Gene2Function

Crispr

Gene Info

Species: Human (Homo sapiens)
GeneID: 85476
Symbol: GFM1
Description: G elongation factor mitochondrial 1

DataSource: http://genomecrispr.dkfz.de/#!/results/GFM1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
158684550	158684573	3	-	26472758	Jiyoye	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-0.9510162573682607	[140]	[54]	-6	hSpCas9	negative selection	62817
158690249	158690272	3	+	26472758	Jiyoye	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-0.27871602806606355	[110]	[68]	-2	hSpCas9	negative selection	62818
158684550	158684573	3	-	26472758	KBM7	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-1.715102964435716	[447,298]	[77,29]	-8	hSpCas9	negative selection	253935
158690249	158690272	3	+	26472758	KBM7	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-1.982646454350174	[781,429]	[73,66]	-8	hSpCas9	negative selection	253936
158684550	158684573	3	-	26472758	Raji	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-2.148956217886186	[171]	[8]	-8	hSpCas9	negative selection	445053
158690249	158690272	3	+	26472758	Raji	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-0.7110690955673353	[260]	[36]	-5	hSpCas9	negative selection	445054
158684550	158684573	3	-	26472758	K562	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-1.8357516210175362	[295]	[69]	-7	hSpCas9	negative selection	636171
158690249	158690272	3	+	26472758	K562	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-2.0115262533810276	[405]	[84]	-7	hSpCas9	negative selection	636172
158644685	158644708	3	-	26627737	DLD1	viability	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-2.000589612083351	[579]	[12,64,56]	-8	hSpCas9	negative selection	820238
158645630	158645653	3	+	26627737	DLD1	viability	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-4.8615002801799125	[92]	[0,0,0]	-9	hSpCas9	negative selection	820239
158644685	158644708	3	-	26627737	GBM cells	viability after 5 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.16083466040877098	[215]	[137,120]	2	hSpCas9	negative selection	902553
158645630	158645653	3	+	26627737	GBM cells	viability after 5 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-0.8046931223340894	[56]	[27,6]	-8	hSpCas9	negative selection	902554
158644685	158644708	3	-	26627737	GBM cells	viability after 13 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-0.38270037510840205	[215]	[74,101]	-4	hSpCas9	negative selection	984868
158645630	158645653	3	+	26627737	GBM cells	viability after 13 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-1.9952224482420862	[56]	[4,9]	-9	hSpCas9	negative selection	984869
158644685	158644708	3	-	26627737	GBM cells	viability after 21 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-1.2120056050127197	[215]	[46,53]	-7	hSpCas9	negative selection	1067183
158645630	158645653	3	+	26627737	GBM cells	viability after 21 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-1.2472931177687059	[56]	[11,13]	-8	hSpCas9	negative selection	1067184
158644685	158644708	3	-	26627737	RPE1	viability after 9 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.5769235613793383	[446]	[175,250]	4	hSpCas9	negative selection	1149498
158645630	158645653	3	+	26627737	RPE1	viability after 9 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	6.516512489963747	[0]	[15,44]	9	hSpCas9	negative selection	1149499
158644685	158644708	3	-	26627737	RPE1	viability after 12 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.023393305232407346	[446]	[106,137]	0	hSpCas9	negative selection	1231813
158645630	158645653	3	+	26627737	RPE1	viability after 12 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	4.042952800002409	[0]	[1,6]	9	hSpCas9	negative selection	1231814
158644685	158644708	3	-	26627737	RPE1	viability after 15 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-0.39341319524216745	[446]	[24,164]	-2	hSpCas9	negative selection	1314128
158645630	158645653	3	+	26627737	RPE1	viability after 15 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	4.148889964642173	[0]	[3,5]	9	hSpCas9	negative selection	1314129
158644685	158644708	3	-	26627737	RPE1	viability after 18 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-0.031761768670306	[446]	[88,148]	0	hSpCas9	negative selection	1396443
