Crispr

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 10075
• Symbol: HUWE1
• Description: HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1

DataSource: http://genomecrispr.dkfz.de/#!/results/HUWE1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
53604638	53604661	X	+	26472758	Jiyoye	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.4001795405517306	[34]	[9]	-7	hSpCas9	negative selection	75381
53536626	53536649	X	-	26472758	Jiyoye	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-1.9147527133814888	[134]	[26]	-8	hSpCas9	negative selection	75382
53592463	53592486	X	-	26472758	Jiyoye	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.082209459233052	[364]	[64]	-8	hSpCas9	negative selection	75383
53580964	53580987	X	+	26472758	Jiyoye	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	0.3656561120676065	[140]	[136]	2	hSpCas9	negative selection	75384
53569735	53569758	X	+	26472758	Jiyoye	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.0937228544268083	[149]	[52]	-6	hSpCas9	negative selection	75385
53562140	53562163	X	-	26472758	Jiyoye	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-0.48838720830113225	[332]	[178]	-4	hSpCas9	negative selection	75386
53550911	53550934	X	+	26472758	Jiyoye	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-1.5035572803970392	[93]	[24]	-7	hSpCas9	negative selection	75387
53547728	53547751	X	+	26472758	Jiyoye	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-2.123339335192906	[51]	[8]	-8	hSpCas9	negative selection	75388
53546787	53546810	X	-	26472758	Jiyoye	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-2.1484715523907267	[340]	[57]	-8	hSpCas9	negative selection	75389
53546519	53546542	X	-	26472758	Jiyoye	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-2.177787119215283	[17]	[2]	-8	hSpCas9	negative selection	75390
53604638	53604661	X	+	26472758	KBM7	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-0.6542604418316857	[176,302]	[64,81]	-5	hSpCas9	negative selection	266499
53536626	53536649	X	-	26472758	KBM7	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-0.9497025388859233	[476,505]	[99,138]	-6	hSpCas9	negative selection	266500
53592463	53592486	X	-	26472758	KBM7	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-1.39751001683227	[1113,823]	[177,164]	-7	hSpCas9	negative selection	266501
53580964	53580987	X	+	26472758	KBM7	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	0.32868682121403847	[745,559]	[412,353]	3	hSpCas9	negative selection	266502
53569735	53569758	X	+	26472758	KBM7	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-0.7733896827561411	[1065,740]	[156,323]	-6	hSpCas9	negative selection	266503
53562140	53562163	X	-	26472758	KBM7	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	0.10268358087718032	[931,781]	[460,403]	1	hSpCas9	negative selection	266504
53550911	53550934	X	+	26472758	KBM7	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-2.0994921830289295	[560,369]	[54,45]	-8	hSpCas9	negative selection	266505
53547728	53547751	X	+	26472758	KBM7	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-0.17156217493173975	[185,165]	[27,113]	-2	hSpCas9	negative selection	266506
53546787	53546810	X	-	26472758	KBM7	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.2767765499213781	[981,596]	[175,126]	-7	hSpCas9	negative selection	266507
53546519	53546542	X	-	26472758	KBM7	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-3.2495890981157722	[68,93]	[1,5]	-9	hSpCas9	negative selection	266508
53604638	53604661	X	+	26472758	Raji	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-2.865309118630665	[156]	[4]	-9	hSpCas9	negative selection	457617
53536626	53536649	X	-	26472758	Raji	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-2.1564416049501944	[364]	[18]	-8	hSpCas9	negative selection	457618
53592463	53592486	X	-	26472758	Raji	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-1.1117849850885622	[446]	[47]	-7	hSpCas9	negative selection	457619
53580964	53580987	X	+	26472758	Raji	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-1.1189668995315318	[364]	[38]	-7	hSpCas9	negative selection	457620
53569735	53569758	X	+	26472758	Raji	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-0.7493835808090825	[296]	[40]	-5	hSpCas9	negative selection	457621
53562140	53562163	X	-	26472758	Raji	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	0.3877715079576615	[302]	[91]	3	hSpCas9	negative selection	457622
53550911	53550934	X	+	26472758	Raji	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-1.7576868845402922	[203]	[13]	-8	hSpCas9	negative selection	457623
53547728	53547751	X	+	26472758	Raji	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	0.5025214772147384	[72]	[23]	5	hSpCas9	negative selection	457624
53546787	53546810	X	-	26472758	Raji	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.3197877195410994	[397]	[36]	-7	hSpCas9	negative selection	457625
53546519	53546542	X	-	26472758	Raji	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-1.565041806597896	[50]	[3]	-8	hSpCas9	negative selection	457626
53604638	53604661	X	+	26472758	K562	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-0.17256704340191875	[246]	[184]	-1	hSpCas9	negative selection	648735
53536626	53536649	X	-	26472758	K562	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-3.217582250582984	[561]	[50]	-8	hSpCas9	negative selection	648736
53592463	53592486	X	-	26472758	K562	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.100873360286426	[746]	[146]	-7	hSpCas9	negative selection	648737
53580964	53580987	X	+	26472758	K562	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	0.6494532587600128	[397]	[526]	5	hSpCas9	negative selection	648738
53569735	53569758	X	+	26472758	K562	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.3041149860999837	[742]	[253]	-6	hSpCas9	negative selection	648739
53562140	53562163	X	-	26472758	K562	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-0.05299308761388433	[670]	[545]	0	hSpCas9	negative selection	648740
53550911	53550934	X	+	26472758	K562	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-0.856011363173431	[460]	[214]	-5	hSpCas9	negative selection	648741
53547728	53547751	X	+	26472758	K562	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-0.32981736646165083	[133]	[89]	-2	hSpCas9	negative selection	648742
53546787	53546810	X	-	26472758	K562	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.1979692597573526	[567]	[208]	-6	hSpCas9	negative selection	648743
53546519	53546542	X	-	26472758	K562	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-5.3405437730547085	[47]	[0]	-9	hSpCas9	negative selection	648744
53627795	53627818	X	-	26627737	DLD1	viability	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.36390211978000586	[159]	[105,48,40]	4	hSpCas9	negative selection	846045
53628540	53628563	X	+	26627737	DLD1	viability	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-1.3940972182331932	[522]	[89,71,29]	-8	hSpCas9	negative selection	846046
53628796	53628819	X	-	26627737	DLD1	viability	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-2.9673465715068366	[173]	[2,4,11]	-9	hSpCas9	negative selection	846047
53631413	53631436	X	-	26627737	DLD1	viability	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.6703425295336147	[1000]	[149,285,162]	-6	hSpCas9	negative selection	846048
53645323	53645346	X	+	26627737	DLD1	viability	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.8487356534586477	[1377]	[89,201,76]	-8	hSpCas9	negative selection	846049
53647520	53647543	X	+	26627737	DLD1	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.6186095071364908	[1763]	[182,73,263]	-8	hSpCas9	negative selection	846050
53627795	53627818	X	-	26627737	GBM cells	viability after 5 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.6813224398130588	[99]	[81,89]	8	hSpCas9	negative selection	928360
53628540	53628563	X	+	26627737	GBM cells	viability after 5 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	0.09242155078555142	[208]	[119,118]	1	hSpCas9	negative selection	928361
53628796	53628819	X	-	26627737	GBM cells	viability after 5 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.5104770612520162	[84]	[8,22]	-9	hSpCas9	negative selection	928362
53631413	53631436	X	-	26627737	GBM cells	viability after 5 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.194195529505796	[607]	[286,282]	-3	hSpCas9	negative selection	928363
53645323	53645346	X	+	26627737	GBM cells	viability after 5 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.771484102341531	[599]	[181,194]	-8	hSpCas9	negative selection	928364
53647520	53647543	X	+	26627737	GBM cells	viability after 5 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.2174508818060987	[1083]	[103,145]	-9	hSpCas9	negative selection	928365
53627795	53627818	X	-	26627737	GBM cells	viability after 13 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.7279768582404276	[99]	[101,79]	8	hSpCas9	negative selection	1010675
53628540	53628563	X	+	26627737	GBM cells	viability after 13 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-0.5799829485048278	[208]	[71,78]	-6	hSpCas9	negative selection	1010676
53628796	53628819	X	-	26627737	GBM cells	viability after 13 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-3.0911886977525804	[84]	[0,8]	-9	hSpCas9	negative selection	1010677
53631413	53631436	X	-	26627737	GBM cells	viability after 13 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.6291737566136201	[607]	[236,193]	-6	hSpCas9	negative selection	1010678
53645323	53645346	X	+	26627737	GBM cells	viability after 13 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.2009332336662	[599]	[118,159]	-8	hSpCas9	negative selection	1010679
53647520	53647543	X	+	26627737	GBM cells	viability after 13 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-3.131425682737082	[1083]	[97,41]	-9	hSpCas9	negative selection	1010680
53627795	53627818	X	-	26627737	GBM cells	viability after 21 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.31627665701480456	[99]	[75,59]	3	hSpCas9	negative selection	1092990
53628540	53628563	X	+	26627737	GBM cells	viability after 21 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-1.1208408763937834	[208]	[58,45]	-7	hSpCas9	negative selection	1092991
53628796	53628819	X	-	26627737	GBM cells	viability after 21 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-5.529157636522044	[84]	[0,0]	-9	hSpCas9	negative selection	1092992
53631413	53631436	X	-	26627737	GBM cells	viability after 21 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-1.6869635388484527	[607]	[123,82]	-8	hSpCas9	negative selection	1092993
53645323	53645346	X	+	26627737	GBM cells	viability after 21 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.4729554798638499	[599]	[249,220]	-4	hSpCas9	negative selection	1092994
53647520	53647543	X	+	26627737	GBM cells	viability after 21 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-3.205677660534412	[1083]	[55,70]	-9	hSpCas9	negative selection	1092995
53627795	53627818	X	-	26627737	RPE1	viability after 9 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-0.3916827060724979	[385]	[85,99]	-3	hSpCas9	negative selection	1175305
53628540	53628563	X	+	26627737	RPE1	viability after 9 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-1.8258634895980927	[892]	[39,128]	-8	hSpCas9	negative selection	1175306
53628796	53628819	X	-	26627737	RPE1	viability after 9 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-4.610184017271636	[622]	[0,16]	-9	hSpCas9	negative selection	1175307
53631413	53631436	X	-	26627737	RPE1	viability after 9 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-1.55732117595776	[1329]	[99,194]	-8	hSpCas9	negative selection	1175308
53645323	53645346	X	+	26627737	RPE1	viability after 9 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-2.009228845926284	[1113]	[90,80]	-8	hSpCas9	negative selection	1175309
53647520	53647543	X	+	26627737	RPE1	viability after 9 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.9641487684676164	[3218]	[74,198]	-9	hSpCas9	negative selection	1175310
53627795	53627818	X	-	26627737	RPE1	viability after 12 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-3.5541656146355347	[385]	[2,14]	-9	hSpCas9	negative selection	1257620
53628540	53628563	X	+	26627737	RPE1	viability after 12 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-2.710382515303369	[892]	[24,48]	-9	hSpCas9	negative selection	1257621
53628796	53628819	X	-	26627737	RPE1	viability after 12 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-5.418227875335304	[622]	[0,6]	-9	hSpCas9	negative selection	1257622
53631413	53631436	X	-	26627737	RPE1	viability after 12 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-1.289299617650339	[1329]	[118,174]	-7	hSpCas9	negative selection	1257623
53645323	53645346	X	+	26627737	RPE1	viability after 12 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.9693819464359887	[1113]	[56,96]	-8	hSpCas9	negative selection	1257624
53647520	53647543	X	+	26627737	RPE1	viability after 12 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-3.4230661941522422	[3218]	[33,129]	-9	hSpCas9	negative selection	1257625
53627795	53627818	X	-	26627737	RPE1	viability after 15 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-2.579185527456144	[385]	[2,32]	-8	hSpCas9	negative selection	1339935
53628540	53628563	X	+	26627737	RPE1	viability after 15 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-4.313085963062149	[892]	[0,23]	-9	hSpCas9	negative selection	1339936
53628796	53628819	X	-	26627737	RPE1	viability after 15 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-7.461339479152108	[622]	[0,0]	-9	hSpCas9	negative selection	1339937
53631413	53631436	X	-	26627737	RPE1	viability after 15 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-2.504357315568652	[1329]	[48,82]	-8	hSpCas9	negative selection	1339938
53645323	53645346	X	+	26627737	RPE1	viability after 15 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-2.3346794339143226	[1113]	[35,87]	-8	hSpCas9	negative selection	1339939
53647520	53647543	X	+	26627737	RPE1	viability after 15 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.902668970790541	[3218]	[162,81]	-9	hSpCas9	negative selection	1339940
53627795	53627818	X	-	26627737	RPE1	viability after 18 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-1.8191031087881027	[385]	[0,59]	-8	hSpCas9	negative selection	1422250
53628540	53628563	X	+	26627737	RPE1	viability after 18 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-7.312019198840303	[892]	[1,0]	-9	hSpCas9	negative selection	1422251
53628796	53628819	X	-	26627737	RPE1	viability after 18 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-7.393420092923259	[622]	[0,0]	-9	hSpCas9	negative selection	1422252
53631413	53631436	X	-	26627737	RPE1	viability after 18 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-1.317759722071077	[1329]	[128,159]	-7	hSpCas9	negative selection	1422253
53645323	53645346	X	+	26627737	RPE1	viability after 18 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-2.223131307098111	[1113]	[31,98]	-8	hSpCas9	negative selection	1422254
53647520	53647543	X	+	26627737	RPE1	viability after 18 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-3.591759372519416	[3218]	[58,85]	-9	hSpCas9	negative selection	1422255
53627795	53627818	X	-	26627737	HeLa	viability after 8 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	1.1100462831260232	[115]	[72,122,121]	8	hSpCas9	negative selection	1504565
53628540	53628563	X	+	26627737	HeLa	viability after 8 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	2.043162035990731	[119]	[184,90,352]	9	hSpCas9	negative selection	1504566
53628796	53628819	X	-	26627737	HeLa	viability after 8 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-2.3701508373118734	[245]	[53,2,12]	-9	hSpCas9	negative selection	1504567
53631413	53631436	X	-	26627737	HeLa	viability after 8 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.9337532799391123	[1372]	[528,160,296]	-7	hSpCas9	negative selection	1504568
53645323	53645346	X	+	26627737	HeLa	viability after 8 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	0.1309284748560653	[1040]	[440,643,397]	1	hSpCas9	negative selection	1504569
53647520	53647543	X	+	26627737	HeLa	viability after 8 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.6215073186671711	[2301]	[284,494,196]	-9	hSpCas9	negative selection	1504570
53627795	53627818	X	-	26627737	HeLa	viability after 12 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	1.4104289912307248	[115]	[107,171,96]	8	hSpCas9	negative selection	1586880
53628540	53628563	X	+	26627737	HeLa	viability after 12 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	1.559823883445983	[119]	[164,109,167]	9	hSpCas9	negative selection	1586881
53628796	53628819	X	-	26627737	HeLa	viability after 12 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-6.261660271551277	[245]	[0,0,1]	-9	hSpCas9	negative selection	1586882
53631413	53631436	X	-	26627737	HeLa	viability after 12 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.4093888899662974	[1372]	[352,469,451]	-4	hSpCas9	negative selection	1586883
53645323	53645346	X	+	26627737	HeLa	viability after 12 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.016346950532247106	[1040]	[582,308,400]	0	hSpCas9	negative selection	1586884
