]	-4	hSpCas9	negative selection	1181141
229433040	229433063	1	-	26627737	RPE1	viability after 12 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.5505707595638252	[32]	[7,44]	8	hSpCas9	negative selection	1181142
229433041	229433064	1	-	26627737	RPE1	viability after 12 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	0.35265556136726284	[277]	[107,81]	2	hSpCas9	negative selection	1181143
229431534	229431557	1	-	26627737	RPE1	viability after 15 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.42779960902463965	[3277]	[726,652]	-3	hSpCas9	negative selection	1263453
229431800	229431823	1	-	26627737	RPE1	viability after 15 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-0.14352360239265738	[967]	[309,187]	-1	hSpCas9	negative selection	1263454
229432262	229432285	1	+	26627737	RPE1	viability after 15 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.10416544310758313	[3213]	[1107,592]	0	hSpCas9	negative selection	1263455
229432563	229432586	1	-	26627737	RPE1	viability after 15 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	-0.29052591150969764	[74]	[25,8]	-2	hSpCas9	negative selection	1263456
229433040	229433063	1	-	26627737	RPE1	viability after 15 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.040321158412746	[32]	[7,29]	6	hSpCas9	negative selection	1263457
229433041	229433064	1	-	26627737	RPE1	viability after 15 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	0.2269018355860506	[277]	[61,120]	1	hSpCas9	negative selection	1263458
229431534	229431557	1	-	26627737	RPE1	viability after 18 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.16324900104355833	[3277]	[774,805]	-1	hSpCas9	negative selection	1345768
229431800	229431823	1	-	26627737	RPE1	viability after 18 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.16069982743353872	[967]	[408,166]	1	hSpCas9	negative selection	1345769
229432262	229432285	1	+	26627737	RPE1	viability after 18 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.114392245662164	[3213]	[953,637]	0	hSpCas9	negative selection	1345770
229432563	229432586	1	-	26627737	RPE1	viability after 18 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	0.4065915892192775	[74]	[50,0]	2	hSpCas9	negative selection	1345771
229433040	229433063	1	-	26627737	RPE1	viability after 18 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.1181747438276273	[32]	[28,8]	6	hSpCas9	negative selection	1345772
229433041	229433064	1	-	26627737	RPE1	viability after 18 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	0.05332158140789667	[277]	[87,66]	0	hSpCas9	negative selection	1345773
229431534	229431557	1	-	26627737	HeLa	viability after 8 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.3565288056873739	[1460]	[671,563,305]	-3	hSpCas9	negative selection	1428083
229431800	229431823	1	-	26627737	HeLa	viability after 8 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	1.877745844474892	[171]	[244,260,309]	9	hSpCas9	negative selection	1428084
229432262	229432285	1	+	26627737	HeLa	viability after 8 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.9638167471732184	[547]	[527,591,300]	7	hSpCas9	negative selection	1428085
229432563	229432586	1	-	26627737	HeLa	viability after 8 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	5.820193515822526	[0]	[17,39,14]	9	hSpCas9	negative selection	1428086
229433040	229433063	1	-	26627737	HeLa	viability after 8 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	2.76163292802846	[8]	[47,3,31]	9	hSpCas9	negative selection	1428087
229433041	229433064	1	-	26627737	HeLa	viability after 8 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.8697811730970315	[52]	[120,100,41]	9	hSpCas9	negative selection	1428088
229431534	229431557	1	-	26627737	HeLa	viability after 12 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.31646115276984865	[1460]	[565,521,364]	-3	hSpCas9	negative selection	1510398
229431800	229431823	1	-	26627737	HeLa	viability after 12 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	1.182692644622078	[171]	[44,214,214]	8	hSpCas9	negative selection	1510399
229432262	229432285	1	+	26627737	HeLa	viability after 12 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.658831572037085	[547]	[474,310,294]	5	hSpCas9	negative selection	1510400
229432563	229432586	1	-	26627737	HeLa	viability after 12 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	5.9341779440140074	[0]	[21,31,20]	9	hSpCas9	negative selection	1510401
229433040	229433063	1	-	26627737	HeLa	viability after 12 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.5774334206423157	[8]	[1,1,29]	9	hSpCas9	negative selection	1510402
229433041	229433064	1	-	26627737	HeLa	viability after 12 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.9715231212721527	[52]	[58,103,91]	9	hSpCas9	negative selection	1510403
229431534	229431557	1	-	26627737	HeLa	viability after 15 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.3502190006714485	[1460]	[686,374,323]	-3	hSpCas9	negative selection	1592713
229431800	229431823	1	-	26627737	HeLa	viability after 15 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	1.8594469919738335	[171]	[157,284,325]	9	hSpCas9	negative selection	1592714
229432262	229432285	1	+	26627737	HeLa	viability after 15 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	1.2964473677107082	[547]	[649,342,657]	8	hSpCas9	negative selection	1592715
229432563	229432586	1	-	26627737	HeLa	viability after 15 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	6.155741615841262	[0]	[40,27,16]	9	hSpCas9	negative selection	1592716
229433040	229433063	1	-	26627737	HeLa	viability after 15 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	2.075866955552148	[8]	[20,0,24]	9	hSpCas9	negative selection	1592717
229433041	229433064	1	-	26627737	HeLa	viability after 15 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.4340022550217684	[52]	[47,56,70]	8	hSpCas9	negative selection	1592718
229431534	229431557	1	-	26627737	HeLa	viability after 18 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.3327794120913914	[1460]	[461,322,323]	-3	hSpCas9	negative selection	1675028
229431800	229431823	1	-	26627737	HeLa	viability after 18 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	1.8617724231485102	[171]	[105,236,325]	9	hSpCas9	negative selection	1675029
229432262	229432285	1	+	26627737	HeLa	viability after 18 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	1.28265799192572	[547]	[425,283,657]	8	hSpCas9	negative selection	1675030
229432563	229432586	1	-	26627737	HeLa	viability after 18 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	6.32760446099112	[0]	[33,23,16]	9	hSpCas9	negative selection	1675031
229433040	229433063	1	-	26627737	HeLa	viability after 18 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	2.125294372655684	[8]	[14,0,24]	9	hSpCas9	negative selection	1675032
229433041	229433064	1	-	26627737	HeLa	viability after 18 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.4966844088399454	[52]	[34,49,70]	8	hSpCas9	negative selection	1675033
229431534	229431557	1	-	26627737	HCT116	viability after 6 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.3899185078946408	[3258]	[1253,349]	2	hSpCas9	negative selection	1757343
229431800	229431823	1	-	26627737	HCT116	viability after 6 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-0.15703950945948508	[1321]	[362,88]	-1	hSpCas9	negative selection	1757344
229432262	229432285	1	+	26627737	HCT116	viability after 6 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.47855833759443756	[1505]	[510,229]	2	hSpCas9	negative selection	1757345
229432563	229432586	1	-	26627737	HCT116	viability after 6 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	2.131382892536153	[2]	[3,0]	9	hSpCas9	negative selection	1757346
229433040	229433063	1	-	26627737	HCT116	viability after 6 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-2.3633388266900512	[55]	[2,0]	-8	hSpCas9	negative selection	1757347
229433041	229433064	1	-	26627737	HCT116	viability after 6 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	3.8478536580036313	[17]	[110,1]	9	hSpCas9	negative selection	1757348
229431534	229431557	1	-	26627737	HCT116	viability after 8 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.33196179046895913	[1246]	[626,537,474]	-4	hSpCas9	negative selection	1839658
229431800	229431823	1	-	26627737	HCT116	viability after 8 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	1.8166418356817953	[98]	[223,155,196]	9	hSpCas9	negative selection	1839659
229432262	229432285	1	+	26627737	HCT116	viability after 8 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.01735827324342587	[707]	[419,287,475]	0	hSpCas9	negative selection	1839660
229432563	229432586	1	-	26627737	HCT116	viability after 8 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	5.0041446913365775	[0]	[12,24,14]	9	hSpCas9	negative selection	1839661
229433040	229433063	1	-	26627737	HCT116	viability after 8 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.20102976787110327	[30]	[22,18,16]	2	hSpCas9	negative selection	1839662
229433041	229433064	1	-	26627737	HCT116	viability after 8 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	-0.48402294340512664	[132]	[34,78,42]	-5	hSpCas9	negative selection	1839663
229431534	229431557	1	-	26627737	HCT116	viability after 9 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.24163922974963636	[3258]	[1215,880]	1	hSpCas9	negative selection	1921973
229431800	229431823	1	-	26627737	HCT116	viability after 9 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-0.29937980107331763	[1321]	[211,345]	-2	hSpCas9	negative selection	1921974
229432262	229432285	1	+	26627737	HCT116	viability after 9 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.6026611915124476	[1505]	[780,475]	4	hSpCas9	negative selection	1921975
229432563	229432586	1	-	26627737	HCT116	viability after 9 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	3.7411444440278627	[2]	[22,0]	9	hSpCas9	negative selection	1921976
229433040	229433063	1	-	26627737	HCT116	viability after 9 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.2548742725120028	[55]	[16,8]	-2	hSpCas9	negative selection	1921977
229433041	229433064	1	-	26627737	HCT116	viability after 9 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	3.0910309123788977	[17]	[69,17]	9	hSpCas9	negative selection	1921978
229431534	229431557	1	-	26627737	HCT116	viability after 12 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.5123947445695696	[1246]	[622,256,478]	-5	hSpCas9	negative selection	2004288
229431800	229431823	1	-	26627737	HCT116	viability after 12 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	1.5732113464919137	[98]	[145,186,132]	9	hSpCas9	negative selection	2004289
229432262	229432285	1	+	26627737	HCT116	viability after 12 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	-0.11363550638398578	[707]	[330,351,349]	-1	hSpCas9	negative selection	2004290
229432563	229432586	1	-	26627737	HCT116	viability after 12 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	4.743745947912528	[0]	[18,21,0]	9	hSpCas9	negative selection	2004291
229433040	229433063	1	-	26627737	HCT116	viability after 12 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.28928065358552324	[30]	[13,15,9]	-3	hSpCas9	negative selection	2004292
229433041	229433064	1	-	26627737	HCT116	viability after 12 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	-0.6293693951584123	[132]	[24,72,39]	-6	hSpCas9	negative selection	2004293
229431534	229431557	1	-	26627737	HCT116	viability after 15 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.10984659984422684	[1246]	[722,847,429]	-1	hSpCas9	negative selection	2086603
229431800	229431823	1	-	26627737	HCT116	viability after 15 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	2.165292642298736	[98]	[238,257,265]	9	hSpCas9	negative selection	2086604
229432262	229432285	1	+	26627737	HCT116	viability after 15 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.3230587879876061	[707]	[455,509,552]	3	hSpCas9	negative selection	2086605
229432563	229432586	1	-	26627737	HCT116	viability after 15 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	5.551754786372218	[0]	[3,44,28]	9	hSpCas9	negative selection	2086606
229433040	229433063	1	-	26627737	HCT116	viability after 15 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.2845186133999289	[30]	[29,8,26]	3	hSpCas9	negative selection	2086607
229433041	229433064	1	-	26627737	HCT116	viability after 15 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	-1.2642237617026193	[132]	[51,25,19]	-8	hSpCas9	negative selection	2086608
229431534	229431557	1	-	26627737	HCT116	viability after 18 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.00987694151835683	[1246]	[631,673,505]	0	hSpCas9	negative selection	2168918
229431800	229431823	1	-	26627737	HCT116	viability after 18 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	2.036430170648797	[98]	[251,207,124]	9	hSpCas9	negative selection	2168919
229432262	229432285	1	+	26627737	HCT116	viability after 18 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.19539420011698216	[707]	[363,255,572]	1	hSpCas9	negative selection	2168920
229432563	229432586	1	-	26627737	HCT116	viability after 18 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	5.168319755485228	[0]	[0,33,15]	9	hSpCas9	negative selection	2168921
229433040	229433063	1	-	26627737	HCT116	viability after 18 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.689007132867518	[30]	[14,5,6]	-6	hSpCas9	negative selection	2168922
229433041	229433064	1	-	26627737	HCT116	viability after 18 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	-1.5333444779928869	[132]	[24,17,23]	-8	hSpCas9	negative selection	2168923