158645630	158645653	3	+	26627737	RPE1	viability after 18 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	5.172789537711752	[0]	[1,17]	9	hSpCas9	negative selection	1396444
158644685	158644708	3	-	26627737	HeLa	viability after 8 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.07697721916406391	[308]	[77,288,50]	0	hSpCas9	negative selection	1478758
158645630	158645653	3	+	26627737	HeLa	viability after 8 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	0.711521157831783	[25]	[52,3,6]	6	hSpCas9	negative selection	1478759
158644685	158644708	3	-	26627737	HeLa	viability after 12 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-0.5891356924768794	[308]	[106,49,101]	-5	hSpCas9	negative selection	1561073
158645630	158645653	3	+	26627737	HeLa	viability after 12 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-0.3448406627887364	[25]	[2,19,0]	-3	hSpCas9	negative selection	1561074
158644685	158644708	3	-	26627737	HeLa	viability after 15 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-1.0944010437680454	[308]	[16,140,15]	-7	hSpCas9	negative selection	1643388
158645630	158645653	3	+	26627737	HeLa	viability after 15 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	1.362460777941326	[25]	[76,0,0]	8	hSpCas9	negative selection	1643389
158644685	158644708	3	-	26627737	HeLa	viability after 18 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-1.0401383003206641	[308]	[9,123,15]	-7	hSpCas9	negative selection	1725703
158645630	158645653	3	+	26627737	HeLa	viability after 18 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	1.1726285312161546	[25]	[44,0,0]	8	hSpCas9	negative selection	1725704
158644685	158644708	3	-	26627737	HCT116	viability after 6 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.11332748218902794	[522]	[193,30]	0	hSpCas9	negative selection	1808018
158645630	158645653	3	+	26627737	HCT116	viability after 6 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	1.5710263613391335	[53]	[21,26]	7	hSpCas9	negative selection	1808019
158644685	158644708	3	-	26627737	HCT116	viability after 8 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.8653465325042025	[125]	[42,128,204]	7	hSpCas9	negative selection	1890333
158645630	158645653	3	+	26627737	HCT116	viability after 8 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	0.7262055460867591	[22]	[23,21,16]	7	hSpCas9	negative selection	1890334
158644685	158644708	3	-	26627737	HCT116	viability after 9 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-0.9483700300668183	[522]	[106,44]	-6	hSpCas9	negative selection	1972648
158645630	158645653	3	+	26627737	HCT116	viability after 9 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-3.84989769081	[53]	[0,0]	-9	hSpCas9	negative selection	1972649
158644685	158644708	3	-	26627737	HCT116	viability after 12 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.5463109891485181	[125]	[61,131,99]	5	hSpCas9	negative selection	2054963
158645630	158645653	3	+	26627737	HCT116	viability after 12 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	0.45310824110687964	[22]	[8,3,36]	4	hSpCas9	negative selection	2054964
158644685	158644708	3	-	26627737	HCT116	viability after 15 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.6731311447077666	[125]	[45,117,172]	6	hSpCas9	negative selection	2137278
158645630	158645653	3	+	26627737	HCT116	viability after 15 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-1.0003307505091525	[22]	[0,0,16]	-7	hSpCas9	negative selection	2137279
158644685	158644708	3	-	26627737	HCT116	viability after 18 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	0.07710228338317049	[125]	[39,97,51]	0	hSpCas9	negative selection	2219593
158645630	158645653	3	+	26627737	HCT116	viability after 18 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-3.475009580401591	[22]	[0,0,0]	-9	hSpCas9	negative selection	2219594
158672849	158672872	3	+	25494202	A375	resistance to PLX-4720 (puromycin)	ATTAACCTTTATAGAAGTGCAGG	GFM1	ENSG00000168827	2.1888077185506876	[74,173]	[26,233]	7	dCas9-VP64	positive selection	2451377
158672795	158672818	3	+	25494202	A375	resistance to PLX-4720 (puromycin)	GGTGTGGGGAGGGAGTCAGTGGG	GFM1	ENSG00000168827	-1.112864874926568	[9,299]	[3,27]	-6	dCas9-VP64	positive selection	2451378
158672774	158672797	3	+	25494202	A375	resistance to PLX-4720 (puromycin)	TGTCAGGCACCAGCAGGTGGAGG	GFM1	ENSG00000168827	1.3429517095508876	[2,12]	[0,6]	5	dCas9-VP64	positive selection	2451379