53647520	53647543	X	+	26627737	HeLa	viability after 12 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.2157630130130523	[2301]	[156,251,198]	-9	hSpCas9	negative selection	1586885
53627795	53627818	X	-	26627737	HeLa	viability after 15 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.9316704803382787	[115]	[55,168,41]	7	hSpCas9	negative selection	1669195
53628540	53628563	X	+	26627737	HeLa	viability after 15 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	0.6872487974808783	[119]	[19,134,81]	5	hSpCas9	negative selection	1669196
53628796	53628819	X	-	26627737	HeLa	viability after 15 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-6.645981393031234	[245]	[0,0,0]	-9	hSpCas9	negative selection	1669197
53631413	53631436	X	-	26627737	HeLa	viability after 15 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.3073258856445624	[1372]	[445,459,448]	-3	hSpCas9	negative selection	1669198
53645323	53645346	X	+	26627737	HeLa	viability after 15 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.7252051497014422	[1040]	[269,192,309]	-6	hSpCas9	negative selection	1669199
53647520	53647543	X	+	26627737	HeLa	viability after 15 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.504617763241589	[2301]	[58,269,167]	-9	hSpCas9	negative selection	1669200
53627795	53627818	X	-	26627737	HeLa	viability after 18 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.9107437374830508	[115]	[34,139,41]	7	hSpCas9	negative selection	1751510
53628540	53628563	X	+	26627737	HeLa	viability after 18 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	0.7203742197307735	[119]	[15,112,81]	5	hSpCas9	negative selection	1751511
53628796	53628819	X	-	26627737	HeLa	viability after 18 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-6.335775741377367	[245]	[0,0,0]	-9	hSpCas9	negative selection	1751512
53631413	53631436	X	-	26627737	HeLa	viability after 18 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.30235113562533344	[1372]	[290,394,448]	-3	hSpCas9	negative selection	1751513
53645323	53645346	X	+	26627737	HeLa	viability after 18 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.7356828896486745	[1040]	[174,162,309]	-6	hSpCas9	negative selection	1751514
53647520	53647543	X	+	26627737	HeLa	viability after 18 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.5021074927111977	[2301]	[42,219,167]	-9	hSpCas9	negative selection	1751515
53627795	53627818	X	-	26627737	HCT116	viability after 6 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.22622905859172693	[267]	[76,33]	1	hSpCas9	negative selection	1833825
53628540	53628563	X	+	26627737	HCT116	viability after 6 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-1.4208843136998097	[637]	[79,13]	-7	hSpCas9	negative selection	1833826
53628796	53628819	X	-	26627737	HCT116	viability after 6 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	1.352514216728586	[161]	[133,26]	7	hSpCas9	negative selection	1833827
53631413	53631436	X	-	26627737	HCT116	viability after 6 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	1.216358864694372	[835]	[432,234]	6	hSpCas9	negative selection	1833828
53645323	53645346	X	+	26627737	HCT116	viability after 6 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	1.3396489906665632	[931]	[792,137]	7	hSpCas9	negative selection	1833829
53647520	53647543	X	+	26627737	HCT116	viability after 6 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.14519852481074458	[2498]	[614,211]	-1	hSpCas9	negative selection	1833830
53627795	53627818	X	-	26627737	HCT116	viability after 8 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.3660110120529434	[127]	[113,58,99]	4	hSpCas9	negative selection	1916140
53628540	53628563	X	+	26627737	HCT116	viability after 8 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-0.7654206720255472	[286]	[141,46,90]	-7	hSpCas9	negative selection	1916141
53628796	53628819	X	-	26627737	HCT116	viability after 8 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-2.8822773875570684	[83]	[11,5,0]	-9	hSpCas9	negative selection	1916142
53631413	53631436	X	-	26627737	HCT116	viability after 8 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	0.024373324215347214	[862]	[429,539,479]	0	hSpCas9	negative selection	1916143
53645323	53645346	X	+	26627737	HCT116	viability after 8 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.8508271423687561	[458]	[154,127,137]	-8	hSpCas9	negative selection	1916144
53647520	53647543	X	+	26627737	HCT116	viability after 8 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.123736807184665	[1259]	[345,346,263]	-8	hSpCas9	negative selection	1916145
53627795	53627818	X	-	26627737	HCT116	viability after 9 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-0.42999443635934	[267]	[113,4]	-3	hSpCas9	negative selection	1998455
53628540	53628563	X	+	26627737	HCT116	viability after 9 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-0.5749795414787271	[637]	[253,3]	-4	hSpCas9	negative selection	1998456
53628796	53628819	X	-	26627737	HCT116	viability after 9 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.7155484619288248	[161]	[1,21]	-8	hSpCas9	negative selection	1998457
53631413	53631436	X	-	26627737	HCT116	viability after 9 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	0.8305276570574442	[835]	[525,294]	6	hSpCas9	negative selection	1998458
53645323	53645346	X	+	26627737	HCT116	viability after 9 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	0.5309589008038831	[931]	[334,378]	4	hSpCas9	negative selection	1998459
53647520	53647543	X	+	26627737	HCT116	viability after 9 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.1343461293529382	[2498]	[308,299]	-7	hSpCas9	negative selection	1998460
53627795	53627818	X	-	26627737	HCT116	viability after 12 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.2558432977497732	[127]	[153,18,59]	2	hSpCas9	negative selection	2080770
53628540	53628563	X	+	26627737	HCT116	viability after 12 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-0.7277918843052349	[286]	[112,78,78]	-7	hSpCas9	negative selection	2080771
53628796	53628819	X	-	26627737	HCT116	viability after 12 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.3561682036511742	[83]	[0,51,0]	-8	hSpCas9	negative selection	2080772
53631413	53631436	X	-	26627737	HCT116	viability after 12 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.5018950876341473	[862]	[349,352,255]	-5	hSpCas9	negative selection	2080773
53645323	53645346	X	+	26627737	HCT116	viability after 12 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.864499348358485	[458]	[176,109,105]	-7	hSpCas9	negative selection	2080774
53647520	53647543	X	+	26627737	HCT116	viability after 12 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.069221809041785	[1259]	[144,137,190]	-9	hSpCas9	negative selection	2080775
53627795	53627818	X	-	26627737	HCT116	viability after 15 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-0.13222847963338258	[127]	[67,38,93]	-1	hSpCas9	negative selection	2163085
53628540	53628563	X	+	26627737	HCT116	viability after 15 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-0.9315158045250341	[286]	[132,51,79]	-7	hSpCas9	negative selection	2163086
53628796	53628819	X	-	26627737	HCT116	viability after 15 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-4.259158713718579	[83]	[5,0,0]	-9	hSpCas9	negative selection	2163087
53631413	53631436	X	-	26627737	HCT116	viability after 15 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.8619324016797905	[862]	[312,237,271]	-7	hSpCas9	negative selection	2163088
53645323	53645346	X	+	26627737	HCT116	viability after 15 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.9024657465408896	[458]	[166,99,158]	-7	hSpCas9	negative selection	2163089
53647520	53647543	X	+	26627737	HCT116	viability after 15 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.529436701365735	[1259]	[85,141,142]	-9	hSpCas9	negative selection	2163090
53627795	53627818	X	-	26627737	HCT116	viability after 18 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.4653487862171105	[127]	[152,8,99]	4	hSpCas9	negative selection	2245400
53628540	53628563	X	+	26627737	HCT116	viability after 18 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-0.46596190925678355	[286]	[90,115,92]	-4	hSpCas9	negative selection	2245401
53628796	53628819	X	-	26627737	HCT116	viability after 18 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-4.5733412040423165	[83]	[0,2,0]	-9	hSpCas9	negative selection	2245402
53631413	53631436	X	-	26627737	HCT116	viability after 18 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.7259143638245846	[862]	[264,304,179]	-6	hSpCas9	negative selection	2245403
53645323	53645346	X	+	26627737	HCT116	viability after 18 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.71129297610006	[458]	[116,32,54]	-8	hSpCas9	negative selection	2245404
53647520	53647543	X	+	26627737	HCT116	viability after 18 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.369740917941566	[1259]	[106,92,156]	-9	hSpCas9	negative selection	2245405
53647513	53647536	X	-	24336569	HL60	viability	GATGGAATACTGGCAGATGCTGG	HUWE1	ENSG00000086758	-2.2939137187177656	[481]	[207]	-9	hSpCas9	negative selection	2271080
53647520	53647543	X	+	24336569	HL60	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.7972556716906922	[123]	[150]	-6	hSpCas9	negative selection	2271081
53647529	53647552	X	+	24336569	HL60	viability	TTCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	0.36437409814530086	[474]	[1293]	3	hSpCas9	negative selection	2271082
53647544	53647567	X	-	24336569	HL60	viability	ATATCACTGGGTGGACCTGTTGG	HUWE1	ENSG00000086758	-1.3421920390795212	[749]	[625]	-8	hSpCas9	negative selection	2271083
53647553	53647576	X	-	24336569	HL60	viability	GTGCGAGTTATATCACTGGGTGG	HUWE1	ENSG00000086758	-0.1763232869703375	[384]	[720]	-1	hSpCas9	negative selection	2271084
53648247	53648270	X	-	24336569	HL60	viability	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	1.0156103354057346	[175]	[752]	7	hSpCas9	negative selection	2271085
53648272	53648295	X	+	24336569	HL60	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	2.5140676418024475	[67]	[821]	9	hSpCas9	negative selection	2271086
53648288	53648311	X	+	24336569	HL60	viability	ATTAAGGCTCTGCAGTCTGCAGG	HUWE1	ENSG00000086758	-0.2518792311878264	[183]	[326]	-2	hSpCas9	negative selection	2271087
53654065	53654088	X	-	24336569	HL60	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.8308134192509351	[389]	[463]	-6	hSpCas9	negative selection	2271088
53654093	53654116	X	-	24336569	HL60	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.5162648547291702	[587]	[869]	-5	hSpCas9	negative selection	2271089
53647513	53647536	X	-	24336569	KBM7	viability	GATGGAATACTGGCAGATGCTGG	HUWE1	ENSG00000086758	-2.0747993847317456	[484]	[313]	-8	hSpCas9	negative selection	2338257
53647520	53647543	X	+	24336569	KBM7	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.7192525206565492	[133]	[221]	-5	hSpCas9	negative selection	2338258
53647529	53647552	X	+	24336569	KBM7	viability	TTCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	1.4580022383156364	[293]	[2202]	9	hSpCas9	negative selection	2338259
53647544	53647567	X	-	24336569	KBM7	viability	ATATCACTGGGTGGACCTGTTGG	HUWE1	ENSG00000086758	-0.405350961159585	[504]	[1039]	-3	hSpCas9	negative selection	2338260
53647553	53647576	X	-	24336569	KBM7	viability	GTGCGAGTTATATCACTGGGTGG	HUWE1	ENSG00000086758	-2.7008945976472245	[502]	[210]	-9	hSpCas9	negative selection	2338261
53648247	53648270	X	-	24336569	KBM7	viability	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-0.5275692947225564	[342]	[648]	-4	hSpCas9	negative selection	2338262
53648272	53648295	X	+	24336569	KBM7	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-1.2345854732146846	[131]	[152]	-7	hSpCas9	negative selection	2338263
53648288	53648311	X	+	24336569	KBM7	viability	ATTAAGGCTCTGCAGTCTGCAGG	HUWE1	ENSG00000086758	-2.3586697806866868	[644]	[342]	-8	hSpCas9	negative selection	2338264
53654065	53654088	X	-	24336569	KBM7	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	0.5180965427102501	[318]	[1245]	5	hSpCas9	negative selection	2338265
53654093	53654116	X	-	24336569	KBM7	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.7834813254715107	[803]	[1273]	-6	hSpCas9	negative selection	2338266
53625857	53625880	X	+	25494202	A375	resistance to PLX-4720 (puromycin)	CCGGGGCCGGGGCCGGGGCCGGG	HUWE1	ENSG00000086758	0.8264823941872752	[4,0]	[0,0]	3	dCas9-VP64	positive selection	2401664
53625863	53625886	X	+	25494202	A375	resistance to PLX-4720 (puromycin)	CCGGGGCCGGGGCCGGGGCCAGG	HUWE1	ENSG00000086758	0.8264823941872752	[4,0]	[0,0]	3	dCas9-VP64	positive selection	2401676
53625833	53625856	X	+	25494202	A375	resistance to PLX-4720 (puromycin)	CCGGGGCCGGGGCCGGGGCCAGG	HUWE1	ENSG00000086758	0.8264823941872752	[4,0]	[0,0]	3	dCas9-VP64	positive selection	2401677
53566565	53566588	X	+	25494202	A375	resistance to PLX-4720 (puromycin)	TCCCACCTCAGCCTCCCAAAAAG	HUWE1	ENSG00000086758	-0.684504052123245	[138,151]	[23,13]	-3	dCas9-VP64	positive selection	2425536
53553422	53553445	X	+	25494202	A375	resistance to PLX-4720 (puromycin)	GTGAGCCACCGCGCCCAGCCCGG	HUWE1	ENSG00000086758	-0.6476236116882323	[118,215]	[30,13]	-3	dCas9-VP64	positive selection	2438085
53577821	53577844	X	+	25494202	A375	resistance to PLX-4720 (puromycin)	GTGCTCAATGGTGCCCAGGCTGG	HUWE1	ENSG00000086758	-0.5084711381371269	[26,78]	[5,9]	-2	dCas9-VP64	positive selection	2462545
53555602	53555625	X	+	25494202	A375	resistance to PLX-4720 (puromycin)	CAGGCGTGAGCCACTGCGTCCAG	HUWE1	ENSG00000086758	-1.453588368366345	[188,63]	[8,9]	-8	dCas9-VP64	positive selection	2463506
53625857	53625880	X	+	25494202	A375	resistance to PLX-4720 (zeocin)	CCGGGGCCGGGGCCGGGGCCGGG	HUWE1	ENSG00000086758	-1.4525966344186143	[120,27]	[8,7]	-9	dCas9-VP64	positive selection	2496919
53625863	53625886	X	+	25494202	A375	resistance to PLX-4720 (zeocin)	CCGGGGCCGGGGCCGGGGCCAGG	HUWE1	ENSG00000086758	-1.4525966344186143	[120,27]	[8,7]	-9	dCas9-VP64	positive selection	2496931
53625833	53625856	X	+	25494202	A375	resistance to PLX-4720 (zeocin)	CCGGGGCCGGGGCCGGGGCCAGG	HUWE1	ENSG00000086758	-1.4525966344186143	[120,27]	[8,7]	-9	dCas9-VP64	positive selection	2496932
53566565	53566588	X	+	25494202	A375	resistance to PLX-4720 (zeocin)	TCCCACCTCAGCCTCCCAAAAAG	HUWE1	ENSG00000086758	0.29312304600633954	[90,145]	[39,52]	2	dCas9-VP64	positive selection	2520791
53553422	53553445	X	+	25494202	A375	resistance to PLX-4720 (zeocin)	GTGAGCCACCGCGCCCAGCCCGG	HUWE1	ENSG00000086758	-0.5349507089340686	[327,308]	[84,58]	-5	dCas9-VP64	positive selection	2533340
53577821	53577844	X	+	25494202	A375	resistance to PLX-4720 (zeocin)	GTGCTCAATGGTGCCCAGGCTGG	HUWE1	ENSG00000086758	-0.1772018497958423	[109,268]	[34,71]	-2	dCas9-VP64	positive selection	2557800
53555602	53555625	X	+	25494202	A375	resistance to PLX-4720 (zeocin)	CAGGCGTGAGCCACTGCGTCCAG	HUWE1	ENSG00000086758	0.10976477844408938	[73,10]	[24,4]	0	dCas9-VP64	positive selection	2558761
53647556	53647579	X	-	27260157	DLD1	viability	CCAGTGCGAGTTATATCACTGGG	HUWE1	ENSG00000086758	-1.96443736699518	[]	[]	-8	hSpCas9	negative selection	2573764
53648247	53648270	X	-	27260157	DLD1	viability	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-1.87491123950064	[]	[]	-8	hSpCas9	negative selection	2574035
53645316	53645339	X	-	27260157	DLD1	viability	GCTAACTCGGCTACAACATTTGG	HUWE1	ENSG00000086758	-1.28141663575525	[]	[]	-7	hSpCas9	negative selection	2581700
53647529	53647552	X	+	27260157	DLD1	viability	TTCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-0.628471474887133	[]	[]	-5	hSpCas9	negative selection	2581799
53628796	53628819	X	-	27260157	DLD1	viability	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-0.581261904067373	[]	[]	-5	hSpCas9	negative selection	2586419
53629521	53629544	X	+	27260157	DLD1	viability	GCTGCTTATCCGTTATCTGAAGG	HUWE1	ENSG00000086758	-1.03896967990838	[]	[]	-7	hSpCas9	negative selection	2586941
53647513	53647536	X	-	27260157	DLD1	viability	GATGGAATACTGGCAGATGCTGG	HUWE1	ENSG00000086758	-1.93844540700282	[]	[]	-8	hSpCas9	negative selection	2605793
53647523	53647546	X	-	27260157	DLD1	viability	GGACCGCTTCGATGGAATACTGG	HUWE1	ENSG00000086758	-1.4167270368813	[]	[]	-8	hSpCas9	negative selection	2606981
53629596	53629619	X	-	27260157	DLD1	viability	AGTGTATTCCAATGCCTTGCAGG	HUWE1	ENSG00000086758	-2.30768450532277	[]	[]	-9	hSpCas9	negative selection	2607419
53647553	53647576	X	-	27260157	DLD1	viability	GTGCGAGTTATATCACTGGGTGG	HUWE1	ENSG00000086758	-2.01371929692206	[]	[]	-8	hSpCas9	negative selection	2607943
53648288	53648311	X	+	27260157	DLD1	viability	ATTAAGGCTCTGCAGTCTGCAGG	HUWE1	ENSG00000086758	-1.37546297857725	[]	[]	-8	hSpCas9	negative selection	2610405
53647520	53647543	X	+	27260157	DLD1	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.22205516661397	[]	[]	-7	hSpCas9	negative selection	2614191
53648272	53648295	X	+	27260157	DLD1	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.411627107218165	[]	[]	4	hSpCas9	negative selection	2618121
53632536	53632559	X	+	27260157	DLD1	viability	TGTAGTGTAGTTGCACTGGGTGG	HUWE1	ENSG00000086758	-1.35118673781715	[]	[]	-8	hSpCas9	negative selection	2618985