229432506	229432529	1	+	25494202	A375	resistance to PLX-4720 (puromycin)	GGGGGCGGGGGCGGGGGCGGGAG	ACTA1	ENSG00000143632	2.532533376556697	[0,1]	[0,2]	8	dCas9-VP64	positive selection	2465329
229432500	229432523	1	+	25494202	A375	resistance to PLX-4720 (puromycin)	GGGGGCGGGGGCGGGGGCGGGGG	ACTA1	ENSG00000143632	2.532533376556697	[0,1]	[0,2]	8	dCas9-VP64	positive selection	2465344
229432506	229432529	1	+	25494202	A375	resistance to PLX-4720 (zeocin)	GGGGGCGGGGGCGGGGGCGGGAG	ACTA1	ENSG00000143632	0.4348111379075954	[3,1]	[0,0]	3	dCas9-VP64	positive selection	2560584
229432500	229432523	1	+	25494202	A375	resistance to PLX-4720 (zeocin)	GGGGGCGGGGGCGGGGGCGGGGG	ACTA1	ENSG00000143632	0.4348111379075954	[3,1]	[0,0]	3	dCas9-VP64	positive selection	2560599
229432682	229432705	1	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.14305732676180205	[3,2]	[4,0]	-2	hSpCas9	positive selection	2968670
229433069	229433092	1	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GGAGCCATTGTCGCACACGAGGG	ACTA1	ENSG00000143632	0.6977498140143162	[2,2]	[1,5]	9	hSpCas9	positive selection	2968671
229432840	229432863	1	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GGTCAGAAAGATTCCTACGTGGG	ACTA1	ENSG00000143632	0.33343865289393704	[3,2]	[2,4]	7	hSpCas9	positive selection	2968672
229433040	229433063	1	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.3822738425595638	[2,3]	[2,0]	-5	hSpCas9	positive selection	2968673
229432682	229432705	1	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.06743439785514571	[3,3]	[3,1]	1	hSpCas9	positive selection	3036528
229433069	229433092	1	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GGAGCCATTGTCGCACACGAGGG	ACTA1	ENSG00000143632	0.3065667323954889	[2,2]	[2,2]	5	hSpCas9	positive selection	3036529
229432840	229432863	1	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GGTCAGAAAGATTCCTACGTGGG	ACTA1	ENSG00000143632	0.9014241786592796	[3,3]	[4,4]	9	hSpCas9	positive selection	3036530
229433040	229433063	1	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.5695598556912427	[2,2]	[0,0]	-7	hSpCas9	positive selection	3036531
229432601	229432624		-	24336571	A375	resistance to PLX after 7 days	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.10268871934303592	[19,18]	[19,21]	3	hSpCas9	positive selection	3159553
229432367	229432390		+	24336571	A375	resistance to PLX after 7 days	CAGCGCGTAGCCCTCATAAATGG	ACTA1	ENSG00000143632	0.007858553284908587	[5,6]	[6,4]	0	hSpCas9	positive selection	3159564
229432420	229432443		-	24336571	A375	resistance to PLX after 7 days	CGCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.351227685943722	[14,12]	[16,18]	8	hSpCas9	positive selection	3159575
229432548	229432571		+	24336571	A375	resistance to PLX after 7 days	GCACTCACCGGTGGTCCTGCCGG	ACTA1	ENSG00000143632	-0.09871810404738596	[9,8]	[9,6]	-3	hSpCas9	positive selection	3159584
229432605	229432628		+	24336571	A375	resistance to PLX after 7 days	GTACATGGCGGGCACGTTGAAGG	ACTA1	ENSG00000143632	-0.0024252853761282944	[7,7]	[6,7]	0	hSpCas9	positive selection	3159589
229432394	229432417		+	24336571	A375	resistance to PLX after 7 days	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.26622308462892375	[7,8]	[6,6]	-7	hSpCas9	positive selection	3159595
229432601	229432624		-	24336571	A375	resistance to PLX after 14 days	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.7221377348476756	[24,19]	[6,5]	-8	hSpCas9	positive selection	3217346
229432367	229432390		+	24336571	A375	resistance to PLX after 14 days	CAGCGCGTAGCCCTCATAAATGG	ACTA1	ENSG00000143632	-0.32269633579369006	[4,9]	[1,2]	-4	hSpCas9	positive selection	3217357
229432420	229432443		-	24336571	A375	resistance to PLX after 14 days	CGCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	1.4913286652266684	[15,15]	[18,21]	9	hSpCas9	positive selection	3217368
229432548	229432571		+	24336571	A375	resistance to PLX after 14 days	GCACTCACCGGTGGTCCTGCCGG	ACTA1	ENSG00000143632	0.2977967202229424	[9,5]	[3,5]	3	hSpCas9	positive selection	3217377
229432605	229432628		+	24336571	A375	resistance to PLX after 14 days	GTACATGGCGGGCACGTTGAAGG	ACTA1	ENSG00000143632	-0.5589882436451141	[11,4]	[1,2]	-6	hSpCas9	positive selection	3217382
229432394	229432417		+	24336571	A375	resistance to PLX after 14 days	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.6095897358118649	[10,5]	[1,1]	-7	hSpCas9	positive selection	3217388
229432601	229432624		-	24336571	A375	viability after 7 days	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	1.1616498359104872	[8]	[19,18]	9	hSpCas9	negative selection	3275139
229432367	229432390		+	24336571	A375	viability after 7 days	CAGCGCGTAGCCCTCATAAATGG	ACTA1	ENSG00000143632	0.05181537017129778	[5]	[5,6]	0	hSpCas9	negative selection	3275150
229432420	229432443		-	24336571	A375	viability after 7 days	CGCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.4012151845178066	[10]	[14,12]	6	hSpCas9	negative selection	3275161
229432548	229432571		+	24336571	A375	viability after 7 days	GCACTCACCGGTGGTCCTGCCGG	ACTA1	ENSG00000143632	0.19809558658843968	[8]	[9,8]	3	hSpCas9	negative selection	3275170
229432605	229432628		+	24336571	A375	viability after 7 days	GTACATGGCGGGCACGTTGAAGG	ACTA1	ENSG00000143632	0.7255751308013071	[4]	[7,7]	9	hSpCas9	negative selection	3275175
229432394	229432417		+	24336571	A375	viability after 7 days	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.12836961432764993	[7]	[7,8]	2	hSpCas9	negative selection	3275181
229432601	229432624		-	24336571	A375	viability after 14 days	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	1.379120271969107	[8]	[24,19]	9	hSpCas9	negative selection	3332932
229432367	229432390		+	24336571	A375	viability after 14 days	CAGCGCGTAGCCCTCATAAATGG	ACTA1	ENSG00000143632	0.2542378269682144	[5]	[4,9]	3	hSpCas9	negative selection	3332943
229432420	229432443		-	24336571	A375	viability after 14 days	CGCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.5577353630470512	[10]	[15,15]	7	hSpCas9	negative selection	3332954
229432548	229432571		+	24336571	A375	viability after 14 days	GCACTCACCGGTGGTCCTGCCGG	ACTA1	ENSG00000143632	0.04632418890468426	[8]	[9,5]	0	hSpCas9	negative selection	3332963
229432605	229432628		+	24336571	A375	viability after 14 days	GTACATGGCGGGCACGTTGAAGG	ACTA1	ENSG00000143632	0.9272431646270252	[4]	[11,4]	9	hSpCas9	negative selection	3332968
229432394	229432417		+	24336571	A375	viability after 14 days	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.14318405672663492	[7]	[10,5]	1	hSpCas9	negative selection	3332974
229431799	229431822	1	+	27383988	293T	resistance to West Nile virus (flavivirus)	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.023048820956533694	[160,139]	[0,0]	0	hSpCas9	positive selection	3335382
229431781	229431804	1	-	27383988	293T	resistance to West Nile virus (flavivirus)	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	5.089394709064512	[163,154]	[0,69]	9	hSpCas9	positive selection	3335383
229432321	229432344	1	+	27383988	293T	resistance to West Nile virus (flavivirus)	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.26027590587123056	[87,167]	[0,0]	1	hSpCas9	positive selection	3335384
229432394	229432417	1	+	27383988	293T	resistance to West Nile virus (flavivirus)	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	2.7978945928085377	[21,21]	[0,0]	8	hSpCas9	positive selection	3354632
229432601	229432624	1	-	27383988	293T	resistance to West Nile virus (flavivirus)	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.12804319450086854	[279,279]	[1,1]	0	hSpCas9	positive selection	3354633
229432021	229432044	1	-	27383988	293T	resistance to West Nile virus (flavivirus)	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	1.107579091941153	[70,70]	[0,0]	5	hSpCas9	positive selection	3354634
229432682	229432705	1	-	26780180	HT29	viability (Avana library 4 designs)	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.824088445450115	[]	[]	3	hSpCas9	negative selection	3404618
229433068	229433091	1	+	26780180	HT29	viability (Avana library 4 designs)	GGAGCCATTGTCGCACACGAGGG	ACTA1	ENSG00000143632	1.49893569907362	[]	[]	6	hSpCas9	negative selection	3404619
229432840	229432863	1	-	26780180	HT29	viability (Avana library 4 designs)	GGTCAGAAAGATTCCTACGTGGG	ACTA1	ENSG00000143632	2.39724897800881	[]	[]	8	hSpCas9	negative selection	3404620
229433040	229433063	1	-	26780180	HT29	viability (Avana library 4 designs)	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.53077891688225	[]	[]	6	hSpCas9	negative selection	3404621
229433040	229433063	1	-	26780180	A375	viability (Avana lentiCRISPRv2)	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-4.48449369517582	[]	[]	-5	hSpCas9	negative selection	3476243
229432682	229432705	1	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	2.89564278897364	[]	[]	4	hSpCas9	negative selection	3476244
229433068	229433091	1	+	26780180	A375	viability (Avana lentiCRISPRv2)	GGAGCCATTGTCGCACACGAGGG	ACTA1	ENSG00000143632	-10.5629029249557	[]	[]	-8	hSpCas9	negative selection	3476245
229432840	229432863	1	-	26780180	A375	viability (Avana lentiCRISPRv2)	GGTCAGAAAGATTCCTACGTGGG	ACTA1	ENSG00000143632	-1.68142190728887	[]	[]	-2	hSpCas9	negative selection	3476246
229432715	229432738	1	-	26780180	A375	viability (Avana lentiCRISPRv2)	CTTCTACAACGAGCTTCGCGTGG	ACTA1	ENSG00000143632	-2.91112793189499	[]	[]	-3	hSpCas9	negative selection	3476247
229433071	229433094	1	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCCATTGTCGCACACGAGGGCGG	ACTA1	ENSG00000143632	3.47245633948674	[]	[]	5	hSpCas9	negative selection	3476248
229433040	229433063	1	-	26780180	A375	viability (Avana lentiGuide)	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	12.0719250113908	[]	[]	7	hSpCas9	negative selection	3584902
229432682	229432705	1	-	26780180	A375	viability (Avana lentiGuide)	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	2.53273391893576	[]	[]	2	hSpCas9	negative selection	3584903
229433068	229433091	1	+	26780180	A375	viability (Avana lentiGuide)	GGAGCCATTGTCGCACACGAGGG	ACTA1	ENSG00000143632	1.19855449220084	[]	[]	1	hSpCas9	negative selection	3584904
229432840	229432863	1	-	26780180	A375	viability (Avana lentiGuide)	GGTCAGAAAGATTCCTACGTGGG	ACTA1	ENSG00000143632	13.7680264838573	[]	[]	7	hSpCas9	negative selection	3584905
229432715	229432738	1	-	26780180	A375	viability (Avana lentiGuide)	CTTCTACAACGAGCTTCGCGTGG	ACTA1	ENSG00000143632	2.49544753654083	[]	[]	2	hSpCas9	negative selection	3584906
229433071	229433094	1	+	26780180	A375	viability (Avana lentiGuide)	GCCATTGTCGCACACGAGGGCGG	ACTA1	ENSG00000143632	13.908922502385	[]	[]	7	hSpCas9	negative selection	3584907
229433040	229433063	1	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.3387619322590223	[4]	[3,3]	-5	hSpCas9	positive selection	3693561
229432682	229432705	1	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.3541238111822855	[3]	[3,1]	-5	hSpCas9	positive selection	3693562
229433068	229433091	1	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GGAGCCATTGTCGCACACGAGGG	ACTA1	ENSG00000143632	-0.783548637041876	[3]	[2,1]	-8	hSpCas9	positive selection	3693563
229432840	229432863	1	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GGTCAGAAAGATTCCTACGTGGG	ACTA1	ENSG00000143632	-0.4910974019151963	[4]	[3,1]	-6	hSpCas9	positive selection	3693564
229432715	229432738	1	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CTTCTACAACGAGCTTCGCGTGG	ACTA1	ENSG00000143632	0.05556814755728673	[4]	[4,3]	1	hSpCas9	positive selection	3693565
229433071	229433094	1	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCCATTGTCGCACACGAGGGCGG	ACTA1	ENSG00000143632	0.08109151937525085	[4]	[3,4]	2	hSpCas9	positive selection	3693566
229433040	229433063	1	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-1.1013687881360603	[4]	[0,1]	-8	hSpCas9	positive selection	3802168
229432682	229432705	1	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.578845289664303	[4]	[2,1]	-6	hSpCas9	positive selection	3802169
229433068	229433091	1	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GGAGCCATTGTCGCACACGAGGG	ACTA1	ENSG00000143632	-0.606946943947696	[4]	[0,3]	-6	hSpCas9	positive selection	3802170
229432840	229432863	1	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GGTCAGAAAGATTCCTACGTGGG	ACTA1	ENSG00000143632	-1.0711517541603206	[4]	[2,0]	-8	hSpCas9	positive selection	3802171
229432715	229432738	1	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CTTCTACAACGAGCTTCGCGTGG	ACTA1	ENSG00000143632	-0.3610037505518282	[4]	[2,3]	-5	hSpCas9	positive selection	3802172
229433071	229433094	1	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCCATTGTCGCACACGAGGGCGG	ACTA1	ENSG00000143632	-0.1407694922573306	[5]	[3,3]	-2	hSpCas9	positive selection	3802173
229432021	229432044	1	-	26780180	A375	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	1.2592711654221	[]	[]	6	hSpCas9	negative selection	3910779
229432601	229432624	1	-	26780180	A375	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	1.03474358689568	[]	[]	5	hSpCas9	negative selection	3910780
229432321	229432344	1	+	26780180	A375	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.628387725522588	[]	[]	2	hSpCas9	negative selection	3910781
229431799	229431822	1	+	26780180	A375	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	1.52886864933669	[]	[]	8	hSpCas9	negative selection	3910782
229431781	229431804	1	-	26780180	A375	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	0.892305331926814	[]	[]	4	hSpCas9	negative selection	3910783
229432394	229432417	1	+	26780180	A375	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.871922483597639	[]	[]	3	hSpCas9	negative selection	3910784
229432021	229432044	1	-	26780180	HT29	viability (GeCKOv2 library)	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	1.46760587948988	[]	[]	7	hSpCas9	negative selection	4018745