158644359	158644382	3	-	25494202	A375	resistance to PLX-4720 (puromycin)	AGTGTTTGCTCCCAGTAAGACGG	GFM1	ENSG00000168827	-0.7644962931960313	[50,802]	[9,105]	-4	dCas9-VP64	positive selection	2456882
158644335	158644358	3	-	25494202	A375	resistance to PLX-4720 (puromycin)	ATAGGTATATGGGTGAGGGAAGG	GFM1	ENSG00000168827	2.987074716099135	[106,15]	[167,39]	8	dCas9-VP64	positive selection	2456883
158644402	158644425	3	+	25494202	A375	resistance to PLX-4720 (puromycin)	TTGCACTTTCTCTTCCAAGTCGG	GFM1	ENSG00000168827	-0.08121090891207533	[125,113]	[6,43]	0	dCas9-VP64	positive selection	2456884
158672849	158672872	3	+	25494202	A375	resistance to PLX-4720 (zeocin)	ATTAACCTTTATAGAAGTGCAGG	GFM1	ENSG00000168827	1.362000686654543	[112,168]	[156,80]	7	dCas9-VP64	positive selection	2546632
158672795	158672818	3	+	25494202	A375	resistance to PLX-4720 (zeocin)	GGTGTGGGGAGGGAGTCAGTGGG	GFM1	ENSG00000168827	0.23780804807272765	[76,109]	[27,42]	2	dCas9-VP64	positive selection	2546633
158672774	158672797	3	+	25494202	A375	resistance to PLX-4720 (zeocin)	TGTCAGGCACCAGCAGGTGGAGG	GFM1	ENSG00000168827	0.4395741808250242	[63,44]	[15,30]	3	dCas9-VP64	positive selection	2546634
158644359	158644382	3	-	25494202	A375	resistance to PLX-4720 (zeocin)	AGTGTTTGCTCCCAGTAAGACGG	GFM1	ENSG00000168827	-0.6154184862598697	[101,151]	[26,25]	-6	dCas9-VP64	positive selection	2552137
158644335	158644358	3	-	25494202	A375	resistance to PLX-4720 (zeocin)	ATAGGTATATGGGTGAGGGAAGG	GFM1	ENSG00000168827	-0.30075641536172687	[144,195]	[39,48]	-3	dCas9-VP64	positive selection	2552138
158644402	158644425	3	+	25494202	A375	resistance to PLX-4720 (zeocin)	TTGCACTTTCTCTTCCAAGTCGG	GFM1	ENSG00000168827	0.5679193197949761	[87,585]	[26,285]	4	dCas9-VP64	positive selection	2552139
158645737	158645760		+	24336571	A375	resistance to PLX after 7 days	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	0.4518101164720459	[6,6]	[7,9]	9	hSpCas9	positive selection	3116384
158645737	158645760		+	24336571	A375	resistance to PLX after 14 days	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	0.616138801994322	[4,3]	[3,1]	5	hSpCas9	positive selection	3174177
158645737	158645760		+	24336571	A375	viability after 7 days	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.17138990499195983	[7]	[6,6]	-2	hSpCas9	negative selection	3231970
158645737	158645760		+	24336571	A375	viability after 14 days	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.738256406437545	[7]	[4,3]	-7	hSpCas9	negative selection	3289763
158645737	158645760	3	+	27383988	293T	resistance to West Nile virus (flavivirus)	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	0.13811381519134064	[142,134]	[0,0]	0	hSpCas9	positive selection	3351348
158645642	158645665	3	+	27383988	293T	resistance to West Nile virus (flavivirus)	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.446509523142357	[206,209]	[0,0]	-3	hSpCas9	positive selection	3351349
158645642	158645665	3	+	26780180	A375	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	1.28194458781377	[]	[]	7	hSpCas9	negative selection	3943560
158645737	158645760	3	+	26780180	A375	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	1.58223103906254	[]	[]	8	hSpCas9	negative selection	3943561
158645642	158645665	3	+	26780180	HT29	viability (GeCKOv2 library)	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.185287883382305	[]	[]	0	hSpCas9	negative selection	4051526
158645737	158645760	3	+	26780180	HT29	viability (GeCKOv2 library)	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	3.06388146155558	[]	[]	9	hSpCas9	negative selection	4051527
158645737	158645760	3	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.4884645057749192	[3]	[0,1]	-8	hSpCas9	positive selection	4139565
158645737	158645760	3	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.26383359301825493	[1]	[0,0,0,0]	-4	hSpCas9	positive selection	4203587
158645737	158645760	3	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.29342914373665496	[3]	[2,1]	-6	hSpCas9	positive selection	4272632
158645642	158645665	3	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.5359635958372049	[3]	[4,4]	9	hSpCas9	positive selection	4272634
158645738	158645760	3	+	27760321	OCIAML3	viability	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.8216825288921537	[106,94]	[51,34]	-6	hSpCas9	negative selection	4403427