53654093	53654116	X	-	27260157	DLD1	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.0189500939001901	[]	[]	0	hSpCas9	negative selection	2620254
53628609	53628632	X	-	27260157	DLD1	viability	CTCCCTTGTAGATCCTTCCATGG	HUWE1	ENSG00000086758	-2.23181532356558	[]	[]	-9	hSpCas9	negative selection	2621016
53647470	53647493	X	-	27260157	DLD1	viability	CATGGATGCTCGTATGTGATAGG	HUWE1	ENSG00000086758	-1.37098169993161	[]	[]	-8	hSpCas9	negative selection	2623898
53647544	53647567	X	-	27260157	DLD1	viability	ATATCACTGGGTGGACCTGTTGG	HUWE1	ENSG00000086758	-2.11967067792372	[]	[]	-9	hSpCas9	negative selection	2623923
53630962	53630985	X	-	27260157	DLD1	viability	GAAGCGATTGCAGGCAGTTCAGG	HUWE1	ENSG00000086758	-1.7713901696089	[]	[]	-8	hSpCas9	negative selection	2642460
53647556	53647579	X	-	27260157	HT1080	viability	CCAGTGCGAGTTATATCACTGGG	HUWE1	ENSG00000086758	-2.49325065709827	[]	[]	-9	hSpCas9	negative selection	2649894
53648247	53648270	X	-	27260157	HT1080	viability	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-1.66748792877297	[]	[]	-9	hSpCas9	negative selection	2650165
53645316	53645339	X	-	27260157	HT1080	viability	GCTAACTCGGCTACAACATTTGG	HUWE1	ENSG00000086758	-0.757445055070769	[]	[]	-8	hSpCas9	negative selection	2657830
53647529	53647552	X	+	27260157	HT1080	viability	TTCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-1.79922524408168	[]	[]	-9	hSpCas9	negative selection	2657929
53628796	53628819	X	-	27260157	HT1080	viability	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.25312146532226	[]	[]	-9	hSpCas9	negative selection	2662549
53629521	53629544	X	+	27260157	HT1080	viability	GCTGCTTATCCGTTATCTGAAGG	HUWE1	ENSG00000086758	-2.07448513131004	[]	[]	-9	hSpCas9	negative selection	2663071
53647513	53647536	X	-	27260157	HT1080	viability	GATGGAATACTGGCAGATGCTGG	HUWE1	ENSG00000086758	-2.52333564834707	[]	[]	-9	hSpCas9	negative selection	2681923
53647523	53647546	X	-	27260157	HT1080	viability	GGACCGCTTCGATGGAATACTGG	HUWE1	ENSG00000086758	-2.70844281608206	[]	[]	-9	hSpCas9	negative selection	2683111
53629596	53629619	X	-	27260157	HT1080	viability	AGTGTATTCCAATGCCTTGCAGG	HUWE1	ENSG00000086758	-2.89552087165596	[]	[]	-9	hSpCas9	negative selection	2683549
53647553	53647576	X	-	27260157	HT1080	viability	GTGCGAGTTATATCACTGGGTGG	HUWE1	ENSG00000086758	-3.39428627444774	[]	[]	-9	hSpCas9	negative selection	2684073
53648288	53648311	X	+	27260157	HT1080	viability	ATTAAGGCTCTGCAGTCTGCAGG	HUWE1	ENSG00000086758	-2.11416243618689	[]	[]	-9	hSpCas9	negative selection	2686535
53647520	53647543	X	+	27260157	HT1080	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-3.38904799192677	[]	[]	-9	hSpCas9	negative selection	2690321
53648272	53648295	X	+	27260157	HT1080	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.398736690838403	[]	[]	3	hSpCas9	negative selection	2694251
53632536	53632559	X	+	27260157	HT1080	viability	TGTAGTGTAGTTGCACTGGGTGG	HUWE1	ENSG00000086758	-0.769366497838197	[]	[]	-8	hSpCas9	negative selection	2695115
53654093	53654116	X	-	27260157	HT1080	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.0388020105961386	[]	[]	0	hSpCas9	negative selection	2696384
53628609	53628632	X	-	27260157	HT1080	viability	CTCCCTTGTAGATCCTTCCATGG	HUWE1	ENSG00000086758	-1.74350531601511	[]	[]	-9	hSpCas9	negative selection	2697146
53647470	53647493	X	-	27260157	HT1080	viability	CATGGATGCTCGTATGTGATAGG	HUWE1	ENSG00000086758	-2.75768250980026	[]	[]	-9	hSpCas9	negative selection	2700028
53647544	53647567	X	-	27260157	HT1080	viability	ATATCACTGGGTGGACCTGTTGG	HUWE1	ENSG00000086758	-1.38958310017045	[]	[]	-9	hSpCas9	negative selection	2700053
53630962	53630985	X	-	27260157	HT1080	viability	GAAGCGATTGCAGGCAGTTCAGG	HUWE1	ENSG00000086758	-1.28696706039286	[]	[]	-9	hSpCas9	negative selection	2718590
53647556	53647579	X	-	27260157	MKN45	viability	CCAGTGCGAGTTATATCACTGGG	HUWE1	ENSG00000086758	-2.5750750296512	[]	[]	-9	hSpCas9	negative selection	2726024
53648247	53648270	X	-	27260157	MKN45	viability	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-1.24894155247162	[]	[]	-8	hSpCas9	negative selection	2726295
53645316	53645339	X	-	27260157	MKN45	viability	GCTAACTCGGCTACAACATTTGG	HUWE1	ENSG00000086758	-1.79006659460911	[]	[]	-9	hSpCas9	negative selection	2733960
53647529	53647552	X	+	27260157	MKN45	viability	TTCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-0.840490660437217	[]	[]	-7	hSpCas9	negative selection	2734059
53628796	53628819	X	-	27260157	MKN45	viability	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.13602515189888	[]	[]	-8	hSpCas9	negative selection	2738679
53629521	53629544	X	+	27260157	MKN45	viability	GCTGCTTATCCGTTATCTGAAGG	HUWE1	ENSG00000086758	-0.895694747281882	[]	[]	-7	hSpCas9	negative selection	2739201
53647513	53647536	X	-	27260157	MKN45	viability	GATGGAATACTGGCAGATGCTGG	HUWE1	ENSG00000086758	-1.77097969338528	[]	[]	-9	hSpCas9	negative selection	2758053
53647523	53647546	X	-	27260157	MKN45	viability	GGACCGCTTCGATGGAATACTGG	HUWE1	ENSG00000086758	-1.40927197498264	[]	[]	-8	hSpCas9	negative selection	2759241
53629596	53629619	X	-	27260157	MKN45	viability	AGTGTATTCCAATGCCTTGCAGG	HUWE1	ENSG00000086758	-1.36685026764832	[]	[]	-8	hSpCas9	negative selection	2759679
53647553	53647576	X	-	27260157	MKN45	viability	GTGCGAGTTATATCACTGGGTGG	HUWE1	ENSG00000086758	-3.18122165310195	[]	[]	-9	hSpCas9	negative selection	2760203
53648288	53648311	X	+	27260157	MKN45	viability	ATTAAGGCTCTGCAGTCTGCAGG	HUWE1	ENSG00000086758	-1.29664568402628	[]	[]	-8	hSpCas9	negative selection	2762665
53647520	53647543	X	+	27260157	MKN45	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.95302967256322	[]	[]	-7	hSpCas9	negative selection	2766451
53648272	53648295	X	+	27260157	MKN45	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.288539822544374	[]	[]	-1	hSpCas9	negative selection	2770381
53632536	53632559	X	+	27260157	MKN45	viability	TGTAGTGTAGTTGCACTGGGTGG	HUWE1	ENSG00000086758	-0.814689716736427	[]	[]	-7	hSpCas9	negative selection	2771245
53654093	53654116	X	-	27260157	MKN45	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.727245128217568	[]	[]	7	hSpCas9	negative selection	2772514
53628609	53628632	X	-	27260157	MKN45	viability	CTCCCTTGTAGATCCTTCCATGG	HUWE1	ENSG00000086758	-1.73476817055537	[]	[]	-9	hSpCas9	negative selection	2773276
53647470	53647493	X	-	27260157	MKN45	viability	CATGGATGCTCGTATGTGATAGG	HUWE1	ENSG00000086758	-1.99602480750352	[]	[]	-9	hSpCas9	negative selection	2776158
53647544	53647567	X	-	27260157	MKN45	viability	ATATCACTGGGTGGACCTGTTGG	HUWE1	ENSG00000086758	-1.13000335909205	[]	[]	-8	hSpCas9	negative selection	2776183
53630962	53630985	X	-	27260157	MKN45	viability	GAAGCGATTGCAGGCAGTTCAGG	HUWE1	ENSG00000086758	-0.37048553700824	[]	[]	-1	hSpCas9	negative selection	2794720
53647556	53647579	X	-	27260157	RKO	viability	CCAGTGCGAGTTATATCACTGGG	HUWE1	ENSG00000086758	-3.06126552698757	[]	[]	-9	hSpCas9	negative selection	2802154
53648247	53648270	X	-	27260157	RKO	viability	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-1.62053101521905	[]	[]	-9	hSpCas9	negative selection	2802425
53645316	53645339	X	-	27260157	RKO	viability	GCTAACTCGGCTACAACATTTGG	HUWE1	ENSG00000086758	-1.97155904655204	[]	[]	-9	hSpCas9	negative selection	2810090
53647529	53647552	X	+	27260157	RKO	viability	TTCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-1.54455400825516	[]	[]	-9	hSpCas9	negative selection	2810189
53628796	53628819	X	-	27260157	RKO	viability	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.43456453351539	[]	[]	-8	hSpCas9	negative selection	2814809
53629521	53629544	X	+	27260157	RKO	viability	GCTGCTTATCCGTTATCTGAAGG	HUWE1	ENSG00000086758	-2.01752804645538	[]	[]	-9	hSpCas9	negative selection	2815331
53647513	53647536	X	-	27260157	RKO	viability	GATGGAATACTGGCAGATGCTGG	HUWE1	ENSG00000086758	-1.83245696028901	[]	[]	-9	hSpCas9	negative selection	2834183
53647523	53647546	X	-	27260157	RKO	viability	GGACCGCTTCGATGGAATACTGG	HUWE1	ENSG00000086758	-1.44746069650059	[]	[]	-8	hSpCas9	negative selection	2835371
53629596	53629619	X	-	27260157	RKO	viability	AGTGTATTCCAATGCCTTGCAGG	HUWE1	ENSG00000086758	-1.91461373852425	[]	[]	-9	hSpCas9	negative selection	2835809
53647553	53647576	X	-	27260157	RKO	viability	GTGCGAGTTATATCACTGGGTGG	HUWE1	ENSG00000086758	-1.81623592240825	[]	[]	-9	hSpCas9	negative selection	2836333
53648288	53648311	X	+	27260157	RKO	viability	ATTAAGGCTCTGCAGTCTGCAGG	HUWE1	ENSG00000086758	-1.50681560609963	[]	[]	-8	hSpCas9	negative selection	2838795
53647520	53647543	X	+	27260157	RKO	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.25418911695125	[]	[]	-9	hSpCas9	negative selection	2842581
53648272	53648295	X	+	27260157	RKO	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	1.02787766557371	[]	[]	9	hSpCas9	negative selection	2846511
53632536	53632559	X	+	27260157	RKO	viability	TGTAGTGTAGTTGCACTGGGTGG	HUWE1	ENSG00000086758	-1.69222935470594	[]	[]	-9	hSpCas9	negative selection	2847375
53654093	53654116	X	-	27260157	RKO	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.202449019406708	[]	[]	1	hSpCas9	negative selection	2848644
53628609	53628632	X	-	27260157	RKO	viability	CTCCCTTGTAGATCCTTCCATGG	HUWE1	ENSG00000086758	-1.29674342000838	[]	[]	-8	hSpCas9	negative selection	2849406
53647470	53647493	X	-	27260157	RKO	viability	CATGGATGCTCGTATGTGATAGG	HUWE1	ENSG00000086758	-2.78015567985801	[]	[]	-9	hSpCas9	negative selection	2852288
53647544	53647567	X	-	27260157	RKO	viability	ATATCACTGGGTGGACCTGTTGG	HUWE1	ENSG00000086758	-1.07094098063975	[]	[]	-8	hSpCas9	negative selection	2852313
53630962	53630985	X	-	27260157	RKO	viability	GAAGCGATTGCAGGCAGTTCAGG	HUWE1	ENSG00000086758	-0.930988995778373	[]	[]	-8	hSpCas9	negative selection	2870850
53647556	53647579	X	-	27260157	SF268	viability	CCAGTGCGAGTTATATCACTGGG	HUWE1	ENSG00000086758	-3.49305797650392	[]	[]	-9	hSpCas9	negative selection	2878284
53648247	53648270	X	-	27260157	SF268	viability	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-2.39777233045539	[]	[]	-9	hSpCas9	negative selection	2878555
53645316	53645339	X	-	27260157	SF268	viability	GCTAACTCGGCTACAACATTTGG	HUWE1	ENSG00000086758	-2.96374221306547	[]	[]	-9	hSpCas9	negative selection	2886220
53647529	53647552	X	+	27260157	SF268	viability	TTCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-2.12635431706472	[]	[]	-9	hSpCas9	negative selection	2886319
53628796	53628819	X	-	27260157	SF268	viability	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.45901284677852	[]	[]	-8	hSpCas9	negative selection	2890939
53629521	53629544	X	+	27260157	SF268	viability	GCTGCTTATCCGTTATCTGAAGG	HUWE1	ENSG00000086758	-2.49403185667641	[]	[]	-9	hSpCas9	negative selection	2891461
53647513	53647536	X	-	27260157	SF268	viability	GATGGAATACTGGCAGATGCTGG	HUWE1	ENSG00000086758	-3.5332237641068	[]	[]	-9	hSpCas9	negative selection	2910313
53647523	53647546	X	-	27260157	SF268	viability	GGACCGCTTCGATGGAATACTGG	HUWE1	ENSG00000086758	-2.66408767114703	[]	[]	-9	hSpCas9	negative selection	2911501
53629596	53629619	X	-	27260157	SF268	viability	AGTGTATTCCAATGCCTTGCAGG	HUWE1	ENSG00000086758	-2.14830576266093	[]	[]	-9	hSpCas9	negative selection	2911939
53647553	53647576	X	-	27260157	SF268	viability	GTGCGAGTTATATCACTGGGTGG	HUWE1	ENSG00000086758	-2.80680604008555	[]	[]	-9	hSpCas9	negative selection	2912463
53648288	53648311	X	+	27260157	SF268	viability	ATTAAGGCTCTGCAGTCTGCAGG	HUWE1	ENSG00000086758	-2.91804299421635	[]	[]	-9	hSpCas9	negative selection	2914925
53647520	53647543	X	+	27260157	SF268	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.81124650710902	[]	[]	-9	hSpCas9	negative selection	2918711
53648272	53648295	X	+	27260157	SF268	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.406617439030791	[]	[]	-3	hSpCas9	negative selection	2922641
53632536	53632559	X	+	27260157	SF268	viability	TGTAGTGTAGTTGCACTGGGTGG	HUWE1	ENSG00000086758	-1.3556871903707	[]	[]	-7	hSpCas9	negative selection	2923505
53654093	53654116	X	-	27260157	SF268	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.228653055597114	[]	[]	-2	hSpCas9	negative selection	2924774
53628609	53628632	X	-	27260157	SF268	viability	CTCCCTTGTAGATCCTTCCATGG	HUWE1	ENSG00000086758	-3.68889410539921	[]	[]	-9	hSpCas9	negative selection	2925536
53647470	53647493	X	-	27260157	SF268	viability	CATGGATGCTCGTATGTGATAGG	HUWE1	ENSG00000086758	-2.92692624894121	[]	[]	-9	hSpCas9	negative selection	2928418
53647544	53647567	X	-	27260157	SF268	viability	ATATCACTGGGTGGACCTGTTGG	HUWE1	ENSG00000086758	-2.21513804636776	[]	[]	-9	hSpCas9	negative selection	2928443
53630962	53630985	X	-	27260157	SF268	viability	GAAGCGATTGCAGGCAGTTCAGG	HUWE1	ENSG00000086758	-1.7595169144182	[]	[]	-8	hSpCas9	negative selection	2946980
53617077	53617100	X	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCACTCTGTTTGAATGCCCGAGG	HUWE1	ENSG00000086758	-0.4336263703329226	[2,2]	[2,0]	-6	hSpCas9	positive selection	2994563
53614634	53614657	X	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCATGATTAGACCTTGGGGGAGG	HUWE1	ENSG00000086758	-0.019797632165497236	[4,4]	[2,4]	0	hSpCas9	positive selection	2994564
53624633	53624656	X	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCTGATGATGTGTTTCAGGGAGG	HUWE1	ENSG00000086758	-0.40561256704373966	[2,2]	[1,1]	-5	hSpCas9	positive selection	2994565
53592416	53592439	X	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GGTTGACTTGAGGTTCCCGGCGG	HUWE1	ENSG00000086758	0.19498768243493825	[2,2]	[2,1]	4	hSpCas9	positive selection	2994566
53617077	53617100	X	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCACTCTGTTTGAATGCCCGAGG	HUWE1	ENSG00000086758	-1.1175845715767592	[2,2]	[0,0]	-9	hSpCas9	positive selection	3062421
53614634	53614657	X	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCATGATTAGACCTTGGGGGAGG	HUWE1	ENSG00000086758	-0.7412347038160039	[4,4]	[2,0]	-8	hSpCas9	positive selection	3062422
53624633	53624656	X	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCTGATGATGTGTTTCAGGGAGG	HUWE1	ENSG00000086758	0.5157640244171726	[2,2]	[1,2]	8	hSpCas9	positive selection	3062423
53592416	53592439	X	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GGTTGACTTGAGGTTCCCGGCGG	HUWE1	ENSG00000086758	0.25303528386538715	[2,2]	[1,1]	4	hSpCas9	positive selection	3062424
53648272	53648295		+	24336571	A375	resistance to PLX after 7 days	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.23418089548810228	[7,9]	[7,6]	-7	hSpCas9	positive selection	3120146
53654065	53654088		-	24336571	A375	resistance to PLX after 7 days	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	0.36818516207751806	[3,5]	[5,6]	8	hSpCas9	positive selection	3120147
53648247	53648270		-	24336571	A375	resistance to PLX after 7 days	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	0.26491845798710606	[7,11]	[11,11]	7	hSpCas9	positive selection	3120148
53654093	53654116		-	24336571	A375	resistance to PLX after 7 days	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.1120808933227212	[7,6]	[6,5]	-3	hSpCas9	positive selection	3120149
53648272	53648295		+	24336571	A375	resistance to PLX after 14 days	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.9646275769578401	[8,9]	[1,1]	-9	hSpCas9	positive selection	3177939
53654065	53654088		-	24336571	A375	resistance to PLX after 14 days	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	0.5251065195749565	[2,3]	[2,1]	5	hSpCas9	positive selection	3177940
53648247	53648270		-	24336571	A375	resistance to PLX after 14 days	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	0.6544932135789725	[9,8]	[7,4]	6	hSpCas9	positive selection	3177941
53654093	53654116		-	24336571	A375	resistance to PLX after 14 days	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.709201048483111	[5,5]	[3,4]	6	hSpCas9	positive selection	3177942
53648272	53648295		+	24336571	A375	viability after 7 days	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.05336606390332305	[9]	[7,9]	0	hSpCas9	negative selection	3235732
53654065	53654088		-	24336571	A375	viability after 7 days	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.2203939624876277	[12]	[3,5]	-9	hSpCas9	negative selection	3235733
53648247	53648270		-	24336571	A375	viability after 7 days	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-0.13803676839615883	[11]	[7,11]	-2	hSpCas9	negative selection	3235734
53654093	53654116		-	24336571	A375	viability after 7 days	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.391873570026439	[9]	[7,6]	-5	hSpCas9	negative selection	3235735
53648272	53648295		+	24336571	A375	viability after 14 days	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.06546228972433721	[9]	[8,9]	0	hSpCas9	negative selection	3293525
53654065	53654088		-	24336571	A375	viability after 14 days	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.5442046305896648	[12]	[2,3]	-9	hSpCas9	negative selection	3293526
53648247	53648270		-	24336571	A375	viability after 14 days	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-0.18428231930938413	[11]	[9,8]	-2	hSpCas9	negative selection	3293527
53654093	53654116		-	24336571	A375	viability after 14 days	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.6540651122322223	[9]	[5,5]	-7	hSpCas9	negative selection	3293528
53648272	53648295	X	+	27383988	293T	resistance to West Nile virus (flavivirus)	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.08740121000352279	[143,143]	[0,0]	0	hSpCas9	positive selection	3373573