229432601	229432624	1	-	26780180	HT29	viability (GeCKOv2 library)	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.948555697991661	[]	[]	4	hSpCas9	negative selection	4018746
229432321	229432344	1	+	26780180	HT29	viability (GeCKOv2 library)	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.7037920480875	[]	[]	2	hSpCas9	negative selection	4018747
229431799	229431822	1	+	26780180	HT29	viability (GeCKOv2 library)	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.734056438736634	[]	[]	3	hSpCas9	negative selection	4018748
229431781	229431804	1	-	26780180	HT29	viability (GeCKOv2 library)	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	1.60009158241366	[]	[]	8	hSpCas9	negative selection	4018749
229432394	229432417	1	+	26780180	HT29	viability (GeCKOv2 library)	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	1.21931440978034	[]	[]	6	hSpCas9	negative selection	4018750
229432601	229432624	1	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.12148791018390467	[4]	[2,2]	-3	hSpCas9	positive selection	4187415
229432367	229432390	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CAGCGCGTAGCCCTCATAAATGG	ACTA1	ENSG00000143632	-0.5745972568800882	[3]	[0,0]	-9	hSpCas9	positive selection	4187426
229432420	229432443	1	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CGCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.21973079614638946	[3]	[1,4]	5	hSpCas9	positive selection	4187437
229432548	229432571	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GCACTCACCGGTGGTCCTGCCGG	ACTA1	ENSG00000143632	-0.31057663031541693	[3]	[0,1]	-6	hSpCas9	positive selection	4187447
229432605	229432628	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GTACATGGCGGGCACGTTGAAGG	ACTA1	ENSG00000143632	-0.3501951555479983	[2]	[1,0]	-7	hSpCas9	positive selection	4187458
229432394	229432417	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.2021592386706177	[2]	[0,0]	-5	hSpCas9	positive selection	4187469
229432601	229432624	1	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.16874016982492923	[3]	[1,2,4,0]	1	hSpCas9	positive selection	4251437
229432367	229432390	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CAGCGCGTAGCCCTCATAAATGG	ACTA1	ENSG00000143632	0.2276970799794292	[2]	[3,2,0,0]	1	hSpCas9	positive selection	4251448
229432420	229432443	1	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CGCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	-0.106779676680545	[2]	[0,2,1,0]	-1	hSpCas9	positive selection	4251459
229432548	229432571	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GCACTCACCGGTGGTCCTGCCGG	ACTA1	ENSG00000143632	-0.19773904918739538	[2]	[0,1,0,0]	-3	hSpCas9	positive selection	4251469
229432605	229432628	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GTACATGGCGGGCACGTTGAAGG	ACTA1	ENSG00000143632	0.17000217952472696	[1]	[0,2,0,0]	1	hSpCas9	positive selection	4251480
229432394	229432417	1	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.4846071192958987	[1]	[3,1,0,0]	3	hSpCas9	positive selection	4251491
229431799	229431822	1	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.4155583679911699	[3]	[4,3]	8	hSpCas9	positive selection	4254299
229431781	229431804	1	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	0.07585792926898771	[3]	[3,2]	1	hSpCas9	positive selection	4254300
229432321	229432344	1	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.17531725325579647	[3]	[2,2]	3	hSpCas9	positive selection	4254301
229432394	229432417	1	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.5246066420294587	[1]	[0,0]	-8	hSpCas9	positive selection	4318582
229432601	229432624	1	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.014316157858509454	[3]	[3,1]	0	hSpCas9	positive selection	4318583
229432021	229432044	1	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.8344395294730265	[2]	[0,0]	-9	hSpCas9	positive selection	4318584
229432313	229432335	1	+	27760321	OCIAML3	viability	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	-0.5956951369795896	[444,421]	[188,270]	-5	hSpCas9	negative selection	4375837
229432420	229432442	1	-	27760321	OCIAML3	viability	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.13683017360883942	[691,613]	[445,709]	1	hSpCas9	negative selection	4375838
229432552	229432574	1	+	27760321	OCIAML3	viability	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.3297366716181144	[588,574]	[476,698]	4	hSpCas9	negative selection	4375839
229433010	229433032	1	+	27760321	OCIAML3	viability	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.9210527532266639	[585,522]	[773,879]	9	hSpCas9	negative selection	4375840
229433026	229433048	1	-	27760321	OCIAML3	viability	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	-0.16265880362227725	[1034,800]	[427,908]	-2	hSpCas9	negative selection	4375841
229432313	229432335	1	+	27760321	OCIAML2	viability	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	0.40330653653872994	[444,421]	[377,577]	5	hSpCas9	negative selection	4459300
229432420	229432442	1	-	27760321	OCIAML2	viability	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.06938368675376094	[691,613]	[476,653]	0	hSpCas9	negative selection	4459301
229432552	229432574	1	+	27760321	OCIAML2	viability	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	-0.32459260203532586	[588,574]	[381,369]	-4	hSpCas9	negative selection	4459302
229433010	229433032	1	+	27760321	OCIAML2	viability	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	-0.00495642476299335	[585,522]	[385,525]	0	hSpCas9	negative selection	4459303
229433026	229433048	1	-	27760321	OCIAML2	viability	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	-0.24258195746302247	[1034,800]	[491,795]	-3	hSpCas9	negative selection	4459304
229432313	229432335	1	+	27760321	MV411	viability	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	-0.06235896721795536	[444,421]	[115,511]	0	hSpCas9	negative selection	4542763
229432420	229432442	1	-	27760321	MV411	viability	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.6225081018401688	[691,613]	[591,767]	4	hSpCas9	negative selection	4542764
229432552	229432574	1	+	27760321	MV411	viability	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.2833349679148611	[588,574]	[322,686]	2	hSpCas9	negative selection	4542765
229433010	229433032	1	+	27760321	MV411	viability	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	1.8755553886469511	[585,522]	[1879,530]	9	hSpCas9	negative selection	4542766
229433026	229433048	1	-	27760321	MV411	viability	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	1.1583893484385988	[1034,800]	[1660,863]	8	hSpCas9	negative selection	4542767
229432313	229432335	1	+	27760321	HL60	viability	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	0.04976378455521942	[444,421]	[298,438]	0	hSpCas9	negative selection	4626226
229432420	229432442	1	-	27760321	HL60	viability	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.02348299392401576	[691,613]	[633,442]	0	hSpCas9	negative selection	4626227
229432552	229432574	1	+	27760321	HL60	viability	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	-0.1220168314156112	[588,574]	[416,459]	-1	hSpCas9	negative selection	4626228
229433010	229433032	1	+	27760321	HL60	viability	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.7449611929230946	[585,522]	[710,807]	8	hSpCas9	negative selection	4626229
229433026	229433048	1	-	27760321	HL60	viability	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	-0.03424598407064772	[1034,800]	[595,865]	0	hSpCas9	negative selection	4626230
229432313	229432335	1	+	27760321	HT1080	viability	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	-0.543621570998804	[421,329]	[97,107]	-2	hSpCas9	negative selection	4709689
229432420	229432442	1	-	27760321	HT1080	viability	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	-0.5849273651504686	[613,478]	[126,164]	-3	hSpCas9	negative selection	4709690
229432552	229432574	1	+	27760321	HT1080	viability	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	-2.7117228457938127	[574,448]	[33,27]	-7	hSpCas9	negative selection	4709691
229433010	229433032	1	+	27760321	HT1080	viability	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	1.8599511773295718	[522,407]	[875,450]	8	hSpCas9	negative selection	4709692
229433026	229433048	1	-	27760321	HT1080	viability	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.25602237305125364	[800,624]	[437,230]	1	hSpCas9	negative selection	4709693
229432313	229432335	1	+	27760321	HT29	viability after 7 days	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	-0.2926559374450653	[421,329]	[220,421,511]	-4	hSpCas9	negative selection	4793152
229432420	229432442	1	-	27760321	HT29	viability after 7 days	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.6203691980917251	[613,478]	[1080,984,1169]	8	hSpCas9	negative selection	4793153
229432552	229432574	1	+	27760321	HT29	viability after 7 days	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.28912565104744903	[574,448]	[808,905,700]	4	hSpCas9	negative selection	4793154
229433010	229433032	1	+	27760321	HT29	viability after 7 days	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.19036271148196016	[522,407]	[583,969,485]	3	hSpCas9	negative selection	4793155
229433026	229433048	1	-	27760321	HT29	viability after 7 days	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.4614297619580794	[800,624]	[1641,713,1475]	7	hSpCas9	negative selection	4793156
229432313	229432335	1	+	27760321	HT29	viability after 25 days	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	0.19412169343548405	[421,329]	[516,563,379]	2	hSpCas9	negative selection	4876615
229432420	229432442	1	-	27760321	HT29	viability after 25 days	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.602903925929056	[613,478]	[620,1008,1267]	6	hSpCas9	negative selection	4876616
229432552	229432574	1	+	27760321	HT29	viability after 25 days	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.5751450506660304	[574,448]	[788,735,1119]	6	hSpCas9	negative selection	4876617
229433010	229433032	1	+	27760321	HT29	viability after 25 days	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.6175696373255206	[522,407]	[717,742,1012]	7	hSpCas9	negative selection	4876618
229433026	229433048	1	-	27760321	HT29	viability after 25 days	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.5696220954292761	[800,624]	[1109,1134,1407]	6	hSpCas9	negative selection	4876619
229432313	229432335	1	+	27760321	HT29	viability after 22 days	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	0.3869387314234567	[421,329]	[602,575,524]	4	hSpCas9	negative selection	4960078
229432420	229432442	1	-	27760321	HT29	viability after 22 days	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.6806616403950706	[613,478]	[889,844,1308]	7	hSpCas9	negative selection	4960079
229432552	229432574	1	+	27760321	HT29	viability after 22 days	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.579370269427406	[574,448]	[1097,822,724]	6	hSpCas9	negative selection	4960080
229433010	229433032	1	+	27760321	HT29	viability after 22 days	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.6507382103194965	[522,407]	[805,1045,681]	7	hSpCas9	negative selection	4960081
229433026	229433048	1	-	27760321	HT29	viability after 22 days	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.6691281020413731	[800,624]	[1846,1010,1056]	7	hSpCas9	negative selection	4960082
229432313	229432335	1	+	27760321	HT29	viability after 19 days	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	0.7126337621266159	[421,329]	[721,856,542]	7	hSpCas9	negative selection	5043541
229432420	229432442	1	-	27760321	HT29	viability after 19 days	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.5675999083996962	[613,478]	[953,789,1032]	6	hSpCas9	negative selection	5043542
229432552	229432574	1	+	27760321	HT29	viability after 19 days	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.34707380400036536	[574,448]	[1077,662,535]	3	hSpCas9	negative selection	5043543
229433010	229433032	1	+	27760321	HT29	viability after 19 days	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.44641614893026127	[522,407]	[885,832,482]	4	hSpCas9	negative selection	5043544
229433026	229433048	1	-	27760321	HT29	viability after 19 days	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.7063599980531952	[800,624]	[1410,1040,1538]	7	hSpCas9	negative selection	5043545
229432313	229432335	1	+	27760321	HT29	viability after 16 days	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	-0.14498701779393067	[421,329]	[474,393,280]	-1	hSpCas9	negative selection	5127004
229432420	229432442	1	-	27760321	HT29	viability after 16 days	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.7210612673590905	[613,478]	[832,1078,1092]	8	hSpCas9	negative selection	5127005
229432552	229432574	1	+	27760321	HT29	viability after 16 days	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.6179784712033195	[574,448]	[1106,873,677]	7	hSpCas9	negative selection	5127006
229433010	229433032	1	+	27760321	HT29	viability after 16 days	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.785518377018851	[522,407]	[896,968,824]	8	hSpCas9	negative selection	5127007
229433026	229433048	1	-	27760321	HT29	viability after 16 days	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.6584672371460618	[800,624]	[1394,1101,1296]	7	hSpCas9	negative selection	5127008
229432313	229432335	1	+	27760321	HT29	viability after 13 days	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	-0.17007769623959476	[421,329]	[464,361,338]	-2	hSpCas9	negative selection	5210467
229432420	229432442	1	-	27760321	HT29	viability after 13 days	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	0.23883306225465106	[613,478]	[635,817,787]	3	hSpCas9	negative selection	5210468
229432552	229432574	1	+	27760321	HT29	viability after 13 days	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.6330772230812717	[574,448]	[1135,791,839]	7	hSpCas9	negative selection	5210469