158645738	158645760	3	+	27760321	OCIAML2	viability	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-2.9643115494170544	[106,94]	[6,14]	-9	hSpCas9	negative selection	4486890
158645738	158645760	3	+	27760321	MV411	viability	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-4.678458413169107	[106,94]	[0,4]	-9	hSpCas9	negative selection	4570353
158645738	158645760	3	+	27760321	HL60	viability	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-6.364103847535739	[106,94]	[0,0]	-9	hSpCas9	negative selection	4653816
158645738	158645760	3	+	27760321	HT1080	viability	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-5.0728887527159365	[94,73]	[0,0]	-8	hSpCas9	negative selection	4737279
158645738	158645760	3	+	27760321	HT29	viability after 7 days	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.21949152401939065	[94,73]	[91,75,110]	-3	hSpCas9	negative selection	4820742
158645738	158645760	3	+	27760321	HT29	viability after 25 days	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-6.592571017562461	[94,73]	[0,0,0]	-9	hSpCas9	negative selection	4904205
158645738	158645760	3	+	27760321	HT29	viability after 22 days	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-2.8578219472585897	[94,73]	[0,0,38]	-8	hSpCas9	negative selection	4987668
158645738	158645760	3	+	27760321	HT29	viability after 19 days	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-5.443111015895045	[94,73]	[4,0,0]	-9	hSpCas9	negative selection	5071131
158645738	158645760	3	+	27760321	HT29	viability after 16 days	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.742113796475155	[94,73]	[81,15,74]	-6	hSpCas9	negative selection	5154594
158645738	158645760	3	+	27760321	HT29	viability after 13 days	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.19855780569803505	[94,73]	[110,90,55]	-2	hSpCas9	negative selection	5238057
158645738	158645760	3	+	27760321	HT29	viability after 10 days	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.4093737087023839	[94,73]	[27,116,93]	-5	hSpCas9	negative selection	5321520
158645738	158645760	3	+	27760321	MOLM13	viability	AACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-1.7873637893750343	[106,94]	[9,21]	-7	hSpCas9	negative selection	5404983
158644626	158644649	3	+	27661255	K562	viability	GCGCTTGCCATGAGACTCCTGGG	GFM1	ENSG00000168827	-0.6629076115441701	[288,337]	[174,142]	-8	dCas9-KRAB	negative selection	5481949
158644712	158644735	3	+	27661255	K562	viability	GCAGGTACCGGAGCATAGAGAGG	GFM1	ENSG00000168827	-0.7758271199881208	[1611,1638]	[850,678]	-8	dCas9-KRAB	negative selection	5481950
158644546	158644569	3	-	27661255	K562	viability	GGAAGCGGTCAAAGTCACGCCGG	GFM1	ENSG00000168827	-1.295232621269618	[1369,1307]	[507,374]	-9	dCas9-KRAB	negative selection	5481951
158644602	158644625	3	-	27661255	K562	viability	GAGCACGCGTCAAAGTCCCGTGG	GFM1	ENSG00000168827	-2.4970263022295454	[978,1004]	[142,137]	-9	dCas9-KRAB	negative selection	5481952
158672703	158672726	3	-	27661255	K562	viability	GCCCGCTGCAGGCAGTAGTGGGG	GFM1	ENSG00000168827	0.07311274485023822	[414,392]	[321,354]	2	dCas9-KRAB	negative selection	5491401
158645737	158645760	3	+	27260156	BXPC3	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.31428270641221295	[627]	[481,525,550,558]	-6	hSpCas9	negative selection	5545092
158645642	158645665	3	+	27260156	BXPC3	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.0843433812146468	[845]	[810,748,921,861]	-2	hSpCas9	negative selection	5545094
158645737	158645760	3	+	27260156	A673	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.10757731546810129	[627]	[555,729,747,748]	-3	hSpCas9	negative selection	5666343
158645642	158645665	3	+	27260156	A673	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.3097735945332548	[845]	[1339,1109,1212,1391]	7	hSpCas9	negative selection	5666345
158645737	158645760	3	+	27260156	A375	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.7106173781124573	[627]	[551,522,322,408]	-7	hSpCas9	negative selection	5787594
158645642	158645665	3	+	27260156	A375	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.5658186628596726	[845]	[662,888,678,488]	-6	hSpCas9	negative selection	5787596
158645737	158645760	3	+	27260156	COLO741	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.21283686251239092	[627]	[742,662,674]	-4	hSpCas9	negative selection	5908845