53648224	53648247	X	-	27383988	293T	resistance to West Nile virus (flavivirus)	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	0.46291034509572904	[110,110]	[0,0]	2	hSpCas9	positive selection	3373574
53654093	53654116	X	-	27383988	293T	resistance to West Nile virus (flavivirus)	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.07741712143090065	[144,144]	[0,0]	0	hSpCas9	positive selection	3373575
53617077	53617100	X	-	26780180	HT29	viability (Avana library 4 designs)	GCACTCTGTTTGAATGCCCGAGG	HUWE1	ENSG00000086758	3.876082791825	[]	[]	9	hSpCas9	negative selection	3431897
53614633	53614656	X	+	26780180	HT29	viability (Avana library 4 designs)	GCATGATTAGACCTTGGGGGAGG	HUWE1	ENSG00000086758	1.43582578962735	[]	[]	5	hSpCas9	negative selection	3431898
53624632	53624655	X	+	26780180	HT29	viability (Avana library 4 designs)	GCTGATGATGTGTTTCAGGGAGG	HUWE1	ENSG00000086758	3.7895337033172	[]	[]	9	hSpCas9	negative selection	3431899
53592415	53592438	X	+	26780180	HT29	viability (Avana library 4 designs)	GGTTGACTTGAGGTTCCCGGCGG	HUWE1	ENSG00000086758	3.37251113225708	[]	[]	8	hSpCas9	negative selection	3431900
53592415	53592438	X	+	26780180	A375	viability (Avana lentiCRISPRv2)	GGTTGACTTGAGGTTCCCGGCGG	HUWE1	ENSG00000086758	-12.0013472419209	[]	[]	-8	hSpCas9	negative selection	3517786
53624632	53624655	X	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCTGATGATGTGTTTCAGGGAGG	HUWE1	ENSG00000086758	-14.4978241938999	[]	[]	-9	hSpCas9	negative selection	3517787
53617077	53617100	X	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCACTCTGTTTGAATGCCCGAGG	HUWE1	ENSG00000086758	-6.58282747809181	[]	[]	-6	hSpCas9	negative selection	3517788
53614633	53614656	X	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCATGATTAGACCTTGGGGGAGG	HUWE1	ENSG00000086758	2.22702885792518	[]	[]	3	hSpCas9	negative selection	3517789
53647367	53647390	X	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCATCTGTACAGTTCCATAGAGG	HUWE1	ENSG00000086758	-9.85214433268662	[]	[]	-7	hSpCas9	negative selection	3517790
53580942	53580965	X	+	26780180	A375	viability (Avana lentiCRISPRv2)	AGTTGATCTCCCGAGAGCCGAGG	HUWE1	ENSG00000086758	-5.44716708376077	[]	[]	-5	hSpCas9	negative selection	3517791
53592415	53592438	X	+	26780180	A375	viability (Avana lentiGuide)	GGTTGACTTGAGGTTCCCGGCGG	HUWE1	ENSG00000086758	16.7197478939478	[]	[]	8	hSpCas9	negative selection	3626445
53624632	53624655	X	+	26780180	A375	viability (Avana lentiGuide)	GCTGATGATGTGTTTCAGGGAGG	HUWE1	ENSG00000086758	24.0771448732991	[]	[]	9	hSpCas9	negative selection	3626446
53617077	53617100	X	-	26780180	A375	viability (Avana lentiGuide)	GCACTCTGTTTGAATGCCCGAGG	HUWE1	ENSG00000086758	22.5506146968924	[]	[]	9	hSpCas9	negative selection	3626447
53614633	53614656	X	+	26780180	A375	viability (Avana lentiGuide)	GCATGATTAGACCTTGGGGGAGG	HUWE1	ENSG00000086758	5.63021696681841	[]	[]	5	hSpCas9	negative selection	3626448
53647367	53647390	X	-	26780180	A375	viability (Avana lentiGuide)	GCATCTGTACAGTTCCATAGAGG	HUWE1	ENSG00000086758	27.8195236512015	[]	[]	9	hSpCas9	negative selection	3626449
53580942	53580965	X	+	26780180	A375	viability (Avana lentiGuide)	AGTTGATCTCCCGAGAGCCGAGG	HUWE1	ENSG00000086758	8.61400727021143	[]	[]	6	hSpCas9	negative selection	3626450
53592415	53592438	X	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GGTTGACTTGAGGTTCCCGGCGG	HUWE1	ENSG00000086758	-1.0619857678301794	[3]	[0,1]	-9	hSpCas9	positive selection	3735104
53624632	53624655	X	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCTGATGATGTGTTTCAGGGAGG	HUWE1	ENSG00000086758	-0.6968881064538219	[4]	[2,1]	-7	hSpCas9	positive selection	3735105
53617077	53617100	X	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCACTCTGTTTGAATGCCCGAGG	HUWE1	ENSG00000086758	-0.2941673669336742	[4]	[3,2]	-4	hSpCas9	positive selection	3735106
53614633	53614656	X	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCATGATTAGACCTTGGGGGAGG	HUWE1	ENSG00000086758	0.2714584610126195	[5]	[6,6]	7	hSpCas9	positive selection	3735107
53647367	53647390	X	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCATCTGTACAGTTCCATAGAGG	HUWE1	ENSG00000086758	-0.22482367430614852	[5]	[4,3]	-4	hSpCas9	positive selection	3735108
53580942	53580965	X	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AGTTGATCTCCCGAGAGCCGAGG	HUWE1	ENSG00000086758	-0.07916967837548262	[4]	[4,4]	-1	hSpCas9	positive selection	3735109
53592415	53592438	X	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GGTTGACTTGAGGTTCCCGGCGG	HUWE1	ENSG00000086758	-0.7620945436156022	[5]	[2,1]	-7	hSpCas9	positive selection	3843711
53624632	53624655	X	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCTGATGATGTGTTTCAGGGAGG	HUWE1	ENSG00000086758	-1.0587509295538091	[4]	[0,1]	-8	hSpCas9	positive selection	3843712
53617077	53617100	X	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCACTCTGTTTGAATGCCCGAGG	HUWE1	ENSG00000086758	-0.864347052668496	[4]	[3,0]	-8	hSpCas9	positive selection	3843713
53614633	53614656	X	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCATGATTAGACCTTGGGGGAGG	HUWE1	ENSG00000086758	0.10372035099560137	[6]	[5,5]	2	hSpCas9	positive selection	3843714
53647367	53647390	X	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCATCTGTACAGTTCCATAGAGG	HUWE1	ENSG00000086758	-0.3475309466205275	[5]	[4,2]	-4	hSpCas9	positive selection	3843715
53580942	53580965	X	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AGTTGATCTCCCGAGAGCCGAGG	HUWE1	ENSG00000086758	-0.4299904670921701	[4]	[3,1]	-5	hSpCas9	positive selection	3843716
53648224	53648247	X	-	26780180	A375	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	2.17798046660761	[]	[]	9	hSpCas9	negative selection	3949684
53648272	53648295	X	+	26780180	A375	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	1.24275000498417	[]	[]	6	hSpCas9	negative selection	3949685
53654065	53654088	X	-	26780180	A375	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	2.04178445550931	[]	[]	9	hSpCas9	negative selection	3949686
53647520	53647543	X	+	26780180	A375	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	1.16458406402262	[]	[]	6	hSpCas9	negative selection	3949687
53647531	53647554	X	-	26780180	A375	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	1.61972657729093	[]	[]	8	hSpCas9	negative selection	3949688
53654093	53654116	X	-	26780180	A375	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	1.44697619722099	[]	[]	8	hSpCas9	negative selection	3949689
53648224	53648247	X	-	26780180	HT29	viability (GeCKOv2 library)	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	1.97467827633769	[]	[]	9	hSpCas9	negative selection	4057650
53648272	53648295	X	+	26780180	HT29	viability (GeCKOv2 library)	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.445219653268706	[]	[]	1	hSpCas9	negative selection	4057651
53654065	53654088	X	-	26780180	HT29	viability (GeCKOv2 library)	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	1.1572944907242	[]	[]	5	hSpCas9	negative selection	4057652
53647520	53647543	X	+	26780180	HT29	viability (GeCKOv2 library)	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	1.35408124281713	[]	[]	7	hSpCas9	negative selection	4057653
53647531	53647554	X	-	26780180	HT29	viability (GeCKOv2 library)	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	0.902040266974929	[]	[]	4	hSpCas9	negative selection	4057654
53654093	53654116	X	-	26780180	HT29	viability (GeCKOv2 library)	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	1.83836232535457	[]	[]	8	hSpCas9	negative selection	4057655
53648272	53648295	X	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.4584200382501673	[2]	[1,0]	-8	hSpCas9	positive selection	4143731
53654065	53654088	X	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.5163445945622889	[2]	[0,0]	-8	hSpCas9	positive selection	4143732
53648247	53648270	X	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	0.04327211004736336	[3]	[1,2]	1	hSpCas9	positive selection	4143733
53654093	53654116	X	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.2611267340235187	[2]	[1,1]	6	hSpCas9	positive selection	4143734
53648272	53648295	X	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.2832211076503633	[3]	[0,0,0,2]	2	hSpCas9	positive selection	4207753
53654065	53654088	X	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	0.3975412359304325	[3]	[0,0,0,2]	3	hSpCas9	positive selection	4207754
53648247	53648270	X	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TAATGATGAGCAACTTCTCTTGG	HUWE1	ENSG00000086758	-0.4451139260120137	[4]	[1,2,0,0]	-6	hSpCas9	positive selection	4207755
53654093	53654116	X	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.7266765681373252	[3]	[0,0,0,0]	-8	hSpCas9	positive selection	4207756
53647531	53647554	X	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.0984644493899324	[3]	[2,1]	-2	hSpCas9	positive selection	4276110
53647520	53647543	X	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.4230237517704284	[1]	[0,0]	-7	hSpCas9	positive selection	4276111
53654065	53654088	X	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.23929204744176827	[3]	[2,1]	-5	hSpCas9	positive selection	4276112
53648272	53648295	X	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.27947152626282185	[2]	[3,1]	5	hSpCas9	positive selection	4340368
53648224	53648247	X	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.519723634065279	[3]	[2,0]	-8	hSpCas9	positive selection	4340369
53654093	53654116	X	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.5089030415669014	[2]	[1,0]	-8	hSpCas9	positive selection	4340370
53575679	53575701	X	+	27760321	OCIAML3	viability	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.8637044629875317	[456,428]	[96,92]	-9	hSpCas9	negative selection	4408487
53583833	53583855	X	-	27760321	OCIAML3	viability	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-0.5777166643537253	[499,363]	[208,243]	-5	hSpCas9	negative selection	4408488
53585052	53585074	X	-	27760321	OCIAML3	viability	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-3.717771239846713	[554,438]	[34,21]	-9	hSpCas9	negative selection	4408489
53645324	53645346	X	+	27760321	OCIAML3	viability	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-2.563739471915089	[115,119]	[6,26]	-9	hSpCas9	negative selection	4408490
53647530	53647552	X	+	27760321	OCIAML3	viability	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-2.2394600470943886	[458,430]	[81,62]	-9	hSpCas9	negative selection	4408491
53575679	53575701	X	+	27760321	OCIAML2	viability	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.499080421612504	[456,428]	[103,156]	-8	hSpCas9	negative selection	4491950
53583833	53583855	X	-	27760321	OCIAML2	viability	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-0.014167130437623542	[499,363]	[299,398]	0	hSpCas9	negative selection	4491951
53585052	53585074	X	-	27760321	OCIAML2	viability	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-2.1832488426887147	[554,438]	[71,108]	-9	hSpCas9	negative selection	4491952
53645324	53645346	X	+	27760321	OCIAML2	viability	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.1959115241601974	[115,119]	[40,42]	-8	hSpCas9	negative selection	4491953
53647530	53647552	X	+	27760321	OCIAML2	viability	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-0.8811662158005782	[458,430]	[151,252]	-7	hSpCas9	negative selection	4491954
53575679	53575701	X	+	27760321	MV411	viability	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.2341020417135524	[456,428]	[75,196]	-7	hSpCas9	negative selection	4575413
53583833	53583855	X	-	27760321	MV411	viability	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-0.6923804763415227	[499,363]	[71,328]	-5	hSpCas9	negative selection	4575414
53585052	53585074	X	-	27760321	MV411	viability	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-4.998894356924068	[554,438]	[0,23]	-9	hSpCas9	negative selection	4575415
53645324	53645346	X	+	27760321	MV411	viability	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.9396305752876384	[115,119]	[25,62]	-6	hSpCas9	negative selection	4575416
53647530	53647552	X	+	27760321	MV411	viability	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-1.800793377033741	[458,430]	[15,186]	-8	hSpCas9	negative selection	4575417
53575679	53575701	X	+	27760321	HL60	viability	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-0.9272885178317305	[456,428]	[220,157]	-7	hSpCas9	negative selection	4658876
53583833	53583855	X	-	27760321	HL60	viability	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-0.48274465627940477	[499,363]	[269,228]	-5	hSpCas9	negative selection	4658877
53585052	53585074	X	-	27760321	HL60	viability	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-1.757413411327558	[554,438]	[96,142]	-9	hSpCas9	negative selection	4658878
53645324	53645346	X	+	27760321	HL60	viability	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-6.597563979792381	[115,119]	[0,0]	-9	hSpCas9	negative selection	4658879
53647530	53647552	X	+	27760321	HL60	viability	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-1.854942473182358	[458,430]	[85,115]	-9	hSpCas9	negative selection	4658880
53575679	53575701	X	+	27760321	HT1080	viability	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.0389844861980795	[428,334]	[106,37]	-4	hSpCas9	negative selection	4742339
53583833	53583855	X	-	27760321	HT1080	viability	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-3.4354449955146005	[363,283]	[20,1]	-7	hSpCas9	negative selection	4742340
53585052	53585074	X	-	27760321	HT1080	viability	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-7.2833058192363325	[438,342]	[0,0]	-9	hSpCas9	negative selection	4742341
53645324	53645346	X	+	27760321	HT1080	viability	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.47346714816966307	[119,93]	[13,48]	-2	hSpCas9	negative selection	4742342
53647530	53647552	X	+	27760321	HT1080	viability	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-4.852361265832913	[430,336]	[0,9]	-8	hSpCas9	negative selection	4742343
53575679	53575701	X	+	27760321	HT29	viability after 7 days	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-0.6711724973348495	[428,334]	[342,315,271]	-7	hSpCas9	negative selection	4825802
53583833	53583855	X	-	27760321	HT29	viability after 7 days	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	0.3311392398870029	[363,283]	[317,795,430]	5	hSpCas9	negative selection	4825803
53585052	53585074	X	-	27760321	HT29	viability after 7 days	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-0.5998052829503272	[438,342]	[466,214,331]	-7	hSpCas9	negative selection	4825804
53645324	53645346	X	+	27760321	HT29	viability after 7 days	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	0.06527805350495597	[119,93]	[177,223,37]	1	hSpCas9	negative selection	4825805
53647530	53647552	X	+	27760321	HT29	viability after 7 days	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-0.5855710789114809	[430,336]	[462,189,351]	-7	hSpCas9	negative selection	4825806
53575679	53575701	X	+	27760321	HT29	viability after 25 days	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.6269038612141633	[428,334]	[112,193,115]	-7	hSpCas9	negative selection	4909265
53583833	53583855	X	-	27760321	HT29	viability after 25 days	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	0.12378481104302345	[363,283]	[367,439,403]	1	hSpCas9	negative selection	4909266
53585052	53585074	X	-	27760321	HT29	viability after 25 days	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-1.6572408952135214	[438,342]	[135,217,62]	-7	hSpCas9	negative selection	4909267
53645324	53645346	X	+	27760321	HT29	viability after 25 days	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.2944581572904852	[119,93]	[146,135,2]	-3	hSpCas9	negative selection	4909268
53647530	53647552	X	+	27760321	HT29	viability after 25 days	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-1.7973947554969967	[430,336]	[55,157,173]	-8	hSpCas9	negative selection	4909269
53575679	53575701	X	+	27760321	HT29	viability after 22 days	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.9952141640116974	[428,334]	[74,176,80]	-8	hSpCas9	negative selection	4992728
53583833	53583855	X	-	27760321	HT29	viability after 22 days	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-0.10765099837528061	[363,283]	[222,393,429]	-1	hSpCas9	negative selection	4992729
53585052	53585074	X	-	27760321	HT29	viability after 22 days	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-1.5843089767087501	[438,342]	[124,170,156]	-7	hSpCas9	negative selection	4992730
53645324	53645346	X	+	27760321	HT29	viability after 22 days	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.31004469295044945	[119,93]	[48,231,18]	-3	hSpCas9	negative selection	4992731
53647530	53647552	X	+	27760321	HT29	viability after 22 days	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-2.251280009035021	[430,336]	[31,93,155]	-8	hSpCas9	negative selection	4992732
53575679	53575701	X	+	27760321	HT29	viability after 19 days	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.1353772880325215	[428,334]	[88,135,353]	-7	hSpCas9	negative selection	5076191
53583833	53583855	X	-	27760321	HT29	viability after 19 days	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-0.4479997438470632	[363,283]	[499,130,209]	-4	hSpCas9	negative selection	5076192
53585052	53585074	X	-	27760321	HT29	viability after 19 days	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-2.2371798456686034	[438,342]	[84,117,80]	-8	hSpCas9	negative selection	5076193