229433010	229433032	1	+	27760321	HT29	viability after 13 days	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.6243046324736748	[522,407]	[761,1085,659]	7	hSpCas9	negative selection	5210470
229433026	229433048	1	-	27760321	HT29	viability after 13 days	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.40079430909940417	[800,624]	[1167,986,1118]	5	hSpCas9	negative selection	5210471
229432313	229432335	1	+	27760321	HT29	viability after 10 days	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	-0.28892878691391144	[421,329]	[516,267,364]	-4	hSpCas9	negative selection	5293930
229432420	229432442	1	-	27760321	HT29	viability after 10 days	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	-0.06780452787104607	[613,478]	[527,848,582]	-1	hSpCas9	negative selection	5293931
229432552	229432574	1	+	27760321	HT29	viability after 10 days	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.48170480494794643	[574,448]	[1038,848,790]	6	hSpCas9	negative selection	5293932
229433010	229433032	1	+	27760321	HT29	viability after 10 days	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.4531389343102511	[522,407]	[932,897,563]	6	hSpCas9	negative selection	5293933
229433026	229433048	1	-	27760321	HT29	viability after 10 days	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.5245664112857285	[800,624]	[1326,1149,1358]	7	hSpCas9	negative selection	5293934
229432313	229432335	1	+	27760321	MOLM13	viability	CAGGTAGTCGGTGAGATCGCGG	ACTA1	ENSG00000143632	0.4794251834211961	[444,421]	[322,356]	3	hSpCas9	negative selection	5377393
229432420	229432442	1	-	27760321	MOLM13	viability	GCGCGCACAGGCATCGTGCTGG	ACTA1	ENSG00000143632	1.7802628949001706	[691,613]	[2104,546]	9	hSpCas9	negative selection	5377394
229432552	229432574	1	+	27760321	MOLM13	viability	TCACCGGTGGTCCTGCCGGAGG	ACTA1	ENSG00000143632	0.31704461035271714	[588,574]	[530,306]	2	hSpCas9	negative selection	5377395
229433010	229433032	1	+	27760321	MOLM13	viability	TGGACGGGAACACGGCCCTAGG	ACTA1	ENSG00000143632	0.25245672802066577	[585,522]	[443,310]	1	hSpCas9	negative selection	5377396
229433026	229433048	1	-	27760321	MOLM13	viability	CGCCGGGGATGACGCCCCTAGG	ACTA1	ENSG00000143632	0.21193038105358641	[1034,800]	[728,481]	1	hSpCas9	negative selection	5377397
229432548	229432571	1	+	27661255	K562	viability	GCACTCACCGGTGGTCCTGCCGG	ACTA1	ENSG00000143632	-0.012856785717812458	[629,664]	[631,409]	0	dCas9-KRAB	negative selection	5460687
229432560	229432583	1	+	27661255	K562	viability	GGTCCTGCCGGAGGCGTAGAGGG	ACTA1	ENSG00000143632	-0.02651748056649396	[696,762]	[617,531]	-1	dCas9-KRAB	negative selection	5460688
229432974	229432997	1	+	27661255	K562	viability	GGGGCAGCCTGACCTGGTGTCGG	ACTA1	ENSG00000143632	0.08876339847472342	[899,1102]	[946,762]	3	dCas9-KRAB	negative selection	5460689
229432977	229433000	1	+	27661255	K562	viability	GCAGCCTGACCTGGTGTCGGGGG	ACTA1	ENSG00000143632	0.10883161562857642	[720,879]	[773,612]	4	dCas9-KRAB	negative selection	5460690
229433011	229433034	1	+	27661255	K562	viability	GGACGGGAACACGGCCCTAGGGG	ACTA1	ENSG00000143632	-0.11325829769834167	[1280,1041]	[861,859]	-4	dCas9-KRAB	negative selection	5460691
229434012	229434035	1	+	27661255	K562	viability	GTAGCTACAACTGCTACTCTCGG	ACTA1	ENSG00000143632	0.1188550545081406	[248,246]	[246,187]	4	dCas9-KRAB	negative selection	5460692
229434033	229434056	1	+	27661255	K562	viability	GGCTCGGGCCCTGGTCGCCGCGG	ACTA1	ENSG00000143632	-0.05450015438833089	[936,980]	[891,604]	-2	dCas9-KRAB	negative selection	5460693
229433936	229433959	1	-	27661255	K562	viability	GGGGAACCTGAAGGACTCCGGGG	ACTA1	ENSG00000143632	0.16366061210837196	[591,681]	[581,556]	6	dCas9-KRAB	negative selection	5460694
229433955	229433978	1	-	27661255	K562	viability	GGGGACAGGGCACTACCGAGGGG	ACTA1	ENSG00000143632	0.05282642025146733	[969,1092]	[908,804]	1	dCas9-KRAB	negative selection	5460695
229434050	229434073	1	-	27661255	K562	viability	GAAGCCTCGCTTCCCCTCCGCGG	ACTA1	ENSG00000143632	-5.405255410992294	[730,849]	[9,18]	-9	dCas9-KRAB	negative selection	5460696
229431799	229431822	1	+	27260156	BXPC3	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.10021278675492196	[733]	[767,588,828,670]	-2	hSpCas9	negative selection	5526759
229431781	229431804	1	-	27260156	BXPC3	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.3071442399991299	[883]	[576,701,812,936]	-6	hSpCas9	negative selection	5526760
229432321	229432344	1	+	27260156	BXPC3	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.27668881641951726	[474]	[573,583,648,599]	6	hSpCas9	negative selection	5526761
229432394	229432417	1	+	27260156	BXPC3	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.15619892522890044	[96]	[112,77,108,158]	3	hSpCas9	negative selection	5591042
229432601	229432624	1	-	27260156	BXPC3	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.046335935460812716	[726]	[649,758,773,768]	-1	hSpCas9	negative selection	5591043
229432021	229432044	1	-	27260156	BXPC3	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.008472909156217234	[216]	[281,223,205,175]	0	hSpCas9	negative selection	5591044
229431799	229431822	1	+	27260156	A673	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.013575326797198528	[733]	[854,889,926,879]	0	hSpCas9	negative selection	5648010
229431781	229431804	1	-	27260156	A673	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.38262535307245427	[883]	[952,691,745,887]	-7	hSpCas9	negative selection	5648011
229432321	229432344	1	+	27260156	A673	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.3872766037445606	[474]	[803,656,711,822]	8	hSpCas9	negative selection	5648012
229432394	229432417	1	+	27260156	A673	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.1288344915713613	[96]	[137,108,71,110]	-3	hSpCas9	negative selection	5712293
229432601	229432624	1	-	27260156	A673	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.021070797688939935	[726]	[922,839,805,983]	0	hSpCas9	negative selection	5712294
229432021	229432044	1	-	27260156	A673	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.1660760816204585	[216]	[257,221,201,250]	-4	hSpCas9	negative selection	5712295
229431799	229431822	1	+	27260156	A375	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.18022404412531456	[733]	[690,1691,903,722]	2	hSpCas9	negative selection	5769261
229431781	229431804	1	-	27260156	A375	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.09457644047725111	[883]	[918,1089,1034,850]	-1	hSpCas9	negative selection	5769262
229432321	229432344	1	+	27260156	A375	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.5138478032581986	[474]	[785,879,758,756]	7	hSpCas9	negative selection	5769263
229432394	229432417	1	+	27260156	A375	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.7861893619461249	[96]	[97,16,139,7]	-7	hSpCas9	negative selection	5833544
229432601	229432624	1	-	27260156	A375	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.05981229745270267	[726]	[1068,1114,933,498]	0	hSpCas9	negative selection	5833545
229432021	229432044	1	-	27260156	A375	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.31336824054971546	[216]	[261,315,417,267]	4	hSpCas9	negative selection	5833546
229431799	229431822	1	+	27260156	COLO741	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.12450969555086044	[733]	[814,924,836]	-3	hSpCas9	negative selection	5890512
229431781	229431804	1	-	27260156	COLO741	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.06938620684616759	[883]	[1293,944,1010]	-1	hSpCas9	negative selection	5890513
229432321	229432344	1	+	27260156	COLO741	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.6575610166499289	[474]	[1019,826,1022]	9	hSpCas9	negative selection	5890514
229432394	229432417	1	+	27260156	COLO741	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.9187362515773518	[96]	[50,115,30]	-9	hSpCas9	negative selection	5954795
229432601	229432624	1	-	27260156	COLO741	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.17021649402478356	[726]	[788,805,872]	-4	hSpCas9	negative selection	5954796
229432021	229432044	1	-	27260156	COLO741	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.3282100512724301	[216]	[419,330,301]	6	hSpCas9	negative selection	5954797
229431799	229431822	1	+	27260156	CAL120	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.2957227884213187	[733]	[1125,1575,669]	4	hSpCas9	negative selection	6011763
229431781	229431804	1	-	27260156	CAL120	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.03382784610825462	[883]	[1390,1039,843]	0	hSpCas9	negative selection	6011764
229432321	229432344	1	+	27260156	CAL120	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.48030233182909987	[474]	[1011,742,747]	7	hSpCas9	negative selection	6011765
229432394	229432417	1	+	27260156	CAL120	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.18020228018846973	[96]	[89,60,164]	-3	hSpCas9	negative selection	6076046
229432601	229432624	1	-	27260156	CAL120	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.3554138757303581	[726]	[850,824,1738]	5	hSpCas9	negative selection	6076047
229432021	229432044	1	-	27260156	CAL120	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.04494043402794334	[216]	[288,224,274]	0	hSpCas9	negative selection	6076048
229431799	229431822	1	+	27260156	CADOES1	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.14869183375937606	[733]	[738,786,533,394]	-3	hSpCas9	negative selection	6133014
229431781	229431804	1	-	27260156	CADOES1	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.4674081671099383	[883]	[606,808,496,460]	-7	hSpCas9	negative selection	6133015
229432321	229432344	1	+	27260156	CADOES1	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.5886899064301502	[474]	[627,740,516,775]	8	hSpCas9	negative selection	6133016
229432394	229432417	1	+	27260156	CADOES1	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.5384950326617073	[96]	[46,58,117,22]	-7	hSpCas9	negative selection	6197297
229432601	229432624	1	-	27260156	CADOES1	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.055213099672244415	[726]	[626,554,639,785]	-1	hSpCas9	negative selection	6197298
229432021	229432044	1	-	27260156	CADOES1	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.3340683562409945	[216]	[84,172,161,222]	-5	hSpCas9	negative selection	6197299
229431799	229431822	1	+	27260156	EWS502	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.167751847470044	[733]	[786,709,957,1066]	3	hSpCas9	negative selection	6254265
229431781	229431804	1	-	27260156	EWS502	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.283564146437565	[883]	[709,705,892,776]	-5	hSpCas9	negative selection	6254266
229432321	229432344	1	+	27260156	EWS502	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.45953831917667287	[474]	[593,741,782,653]	7	hSpCas9	negative selection	6254267
229432394	229432417	1	+	27260156	EWS502	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.2484437536410491	[96]	[104,144,80,159]	4	hSpCas9	negative selection	6318548
229432601	229432624	1	-	27260156	EWS502	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.19666048302917127	[726]	[880,1092,673,865]	3	hSpCas9	negative selection	6318549
229432021	229432044	1	-	27260156	EWS502	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.34729145880082546	[216]	[351,315,245,241]	6	hSpCas9	negative selection	6318550
229431799	229431822	1	+	27260156	EW8	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.08264591487237483	[733]	[973,501,555,535]	1	hSpCas9	negative selection	6375516
229431781	229431804	1	-	27260156	EW8	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.38613269844131415	[883]	[805,450,514,452]	-6	hSpCas9	negative selection	6375517
229432321	229432344	1	+	27260156	EW8	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.5056550760288308	[474]	[579,406,529,672]	8	hSpCas9	negative selection	6375518
229432394	229432417	1	+	27260156	EW8	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.4330057591670706	[96]	[21,98,133,154]	7	hSpCas9	negative selection	6439799
229432601	229432624	1	-	27260156	EW8	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.07743641523135314	[726]	[797,706,509,508]	1	hSpCas9	negative selection	6439800
229432021	229432044	1	-	27260156	EW8	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.4639507086909438	[216]	[362,188,126,322]	8	hSpCas9	negative selection	6439801
229431799	229431822	1	+	27260156	CORL105	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.0408506123148501	[733]	[793,807,969,974]	0	hSpCas9	negative selection	6496767
229431781	229431804	1	-	27260156	CORL105	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.17128538772506818	[883]	[856,747,1224,894]	-3	hSpCas9	negative selection	6496768
229432321	229432344	1	+	27260156	CORL105	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.6064487393139848	[474]	[765,768,975,893]	9	hSpCas9	negative selection	6496769
229432394	229432417	1	+	27260156	CORL105	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.9082227915594823	[96]	[90,53,39,54]	-9	hSpCas9	negative selection	6561050
229432601	229432624	1	-	27260156	CORL105	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.16433140159610132	[726]	[768,863,619,760]	-3	hSpCas9	negative selection	6561051
229432021	229432044	1	-	27260156	CORL105	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.22923312238865234	[216]	[287,365,243,282]	4	hSpCas9	negative selection	6561052
229431799	229431822	1	+	27260156	HS294T	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.27278521244623366	[733]	[276,321,412,369]	3	hSpCas9	negative selection	6618018
229431781	229431804	1	-	27260156	HS294T	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.49355039058076555	[883]	[235,236,265,229]	-6	hSpCas9	negative selection	6618019