158645642	158645665	3	+	27260156	COLO741	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.09777075173109806	[845]	[1193,1193,1087]	2	hSpCas9	negative selection	5908847
158645737	158645760	3	+	27260156	CAL120	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.5704158877987798	[627]	[490,652,430]	-7	hSpCas9	negative selection	6030096
158645642	158645665	3	+	27260156	CAL120	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.1961879569495134	[845]	[1124,1631,854]	3	hSpCas9	negative selection	6030098
158645737	158645760	3	+	27260156	CADOES1	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.327384686908487	[627]	[462,367,482,550]	-5	hSpCas9	negative selection	6151347
158645642	158645665	3	+	27260156	CADOES1	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.17247670444593388	[845]	[543,687,811,750]	-3	hSpCas9	negative selection	6151349
158645737	158645760	3	+	27260156	EWS502	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.7085235908188569	[627]	[392,585,289,345]	-8	hSpCas9	negative selection	6272598
158645642	158645665	3	+	27260156	EWS502	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.1945363692259946	[845]	[1124,748,1059,1173]	3	hSpCas9	negative selection	6272600
158645737	158645760	3	+	27260156	EW8	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.32821466412565503	[627]	[443,650,270,282]	-6	hSpCas9	negative selection	6393849
158645642	158645665	3	+	27260156	EW8	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.012648804650757461	[845]	[897,578,495,797]	0	hSpCas9	negative selection	6393851
158645737	158645760	3	+	27260156	CORL105	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.8660339064592661	[627]	[328,381,460,447]	-9	hSpCas9	negative selection	6515100
158645642	158645665	3	+	27260156	CORL105	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.4136302743198617	[845]	[875,556,700,840]	-6	hSpCas9	negative selection	6515102
158645737	158645760	3	+	27260156	HS294T	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.4124787984912487	[627]	[269,119,158,171]	-5	hSpCas9	negative selection	6636351
158645642	158645665	3	+	27260156	HS294T	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.21757867405056885	[845]	[342,260,437,506]	3	hSpCas9	negative selection	6636353
158645737	158645760	3	+	27260156	HCC44	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.6778328996957648	[627]	[590,449,330,394]	-7	hSpCas9	negative selection	6757602
158645642	158645665	3	+	27260156	HCC44	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.23581012224659392	[845]	[1339,979,1120,1096]	4	hSpCas9	negative selection	6757604
158645737	158645760	3	+	27260156	G402	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.7118493267972259	[627]	[820,317,519,262]	-7	hSpCas9	negative selection	6878853
158645642	158645665	3	+	27260156	G402	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.0010573531499611377	[845]	[1623,740,734,1087]	0	hSpCas9	negative selection	6878855
158645737	158645760	3	+	27260156	L33	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.3452676373975092	[627]	[464,197,348,474]	-7	hSpCas9	negative selection	7000104
158645642	158645665	3	+	27260156	L33	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.2601338862866768	[845]	[1166,415,570,957]	5	hSpCas9	negative selection	7000106
158645737	158645760	3	+	27260156	K562	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.9702055866095982	[627]	[530,278,188,170]	-8	hSpCas9	negative selection	7121355
158645642	158645665	3	+	27260156	K562	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.06016079739878066	[845]	[437,1284,414,965]	0	hSpCas9	negative selection	7121357
158645737	158645760	3	+	27260156	HT29	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.7513783902736131	[627]	[385,521,401,495]	-8	hSpCas9	negative selection	7242606
158645642	158645665	3	+	27260156	HT29	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.13148351510565903	[845]	[884,986,1130,766]	-3	hSpCas9	negative selection	7242608
158645737	158645760	3	+	27260156	MHHES1	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.4199263196552729	[627]	[411,582,544,548]	-7	hSpCas9	negative selection	7363857
158645642	158645665	3	+	27260156	MHHES1	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.23414589620479387	[845]	[1291,1359,1135,791]	4	hSpCas9	negative selection	7363859