53645324	53645346	X	+	27760321	HT29	viability after 19 days	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.6335686235659805	[119,93]	[14,190,25]	-5	hSpCas9	negative selection	5076194
53647530	53647552	X	+	27760321	HT29	viability after 19 days	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-2.0960651391784877	[430,336]	[101,101,103]	-8	hSpCas9	negative selection	5076195
53575679	53575701	X	+	27760321	HT29	viability after 16 days	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-0.7696304175657583	[428,334]	[271,99,385]	-6	hSpCas9	negative selection	5159654
53583833	53583855	X	-	27760321	HT29	viability after 16 days	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	0.2667276879613836	[363,283]	[495,354,464]	3	hSpCas9	negative selection	5159655
53585052	53585074	X	-	27760321	HT29	viability after 16 days	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-1.9992005739670418	[438,342]	[89,87,148]	-8	hSpCas9	negative selection	5159656
53645324	53645346	X	+	27760321	HT29	viability after 16 days	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.9207893013357422	[119,93]	[126,53,14]	-7	hSpCas9	negative selection	5159657
53647530	53647552	X	+	27760321	HT29	viability after 16 days	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-1.2927595076610212	[430,336]	[235,168,126]	-7	hSpCas9	negative selection	5159658
53575679	53575701	X	+	27760321	HT29	viability after 13 days	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-1.2569628524100687	[428,334]	[227,207,123]	-8	hSpCas9	negative selection	5243117
53583833	53583855	X	-	27760321	HT29	viability after 13 days	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	0.3591530819577182	[363,283]	[362,438,634]	4	hSpCas9	negative selection	5243118
53585052	53585074	X	-	27760321	HT29	viability after 13 days	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-1.2901785675772852	[438,342]	[299,135,123]	-8	hSpCas9	negative selection	5243119
53645324	53645346	X	+	27760321	HT29	viability after 13 days	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.6086247567740228	[119,93]	[46,92,102]	-6	hSpCas9	negative selection	5243120
53647530	53647552	X	+	27760321	HT29	viability after 13 days	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-0.844338459974176	[430,336]	[258,230,253]	-7	hSpCas9	negative selection	5243121
53575679	53575701	X	+	27760321	HT29	viability after 10 days	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-0.6534344702001482	[428,334]	[182,443,286]	-6	hSpCas9	negative selection	5326580
53583833	53583855	X	-	27760321	HT29	viability after 10 days	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	0.3793699128442404	[363,283]	[587,668,329]	5	hSpCas9	negative selection	5326581
53585052	53585074	X	-	27760321	HT29	viability after 10 days	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-1.5303796084029964	[438,342]	[221,144,139]	-8	hSpCas9	negative selection	5326582
53645324	53645346	X	+	27760321	HT29	viability after 10 days	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.3368266963017521	[119,93]	[170,148,0]	-4	hSpCas9	negative selection	5326583
53647530	53647552	X	+	27760321	HT29	viability after 10 days	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-0.9851992394941599	[430,336]	[174,379,176]	-8	hSpCas9	negative selection	5326584
53575679	53575701	X	+	27760321	MOLM13	viability	GCTCTGACGCGTAAGTGACCGG	HUWE1	ENSG00000086758	-2.2080520035661637	[456,428]	[85,26]	-8	hSpCas9	negative selection	5410043
53583833	53583855	X	-	27760321	MOLM13	viability	ATCGGGGAGATCCTGATCCAGG	HUWE1	ENSG00000086758	-0.12380920663770942	[499,363]	[247,198]	-1	hSpCas9	negative selection	5410044
53585052	53585074	X	-	27760321	MOLM13	viability	CAAGCGTGCGATTCACTGCAGG	HUWE1	ENSG00000086758	-2.884579889550014	[554,438]	[0,68]	-8	hSpCas9	negative selection	5410045
53645324	53645346	X	+	27760321	MOLM13	viability	TGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	0.9885272671541394	[115,119]	[223,54]	7	hSpCas9	negative selection	5410046
53647530	53647552	X	+	27760321	MOLM13	viability	TCCATCGAAGCGGTCCAACAGG	HUWE1	ENSG00000086758	-2.770076442059094	[458,430]	[65,11]	-8	hSpCas9	negative selection	5410047
53683552	53683575	X	+	27661255	K562	viability	GCCCACTGCGGCGGCGACAACGG	HUWE1	ENSG00000086758	-0.6670924228719808	[285,369]	[187,143]	-8	dCas9-KRAB	negative selection	5485904
53683574	53683597	X	+	27661255	K562	viability	GAGGCGTTGACCGATCGGTGGGG	HUWE1	ENSG00000086758	-0.39631901889651633	[1031,944]	[696,518]	-7	dCas9-KRAB	negative selection	5485905
53683595	53683618	X	+	27661255	K562	viability	GGCAAAGGCGGGCCCACACGCGG	HUWE1	ENSG00000086758	-0.5775604072130314	[328,358]	[212,158]	-8	dCas9-KRAB	negative selection	5485906
53683921	53683944	X	+	27661255	K562	viability	GAGAATTCTCCGGCTTAGAACGG	HUWE1	ENSG00000086758	-0.3479434304132602	[812,970]	[597,523]	-7	dCas9-KRAB	negative selection	5485907
53683882	53683905	X	-	27661255	K562	viability	GTCCTCGTGGGATTCGTTGGCGG	HUWE1	ENSG00000086758	-0.1283239998277702	[441,453]	[377,283]	-4	dCas9-KRAB	negative selection	5485908
53647531	53647554	X	-	27260156	BXPC3	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.638888610920701	[595]	[381,454,398,354]	-8	hSpCas9	negative selection	5548570
53647520	53647543	X	+	27260156	BXPC3	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.9532525103009262	[131]	[59,64,61,102]	-9	hSpCas9	negative selection	5548571
53654065	53654088	X	-	27260156	BXPC3	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.8143680400593544	[737]	[445,447,353,509]	-9	hSpCas9	negative selection	5548572
53648272	53648295	X	+	27260156	BXPC3	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.1675397644327341	[346]	[349,356,527,402]	3	hSpCas9	negative selection	5612828
53648224	53648247	X	-	27260156	BXPC3	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.7526390727973054	[614]	[397,314,365,455]	-9	hSpCas9	negative selection	5612829
53654093	53654116	X	-	27260156	BXPC3	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.2028921155031394	[420]	[381,447,249,448]	-5	hSpCas9	negative selection	5612830
53647531	53647554	X	-	27260156	A673	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.6728750146974336	[595]	[493,446,413,444]	-9	hSpCas9	negative selection	5669821
53647520	53647543	X	+	27260156	A673	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.5200872749625136	[131]	[110,97,80,154]	-8	hSpCas9	negative selection	5669822
53654065	53654088	X	-	27260156	A673	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.5860451765708158	[737]	[595,653,485,625]	-8	hSpCas9	negative selection	5669823
53648272	53648295	X	+	27260156	A673	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.3274892209616648	[346]	[633,440,585,438]	7	hSpCas9	negative selection	5734079
53648224	53648247	X	-	27260156	A673	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.9229030398186752	[614]	[428,322,336,479]	-9	hSpCas9	negative selection	5734080
53654093	53654116	X	-	27260156	A673	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.5208323183320571	[420]	[313,352,323,416]	-8	hSpCas9	negative selection	5734081
53647531	53647554	X	-	27260156	A375	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.57159318410111	[595]	[348,318,96,188]	-9	hSpCas9	negative selection	5791072
53647520	53647543	X	+	27260156	A375	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.0856503906962622	[131]	[39,17,60,24]	-9	hSpCas9	negative selection	5791073
53654065	53654088	X	-	27260156	A375	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.5124978492426147	[737]	[375,120,210,455]	-9	hSpCas9	negative selection	5791074
53648272	53648295	X	+	27260156	A375	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.5215957301330959	[346]	[505,838,560,467]	7	hSpCas9	negative selection	5855330
53648224	53648247	X	-	27260156	A375	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-2.176574939631466	[614]	[174,239,133,103]	-9	hSpCas9	negative selection	5855331
53654093	53654116	X	-	27260156	A375	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.1945351705667197	[420]	[464,515,416,341]	-3	hSpCas9	negative selection	5855332
53647531	53647554	X	-	27260156	COLO741	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.3860882439231695	[595]	[534,598,606]	-7	hSpCas9	negative selection	5912323
53647520	53647543	X	+	27260156	COLO741	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.6832451411021923	[131]	[83,191,41]	-8	hSpCas9	negative selection	5912324
53654065	53654088	X	-	27260156	COLO741	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.9255699514985887	[737]	[478,472,531]	-9	hSpCas9	negative selection	5912325
53648272	53648295	X	+	27260156	COLO741	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.21037814273344407	[346]	[411,367,371]	-4	hSpCas9	negative selection	5976581
53648224	53648247	X	-	27260156	COLO741	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.8187062985611755	[614]	[527,405,410]	-9	hSpCas9	negative selection	5976582
53654093	53654116	X	-	27260156	COLO741	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.1233209286015714	[420]	[476,660,351]	-3	hSpCas9	negative selection	5976583
53647531	53647554	X	-	27260156	CAL120	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.5820619269889922	[595]	[545,580,368]	-7	hSpCas9	negative selection	6033574
53647520	53647543	X	+	27260156	CAL120	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.8316747887588836	[131]	[40,12,17]	-9	hSpCas9	negative selection	6033575
53654065	53654088	X	-	27260156	CAL120	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.9041865347305329	[737]	[187,390,840]	-8	hSpCas9	negative selection	6033576
53648272	53648295	X	+	27260156	CAL120	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.6612120863221713	[346]	[849,683,541]	8	hSpCas9	negative selection	6097832
53648224	53648247	X	-	27260156	CAL120	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.4354361424653712	[614]	[301,231,316]	-9	hSpCas9	negative selection	6097833
53654093	53654116	X	-	27260156	CAL120	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.06565942999077369	[420]	[497,587,550]	1	hSpCas9	negative selection	6097834
53647531	53647554	X	-	27260156	CADOES1	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.5750198764393879	[595]	[310,442,303,432]	-7	hSpCas9	negative selection	6154825
53647520	53647543	X	+	27260156	CADOES1	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.491128001128503	[131]	[34,23,37,78]	-9	hSpCas9	negative selection	6154826
53654065	53654088	X	-	27260156	CADOES1	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.1615833962685238	[737]	[328,215,401,278]	-9	hSpCas9	negative selection	6154827
53648272	53648295	X	+	27260156	CADOES1	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.5133932830403759	[346]	[441,582,407,407]	8	hSpCas9	negative selection	6219083
53648224	53648247	X	-	27260156	CADOES1	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.1959278061544651	[614]	[263,225,299,206]	-9	hSpCas9	negative selection	6219084
53654093	53654116	X	-	27260156	CADOES1	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.23834736997244538	[420]	[468,521,437,416]	4	hSpCas9	negative selection	6219085
53647531	53647554	X	-	27260156	EWS502	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.7345643861728713	[595]	[373,278,463,406]	-8	hSpCas9	negative selection	6276076
53647520	53647543	X	+	27260156	EWS502	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.4607419709783038	[131]	[17,65,81,39]	-9	hSpCas9	negative selection	6276077
53654065	53654088	X	-	27260156	EWS502	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.5670101132137044	[737]	[263,221,234,339]	-9	hSpCas9	negative selection	6276078
53648272	53648295	X	+	27260156	EWS502	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.38092702039641224	[346]	[617,468,374,428]	6	hSpCas9	negative selection	6340334
53648224	53648247	X	-	27260156	EWS502	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.283809688989125	[614]	[283,318,208,250]	-9	hSpCas9	negative selection	6340335
53654093	53654116	X	-	27260156	EWS502	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.166671209849247	[420]	[279,333,580,412]	-3	hSpCas9	negative selection	6340336
53647531	53647554	X	-	27260156	EW8	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.5262263090454851	[595]	[325,326,307,382]	-7	hSpCas9	negative selection	6397327
53647520	53647543	X	+	27260156	EW8	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.7512137036146	[131]	[128,61,53,17]	-8	hSpCas9	negative selection	6397328
53654065	53654088	X	-	27260156	EW8	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.088001967671035	[737]	[273,350,258,243]	-9	hSpCas9	negative selection	6397329
53648272	53648295	X	+	27260156	EW8	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.4895412746213519	[346]	[559,360,325,360]	8	hSpCas9	negative selection	6461585
53648224	53648247	X	-	27260156	EW8	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.980069501601077	[614]	[206,287,156,368]	-9	hSpCas9	negative selection	6461586
53654093	53654116	X	-	27260156	EW8	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.041560038851918446	[420]	[221,343,407,325]	-1	hSpCas9	negative selection	6461587
53647531	53647554	X	-	27260156	CORL105	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.5742186967628773	[595]	[592,552,400,319]	-8	hSpCas9	negative selection	6518578
53647520	53647543	X	+	27260156	CORL105	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.7585210692160025	[131]	[41,15,26,94]	-9	hSpCas9	negative selection	6518579
53654065	53654088	X	-	27260156	CORL105	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.2196832366024069	[737]	[438,310,337,393]	-9	hSpCas9	negative selection	6518580
53648272	53648295	X	+	27260156	CORL105	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.3896673201007344	[346]	[394,487,750,524]	7	hSpCas9	negative selection	6582836
53648224	53648247	X	-	27260156	CORL105	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.8903170366228835	[614]	[486,433,321,302]	-9	hSpCas9	negative selection	6582837
53654093	53654116	X	-	27260156	CORL105	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.1993378690038083	[420]	[394,316,539,482]	-4	hSpCas9	negative selection	6582838
53647531	53647554	X	-	27260156	HS294T	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.709693121264136	[595]	[103,75,222,170]	-7	hSpCas9	negative selection	6639829
53647520	53647543	X	+	27260156	HS294T	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.090260009940147	[131]	[16,13,20,48]	-9	hSpCas9	negative selection	6639830
53654065	53654088	X	-	27260156	HS294T	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.1469041947799494	[737]	[92,95,159,178]	-9	hSpCas9	negative selection	6639831
53648272	53648295	X	+	27260156	HS294T	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.5068478006147419	[346]	[140,156,153,340]	6	hSpCas9	negative selection	6704087
53648224	53648247	X	-	27260156	HS294T	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.15369143600393	[614]	[68,54,129,192]	-9	hSpCas9	negative selection	6704088
53654093	53654116	X	-	27260156	HS294T	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.47393978502275164	[420]	[76,178,72,145]	-6	hSpCas9	negative selection	6704089
53647531	53647554	X	-	27260156	HCC44	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.7863678201967557	[595]	[338,380,691,202]	-8	hSpCas9	negative selection	6761080
53647520	53647543	X	+	27260156	HCC44	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.410278943399396	[131]	[12,79,3,2]	-9	hSpCas9	negative selection	6761081
53654065	53654088	X	-	27260156	HCC44	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.5613395472949176	[737]	[314,188,358,294]	-9	hSpCas9	negative selection	6761082
53648272	53648295	X	+	27260156	HCC44	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.35709450145528143	[346]	[502,405,487,616]	6	hSpCas9	negative selection	6825338
53648224	53648247	X	-	27260156	HCC44	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-2.4976971116407456	[614]	[116,134,134,104]	-9	hSpCas9	negative selection	6825339
53654093	53654116	X	-	27260156	HCC44	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.2970244095764873	[420]	[502,265,372,428]	-5	hSpCas9	negative selection	6825340
53647531	53647554	X	-	27260156	G402	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.700234490865483	[595]	[218,305,140,231]	-9	hSpCas9	negative selection	6882331
53647520	53647543	X	+	27260156	G402	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-3.660060364862126	[131]	[26,2,0,20]	-9	hSpCas9	negative selection	6882332
53654065	53654088	X	-	27260156	G402	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.6191181495579055	[737]	[247,164,419,322]	-9	hSpCas9	negative selection	6882333
53648272	53648295	X	+	27260156	G402	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.2325781242150505	[346]	[302,446,561,637]	3	hSpCas9	negative selection	6946589
53648224	53648247	X	-	27260156	G402	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-2.7066559207007224	[614]	[142,69,136,110]	-9	hSpCas9	negative selection	6946590
53654093	53654116	X	-	27260156	G402	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.10243985729439029	[420]	[920,584,451,312]	1	hSpCas9	negative selection	6946591