229432321	229432344	1	+	27260156	HS294T	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.1914930660062628	[474]	[171,289,182,190]	2	hSpCas9	negative selection	6618020
229432394	229432417	1	+	27260156	HS294T	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.6440295212093057	[96]	[14,22,23,36]	-7	hSpCas9	negative selection	6682301
229432601	229432624	1	-	27260156	HS294T	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.2034913324713623	[726]	[290,362,234,422]	2	hSpCas9	negative selection	6682302
229432021	229432044	1	-	27260156	HS294T	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.001948571601828819	[216]	[72,132,55,70]	0	hSpCas9	negative selection	6682303
229431799	229431822	1	+	27260156	HCC44	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.3046390359550959	[733]	[1120,629,1303,1146]	5	hSpCas9	negative selection	6739269
229431781	229431804	1	-	27260156	HCC44	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.030833393819615806	[883]	[1066,919,1185,791]	0	hSpCas9	negative selection	6739270
229432321	229432344	1	+	27260156	HCC44	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.3348347632869977	[474]	[640,535,1076,540]	5	hSpCas9	negative selection	6739271
229432394	229432417	1	+	27260156	HCC44	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.8059417197861451	[96]	[137,13,58,52]	-8	hSpCas9	negative selection	6803552
229432601	229432624	1	-	27260156	HCC44	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.06866412893857332	[726]	[718,616,1061,796]	-1	hSpCas9	negative selection	6803553
229432021	229432044	1	-	27260156	HCC44	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.2188839566933911	[216]	[286,257,298,301]	3	hSpCas9	negative selection	6803554
229431799	229431822	1	+	27260156	G402	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.2655134269739189	[733]	[1414,858,1253,822]	3	hSpCas9	negative selection	6860520
229431781	229431804	1	-	27260156	G402	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.18257728459288436	[883]	[1070,1226,804,738]	-2	hSpCas9	negative selection	6860521
229432321	229432344	1	+	27260156	G402	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.7174543736154143	[474]	[1452,691,970,758]	8	hSpCas9	negative selection	6860522
229432394	229432417	1	+	27260156	G402	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.8847532926104762	[96]	[88,38,59,69]	-8	hSpCas9	negative selection	6924803
229432601	229432624	1	-	27260156	G402	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.027959802292167768	[726]	[956,848,809,997]	0	hSpCas9	negative selection	6924804
229432021	229432044	1	-	27260156	G402	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.7547162452518752	[216]	[452,253,895,184]	8	hSpCas9	negative selection	6924805
229431799	229431822	1	+	27260156	L33	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.06303394592895983	[733]	[725,353,403,577]	-1	hSpCas9	negative selection	6981771
229431781	229431804	1	-	27260156	L33	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.2637720202096966	[883]	[703,343,438,703]	-6	hSpCas9	negative selection	6981772
229432321	229432344	1	+	27260156	L33	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	-0.11607549843953936	[474]	[408,203,342,302]	-3	hSpCas9	negative selection	6981773
229432394	229432417	1	+	27260156	L33	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.4308956985376682	[96]	[65,32,49,61]	-8	hSpCas9	negative selection	7046054
229432601	229432624	1	-	27260156	L33	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.06958979196194126	[726]	[724,300,503,790]	1	hSpCas9	negative selection	7046055
229432021	229432044	1	-	27260156	L33	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.22716567014854627	[216]	[147,68,129,218]	-5	hSpCas9	negative selection	7046056
229431799	229431822	1	+	27260156	K562	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.11755807446320221	[733]	[643,759,782,641]	1	hSpCas9	negative selection	7103022
229431781	229431804	1	-	27260156	K562	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.5378236229899358	[883]	[572,718,487,406]	-6	hSpCas9	negative selection	7103023
229432321	229432344	1	+	27260156	K562	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.750369533159142	[474]	[621,793,962,478]	8	hSpCas9	negative selection	7103024
229432394	229432417	1	+	27260156	K562	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.9452466720951063	[96]	[131,29,15,10]	-8	hSpCas9	negative selection	7167305
229432601	229432624	1	-	27260156	K562	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.27281782552090406	[726]	[481,544,1187,853]	3	hSpCas9	negative selection	7167306
229432021	229432044	1	-	27260156	K562	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.5236180789015809	[216]	[215,493,308,112]	6	hSpCas9	negative selection	7167307
229431799	229431822	1	+	27260156	HT29	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.1626935912580299	[733]	[879,760,748,795]	-3	hSpCas9	negative selection	7224273
229431781	229431804	1	-	27260156	HT29	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.27648442104142557	[883]	[893,1196,829,674]	-5	hSpCas9	negative selection	7224274
229432321	229432344	1	+	27260156	HT29	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.39797200387431875	[474]	[895,900,715,570]	7	hSpCas9	negative selection	7224275
229432394	229432417	1	+	27260156	HT29	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.11310181457970714	[96]	[135,63,101,128]	-2	hSpCas9	negative selection	7288556
229432601	229432624	1	-	27260156	HT29	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.08488826987895204	[726]	[859,912,820,751]	-2	hSpCas9	negative selection	7288557
229432021	229432044	1	-	27260156	HT29	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.08504922949176197	[216]	[309,263,280,264]	1	hSpCas9	negative selection	7288558
229431799	229431822	1	+	27260156	MHHES1	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.10886744881114685	[733]	[930,891,867,462]	-2	hSpCas9	negative selection	7345524
229431781	229431804	1	-	27260156	MHHES1	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.3470046375506177	[883]	[725,1039,699,687]	-6	hSpCas9	negative selection	7345525
229432321	229432344	1	+	27260156	MHHES1	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.4254047615893185	[474]	[948,834,601,549]	7	hSpCas9	negative selection	7345526
229432394	229432417	1	+	27260156	MHHES1	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.249844270727543	[96]	[47,115,128,73]	-5	hSpCas9	negative selection	7409807
229432601	229432624	1	-	27260156	MHHES1	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.20924347972975782	[726]	[818,816,664,571]	-4	hSpCas9	negative selection	7409808
229432021	229432044	1	-	27260156	MHHES1	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.3597875707923641	[216]	[308,327,241,357]	6	hSpCas9	negative selection	7409809
229431799	229431822	1	+	27260156	MEWO	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.20989705684601156	[733]	[1016,734,673]	4	hSpCas9	negative selection	7466775
229431781	229431804	1	-	27260156	MEWO	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	0.021127035473130884	[883]	[913,840,776]	0	hSpCas9	negative selection	7466776
229432321	229432344	1	+	27260156	MEWO	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.1416795190827678	[474]	[558,480,443]	3	hSpCas9	negative selection	7466777
229432394	229432417	1	+	27260156	MEWO	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.027092170702938567	[96]	[54,118,95]	0	hSpCas9	negative selection	7531058
229432601	229432624	1	-	27260156	MEWO	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.05702196458278985	[726]	[794,570,622]	-1	hSpCas9	negative selection	7531059
229432021	229432044	1	-	27260156	MEWO	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.25556193511348013	[216]	[174,189,145]	-5	hSpCas9	negative selection	7531060
229431799	229431822	1	+	27260156	LNCAPCLONEFGC	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.15999435322729838	[733]	[818,668,638,831]	2	hSpCas9	negative selection	7588026
229431781	229431804	1	-	27260156	LNCAPCLONEFGC	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.0558360409158406	[883]	[582,696,1029,817]	-1	hSpCas9	negative selection	7588027
229432321	229432344	1	+	27260156	LNCAPCLONEFGC	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.7174120373550271	[474]	[553,734,790,762]	9	hSpCas9	negative selection	7588028
229432394	229432417	1	+	27260156	LNCAPCLONEFGC	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.4829156240048107	[96]	[49,36,140,36]	-6	hSpCas9	negative selection	7652309
229432601	229432624	1	-	27260156	LNCAPCLONEFGC	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.2739400694188141	[726]	[651,634,1011,930]	4	hSpCas9	negative selection	7652310
229432021	229432044	1	-	27260156	LNCAPCLONEFGC	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.37893106589429026	[216]	[207,213,302,307]	6	hSpCas9	negative selection	7652311
229431799	229431822	1	+	27260156	PANC0327	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.21977688538601914	[733]	[582,779,847,808]	3	hSpCas9	negative selection	7709277
229431781	229431804	1	-	27260156	PANC0327	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.4363713796868645	[883]	[894,429,442,597]	-6	hSpCas9	negative selection	7709278
229432321	229432344	1	+	27260156	PANC0327	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.6516078982163624	[474]	[527,941,575,557]	8	hSpCas9	negative selection	7709279
229432394	229432417	1	+	27260156	PANC0327	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.5387617275042298	[96]	[67,256,115,37]	7	hSpCas9	negative selection	7773560
229432601	229432624	1	-	27260156	PANC0327	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.03262970618434047	[726]	[823,578,634,627]	0	hSpCas9	negative selection	7773561
229432021	229432044	1	-	27260156	PANC0327	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.22836342368316098	[216]	[185,132,290,301]	3	hSpCas9	negative selection	7773562
229431799	229431822	1	+	27260156	NCIH2009	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.07577678914042907	[733]	[1173,817,967]	-1	hSpCas9	negative selection	7830528
229431781	229431804	1	-	27260156	NCIH2009	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.30482073692698775	[883]	[940,1122,919]	-4	hSpCas9	negative selection	7830529
229432321	229432344	1	+	27260156	NCIH2009	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.6392732288330083	[474]	[1519,612,1069]	8	hSpCas9	negative selection	7830530
229432394	229432417	1	+	27260156	NCIH2009	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.5205690541933206	[96]	[98,102,81]	-6	hSpCas9	negative selection	7894811
229432601	229432624	1	-	27260156	NCIH2009	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.5453217262046437	[726]	[1788,1207,1512]	7	hSpCas9	negative selection	7894812
229432021	229432044	1	-	27260156	NCIH2009	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.08761332734725213	[216]	[305,205,348]	-1	hSpCas9	negative selection	7894813
229431799	229431822	1	+	27260156	NCIH1373	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.3253777950656245	[733]	[1094,964,941,299]	3	hSpCas9	negative selection	7951779
229431781	229431804	1	-	27260156	NCIH1373	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	0.03713966637502886	[883]	[1051,776,829,384]	0	hSpCas9	negative selection	7951780
229432321	229432344	1	+	27260156	NCIH1373	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.5290538705286864	[474]	[863,743,703,203]	5	hSpCas9	negative selection	7951781
229432394	229432417	1	+	27260156	NCIH1373	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.2547825139698121	[96]	[84,80,67,34]	-3	hSpCas9	negative selection	8016062
229432601	229432624	1	-	27260156	NCIH1373	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.3215221191576858	[726]	[1525,765,970,237]	3	hSpCas9	negative selection	8016063
229432021	229432044	1	-	27260156	NCIH1373	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.7536643690515359	[216]	[558,324,561,54]	7	hSpCas9	negative selection	8016064
229431799	229431822	1	+	27260156	PATU8902	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.004145744481110825	[733]	[903,1595,709,959]	0	hSpCas9	negative selection	8073030
229431781	229431804	1	-	27260156	PATU8902	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.7115076638504315	[883]	[887,530,561,1109]	-6	hSpCas9	negative selection	8073031
229432321	229432344	1	+	27260156	PATU8902	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	1.0476597280233322	[474]	[1216,1568,948,1898]	9	hSpCas9	negative selection	8073032
229432394	229432417	1	+	27260156	PATU8902	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.2758351544930906	[96]	[122,259,20,58]	-3	hSpCas9	negative selection	8137313
229432601	229432624	1	-	27260156	PATU8902	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.5799657559596674	[726]	[1148,2022,1092,1943]	6	hSpCas9	negative selection	8137314
229432021	229432044	1	-	27260156	PATU8902	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.5444078528268698	[216]	[194,135,348,1195]	5	hSpCas9	negative selection	8137315
229431799	229431822	1	+	27260156	PANC1	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.07778529041530291	[733]	[600,616,712,473]	-1	hSpCas9	negative selection	8194281
229431781	229431804	1	-	27260156	PANC1	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.07323135936711922	[883]	[686,902,780,567]	-1	hSpCas9	negative selection	8194282
229432321	229432344	1	+	27260156	PANC1	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.3909021781469114	[474]	[623,578,697,280]	6	hSpCas9	negative selection	8194283
229432394	229432417	1	+	27260156	PANC1	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.26683052734158785	[96]	[99,86,45,48]	-4	hSpCas9	negative selection	8258564