158645737	158645760	3	+	27260156	MEWO	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.12348137823367294	[627]	[629,632,371]	-3	hSpCas9	negative selection	7485108
158645642	158645665	3	+	27260156	MEWO	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.22558446172629665	[845]	[861,891,1009]	5	hSpCas9	negative selection	7485110
158645737	158645760	3	+	27260156	LNCAPCLONEFGC	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.36512797614955755	[627]	[532,402,399,424]	-5	hSpCas9	negative selection	7606359
158645642	158645665	3	+	27260156	LNCAPCLONEFGC	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.04653723967713541	[845]	[912,632,731,691]	0	hSpCas9	negative selection	7606361
158645737	158645760	3	+	27260156	PANC0327	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.34315973662048266	[627]	[479,438,289,552]	-5	hSpCas9	negative selection	7727610
158645642	158645665	3	+	27260156	PANC0327	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.08389970344792252	[845]	[781,720,675,1014]	1	hSpCas9	negative selection	7727612
158645737	158645760	3	+	27260156	NCIH2009	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-1.1798749190482614	[627]	[423,521,219]	-8	hSpCas9	negative selection	7848861
158645642	158645665	3	+	27260156	NCIH2009	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.25068134741973647	[845]	[1220,920,882]	-3	hSpCas9	negative selection	7848863
158645737	158645760	3	+	27260156	NCIH1373	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.5868819022291518	[627]	[410,525,423,130]	-5	hSpCas9	negative selection	7970112
158645642	158645665	3	+	27260156	NCIH1373	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.1569164583513466	[845]	[1113,688,514,305]	-1	hSpCas9	negative selection	7970114
158645737	158645760	3	+	27260156	PATU8902	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.9703057668701569	[627]	[645,352,199,670]	-7	hSpCas9	negative selection	8091363
158645642	158645665	3	+	27260156	PATU8902	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.575670554306023	[845]	[1175,676,599,740]	-5	hSpCas9	negative selection	8091365
158645737	158645760	3	+	27260156	PANC1	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.7996518421312875	[627]	[196,331,329,371]	-8	hSpCas9	negative selection	8212614
158645642	158645665	3	+	27260156	PANC1	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.23329833008516632	[845]	[559,729,570,623]	-4	hSpCas9	negative selection	8212616
158645737	158645760	3	+	27260156	PANC0813	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.6786152715486322	[627]	[340,450,403,625]	-7	hSpCas9	negative selection	8333865
158645642	158645665	3	+	27260156	PANC0813	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.07821937257670752	[845]	[1294,1103,955,871]	1	hSpCas9	negative selection	8333867
158645737	158645760	3	+	27260156	RDES	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.1215696129153962	[627]	[487,576,873,767]	-2	hSpCas9	negative selection	8455116
158645642	158645665	3	+	27260156	RDES	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.17205765835088294	[845]	[855,927,1289,1400]	3	hSpCas9	negative selection	8455118
158645737	158645760	3	+	27260156	PC3	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.014495193291119035	[627]	[399,655,1112,529]	0	hSpCas9	negative selection	8576367
158645642	158645665	3	+	27260156	PC3	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.27583747177688867	[845]	[711,798,1868,1076]	6	hSpCas9	negative selection	8576369
158645737	158645760	3	+	27260156	PATU8988T	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.8575546862985615	[627]	[200,352,368,424]	-8	hSpCas9	negative selection	8697618
158645642	158645665	3	+	27260156	PATU8988T	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.01729071851032049	[845]	[708,745,861,915]	0	hSpCas9	negative selection	8697620
158645737	158645760	3	+	27260156	T47D	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.43320953764248255	[627]	[539,634,475,507]	-7	hSpCas9	negative selection	8818869
158645642	158645665	3	+	27260156	T47D	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.007188708486350448	[845]	[1045,942,947,1030]	0	hSpCas9	negative selection	8818871
158645737	158645760	3	+	27260156	SU8686	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.602882766532708	[627]	[398,515,332,366]	-7	hSpCas9	negative selection	8940120