53647531	53647554	X	-	27260156	L33	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	0.21995556202788163	[595]	[699,271,452,691]	5	hSpCas9	negative selection	7003582
53647520	53647543	X	+	27260156	L33	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.3408194604633945	[131]	[83,44,64,119]	-7	hSpCas9	negative selection	7003583
53654065	53654088	X	-	27260156	L33	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.063734806615334	[737]	[344,128,256,343]	-9	hSpCas9	negative selection	7003584
53648272	53648295	X	+	27260156	L33	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.21257511762537848	[346]	[413,147,296,360]	4	hSpCas9	negative selection	7067840
53648224	53648247	X	-	27260156	L33	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.6314133705305671	[614]	[386,151,332,301]	-9	hSpCas9	negative selection	7067841
53654093	53654116	X	-	27260156	L33	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.3995774819464138	[420]	[358,124,207,280]	-7	hSpCas9	negative selection	7067842
53647531	53647554	X	-	27260156	K562	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.7286410792596443	[595]	[276,390,300,307]	-7	hSpCas9	negative selection	7124833
53647520	53647543	X	+	27260156	K562	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.4012147741562213	[131]	[9,45,11,20]	-9	hSpCas9	negative selection	7124834
53654065	53654088	X	-	27260156	K562	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.3845969673223264	[737]	[272,378,156,206]	-8	hSpCas9	negative selection	7124835
53648272	53648295	X	+	27260156	K562	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.16906851660959632	[346]	[515,366,287,239]	2	hSpCas9	negative selection	7189091
53648224	53648247	X	-	27260156	K562	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.1693386823903325	[614]	[133,153,471,200]	-8	hSpCas9	negative selection	7189092
53654093	53654116	X	-	27260156	K562	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.05457530946475542	[420]	[265,576,170,419]	0	hSpCas9	negative selection	7189093
53647531	53647554	X	-	27260156	HT29	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.9130005734823534	[595]	[340,478,434,296]	-8	hSpCas9	negative selection	7246084
53647520	53647543	X	+	27260156	HT29	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.7550040974109604	[131]	[64,28,33,59]	-9	hSpCas9	negative selection	7246085
53654065	53654088	X	-	27260156	HT29	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.6116736775412104	[737]	[552,534,499,730]	-7	hSpCas9	negative selection	7246086
53648272	53648295	X	+	27260156	HT29	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.4822080100153172	[346]	[523,807,701,360]	8	hSpCas9	negative selection	7310342
53648224	53648247	X	-	27260156	HT29	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.567116667695276	[614]	[221,355,255,187]	-9	hSpCas9	negative selection	7310343
53654093	53654116	X	-	27260156	HT29	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.03212806665576978	[420]	[591,632,444,443]	0	hSpCas9	negative selection	7310344
53647531	53647554	X	-	27260156	MHHES1	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.8879935930507528	[595]	[256,408,503,283]	-9	hSpCas9	negative selection	7367335
53647520	53647543	X	+	27260156	MHHES1	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.3474259348977513	[131]	[134,171,32,128]	-6	hSpCas9	negative selection	7367336
53654065	53654088	X	-	27260156	MHHES1	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.5762626303101029	[737]	[505,704,643,406]	-8	hSpCas9	negative selection	7367337
53648272	53648295	X	+	27260156	MHHES1	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.40464671317810036	[346]	[513,672,540,377]	7	hSpCas9	negative selection	7431593
53648224	53648247	X	-	27260156	MHHES1	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.1243084601290683	[614]	[448,328,291,230]	-9	hSpCas9	negative selection	7431594
53654093	53654116	X	-	27260156	MHHES1	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.5391160720546595	[420]	[371,353,351,246]	-7	hSpCas9	negative selection	7431595
53647531	53647554	X	-	27260156	MEWO	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.33715215299594636	[595]	[442,316,564]	-6	hSpCas9	negative selection	7488586
53647520	53647543	X	+	27260156	MEWO	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.4087476673822061	[131]	[32,27,75]	-9	hSpCas9	negative selection	7488587
53654065	53654088	X	-	27260156	MEWO	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.8518011565308559	[737]	[526,301,346]	-9	hSpCas9	negative selection	7488588
53648272	53648295	X	+	27260156	MEWO	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.11348259759143525	[346]	[379,230,304]	-2	hSpCas9	negative selection	7552844
53648224	53648247	X	-	27260156	MEWO	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.0876664781357492	[614]	[294,232,288]	-9	hSpCas9	negative selection	7552845
53654093	53654116	X	-	27260156	MEWO	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.397762882350671	[420]	[349,238,317]	-7	hSpCas9	negative selection	7552846
53647531	53647554	X	-	27260156	LNCAPCLONEFGC	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.8621961464447347	[595]	[221,186,535,286]	-8	hSpCas9	negative selection	7609837
53647520	53647543	X	+	27260156	LNCAPCLONEFGC	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.9510897460321412	[131]	[54,22,25,18]	-9	hSpCas9	negative selection	7609838
53654065	53654088	X	-	27260156	LNCAPCLONEFGC	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.4976515965106318	[737]	[224,233,241,246]	-9	hSpCas9	negative selection	7609839
53648272	53648295	X	+	27260156	LNCAPCLONEFGC	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.1006574506887915	[346]	[318,327,303,392]	1	hSpCas9	negative selection	7674095
53648224	53648247	X	-	27260156	LNCAPCLONEFGC	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.3901156434574977	[614]	[239,205,232,173]	-9	hSpCas9	negative selection	7674096
53654093	53654116	X	-	27260156	LNCAPCLONEFGC	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.4260731742647175	[420]	[251,307,423,170]	-6	hSpCas9	negative selection	7674097
53647531	53647554	X	-	27260156	PANC0327	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	0.31840431339849656	[595]	[665,471,1026,500]	5	hSpCas9	negative selection	7731088
53647520	53647543	X	+	27260156	PANC0327	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.053619832764064	[131]	[101,38,79,10]	-8	hSpCas9	negative selection	7731089
53654065	53654088	X	-	27260156	PANC0327	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.2127184600420913	[737]	[301,328,294,200]	-9	hSpCas9	negative selection	7731090
53648272	53648295	X	+	27260156	PANC0327	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.16609168624830728	[346]	[477,276,319,327]	2	hSpCas9	negative selection	7795346
53648224	53648247	X	-	27260156	PANC0327	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.2626969647126671	[614]	[189,246,198,268]	-9	hSpCas9	negative selection	7795347
53654093	53654116	X	-	27260156	PANC0327	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.020948034822506595	[420]	[352,258,400,517]	0	hSpCas9	negative selection	7795348
53647531	53647554	X	-	27260156	NCIH2009	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.4155644264099023	[595]	[444,266,250]	-9	hSpCas9	negative selection	7852339
53647520	53647543	X	+	27260156	NCIH2009	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.7064726949462143	[131]	[38,109,15]	-9	hSpCas9	negative selection	7852340
53654065	53654088	X	-	27260156	NCIH2009	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.6138073964590802	[737]	[433,298,296]	-9	hSpCas9	negative selection	7852341
53648272	53648295	X	+	27260156	NCIH2009	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.5424023543553322	[346]	[739,636,747]	7	hSpCas9	negative selection	7916597
53648224	53648247	X	-	27260156	NCIH2009	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-2.2791523676590026	[614]	[214,191,130]	-9	hSpCas9	negative selection	7916598
53654093	53654116	X	-	27260156	NCIH2009	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.5643377731162393	[420]	[490,402,316]	-6	hSpCas9	negative selection	7916599
53647531	53647554	X	-	27260156	NCIH1373	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.1178392433069662	[595]	[323,298,343,68]	-8	hSpCas9	negative selection	7973590
53647520	53647543	X	+	27260156	NCIH1373	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.9889609491898703	[131]	[6,40,13,1]	-9	hSpCas9	negative selection	7973591
53654065	53654088	X	-	27260156	NCIH1373	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.0585184625542148	[737]	[675,287,301,98]	-8	hSpCas9	negative selection	7973592
53648272	53648295	X	+	27260156	NCIH1373	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.28860583365965	[346]	[523,435,508,114]	3	hSpCas9	negative selection	8037848
53648224	53648247	X	-	27260156	NCIH1373	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.7637216741880457	[614]	[151,321,115,52]	-9	hSpCas9	negative selection	8037849
53654093	53654116	X	-	27260156	NCIH1373	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.5309337264018728	[420]	[436,205,272,111]	-5	hSpCas9	negative selection	8037850
53647531	53647554	X	-	27260156	PATU8902	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-2.936494985659457	[595]	[189,29,144,49]	-9	hSpCas9	negative selection	8094841
53647520	53647543	X	+	27260156	PATU8902	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-5.255859996550176	[131]	[2,9,1,4]	-9	hSpCas9	negative selection	8094842
53654065	53654088	X	-	27260156	PATU8902	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.8277368292076952	[737]	[334,481,173,181]	-9	hSpCas9	negative selection	8094843
53648272	53648295	X	+	27260156	PATU8902	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	1.204000898986526	[346]	[1291,1357,553,1420]	9	hSpCas9	negative selection	8159099
53648224	53648247	X	-	27260156	PATU8902	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-3.0313951649159496	[614]	[116,69,108,122]	-9	hSpCas9	negative selection	8159100
53654093	53654116	X	-	27260156	PATU8902	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.2756299930118012	[420]	[382,843,427,269]	-3	hSpCas9	negative selection	8159101
53647531	53647554	X	-	27260156	PANC1	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.213032958704707	[595]	[195,410,135,181]	-9	hSpCas9	negative selection	8216092
53647520	53647543	X	+	27260156	PANC1	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.8192299600683184	[131]	[20,39,30,36]	-9	hSpCas9	negative selection	8216093
53654065	53654088	X	-	27260156	PANC1	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.9967203735306588	[737]	[338,521,230,228]	-8	hSpCas9	negative selection	8216094
53648272	53648295	X	+	27260156	PANC1	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.5096851293508282	[346]	[340,827,384,249]	7	hSpCas9	negative selection	8280350
53648224	53648247	X	-	27260156	PANC1	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.1355808538673091	[614]	[301,212,245,196]	-8	hSpCas9	negative selection	8280351
53654093	53654116	X	-	27260156	PANC1	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.3347959186312526	[420]	[372,481,498,467]	5	hSpCas9	negative selection	8280352
53647531	53647554	X	-	27260156	PANC0813	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.1513233561556768	[595]	[500,212,296,268]	-9	hSpCas9	negative selection	8337343
53647520	53647543	X	+	27260156	PANC0813	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.0781638555224236	[131]	[12,52,30,46]	-9	hSpCas9	negative selection	8337344
53654065	53654088	X	-	27260156	PANC0813	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.2676552335684392	[737]	[437,313,253,435]	-9	hSpCas9	negative selection	8337345
53648272	53648295	X	+	27260156	PANC0813	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.4008481612149418	[346]	[551,642,584,381]	6	hSpCas9	negative selection	8401601
53648224	53648247	X	-	27260156	PANC0813	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.4417076060341532	[614]	[462,163,132,317]	-9	hSpCas9	negative selection	8401602
53654093	53654116	X	-	27260156	PANC0813	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.42702751329087835	[420]	[328,300,561,280]	-6	hSpCas9	negative selection	8401603
53647531	53647554	X	-	27260156	RDES	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.8088132076648049	[595]	[283,487,399,395]	-8	hSpCas9	negative selection	8458594
53647520	53647543	X	+	27260156	RDES	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.20784294277675	[131]	[49,32,82,108]	-9	hSpCas9	negative selection	8458595
53654065	53654088	X	-	27260156	RDES	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.167086267400974	[737]	[367,424,256,452]	-9	hSpCas9	negative selection	8458596
53648272	53648295	X	+	27260156	RDES	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.505694727056365	[346]	[435,523,575,778]	7	hSpCas9	negative selection	8522852
53648224	53648247	X	-	27260156	RDES	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.7352884942824731	[614]	[213,261,972,304]	-8	hSpCas9	negative selection	8522853
53654093	53654116	X	-	27260156	RDES	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.34093801732270634	[420]	[296,357,367,538]	-5	hSpCas9	negative selection	8522854
53647531	53647554	X	-	27260156	PC3	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	0.10564887778837795	[595]	[458,595,842,753]	2	hSpCas9	negative selection	8579845
53647520	53647543	X	+	27260156	PC3	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.2117632995696026	[131]	[31,189,179,102]	-5	hSpCas9	negative selection	8579846
53654065	53654088	X	-	27260156	PC3	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.4975363023598291	[737]	[443,660,471,440]	-8	hSpCas9	negative selection	8579847
53648272	53648295	X	+	27260156	PC3	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.15194405385693882	[346]	[286,323,819,273]	3	hSpCas9	negative selection	8644103
53648224	53648247	X	-	27260156	PC3	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.6384359374400934	[614]	[181,393,927,340]	-9	hSpCas9	negative selection	8644104
53654093	53654116	X	-	27260156	PC3	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.7782131450924887	[420]	[182,328,234,219]	-9	hSpCas9	negative selection	8644105
53647531	53647554	X	-	27260156	PATU8988T	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.1334728066129025	[595]	[256,199,355,226]	-9	hSpCas9	negative selection	8701096
53647520	53647543	X	+	27260156	PATU8988T	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.6728258999529595	[131]	[73,8,172,53]	-8	hSpCas9	negative selection	8701097
53654065	53654088	X	-	27260156	PATU8988T	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.7998945499636183	[737]	[240,432,442,532]	-8	hSpCas9	negative selection	8701098
53648272	53648295	X	+	27260156	PATU8988T	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.3363512884187035	[346]	[289,477,527,398]	6	hSpCas9	negative selection	8765354
53648224	53648247	X	-	27260156	PATU8988T	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.047154378522468	[614]	[169,341,231,422]	-9	hSpCas9	negative selection	8765355
53654093	53654116	X	-	27260156	PATU8988T	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.21614066224462192	[420]	[444,522,406,524]	4	hSpCas9	negative selection	8765356
53647531	53647554	X	-	27260156	T47D	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.5607055818604696	[595]	[514,459,446,461]	-8	hSpCas9	negative selection	8822347
53647520	53647543	X	+	27260156	T47D	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.0992404855703837	[131]	[63,80,54,86]	-9	hSpCas9	negative selection	8822348
53654065	53654088	X	-	27260156	T47D	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.0183370547317732	[737]	[336,442,502,410]	-9	hSpCas9	negative selection	8822349
53648272	53648295	X	+	27260156	T47D	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.273382803475514	[346]	[495,440,464,558]	6	hSpCas9	negative selection	8886605
53648224	53648247	X	-	27260156	T47D	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.1362832561285456	[614]	[327,358,295,317]	-9	hSpCas9	negative selection	8886606
53654093	53654116	X	-	27260156	T47D	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.1371142237916803	[420]	[387,366,530,504]	-3	hSpCas9	negative selection	8886607
53647531	53647554	X	-	27260156	SU8686	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-1.0689138076753453	[595]	[255,250,258,380]	-8	hSpCas9	negative selection	8943598
53647520	53647543	X	+	27260156	SU8686	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.9944983835354824	[131]	[7,27,38,64]	-9	hSpCas9	negative selection	8943599
53654065	53654088	X	-	27260156	SU8686	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.3004405751979247	[737]	[267,293,335,291]	-9	hSpCas9	negative selection	8943600
53648272	53648295	X	+	27260156	SU8686	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.32402131056510886	[346]	[461,427,444,371]	5	hSpCas9	negative selection	9007856
53648224	53648247	X	-	27260156	SU8686	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-1.5533786546716826	[614]	[182,218,184,249]	-9	hSpCas9	negative selection	9007857