229432601	229432624	1	-	27260156	PANC1	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.1541659931683521	[726]	[530,543,625,535]	-3	hSpCas9	negative selection	8258565
229432021	229432044	1	-	27260156	PANC1	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.5584472259370963	[216]	[85,136,174,111]	-7	hSpCas9	negative selection	8258566
229431799	229431822	1	+	27260156	PANC0813	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.09793068622196532	[733]	[919,775,878,664]	-2	hSpCas9	negative selection	8315532
229431781	229431804	1	-	27260156	PANC0813	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.39867504452121005	[883]	[948,636,783,793]	-6	hSpCas9	negative selection	8315533
229432321	229432344	1	+	27260156	PANC0813	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.3496878953593815	[474]	[879,734,470,767]	5	hSpCas9	negative selection	8315534
229432394	229432417	1	+	27260156	PANC0813	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.0910865193335959	[96]	[174,129,98,87]	1	hSpCas9	negative selection	8379815
229432601	229432624	1	-	27260156	PANC0813	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.1348409024899172	[726]	[1046,901,1081,739]	2	hSpCas9	negative selection	8379816
229432021	229432044	1	-	27260156	PANC0813	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.20842538497052376	[216]	[352,289,283,257]	3	hSpCas9	negative selection	8379817
229431799	229431822	1	+	27260156	RDES	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.16848852860714703	[733]	[621,716,906,775]	-3	hSpCas9	negative selection	8436783
229431781	229431804	1	-	27260156	RDES	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.4563807340573701	[883]	[585,723,710,992]	-7	hSpCas9	negative selection	8436784
229432321	229432344	1	+	27260156	RDES	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.6044708748745751	[474]	[589,777,1138,865]	8	hSpCas9	negative selection	8436785
229432394	229432417	1	+	27260156	RDES	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.6277213730037015	[96]	[76,51,69,90]	-8	hSpCas9	negative selection	8501066
229432601	229432624	1	-	27260156	RDES	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.13507501487793167	[726]	[624,704,739,1026]	-2	hSpCas9	negative selection	8501067
229432021	229432044	1	-	27260156	RDES	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.15490137971027895	[216]	[208,274,252,398]	2	hSpCas9	negative selection	8501068
229431799	229431822	1	+	27260156	PC3	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.16607542404706332	[733]	[419,695,1582,1006]	4	hSpCas9	negative selection	8558034
229431781	229431804	1	-	27260156	PC3	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.15521197957716182	[883]	[504,662,1734,680]	-3	hSpCas9	negative selection	8558035
229432321	229432344	1	+	27260156	PC3	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.16096586150602438	[474]	[290,499,957,598]	3	hSpCas9	negative selection	8558036
229432394	229432417	1	+	27260156	PC3	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.13999878161599133	[96]	[99,103,183,53]	3	hSpCas9	negative selection	8622317
229432601	229432624	1	-	27260156	PC3	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.145826704085802	[726]	[649,781,1117,745]	3	hSpCas9	negative selection	8622318
229432021	229432044	1	-	27260156	PC3	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.020406242784857254	[216]	[108,216,378,241]	0	hSpCas9	negative selection	8622319
229431799	229431822	1	+	27260156	PATU8988T	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.11360550325362406	[733]	[403,871,578,799]	-2	hSpCas9	negative selection	8679285
229431781	229431804	1	-	27260156	PATU8988T	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	0.008225764882820008	[883]	[1028,1169,613,637]	0	hSpCas9	negative selection	8679286
229432321	229432344	1	+	27260156	PATU8988T	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.20129555858549858	[474]	[361,537,540,682]	3	hSpCas9	negative selection	8679287
229432394	229432417	1	+	27260156	PATU8988T	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.005158933069478344	[96]	[139,46,79,105]	0	hSpCas9	negative selection	8743568
229432601	229432624	1	-	27260156	PATU8988T	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.05425197211936961	[726]	[761,744,757,646]	0	hSpCas9	negative selection	8743569
229432021	229432044	1	-	27260156	PATU8988T	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.0572779090096156	[216]	[72,228,207,306]	-1	hSpCas9	negative selection	8743570
229431799	229431822	1	+	27260156	T47D	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.0034743222713702338	[733]	[864,772,819,964]	0	hSpCas9	negative selection	8800536
229431781	229431804	1	-	27260156	T47D	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.31708328208336434	[883]	[902,724,818,869]	-6	hSpCas9	negative selection	8800537
229432321	229432344	1	+	27260156	T47D	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.21960015509448066	[474]	[580,563,742,695]	5	hSpCas9	negative selection	8800538
229432394	229432417	1	+	27260156	T47D	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.3010314582619844	[96]	[112,87,105,58]	-6	hSpCas9	negative selection	8864819
229432601	229432624	1	-	27260156	T47D	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.005919108407790752	[726]	[818,769,951,836]	0	hSpCas9	negative selection	8864820
229432021	229432044	1	-	27260156	T47D	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.08079832175646451	[216]	[272,232,214,235]	-2	hSpCas9	negative selection	8864821
229431799	229431822	1	+	27260156	SU8686	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.4099362742221049	[733]	[953,777,898,1308]	7	hSpCas9	negative selection	8921787
229431781	229431804	1	-	27260156	SU8686	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.18610845736162895	[883]	[639,775,792,902]	-3	hSpCas9	negative selection	8921788
229432321	229432344	1	+	27260156	SU8686	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.38125834854829327	[474]	[535,546,518,909]	6	hSpCas9	negative selection	8921789
229432394	229432417	1	+	27260156	SU8686	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.33857216064591267	[96]	[72,51,81,104]	-5	hSpCas9	negative selection	8986070
229432601	229432624	1	-	27260156	SU8686	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	0.07457736914149216	[726]	[744,606,777,953]	1	hSpCas9	negative selection	8986071
229432021	229432044	1	-	27260156	SU8686	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.13364463552820727	[216]	[131,151,309,206]	-2	hSpCas9	negative selection	8986072
229431799	229431822	1	+	27260156	SKES1	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.14563311200396406	[733]	[670,867,684,465]	-3	hSpCas9	negative selection	9043038
229431781	229431804	1	-	27260156	SKES1	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.4157479045058207	[883]	[662,725,636,623]	-7	hSpCas9	negative selection	9043039
229432321	229432344	1	+	27260156	SKES1	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.5319021129691642	[474]	[751,788,656,570]	9	hSpCas9	negative selection	9043040
229432394	229432417	1	+	27260156	SKES1	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.2196074183950305	[96]	[67,84,108,68]	-5	hSpCas9	negative selection	9107321
229432601	229432624	1	-	27260156	SKES1	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.1669125015125414	[726]	[806,751,500,563]	-4	hSpCas9	negative selection	9107322
229432021	229432044	1	-	27260156	SKES1	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.0006867888816173284	[216]	[206,209,217,224]	0	hSpCas9	negative selection	9107323
229431799	229431822	1	+	27260156	TOV112D	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.15026745472761066	[733]	[764,745,801,372]	-3	hSpCas9	negative selection	9164289
229431781	229431804	1	-	27260156	TOV112D	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.11745606937208408	[883]	[696,1110,782,736]	-2	hSpCas9	negative selection	9164290
229432321	229432344	1	+	27260156	TOV112D	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.20294684059690096	[474]	[613,778,455,406]	4	hSpCas9	negative selection	9164291
229432394	229432417	1	+	27260156	TOV112D	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	0.3853862694093375	[96]	[59,396,84,28]	7	hSpCas9	negative selection	9228572
229432601	229432624	1	-	27260156	TOV112D	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.08317060772621532	[726]	[746,665,756,580]	-2	hSpCas9	negative selection	9228573
229432021	229432044	1	-	27260156	TOV112D	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	0.1559725456880533	[216]	[308,355,208,130]	3	hSpCas9	negative selection	9228574
229431799	229431822	1	+	27260156	TC71	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	0.09304326393930051	[733]	[823,775,881,875]	2	hSpCas9	negative selection	9285540
229431781	229431804	1	-	27260156	TC71	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.4013288562358899	[883]	[664,793,682,730]	-8	hSpCas9	negative selection	9285541
229432321	229432344	1	+	27260156	TC71	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.2047920202560725	[474]	[544,659,568,573]	5	hSpCas9	negative selection	9285542
229432394	229432417	1	+	27260156	TC71	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.1954396580698603	[96]	[125,71,53,112]	-5	hSpCas9	negative selection	9349823
229432601	229432624	1	-	27260156	TC71	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.07558645580622192	[726]	[623,801,741,795]	-2	hSpCas9	negative selection	9349824
229432021	229432044	1	-	27260156	TC71	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.32836324691096763	[216]	[133,172,248,183]	-7	hSpCas9	negative selection	9349825
229431799	229431822	1	+	27260156	TC32	viability	GCCCCCCGACATGACGTTGTTGG	ACTA1	ENSG00000143632	-0.18717869464134995	[733]	[625,487,729,604]	-3	hSpCas9	negative selection	9406791
229431781	229431804	1	-	27260156	TC32	viability	GGGGCACCACGATGTACCCTGGG	ACTA1	ENSG00000143632	-0.6957007921827386	[883]	[365,459,691,575]	-8	hSpCas9	negative selection	9406792
229432321	229432344	1	+	27260156	TC32	viability	CGGTGAGATCGCGGCCCGCCAGG	ACTA1	ENSG00000143632	0.5839409234270087	[474]	[590,454,720,988]	8	hSpCas9	negative selection	9406793
229432394	229432417	1	+	27260156	TC32	viability	GTTGTGGGTGACGCCGTCGCCGG	ACTA1	ENSG00000143632	-0.3226826157818645	[96]	[37,63,56,136]	-5	hSpCas9	negative selection	9471074
229432601	229432624	1	-	27260156	TC32	viability	CAACGTGCCCGCCATGTACGTGG	ACTA1	ENSG00000143632	-0.25743358522169035	[726]	[474,375,722,805]	-5	hSpCas9	negative selection	9471075
229432021	229432044	1	-	27260156	TC32	viability	CAACGAGCGCTTCCGCTGCCCGG	ACTA1	ENSG00000143632	-0.17944773032184558	[216]	[184,120,241,194]	-3	hSpCas9	negative selection	9471076
229431534	229431557	1	-	27869803	HPAFII	viability after 27 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.16982608704521507	[808]	[550,233,NaN]	-1	hSpCas9	negative selection	9532551
229431800	229431823	1	-	27869803	HPAFII	viability after 27 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.043303718532978194	[245]	[99,160,NaN]	0	hSpCas9	negative selection	9532552
229432262	229432285	1	+	27869803	HPAFII	viability after 27 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.8375359767934596	[606]	[788,387,NaN]	6	hSpCas9	negative selection	9532553
229432563	229432586	1	-	27869803	HPAFII	viability after 27 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	0.2888827875972102	[45]	[58,4,NaN]	2	hSpCas9	negative selection	9532554
229433040	229433063	1	-	27869803	HPAFII	viability after 27 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.1808668836579348	[17]	[21,20,NaN]	8	hSpCas9	negative selection	9532555
229433041	229433064	1	-	27869803	HPAFII	viability after 27 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.5373927129615417	[74]	[150,83,NaN]	9	hSpCas9	negative selection	9532556
229431534	229431557	1	-	27869803	HPAFII	viability after 15 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.29602264903536923	[808]	[894,591,NaN]	-3	hSpCas9	negative selection	9615000
229431800	229431823	1	-	27869803	HPAFII	viability after 15 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.5829257600769717	[245]	[387,400,NaN]	6	hSpCas9	negative selection	9615001
229432262	229432285	1	+	27869803	HPAFII	viability after 15 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.7598237856595913	[606]	[1793,675,NaN]	8	hSpCas9	negative selection	9615002
229432563	229432586	1	-	27869803	HPAFII	viability after 15 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	-0.2917198109409087	[45]	[77,16,NaN]	-3	hSpCas9	negative selection	9615003
229433040	229433063	1	-	27869803	HPAFII	viability after 15 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.7893719174833036	[17]	[46,24,NaN]	8	hSpCas9	negative selection	9615004
229433041	229433064	1	-	27869803	HPAFII	viability after 15 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.2941027306975028	[74]	[328,115,NaN]	9	hSpCas9	negative selection	9615005
229431534	229431557	1	-	27869803	ASPC1	viability after 36 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.14854910223839002	[2154]	[810,875,421]	-1	hSpCas9	negative selection	9697449
229431800	229431823	1	-	27869803	ASPC1	viability after 36 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-1.0178197078616238	[798]	[59,197,142]	-7	hSpCas9	negative selection	9697450
229432262	229432285	1	+	27869803	ASPC1	viability after 36 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.37174951799521083	[1766]	[1697,295,556]	4	hSpCas9	negative selection	9697451
229432563	229432586	1	-	27869803	ASPC1	viability after 36 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	-0.28972218782244896	[82]	[77,3,0]	-3	hSpCas9	negative selection	9697452