158645642	158645665	3	+	27260156	SU8686	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.1986952354988506	[845]	[1008,869,923,1061]	3	hSpCas9	negative selection	8940122
158645737	158645760	3	+	27260156	SKES1	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.1884597064077759	[627]	[616,615,501,483]	-4	hSpCas9	negative selection	9061371
158645642	158645665	3	+	27260156	SKES1	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.11526708599172697	[845]	[978,1044,799,855]	2	hSpCas9	negative selection	9061373
158645737	158645760	3	+	27260156	TOV112D	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.5970383024141135	[627]	[403,447,382,433]	-8	hSpCas9	negative selection	9182622
158645642	158645665	3	+	27260156	TOV112D	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	-0.03489116122086078	[845]	[844,1040,754,722]	0	hSpCas9	negative selection	9182624
158645737	158645760	3	+	27260156	TC71	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.36789484018496543	[627]	[553,655,400,475]	-7	hSpCas9	negative selection	9303873
158645642	158645665	3	+	27260156	TC71	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.27117283644319035	[845]	[1045,971,1012,1366]	6	hSpCas9	negative selection	9303875
158645737	158645760	3	+	27260156	TC32	viability	GAACGAGTCCTTTACTACACTGG	GFM1	ENSG00000168827	-0.20314990296663304	[627]	[495,323,803,521]	-4	hSpCas9	negative selection	9425124
158645642	158645665	3	+	27260156	TC32	viability	CCTGCCGATGGTCTTCATCAGGG	GFM1	ENSG00000168827	0.09295801188521613	[845]	[849,525,1063,1074]	1	hSpCas9	negative selection	9425126
158644685	158644708	3	-	27869803	HPAFII	viability after 27 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-1.2753461443722798	[202]	[66,24,NaN]	-7	hSpCas9	negative selection	9583309
158645630	158645653	3	+	27869803	HPAFII	viability after 27 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-1.5818918467611893	[43]	[10,4,NaN]	-7	hSpCas9	negative selection	9583310
158644685	158644708	3	-	27869803	HPAFII	viability after 15 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-0.2191072900419314	[202]	[319,104,NaN]	-3	hSpCas9	negative selection	9665758
158645630	158645653	3	+	27869803	HPAFII	viability after 15 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-0.8581704092020312	[43]	[59,4,NaN]	-7	hSpCas9	negative selection	9665759
158644685	158644708	3	-	27869803	ASPC1	viability after 36 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-1.3290826297658564	[450]	[102,89,11]	-8	hSpCas9	negative selection	9748207
158645630	158645653	3	+	27869803	ASPC1	viability after 36 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-0.46535542661147744	[148]	[15,99,2]	-5	hSpCas9	negative selection	9748208
158644685	158644708	3	-	27869803	PATU8988S	viability after 35 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-1.2189148462227197	[516]	[68,109,NaN]	-8	hSpCas9	negative selection	9830656
158645630	158645653	3	+	27869803	PATU8988S	viability after 35 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-4.916775914422066	[74]	[0,0,NaN]	-9	hSpCas9	negative selection	9830657
158644685	158644708	3	-	27869803	PATU8988S	viability after 31 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-1.3321572323679582	[516]	[69,96,NaN]	-8	hSpCas9	negative selection	9913105
158645630	158645653	3	+	27869803	PATU8988S	viability after 31 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-3.2244840720061334	[74]	[0,5,NaN]	-9	hSpCas9	negative selection	9913106
158644685	158644708	3	-	27869803	HPAFII	viability after 35 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-2.3233329050912372	[202]	[28,10,NaN]	-8	hSpCas9	negative selection	9995554
158645630	158645653	3	+	27869803	HPAFII	viability after 35 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-4.477343065640623	[43]	[0,0,NaN]	-9	hSpCas9	negative selection	9995555
158644685	158644708	3	-	27869803	HPAFII	viability after 31 days	TCTGCCAGCCTAGGGAGGCGGGG	GFM1	ENSG00000168827	-2.5423410031969857	[202]	[31,5,NaN]	-8	hSpCas9	negative selection	10078003
158645630	158645653	3	+	27869803	HPAFII	viability after 31 days	TTAATTGGAAGGCCTGCCGATGG	GFM1	ENSG00000168827	-1.8927060783482124	[43]	[9,2,NaN]	-8	hSpCas9	negative selection	10078004
158684550	158684573	3	-	28162770	P31FUJ	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-3.0596533020977796	[148]	[20]	-9	hSpCas9	negative selection	10161700