53654093	53654116	X	-	27260156	SU8686	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	0.05404031256045437	[420]	[382,343,397,657]	0	hSpCas9	negative selection	9007858
53647531	53647554	X	-	27260156	SKES1	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.4822907425400967	[595]	[491,426,279,500]	-8	hSpCas9	negative selection	9064849
53647520	53647543	X	+	27260156	SKES1	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.8753300794557537	[131]	[104,106,32,52]	-9	hSpCas9	negative selection	9064850
53654065	53654088	X	-	27260156	SKES1	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.8763390664168665	[737]	[449,482,355,335]	-9	hSpCas9	negative selection	9064851
53648272	53648295	X	+	27260156	SKES1	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.0919153568965348	[346]	[293,549,257,228]	-2	hSpCas9	negative selection	9129107
53648224	53648247	X	-	27260156	SKES1	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.9495849489405775	[614]	[312,364,247,343]	-9	hSpCas9	negative selection	9129108
53654093	53654116	X	-	27260156	SKES1	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.19601845875981772	[420]	[435,347,303,380]	-4	hSpCas9	negative selection	9129109
53647531	53647554	X	-	27260156	TOV112D	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.8839111198316414	[595]	[380,367,339,222]	-9	hSpCas9	negative selection	9186100
53647520	53647543	X	+	27260156	TOV112D	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.8734413677545836	[131]	[75,93,79,44]	-9	hSpCas9	negative selection	9186101
53654065	53654088	X	-	27260156	TOV112D	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.4718390976912167	[737]	[612,566,575,393]	-7	hSpCas9	negative selection	9186102
53648272	53648295	X	+	27260156	TOV112D	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	-0.15562945092448682	[346]	[304,462,310,207]	-3	hSpCas9	negative selection	9250358
53648224	53648247	X	-	27260156	TOV112D	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.7814494567890463	[614]	[244,431,391,371]	-9	hSpCas9	negative selection	9250359
53654093	53654116	X	-	27260156	TOV112D	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.10865252923255475	[420]	[345,474,423,336]	-2	hSpCas9	negative selection	9250360
53647531	53647554	X	-	27260156	TC71	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.25607119308662146	[595]	[550,522,516,549]	-6	hSpCas9	negative selection	9307351
53647520	53647543	X	+	27260156	TC71	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.6498743836049974	[131]	[151,36,76,93]	-9	hSpCas9	negative selection	9307352
53654065	53654088	X	-	27260156	TC71	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-0.49294470113557864	[737]	[414,630,582,625]	-8	hSpCas9	negative selection	9307353
53648272	53648295	X	+	27260156	TC71	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.14400996359988355	[346]	[515,344,310,476]	3	hSpCas9	negative selection	9371609
53648224	53648247	X	-	27260156	TC71	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.7344304455032965	[614]	[360,467,389,364]	-9	hSpCas9	negative selection	9371610
53654093	53654116	X	-	27260156	TC71	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.017102463157394276	[420]	[469,391,477,441]	0	hSpCas9	negative selection	9371611
53647531	53647554	X	-	27260156	TC32	viability	GACCTGTTGGACCGCTTCGATGG	HUWE1	ENSG00000086758	-0.4890985295226409	[595]	[301,403,425,456]	-7	hSpCas9	negative selection	9428602
53647520	53647543	X	+	27260156	TC32	viability	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.5821131341196761	[131]	[109,4,163,88]	-8	hSpCas9	negative selection	9428603
53654065	53654088	X	-	27260156	TC32	viability	ACTGAAGAAGACACCTACTGAGG	HUWE1	ENSG00000086758	-1.009159478874212	[737]	[431,227,365,368]	-9	hSpCas9	negative selection	9428604
53648272	53648295	X	+	27260156	TC32	viability	AACTTTGAGTTTGTCTATTAAGG	HUWE1	ENSG00000086758	0.36369650818413995	[346]	[355,327,559,479]	6	hSpCas9	negative selection	9492860
53648224	53648247	X	-	27260156	TC32	viability	AACTGCAGCAGATCAAAACATGG	HUWE1	ENSG00000086758	-0.771849261072343	[614]	[306,269,427,375]	-9	hSpCas9	negative selection	9492861
53654093	53654116	X	-	27260156	TC32	viability	TTGAAATCATGAAAGTAGACAGG	HUWE1	ENSG00000086758	-0.06091378474444814	[420]	[366,358,422,358]	-1	hSpCas9	negative selection	9492862
53627795	53627818	X	-	27869803	HPAFII	viability after 27 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-1.1569238783569025	[154]	[71,6,NaN]	-7	hSpCas9	negative selection	9609156
53628540	53628563	X	+	27869803	HPAFII	viability after 27 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	0.6196086435280476	[165]	[156,114,NaN]	5	hSpCas9	negative selection	9609157
53628796	53628819	X	-	27869803	HPAFII	viability after 27 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-2.967066545478068	[95]	[12,0,NaN]	-9	hSpCas9	negative selection	9609158
53631413	53631436	X	-	27869803	HPAFII	viability after 27 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	0.10066545627326562	[561]	[384,259,NaN]	0	hSpCas9	negative selection	9609159
53645323	53645346	X	+	27869803	HPAFII	viability after 27 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	0.6291104389891555	[591]	[560,414,NaN]	5	hSpCas9	negative selection	9609160
53647520	53647543	X	+	27869803	HPAFII	viability after 27 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.39723014904478	[1001]	[177,34,NaN]	-8	hSpCas9	negative selection	9609161
53627795	53627818	X	-	27869803	HPAFII	viability after 15 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-0.8098943217484209	[154]	[160,53,NaN]	-7	hSpCas9	negative selection	9691605
53628540	53628563	X	+	27869803	HPAFII	viability after 15 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	1.055746286895851	[165]	[553,256,NaN]	9	hSpCas9	negative selection	9691606
53628796	53628819	X	-	27869803	HPAFII	viability after 15 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-0.4771774466791725	[95]	[91,62,NaN]	-5	hSpCas9	negative selection	9691607
53631413	53631436	X	-	27869803	HPAFII	viability after 15 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.12405123916236782	[561]	[901,337,NaN]	-1	hSpCas9	negative selection	9691608
53645323	53645346	X	+	27869803	HPAFII	viability after 15 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-0.03249297016091579	[591]	[663,595,NaN]	0	hSpCas9	negative selection	9691609
53647520	53647543	X	+	27869803	HPAFII	viability after 15 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-1.1714543549223713	[1001]	[540,438,NaN]	-8	hSpCas9	negative selection	9691610
53627795	53627818	X	-	27869803	ASPC1	viability after 36 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-0.9546645247835028	[289]	[40,125,0]	-7	hSpCas9	negative selection	9774054
53628540	53628563	X	+	27869803	ASPC1	viability after 36 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	0.7533740871814942	[393]	[441,222,88]	7	hSpCas9	negative selection	9774055
53628796	53628819	X	-	27869803	ASPC1	viability after 36 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-0.6802161404624261	[200]	[0,47,69]	-6	hSpCas9	negative selection	9774056
53631413	53631436	X	-	27869803	ASPC1	viability after 36 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-0.4552139227067449	[1451]	[224,354,478]	-4	hSpCas9	negative selection	9774057
53645323	53645346	X	+	27869803	ASPC1	viability after 36 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.5928180811060049	[1252]	[34,102,253]	-8	hSpCas9	negative selection	9774058
53647520	53647543	X	+	27869803	ASPC1	viability after 36 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-0.3180237401490347	[2180]	[576,558,668]	-3	hSpCas9	negative selection	9774059
53627795	53627818	X	-	27869803	PATU8988S	viability after 35 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-2.00607227372297	[308]	[22,38,NaN]	-9	hSpCas9	negative selection	9856503
53628540	53628563	X	+	27869803	PATU8988S	viability after 35 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-2.510294021478849	[481]	[65,1,NaN]	-9	hSpCas9	negative selection	9856504
53628796	53628819	X	-	27869803	PATU8988S	viability after 35 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-4.6989191486156345	[160]	[0,3,NaN]	-9	hSpCas9	negative selection	9856505
53631413	53631436	X	-	27869803	PATU8988S	viability after 35 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-2.0408538179919833	[1423]	[84,193,NaN]	-9	hSpCas9	negative selection	9856506
53645323	53645346	X	+	27869803	PATU8988S	viability after 35 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.8678184622304608	[1218]	[140,127,NaN]	-9	hSpCas9	negative selection	9856507
53647520	53647543	X	+	27869803	PATU8988S	viability after 35 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-3.5717730581552636	[1873]	[51,74,NaN]	-9	hSpCas9	negative selection	9856508
53627795	53627818	X	-	27869803	PATU8988S	viability after 31 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-1.8972350970787921	[308]	[18,50,NaN]	-9	hSpCas9	negative selection	9938952
53628540	53628563	X	+	27869803	PATU8988S	viability after 31 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	-2.3158593020998537	[481]	[42,32,NaN]	-9	hSpCas9	negative selection	9938953
53628796	53628819	X	-	27869803	PATU8988S	viability after 31 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-2.954613482237912	[160]	[13,0,NaN]	-9	hSpCas9	negative selection	9938954
53631413	53631436	X	-	27869803	PATU8988S	viability after 31 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	-2.1137221226587264	[1423]	[95,175,NaN]	-9	hSpCas9	negative selection	9938955
53645323	53645346	X	+	27869803	PATU8988S	viability after 31 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	-1.366759387814395	[1218]	[180,197,NaN]	-8	hSpCas9	negative selection	9938956
53647520	53647543	X	+	27869803	PATU8988S	viability after 31 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.649576006663146	[1873]	[148,80,NaN]	-9	hSpCas9	negative selection	9938957
53627795	53627818	X	-	27869803	HPAFII	viability after 35 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	0.2143852839535989	[154]	[170,0,NaN]	1	hSpCas9	negative selection	10021401
53628540	53628563	X	+	27869803	HPAFII	viability after 35 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	0.4120091056943467	[165]	[92,132,NaN]	2	hSpCas9	negative selection	10021402
53628796	53628819	X	-	27869803	HPAFII	viability after 35 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-2.5299072879664974	[95]	[14,0,NaN]	-8	hSpCas9	negative selection	10021403
53631413	53631436	X	-	27869803	HPAFII	viability after 35 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	0.07111073001026891	[561]	[304,291,NaN]	0	hSpCas9	negative selection	10021404
53645323	53645346	X	+	27869803	HPAFII	viability after 35 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	1.1326103938944394	[591]	[745,556,NaN]	7	hSpCas9	negative selection	10021405
53647520	53647543	X	+	27869803	HPAFII	viability after 35 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.3766476592652057	[1001]	[155,31,NaN]	-8	hSpCas9	negative selection	10021406
53627795	53627818	X	-	27869803	HPAFII	viability after 31 days	GGTTGACCTTATCACCAACCTGG	HUWE1	ENSG00000086758	-0.30349422224382294	[154]	[125,9,NaN]	-2	hSpCas9	negative selection	10103850
53628540	53628563	X	+	27869803	HPAFII	viability after 31 days	CAGCATCGTAGCTGGCCAGATGG	HUWE1	ENSG00000086758	0.45696249062762656	[165]	[113,142,NaN]	3	hSpCas9	negative selection	10103851
53628796	53628819	X	-	27869803	HPAFII	viability after 31 days	ACTGGAACTGCCTCCTACCATGG	HUWE1	ENSG00000086758	-1.887935981825051	[95]	[26,0,NaN]	-8	hSpCas9	negative selection	10103852
53631413	53631436	X	-	27869803	HPAFII	viability after 31 days	CAGCATTCCTAAGGATAAGCAGG	HUWE1	ENSG00000086758	0.001779608758048834	[561]	[299,332,NaN]	0	hSpCas9	negative selection	10103853
53645323	53645346	X	+	27869803	HPAFII	viability after 31 days	TTGTAGCCGAGTTAGCAGCGGGG	HUWE1	ENSG00000086758	0.6571244937713336	[591]	[577,464,NaN]	5	hSpCas9	negative selection	10103854
53647520	53647543	X	+	27869803	HPAFII	viability after 31 days	CTGCCAGTATTCCATCGAAGCGG	HUWE1	ENSG00000086758	-2.2489022239518217	[1001]	[180,49,NaN]	-8	hSpCas9	negative selection	10103855
53604638	53604661	X	+	28162770	P31FUJ	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.6047709818013367	[87]	[33]	-7	hSpCas9	negative selection	10172174
53536626	53536649	X	-	28162770	P31FUJ	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-2.8510408600098334	[306]	[49]	-9	hSpCas9	negative selection	10172175
53592463	53592486	X	-	28162770	P31FUJ	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-3.9666280910500173	[305]	[22]	-9	hSpCas9	negative selection	10172176
53580964	53580987	X	+	28162770	P31FUJ	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-1.6492262439641852	[346]	[129]	-8	hSpCas9	negative selection	10172177
53569735	53569758	X	+	28162770	P31FUJ	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.9022609792223082	[333]	[104]	-8	hSpCas9	negative selection	10172178
53562140	53562163	X	-	28162770	P31FUJ	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-1.155761168650551	[236]	[124]	-7	hSpCas9	negative selection	10172179
53550911	53550934	X	+	28162770	P31FUJ	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-2.351196905216609	[164]	[37]	-8	hSpCas9	negative selection	10172180
53547728	53547751	X	+	28162770	P31FUJ	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-1.046033692808652	[122]	[69]	-6	hSpCas9	negative selection	10172181
53546787	53546810	X	-	28162770	P31FUJ	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.1218658030510227	[562]	[303]	-7	hSpCas9	negative selection	10172182
53546519	53546542	X	-	28162770	P31FUJ	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-4.6922338230516765	[43]	[1]	-9	hSpCas9	negative selection	10172183
53604638	53604661	X	+	28162770	TF1	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	NaN	[NaN]	[60]	1	hSpCas9	negative selection	10341540
53536626	53536649	X	-	28162770	TF1	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	NaN	[NaN]	[21]	1	hSpCas9	negative selection	10341541
53592463	53592486	X	-	28162770	TF1	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	NaN	[NaN]	[61]	1	hSpCas9	negative selection	10341542
53580964	53580987	X	+	28162770	TF1	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	NaN	[NaN]	[119]	1	hSpCas9	negative selection	10341543
53569735	53569758	X	+	28162770	TF1	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	NaN	[NaN]	[125]	1	hSpCas9	negative selection	10341544
53562140	53562163	X	-	28162770	TF1	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	NaN	[NaN]	[102]	1	hSpCas9	negative selection	10341545
53550911	53550934	X	+	28162770	TF1	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	NaN	[NaN]	[90]	1	hSpCas9	negative selection	10341546
53547728	53547751	X	+	28162770	TF1	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	NaN	[NaN]	[48]	1	hSpCas9	negative selection	10341547
53546787	53546810	X	-	28162770	TF1	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	NaN	[NaN]	[52]	1	hSpCas9	negative selection	10341548
53546519	53546542	X	-	28162770	TF1	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	NaN	[NaN]	[20]	1	hSpCas9	negative selection	10341549
53604638	53604661	X	+	28162770	SKM1	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-0.3783950465463908	[24]	[33]	-3	hSpCas9	negative selection	10510906
53536626	53536649	X	-	28162770	SKM1	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-1.6964708159381463	[54]	[29]	-8	hSpCas9	negative selection	10510907
53592463	53592486	X	-	28162770	SKM1	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-0.7103563421930964	[86]	[93]	-5	hSpCas9	negative selection	10510908
53580964	53580987	X	+	28162770	SKM1	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	0.6147308723561573	[98]	[267]	6	hSpCas9	negative selection	10510909
53569735	53569758	X	+	28162770	SKM1	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	0.01706208376293672	[89]	[160]	0	hSpCas9	negative selection	10510910
53562140	53562163	X	-	28162770	SKM1	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	0.11659775731385119	[71]	[137]	1	hSpCas9	negative selection	10510911
53550911	53550934	X	+	28162770	SKM1	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-0.147771859203762	[46]	[74]	-1	hSpCas9	negative selection	10510912
53547728	53547751	X	+	28162770	SKM1	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	0.788051783661981	[37]	[115]	7	hSpCas9	negative selection	10510913
53546787	53546810	X	-	28162770	SKM1	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-0.12721591934809806	[117]	[190]	-1	hSpCas9	negative selection	10510914
53546519	53546542	X	-	28162770	SKM1	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-3.8220016980220053	[7]	[0]	-9	hSpCas9	negative selection	10510915
53604638	53604661	X	+	28162770	SKM1	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-0.3783950465463908	[24]	[33]	-3	hSpCas9	negative selection	10680272
53536626	53536649	X	-	28162770	SKM1	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-1.6964708159381463	[54]	[29]	-8	hSpCas9	negative selection	10680273
53592463	53592486	X	-	28162770	SKM1	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-0.7103563421930964	[86]	[93]	-5	hSpCas9	negative selection	10680274