229433040	229433063	1	-	27869803	ASPC1	viability after 36 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.8275822950288898	[71]	[9,3,24]	-7	hSpCas9	negative selection	9697453
229433041	229433064	1	-	27869803	ASPC1	viability after 36 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.2084101609992852	[165]	[19,112,228]	9	hSpCas9	negative selection	9697454
229431534	229431557	1	-	27869803	PATU8988S	viability after 35 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.3422004944994872	[1924]	[646,575,NaN]	-4	hSpCas9	negative selection	9779898
229431800	229431823	1	-	27869803	PATU8988S	viability after 35 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-1.1766045427744638	[586]	[142,65,NaN]	-8	hSpCas9	negative selection	9779899
229432262	229432285	1	+	27869803	PATU8988S	viability after 35 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.26313940573196315	[1345]	[680,619,NaN]	3	hSpCas9	negative selection	9779900
229432563	229432586	1	-	27869803	PATU8988S	viability after 35 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	2.568889631434003	[21]	[84,19,NaN]	9	hSpCas9	negative selection	9779901
229433040	229433063	1	-	27869803	PATU8988S	viability after 35 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.7991392340389813	[40]	[4,13,NaN]	-7	hSpCas9	negative selection	9779902
229433041	229433064	1	-	27869803	PATU8988S	viability after 35 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	-0.48383055132234265	[129]	[10,63,NaN]	-5	hSpCas9	negative selection	9779903
229431534	229431557	1	-	27869803	PATU8988S	viability after 31 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.2927777120806587	[1924]	[619,634,NaN]	-3	hSpCas9	negative selection	9862347
229431800	229431823	1	-	27869803	PATU8988S	viability after 31 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-0.6449873695975055	[586]	[161,133,NaN]	-6	hSpCas9	negative selection	9862348
229432262	229432285	1	+	27869803	PATU8988S	viability after 31 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.25541929799933016	[1345]	[542,764,NaN]	3	hSpCas9	negative selection	9862349
229432563	229432586	1	-	27869803	PATU8988S	viability after 31 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	2.541896384556448	[21]	[80,12,NaN]	9	hSpCas9	negative selection	9862350
229433040	229433063	1	-	27869803	PATU8988S	viability after 31 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.5073351016160461	[40]	[13,34,NaN]	6	hSpCas9	negative selection	9862351
229433041	229433064	1	-	27869803	PATU8988S	viability after 31 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	0.3227884519055195	[129]	[37,98,NaN]	4	hSpCas9	negative selection	9862352
229431534	229431557	1	-	27869803	HPAFII	viability after 35 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	0.08788157945488817	[808]	[518,340,NaN]	0	hSpCas9	negative selection	9944796
229431800	229431823	1	-	27869803	HPAFII	viability after 35 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	0.5287001112056364	[245]	[86,283,NaN]	3	hSpCas9	negative selection	9944797
229432262	229432285	1	+	27869803	HPAFII	viability after 35 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	1.052660834843117	[606]	[437,861,NaN]	7	hSpCas9	negative selection	9944798
229432563	229432586	1	-	27869803	HPAFII	viability after 35 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	-1.0063056219976922	[45]	[20,0,NaN]	-5	hSpCas9	negative selection	9944799
229433040	229433063	1	-	27869803	HPAFII	viability after 35 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.7800903288901493	[17]	[17,12,NaN]	5	hSpCas9	negative selection	9944800
229433041	229433064	1	-	27869803	HPAFII	viability after 35 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.9623764218040578	[74]	[194,94,NaN]	9	hSpCas9	negative selection	9944801
229431534	229431557	1	-	27869803	HPAFII	viability after 31 days	CACCAAGCAGGAGTACGACGAGG	ACTA1	ENSG00000143632	-0.19336994806390195	[808]	[487,297,NaN]	-1	hSpCas9	negative selection	10027245
229431800	229431823	1	-	27869803	HPAFII	viability after 31 days	GCCAACAACGTCATGTCGGGGGG	ACTA1	ENSG00000143632	-0.2029398713167942	[245]	[73,169,NaN]	-1	hSpCas9	negative selection	10027246
229432262	229432285	1	+	27869803	HPAFII	viability after 31 days	CCGCGCACCTGTGGTCACGAAGG	ACTA1	ENSG00000143632	0.6862454206212814	[606]	[517,580,NaN]	5	hSpCas9	negative selection	10027247
229432563	229432586	1	-	27869803	HPAFII	viability after 31 days	TGTCCCTCTACGCCTCCGGCAGG	ACTA1	ENSG00000143632	0.08478450947379645	[45]	[46,5,NaN]	0	hSpCas9	negative selection	10027248
229433040	229433063	1	-	27869803	HPAFII	viability after 31 days	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.3366795464092016	[17]	[36,12,NaN]	8	hSpCas9	negative selection	10027249
229433041	229433064	1	-	27869803	HPAFII	viability after 31 days	TGGTGAAAGCCGGCTTCGCCGGG	ACTA1	ENSG00000143632	1.9513194911350404	[74]	[168,155,NaN]	9	hSpCas9	negative selection	10027250
229433074	229433097	1	+	28162770	P31FUJ	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	1.0746263207778686	[159]	[395]	9	hSpCas9	negative selection	10106308
229431804	229431827	1	-	28162770	P31FUJ	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.3330744551758791	[557]	[825]	3	hSpCas9	negative selection	10106309
229433040	229433063	1	-	28162770	P31FUJ	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.7671977955856217	[1]	[3]	8	hSpCas9	negative selection	10106310
229432682	229432705	1	-	28162770	P31FUJ	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.2515387669959642	[464]	[458]	-2	hSpCas9	negative selection	10106311
229431831	229431854	1	-	28162770	P31FUJ	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-3.2328022044143783	[7]	[0]	-9	hSpCas9	negative selection	10106312
229431744	229431767	1	-	28162770	P31FUJ	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	0.8589403642131967	[98]	[210]	8	hSpCas9	negative selection	10106313
229432593	229432616	1	+	28162770	P31FUJ	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.9775926838224764	[206]	[478]	9	hSpCas9	negative selection	10106314
229432407	229432430	1	-	28162770	P31FUJ	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.03842415924752718	[103]	[118]	0	hSpCas9	negative selection	10106315
229432009	229432032	1	+	28162770	P31FUJ	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.8434735628674261	[395]	[834]	8	hSpCas9	negative selection	10106316
229431983	229432006	1	+	28162770	P31FUJ	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.3186077365957509	[231]	[339]	3	hSpCas9	negative selection	10106317
229433074	229433097	1	+	28162770	TF1	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	NaN	[NaN]	[147]	1	hSpCas9	negative selection	10275674
229431804	229431827	1	-	28162770	TF1	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	NaN	[NaN]	[541]	1	hSpCas9	negative selection	10275675
229433040	229433063	1	-	28162770	TF1	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	NaN	[NaN]	[19]	1	hSpCas9	negative selection	10275676
229432682	229432705	1	-	28162770	TF1	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	NaN	[NaN]	[348]	1	hSpCas9	negative selection	10275677
229431831	229431854	1	-	28162770	TF1	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	NaN	[NaN]	[0]	1	hSpCas9	negative selection	10275678
229431744	229431767	1	-	28162770	TF1	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	NaN	[NaN]	[105]	1	hSpCas9	negative selection	10275679
229432593	229432616	1	+	28162770	TF1	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	NaN	[NaN]	[402]	1	hSpCas9	negative selection	10275680
229432407	229432430	1	-	28162770	TF1	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	NaN	[NaN]	[173]	1	hSpCas9	negative selection	10275681
229432009	229432032	1	+	28162770	TF1	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	NaN	[NaN]	[477]	1	hSpCas9	negative selection	10275682
229431983	229432006	1	+	28162770	TF1	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	NaN	[NaN]	[135]	1	hSpCas9	negative selection	10275683
229433074	229433097	1	+	28162770	SKM1	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.8102665174775074	[39]	[123]	7	hSpCas9	negative selection	10445040
229431804	229431827	1	-	28162770	SKM1	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.5538652039761607	[125]	[326]	5	hSpCas9	negative selection	10445041
229433040	229433063	1	-	28162770	SKM1	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-1.8220016980220048	[1]	[0]	-8	hSpCas9	negative selection	10445042
229432682	229432705	1	-	28162770	SKM1	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.04000729890566879	[147]	[268]	0	hSpCas9	negative selection	10445043
229431831	229431854	1	-	28162770	SKM1	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-1.8220016980220048	[1]	[0]	-8	hSpCas9	negative selection	10445044
229431744	229431767	1	-	28162770	SKM1	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.06992921146559095	[37]	[63]	0	hSpCas9	negative selection	10445045
229432593	229432616	1	+	28162770	SKM1	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.7905730339070107	[68]	[210]	7	hSpCas9	negative selection	10445046
229432407	229432430	1	-	28162770	SKM1	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.5265458144958344	[21]	[26]	-4	hSpCas9	negative selection	10445047
229432009	229432032	1	+	28162770	SKM1	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.2966427984766138	[104]	[227]	3	hSpCas9	negative selection	10445048
229431983	229432006	1	+	28162770	SKM1	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-1.1313297561297344	[56]	[45]	-7	hSpCas9	negative selection	10445049
229433074	229433097	1	+	28162770	SKM1	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.8102665174775074	[39]	[123]	7	hSpCas9	negative selection	10614406
229431804	229431827	1	-	28162770	SKM1	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.5538652039761607	[125]	[326]	5	hSpCas9	negative selection	10614407
229433040	229433063	1	-	28162770	SKM1	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-1.8220016980220048	[1]	[0]	-8	hSpCas9	negative selection	10614408
229432682	229432705	1	-	28162770	SKM1	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.04000729890566879	[147]	[268]	0	hSpCas9	negative selection	10614409
229431831	229431854	1	-	28162770	SKM1	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-1.8220016980220048	[1]	[0]	-8	hSpCas9	negative selection	10614410
229431744	229431767	1	-	28162770	SKM1	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.06992921146559095	[37]	[63]	0	hSpCas9	negative selection	10614411
229432593	229432616	1	+	28162770	SKM1	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.7905730339070107	[68]	[210]	7	hSpCas9	negative selection	10614412
229432407	229432430	1	-	28162770	SKM1	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.5265458144958344	[21]	[26]	-4	hSpCas9	negative selection	10614413
229432009	229432032	1	+	28162770	SKM1	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.2966427984766138	[104]	[227]	3	hSpCas9	negative selection	10614414
229431983	229432006	1	+	28162770	SKM1	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-1.1313297561297344	[56]	[45]	-7	hSpCas9	negative selection	10614415
229433074	229433097	1	+	28162770	PL21	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	-0.14225258295775783	[141]	[55]	-1	hSpCas9	negative selection	10783772
229431804	229431827	1	-	28162770	PL21	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.23717699381384993	[422]	[216]	2	hSpCas9	negative selection	10783773
229433040	229433063	1	-	28162770	PL21	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.7258598040669031	[18]	[4]	-5	hSpCas9	negative selection	10783774
229432682	229432705	1	-	28162770	PL21	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.3234223415676927	[666]	[231]	-2	hSpCas9	negative selection	10783775
229431831	229431854	1	-	28162770	PL21	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	1.5220677093766826	[3]	[4]	9	hSpCas9	negative selection	10783776
229431744	229431767	1	-	28162770	PL21	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-1.0744827656196854	[149]	[30]	-6	hSpCas9	negative selection	10783777
229432593	229432616	1	+	28162770	PL21	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	-0.12178848039804221	[184]	[73]	-1	hSpCas9	negative selection	10783778
229432407	229432430	1	-	28162770	PL21	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	0.6382607268812053	[61]	[41]	5	hSpCas9	negative selection	10783779
229432009	229432032	1	+	28162770	PL21	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	-0.2836106348735379	[343]	[122]	-2	hSpCas9	negative selection	10783780
229431983	229432006	1	+	28162770	PL21	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.05718166064727695	[158]	[71]	0	hSpCas9	negative selection	10783781
229433074	229433097	1	+	28162770	P31FUJ	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	1.0746263207778686	[159]	[395]	9	hSpCas9	negative selection	10953138
229431804	229431827	1	-	28162770	P31FUJ	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.3330744551758791	[557]	[825]	3	hSpCas9	negative selection	10953139
229433040	229433063	1	-	28162770	P31FUJ	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.7671977955856217	[1]	[3]	8	hSpCas9	negative selection	10953140
229432682	229432705	1	-	28162770	P31FUJ	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.2515387669959642	[464]	[458]	-2	hSpCas9	negative selection	10953141
229431831	229431854	1	-	28162770	P31FUJ	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-3.2328022044143783	[7]	[0]	-9	hSpCas9	negative selection	10953142