158690249	158690272	3	+	28162770	P31FUJ	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-1.487616103443204	[209]	[87]	-7	hSpCas9	negative selection	10161701
158684550	158684573	3	-	28162770	TF1	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	NaN	[NaN]	[3]	1	hSpCas9	negative selection	10331066
158690249	158690272	3	+	28162770	TF1	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	NaN	[NaN]	[7]	1	hSpCas9	negative selection	10331067
158684550	158684573	3	-	28162770	SKM1	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-1.348070509689593	[35]	[24]	-7	hSpCas9	negative selection	10500432
158690249	158690272	3	+	28162770	SKM1	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-2.391857306352953	[94]	[31]	-8	hSpCas9	negative selection	10500433
158684550	158684573	3	-	28162770	SKM1	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-1.348070509689593	[35]	[24]	-7	hSpCas9	negative selection	10669798
158690249	158690272	3	+	28162770	SKM1	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-2.391857306352953	[94]	[31]	-8	hSpCas9	negative selection	10669799
158684550	158684573	3	-	28162770	PL21	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	0.07376232871976468	[154]	[70]	0	hSpCas9	negative selection	10839164
158690249	158690272	3	+	28162770	PL21	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-1.1617272257681859	[292]	[56]	-7	hSpCas9	negative selection	10839165
158684550	158684573	3	-	28162770	P31FUJ	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-3.0596533020977796	[148]	[20]	-9	hSpCas9	negative selection	11008530
158690249	158690272	3	+	28162770	P31FUJ	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-1.487616103443204	[209]	[87]	-7	hSpCas9	negative selection	11008531
158684550	158684573	3	-	28162770	OCIAML5	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-0.7216699336083221	[73]	[80]	-5	hSpCas9	negative selection	11177896
158690249	158690272	3	+	28162770	OCIAML5	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-3.2803035678977	[182]	[33]	-9	hSpCas9	negative selection	11177897
158684550	158684573	3	-	28162770	EOL1	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-1.7404140512158415	[240]	[37]	-8	hSpCas9	negative selection	11347262
158690249	158690272	3	+	28162770	EOL1	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-2.9605384537196358	[590]	[39]	-9	hSpCas9	negative selection	11347263
158684550	158684573	3	-	28162770	MOLM13	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-0.6417351096299194	[163]	[75]	-4	hSpCas9	negative selection	11516628
158690249	158690272	3	+	28162770	MOLM13	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-2.7486360327598724	[408]	[43]	-8	hSpCas9	negative selection	11516629
158684550	158684573	3	-	28162770	HEL	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-2.150810290532217	[207]	[29]	-8	hSpCas9	negative selection	11685994
158690249	158690272	3	+	28162770	HEL	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-2.0178879386528714	[410]	[64]	-8	hSpCas9	negative selection	11685995
158684550	158684573	3	-	28162770	MV411	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-2.085545732613583	[130]	[25]	-8	hSpCas9	negative selection	11855360
158690249	158690272	3	+	28162770	MV411	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-3.217230716220669	[275]	[24]	-9	hSpCas9	negative selection	11855361
158684550	158684573	3	-	28162770	MONOMAC1	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	0.06234978175909578	[65]	[78]	0	hSpCas9	negative selection	12024726
158690249	158690272	3	+	28162770	MONOMAC1	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-1.2628389054552998	[156]	[74]	-7	hSpCas9	negative selection	12024727
158684550	158684573	3	-	28162770	OCIAML2	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-0.9852029980719195	[19]	[55]	-6	hSpCas9	negative selection	12194092
158690249	158690272	3	+	28162770	OCIAML2	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-2.7009274446639555	[60]	[51]	-9	hSpCas9	negative selection	12194093
158684550	158684573	3	-	28162770	OCIAML3	viability	AGAGCTTGTGACCAGAAAGAGGG	GFM1	ENSG00000168827	-2.594007640324163	[37]	[18]	-9	hSpCas9	negative selection	12363458
158690249	158690272	3	+	28162770	OCIAML3	viability	GCAGGAATTAACCGACGCCATGG	GFM1	ENSG00000168827	-0.27666681493245265	[62]	[156]	-2	hSpCas9	negative selection	12363459