53580964	53580987	X	+	28162770	SKM1	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	0.6147308723561573	[98]	[267]	6	hSpCas9	negative selection	10680275
53569735	53569758	X	+	28162770	SKM1	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	0.01706208376293672	[89]	[160]	0	hSpCas9	negative selection	10680276
53562140	53562163	X	-	28162770	SKM1	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	0.11659775731385119	[71]	[137]	1	hSpCas9	negative selection	10680277
53550911	53550934	X	+	28162770	SKM1	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-0.147771859203762	[46]	[74]	-1	hSpCas9	negative selection	10680278
53547728	53547751	X	+	28162770	SKM1	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	0.788051783661981	[37]	[115]	7	hSpCas9	negative selection	10680279
53546787	53546810	X	-	28162770	SKM1	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-0.12721591934809806	[117]	[190]	-1	hSpCas9	negative selection	10680280
53546519	53546542	X	-	28162770	SKM1	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-3.8220016980220053	[7]	[0]	-9	hSpCas9	negative selection	10680281
53604638	53604661	X	+	28162770	PL21	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.763334509485566	[77]	[9]	-8	hSpCas9	negative selection	10849638
53536626	53536649	X	-	28162770	PL21	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-0.427891608123722	[169]	[54]	-3	hSpCas9	negative selection	10849639
53592463	53592486	X	-	28162770	PL21	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-1.6352296837378808	[363]	[50]	-8	hSpCas9	negative selection	10849640
53580964	53580987	X	+	28162770	PL21	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-0.9390231331823052	[347]	[78]	-6	hSpCas9	negative selection	10849641
53569735	53569758	X	+	28162770	PL21	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	0.5465900285107513	[279]	[177]	5	hSpCas9	negative selection	10849642
53562140	53562163	X	-	28162770	PL21	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	0.22540637309974998	[282]	[143]	2	hSpCas9	negative selection	10849643
53550911	53550934	X	+	28162770	PL21	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-0.8346258036713565	[167]	[40]	-5	hSpCas9	negative selection	10849644
53547728	53547751	X	+	28162770	PL21	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	2.035752796588438	[64]	[115]	9	hSpCas9	negative selection	10849645
53546787	53546810	X	-	28162770	PL21	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-0.11152014494421503	[349]	[140]	-1	hSpCas9	negative selection	10849646
53546519	53546542	X	-	28162770	PL21	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-2.641162639491622	[42]	[2]	-9	hSpCas9	negative selection	10849647
53604638	53604661	X	+	28162770	P31FUJ	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.6047709818013367	[87]	[33]	-7	hSpCas9	negative selection	11019004
53536626	53536649	X	-	28162770	P31FUJ	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-2.8510408600098334	[306]	[49]	-9	hSpCas9	negative selection	11019005
53592463	53592486	X	-	28162770	P31FUJ	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-3.9666280910500173	[305]	[22]	-9	hSpCas9	negative selection	11019006
53580964	53580987	X	+	28162770	P31FUJ	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-1.6492262439641852	[346]	[129]	-8	hSpCas9	negative selection	11019007
53569735	53569758	X	+	28162770	P31FUJ	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.9022609792223082	[333]	[104]	-8	hSpCas9	negative selection	11019008
53562140	53562163	X	-	28162770	P31FUJ	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-1.155761168650551	[236]	[124]	-7	hSpCas9	negative selection	11019009
53550911	53550934	X	+	28162770	P31FUJ	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-2.351196905216609	[164]	[37]	-8	hSpCas9	negative selection	11019010
53547728	53547751	X	+	28162770	P31FUJ	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-1.046033692808652	[122]	[69]	-6	hSpCas9	negative selection	11019011
53546787	53546810	X	-	28162770	P31FUJ	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.1218658030510227	[562]	[303]	-7	hSpCas9	negative selection	11019012
53546519	53546542	X	-	28162770	P31FUJ	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-4.6922338230516765	[43]	[1]	-9	hSpCas9	negative selection	11019013
53604638	53604661	X	+	28162770	OCIAML5	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-0.6176013172269743	[33]	[39]	-4	hSpCas9	negative selection	11188370
53536626	53536649	X	-	28162770	OCIAML5	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-1.8196450931716195	[175]	[89]	-7	hSpCas9	negative selection	11188371
53592463	53592486	X	-	28162770	OCIAML5	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.4703876685315844	[174]	[56]	-8	hSpCas9	negative selection	11188372
53580964	53580987	X	+	28162770	OCIAML5	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-1.2590667510774223	[178]	[134]	-6	hSpCas9	negative selection	11188373
53569735	53569758	X	+	28162770	OCIAML5	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.060059613139829	[215]	[186]	-6	hSpCas9	negative selection	11188374
53562140	53562163	X	-	28162770	OCIAML5	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-0.010131417096248263	[158]	[284]	0	hSpCas9	negative selection	11188375
53550911	53550934	X	+	28162770	OCIAML5	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-1.2861624059585255	[126]	[93]	-6	hSpCas9	negative selection	11188376
53547728	53547751	X	+	28162770	OCIAML5	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-0.7410352584752531	[49]	[53]	-5	hSpCas9	negative selection	11188377
53546787	53546810	X	-	28162770	OCIAML5	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.507418399476551	[251]	[159]	-7	hSpCas9	negative selection	11188378
53546519	53546542	X	-	28162770	OCIAML5	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-2.489496491479289	[27]	[8]	-8	hSpCas9	negative selection	11188379
53604638	53604661	X	+	28162770	EOL1	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-0.6238888531255076	[116]	[39]	-4	hSpCas9	negative selection	11357736
53536626	53536649	X	-	28162770	EOL1	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-1.5183957242781938	[260]	[47]	-7	hSpCas9	negative selection	11357737
53592463	53592486	X	-	28162770	EOL1	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-0.7110408022205896	[521]	[167]	-5	hSpCas9	negative selection	11357738
53580964	53580987	X	+	28162770	EOL1	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-1.1982121217930977	[661]	[151]	-7	hSpCas9	negative selection	11357739
53569735	53569758	X	+	28162770	EOL1	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.0870402027046775	[624]	[154]	-6	hSpCas9	negative selection	11357740
53562140	53562163	X	-	28162770	EOL1	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-0.5815339403655843	[604]	[212]	-4	hSpCas9	negative selection	11357741
53550911	53550934	X	+	28162770	EOL1	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-1.540833113397949	[242]	[43]	-7	hSpCas9	negative selection	11357742
53547728	53547751	X	+	28162770	EOL1	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	0.16555587107432923	[175]	[103]	1	hSpCas9	negative selection	11357743
53546787	53546810	X	-	28162770	EOL1	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.582712372157378	[505]	[88]	-7	hSpCas9	negative selection	11357744
53546519	53546542	X	-	28162770	EOL1	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-3.830339730592934	[26]	[0]	-9	hSpCas9	negative selection	11357745
53604638	53604661	X	+	28162770	MOLM13	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.9471481177342653	[95]	[17]	-7	hSpCas9	negative selection	11527102
53536626	53536649	X	-	28162770	MOLM13	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-2.8419658810422086	[237]	[23]	-8	hSpCas9	negative selection	11527103
53592463	53592486	X	-	28162770	MOLM13	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-4.270202878075912	[426]	[15]	-9	hSpCas9	negative selection	11527104
53580964	53580987	X	+	28162770	MOLM13	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	0.30427815403446856	[512]	[457]	2	hSpCas9	negative selection	11527105
53569735	53569758	X	+	28162770	MOLM13	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-0.7804982483248134	[391]	[164]	-4	hSpCas9	negative selection	11527106
53562140	53562163	X	-	28162770	MOLM13	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	0.26261549377399707	[376]	[326]	2	hSpCas9	negative selection	11527107
53550911	53550934	X	+	28162770	MOLM13	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-0.34980748473448436	[244]	[138]	-2	hSpCas9	negative selection	11527108
53547728	53547751	X	+	28162770	MOLM13	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-1.3508703037852747	[126]	[35]	-6	hSpCas9	negative selection	11527109
53546787	53546810	X	-	28162770	MOLM13	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-2.5434794325346184	[636]	[78]	-8	hSpCas9	negative selection	11527110
53546519	53546542	X	-	28162770	MOLM13	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-5.721935177335439	[72]	[0]	-9	hSpCas9	negative selection	11527111
53604638	53604661	X	+	28162770	HEL	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.2232438200279554	[112]	[30]	-6	hSpCas9	negative selection	11696468
53536626	53536649	X	-	28162770	HEL	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-1.6860429339280676	[210]	[41]	-7	hSpCas9	negative selection	11696469
53592463	53592486	X	-	28162770	HEL	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.7166639683056686	[389]	[37]	-8	hSpCas9	negative selection	11696470
53580964	53580987	X	+	28162770	HEL	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-0.4018267204330874	[525]	[254]	-3	hSpCas9	negative selection	11696471
53569735	53569758	X	+	28162770	HEL	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-0.9085405424645105	[380]	[129]	-5	hSpCas9	negative selection	11696472
53562140	53562163	X	-	28162770	HEL	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-0.20171118883225464	[324]	[180]	-1	hSpCas9	negative selection	11696473
53550911	53550934	X	+	28162770	HEL	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-1.7722986672784868	[239]	[44]	-7	hSpCas9	negative selection	11696474
53547728	53547751	X	+	28162770	HEL	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-2.43254929530388	[117]	[13]	-8	hSpCas9	negative selection	11696475
53546787	53546810	X	-	28162770	HEL	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.0892775461941158	[484]	[145]	-6	hSpCas9	negative selection	11696476
53546519	53546542	X	-	28162770	HEL	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-5.001117357774367	[49]	[0]	-9	hSpCas9	negative selection	11696477
53604638	53604661	X	+	28162770	MV411	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.8009254707786235	[90]	[21]	-8	hSpCas9	negative selection	11865834
53536626	53536649	X	-	28162770	MV411	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-0.7525624492172246	[177]	[88]	-5	hSpCas9	negative selection	11865835
53592463	53592486	X	-	28162770	MV411	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.7932044337145707	[287]	[34]	-9	hSpCas9	negative selection	11865836
53580964	53580987	X	+	28162770	MV411	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-1.6594530448257432	[344]	[91]	-7	hSpCas9	negative selection	11865837
53569735	53569758	X	+	28162770	MV411	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.433740265197684	[278]	[86]	-7	hSpCas9	negative selection	11865838
53562140	53562163	X	-	28162770	MV411	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-1.089920584674193	[234]	[92]	-6	hSpCas9	negative selection	11865839
53550911	53550934	X	+	28162770	MV411	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-1.0326703684099603	[152]	[62]	-6	hSpCas9	negative selection	11865840
53547728	53547751	X	+	28162770	MV411	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-0.243548801729367	[103]	[73]	-2	hSpCas9	negative selection	11865841
53546787	53546810	X	-	28162770	MV411	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.082805812316591	[434]	[172]	-6	hSpCas9	negative selection	11865842
53546519	53546542	X	-	28162770	MV411	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-1.6400877199588124	[36]	[9]	-7	hSpCas9	negative selection	11865843
53604638	53604661	X	+	28162770	MONOMAC1	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-0.35231507253746464	[48]	[43]	-3	hSpCas9	negative selection	12035200
53536626	53536649	X	-	28162770	MONOMAC1	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-3.444964360503139	[132]	[13]	-9	hSpCas9	negative selection	12035201
53592463	53592486	X	-	28162770	MONOMAC1	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.004391769117158	[202]	[57]	-8	hSpCas9	negative selection	12035202
53580964	53580987	X	+	28162770	MONOMAC1	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-1.1764755245772374	[208]	[105]	-7	hSpCas9	negative selection	12035203
53569735	53569758	X	+	28162770	MONOMAC1	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.4296976038498288	[187]	[79]	-8	hSpCas9	negative selection	12035204
53562140	53562163	X	-	28162770	MONOMAC1	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-0.8250680696725958	[186]	[120]	-6	hSpCas9	negative selection	12035205
53550911	53550934	X	+	28162770	MONOMAC1	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-2.3459002329740364	[101]	[22]	-9	hSpCas9	negative selection	12035206
53547728	53547751	X	+	28162770	MONOMAC1	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	0.24656980441606113	[49]	[67]	2	hSpCas9	negative selection	12035207
53546787	53546810	X	-	28162770	MONOMAC1	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.7205988031165664	[252]	[87]	-8	hSpCas9	negative selection	12035208
53546519	53546542	X	-	28162770	MONOMAC1	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-4.589354269838314	[20]	[0]	-9	hSpCas9	negative selection	12035209
53604638	53604661	X	+	28162770	OCIAML2	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-1.7087895624369254	[22]	[38]	-7	hSpCas9	negative selection	12204566
53536626	53536649	X	-	28162770	OCIAML2	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-2.011198206604864	[39]	[54]	-8	hSpCas9	negative selection	12204567
53592463	53592486	X	-	28162770	OCIAML2	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.8297109185469895	[58]	[45]	-9	hSpCas9	negative selection	12204568
53580964	53580987	X	+	28162770	OCIAML2	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	0.32947535773605474	[56]	[396]	3	hSpCas9	negative selection	12204569
53569735	53569758	X	+	28162770	OCIAML2	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.1442694840395382	[64]	[162]	-6	hSpCas9	negative selection	12204570
53562140	53562163	X	-	28162770	OCIAML2	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	0.4126739500487415	[36]	[272]	4	hSpCas9	negative selection	12204571
53550911	53550934	X	+	28162770	OCIAML2	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-2.5136985471340467	[33]	[32]	-8	hSpCas9	negative selection	12204572
53547728	53547751	X	+	28162770	OCIAML2	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	1.1901001830969598	[16]	[214]	9	hSpCas9	negative selection	12204573
53546787	53546810	X	-	28162770	OCIAML2	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-1.5014358720233356	[70]	[138]	-7	hSpCas9	negative selection	12204574
53546519	53546542	X	-	28162770	OCIAML2	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-3.6930222465786096	[6]	[2]	-9	hSpCas9	negative selection	12204575
53604638	53604661	X	+	28162770	OCIAML3	viability	GCAGTGAGTGCATGCAGCAGAGG	HUWE1	ENSG00000086758	-2.3595423866871403	[16]	[9]	-9	hSpCas9	negative selection	12373932
53536626	53536649	X	-	28162770	OCIAML3	viability	GAAGAAGGGCAGGATGCTGGTGG	HUWE1	ENSG00000086758	-2.0746334812305838	[59]	[42]	-8	hSpCas9	negative selection	12373933
53592463	53592486	X	-	28162770	OCIAML3	viability	GGAGGGGTCTCGAGGAGAAGAGG	HUWE1	ENSG00000086758	-2.104622799585238	[103]	[72]	-8	hSpCas9	negative selection	12373934
53580964	53580987	X	+	28162770	OCIAML3	viability	GCTGCCAGACACAACACCAGAGG	HUWE1	ENSG00000086758	-2.089535057463375	[85]	[60]	-8	hSpCas9	negative selection	12373935
53569735	53569758	X	+	28162770	OCIAML3	viability	GAACAGGCGGGCCAAGGCAGGGG	HUWE1	ENSG00000086758	-1.7195385224080222	[83]	[76]	-8	hSpCas9	negative selection	12373936
53562140	53562163	X	-	28162770	OCIAML3	viability	TGATAGTCAGGATGAAGAGGAGG	HUWE1	ENSG00000086758	-0.3446481713874459	[52]	[125]	-3	hSpCas9	negative selection	12373937
53550911	53550934	X	+	28162770	OCIAML3	viability	GAGAATAGCAGAGAGTGCGGAGG	HUWE1	ENSG00000086758	-1.3309732344903695	[49]	[59]	-7	hSpCas9	negative selection	12373938
53547728	53547751	X	+	28162770	OCIAML3	viability	GACAATGGTGGAAATAGCCGTGG	HUWE1	ENSG00000086758	-0.05635085438136356	[30]	[89]	0	hSpCas9	negative selection	12373939
53546787	53546810	X	-	28162770	OCIAML3	viability	GGGCAGCAAATCTCCAGCGAAGG	HUWE1	ENSG00000086758	-2.0435335159882975	[126]	[92]	-8	hSpCas9	negative selection	12373940
53546519	53546542	X	-	28162770	OCIAML3	viability	GTACTACTGCAGCTCTCCCGGGG	HUWE1	ENSG00000086758	-2.5940076403241634	[5]	[2]	-9	hSpCas9	negative selection	12373941