229431744	229431767	1	-	28162770	P31FUJ	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	0.8589403642131967	[98]	[210]	8	hSpCas9	negative selection	10953143
229432593	229432616	1	+	28162770	P31FUJ	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.9775926838224764	[206]	[478]	9	hSpCas9	negative selection	10953144
229432407	229432430	1	-	28162770	P31FUJ	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.03842415924752718	[103]	[118]	0	hSpCas9	negative selection	10953145
229432009	229432032	1	+	28162770	P31FUJ	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.8434735628674261	[395]	[834]	8	hSpCas9	negative selection	10953146
229431983	229432006	1	+	28162770	P31FUJ	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.3186077365957509	[231]	[339]	3	hSpCas9	negative selection	10953147
229433074	229433097	1	+	28162770	OCIAML5	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.01960933216046362	[93]	[171]	0	hSpCas9	negative selection	11122504
229431804	229431827	1	-	28162770	OCIAML5	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.6465023060580348	[280]	[793]	5	hSpCas9	negative selection	11122505
229433040	229433063	1	-	28162770	OCIAML5	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-1.8520665708639967	[1]	[0]	-7	hSpCas9	negative selection	11122506
229432682	229432705	1	-	28162770	OCIAML5	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.09389358942311388	[203]	[392]	0	hSpCas9	negative selection	11122507
229431831	229431854	1	-	28162770	OCIAML5	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	1.7328959298571593	[0]	[5]	9	hSpCas9	negative selection	11122508
229431744	229431767	1	-	28162770	OCIAML5	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.7317723371462852	[68]	[74]	-5	hSpCas9	negative selection	11122509
229432593	229432616	1	+	28162770	OCIAML5	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.5072149385057351	[121]	[312]	4	hSpCas9	negative selection	11122510
229432407	229432430	1	-	28162770	OCIAML5	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.37672856131733923	[40]	[56]	-3	hSpCas9	negative selection	11122511
229432009	229432032	1	+	28162770	OCIAML5	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.5335871216337525	[185]	[485]	4	hSpCas9	negative selection	11122512
229431983	229432006	1	+	28162770	OCIAML5	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-0.16285410814615892	[128]	[207]	-1	hSpCas9	negative selection	11122513
229433074	229433097	1	+	28162770	EOL1	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	-0.36871656506468414	[298]	[121]	-3	hSpCas9	negative selection	11291870
229431804	229431827	1	-	28162770	EOL1	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.6541499773232542	[549]	[455]	6	hSpCas9	negative selection	11291871
229433040	229433063	1	-	28162770	EOL1	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.9823428240379837	[14]	[3]	-6	hSpCas9	negative selection	11291872
229432682	229432705	1	-	28162770	EOL1	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.03156458442846555	[1035]	[533]	0	hSpCas9	negative selection	11291873
229431831	229431854	1	-	28162770	EOL1	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-0.07545222842946586	[5]	[2]	0	hSpCas9	negative selection	11291874
229431744	229431767	1	-	28162770	EOL1	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	0.10219068617368372	[289]	[163]	0	hSpCas9	negative selection	11291875
229432593	229432616	1	+	28162770	EOL1	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	1.4738863625609695	[245]	[359]	9	hSpCas9	negative selection	11291876
229432407	229432430	1	-	28162770	EOL1	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.12125591804259006	[63]	[30]	-1	hSpCas9	negative selection	11291877
229432009	229432032	1	+	28162770	EOL1	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.9122345101316831	[352]	[349]	7	hSpCas9	negative selection	11291878
229431983	229432006	1	+	28162770	EOL1	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-0.22130309489292022	[207]	[93]	-2	hSpCas9	negative selection	11291879
229433074	229433097	1	+	28162770	MOLM13	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	1.8697699776197176	[178]	[472]	9	hSpCas9	negative selection	11461236
229431804	229431827	1	-	28162770	MOLM13	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	-0.23631225539271228	[623]	[382]	-1	hSpCas9	negative selection	11461237
229433040	229433063	1	-	28162770	MOLM13	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.9471481177342644	[7]	[2]	-5	hSpCas9	negative selection	11461238
229432682	229432705	1	-	28162770	MOLM13	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.7087552177041018	[806]	[356]	-4	hSpCas9	negative selection	11461239
229431831	229431854	1	-	28162770	MOLM13	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-0.5321106184554214	[1]	[0]	-3	hSpCas9	negative selection	11461240
229431744	229431767	1	-	28162770	MOLM13	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.1407521156460878	[214]	[140]	-1	hSpCas9	negative selection	11461241
229432593	229432616	1	+	28162770	MOLM13	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	1.641004070425689	[250]	[565]	9	hSpCas9	negative selection	11461242
229432407	229432430	1	-	28162770	MOLM13	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-1.5660579503787588	[85]	[20]	-6	hSpCas9	negative selection	11461243
229432009	229432032	1	+	28162770	MOLM13	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.05795877269882366	[375]	[282]	0	hSpCas9	negative selection	11461244
229431983	229432006	1	+	28162770	MOLM13	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.6175542329204278	[246]	[273]	4	hSpCas9	negative selection	11461245
229433074	229433097	1	+	28162770	HEL	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.9566741361341888	[180]	[224]	8	hSpCas9	negative selection	11630602
229431804	229431827	1	-	28162770	HEL	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.7171636731269115	[509]	[536]	6	hSpCas9	negative selection	11630603
229433040	229433063	1	-	28162770	HEL	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	0.7682697140842158	[10]	[11]	6	hSpCas9	negative selection	11630604
229432682	229432705	1	-	28162770	HEL	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	0.5175970787228732	[673]	[617]	4	hSpCas9	negative selection	11630605
229431831	229431854	1	-	28162770	HEL	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-0.942223668720799	[2]	[0]	-5	hSpCas9	negative selection	11630606
229431744	229431767	1	-	28162770	HEL	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.17785191721394117	[218]	[123]	-1	hSpCas9	negative selection	11630607
229432593	229432616	1	+	28162770	HEL	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	1.449116649353568	[210]	[368]	9	hSpCas9	negative selection	11630608
229432407	229432430	1	-	28162770	HEL	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.8520258597492207	[61]	[21]	-5	hSpCas9	negative selection	11630609
229432009	229432032	1	+	28162770	HEL	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.28588240381004243	[323]	[252]	2	hSpCas9	negative selection	11630610
229431983	229432006	1	+	28162770	HEL	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.3494466050738826	[211]	[172]	3	hSpCas9	negative selection	11630611
229433074	229433097	1	+	28162770	MV411	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.6137092230663556	[172]	[222]	5	hSpCas9	negative selection	11799968
229431804	229431827	1	-	28162770	MV411	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.6636343068864596	[310]	[414]	6	hSpCas9	negative selection	11799969
229433040	229433063	1	-	28162770	MV411	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-2.074490544104587	[4]	[0]	-8	hSpCas9	negative selection	11799970
229432682	229432705	1	-	28162770	MV411	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.7525624492172246	[589]	[294]	-5	hSpCas9	negative selection	11799971
229431831	229431854	1	-	28162770	MV411	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	0.24743755078277552	[0]	[0]	2	hSpCas9	negative selection	11799972
229431744	229431767	1	-	28162770	MV411	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	0.6163048686286934	[126]	[163]	5	hSpCas9	negative selection	11799973
229432593	229432616	1	+	28162770	MV411	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.0903938406371953	[222]	[199]	0	hSpCas9	negative selection	11799974
229432407	229432430	1	-	28162770	MV411	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	0.0007975831996498428	[69]	[58]	0	hSpCas9	negative selection	11799975
229432009	229432032	1	+	28162770	MV411	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.47855677653980694	[258]	[303]	4	hSpCas9	negative selection	11799976
229431983	229432006	1	+	28162770	MV411	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-0.25332982252195235	[207]	[146]	-2	hSpCas9	negative selection	11799977
229433074	229433097	1	+	28162770	MONOMAC1	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.575157235417634	[88]	[151]	5	hSpCas9	negative selection	11969334
229431804	229431827	1	-	28162770	MONOMAC1	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	0.20786627508557687	[249]	[330]	2	hSpCas9	negative selection	11969335
229433040	229433063	1	-	28162770	MONOMAC1	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-1.366961848501866	[8]	[3]	-8	hSpCas9	negative selection	11969336
229432682	229432705	1	-	28162770	MONOMAC1	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.18908809394406245	[361]	[363]	-2	hSpCas9	negative selection	11969337
229431831	229431854	1	-	28162770	MONOMAC1	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	1.4659281656628758	[5]	[18]	9	hSpCas9	negative selection	11969338
229431744	229431767	1	-	28162770	MONOMAC1	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-0.7653206066340797	[85]	[57]	-6	hSpCas9	negative selection	11969339
229432593	229432616	1	+	28162770	MONOMAC1	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.3366451487179092	[104]	[151]	3	hSpCas9	negative selection	11969340
229432407	229432430	1	-	28162770	MONOMAC1	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	1.8029631529404462	[27]	[111]	9	hSpCas9	negative selection	11969341
229432009	229432032	1	+	28162770	MONOMAC1	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.9634278251336924	[203]	[455]	8	hSpCas9	negative selection	11969342
229431983	229432006	1	+	28162770	MONOMAC1	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	0.2623947715777436	[119]	[164]	2	hSpCas9	negative selection	11969343
229433074	229433097	1	+	28162770	OCIAML2	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	0.9285412685776615	[19]	[210]	8	hSpCas9	negative selection	12138700
229431804	229431827	1	-	28162770	OCIAML2	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	-0.22868683314216298	[88]	[420]	-2	hSpCas9	negative selection	12138701
229433040	229433063	1	-	28162770	OCIAML2	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	1.7772976882014244	[2]	[56]	9	hSpCas9	negative selection	12138702
229432682	229432705	1	-	28162770	OCIAML2	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-1.0602237746949634	[116]	[310]	-6	hSpCas9	negative selection	12138703
229431831	229431854	1	-	28162770	OCIAML2	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-0.7336642310759549	[2]	[9]	-5	hSpCas9	negative selection	12138704
229431744	229431767	1	-	28162770	OCIAML2	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	-1.2168732329963785	[25]	[61]	-7	hSpCas9	negative selection	12138705
229432593	229432616	1	+	28162770	OCIAML2	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	0.010496864494455298	[35]	[200]	0	hSpCas9	negative selection	12138706
229432407	229432430	1	-	28162770	OCIAML2	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	-0.6980403213452337	[11]	[40]	-5	hSpCas9	negative selection	12138707
229432009	229432032	1	+	28162770	OCIAML2	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.13878571920353402	[38]	[237]	1	hSpCas9	negative selection	12138708
229431983	229432006	1	+	28162770	OCIAML2	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-0.37301902861573893	[24]	[106]	-3	hSpCas9	negative selection	12138709
229433074	229433097	1	+	28162770	OCIAML3	viability	ATTGTCGCACACGAGGGCGGTGG	ACTA1	ENSG00000143632	-0.2535832018194114	[61]	[156]	-2	hSpCas9	negative selection	12308066
229431804	229431827	1	-	28162770	OCIAML3	viability	GTATGCCAACAACGTCATGTCGG	ACTA1	ENSG00000143632	-0.0024724856795206307	[145]	[439]	0	hSpCas9	negative selection	12308067
229433040	229433063	1	-	28162770	OCIAML3	viability	GGTGAAAGCCGGCTTCGCCGGGG	ACTA1	ENSG00000143632	-0.594007640324163	[0]	[1]	-4	hSpCas9	negative selection	12308068
229432682	229432705	1	-	28162770	OCIAML3	viability	GCACCCCACCCTGCTCACCGAGG	ACTA1	ENSG00000143632	-0.6655609013491971	[154]	[294]	-5	hSpCas9	negative selection	12308069
229431831	229431854	1	-	28162770	OCIAML3	viability	GTGTGACATCGACATCAGGAAGG	ACTA1	ENSG00000143632	-1.594007640324163	[0]	[0]	-8	hSpCas9	negative selection	12308070
229431744	229431767	1	-	28162770	OCIAML3	viability	GCAGAAAGAGATCACCGCGCTGG	ACTA1	ENSG00000143632	0.30411274565662305	[43]	[163]	3	hSpCas9	negative selection	12308071
229432593	229432616	1	+	28162770	OCIAML3	viability	CTGGATGGCCACGTACATGGCGG	ACTA1	ENSG00000143632	-0.2314375609394549	[83]	[215]	-2	hSpCas9	negative selection	12308072
229432407	229432430	1	-	28162770	OCIAML3	viability	ATCGTGCTGGACTCCGGCGACGG	ACTA1	ENSG00000143632	0.5557394791805188	[15]	[70]	5	hSpCas9	negative selection	12308073
229432009	229432032	1	+	28162770	OCIAML3	viability	GGAAGAGCGTCTCCGGGCAGCGG	ACTA1	ENSG00000143632	0.27388882366849154	[79]	[291]	2	hSpCas9	negative selection	12308074
229431983	229432006	1	+	28162770	OCIAML3	viability	GCGGGGCTCACCGATGAAGGAGG	ACTA1	ENSG00000143632	-1.4305089080412838	[49]	[55]	-7	hSpCas9	negative selection	12308075