Crispr

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 3845
• Symbol: KRAS
• Description: KRAS proto-oncogene, GTPase

DataSource: http://genomecrispr.dkfz.de/#!/results/KRAS

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
25227295	25227318	12	-	26472758	Jiyoye	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	3.010516411541884	[12]	[78]	9	hSpCas9	negative selection	86069
25245310	25245333	12	+	26472758	Jiyoye	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	5.53645839845084	[0]	[34]	9	hSpCas9	negative selection	86070
25245341	25245364	12	-	26472758	Jiyoye	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-0.17288831980244357	[440]	[294]	-1	hSpCas9	negative selection	86071
25227320	25227343	12	-	26472758	Jiyoye	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-0.06489306280934914	[257]	[185]	0	hSpCas9	negative selection	86072
25225730	25225753	12	-	26472758	Jiyoye	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.034900201857831936	[276]	[213]	0	hSpCas9	negative selection	86073
25225670	25225693	12	-	26472758	Jiyoye	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	-0.8061291670026156	[210]	[90]	-5	hSpCas9	negative selection	86074
25227305	25227328	12	-	26472758	Jiyoye	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.6042122285654273	[40]	[46]	4	hSpCas9	negative selection	86075
25227345	25227368	12	-	26472758	Jiyoye	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	-0.40854840408888693	[388]	[220]	-3	hSpCas9	negative selection	86076
25227295	25227318	12	-	26472758	KBM7	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.8695937252097419	[613,473]	[75,194]	-6	hSpCas9	negative selection	277187
25245310	25245333	12	+	26472758	KBM7	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.6002542305077794	[352,266]	[248,191]	6	hSpCas9	negative selection	277188
25245341	25245364	12	-	26472758	KBM7	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-3.1747945044492845	[1678,1161]	[73,71]	-9	hSpCas9	negative selection	277189
25227320	25227343	12	-	26472758	KBM7	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-1.7049922630486136	[941,926]	[185,89]	-8	hSpCas9	negative selection	277190
25225730	25225753	12	-	26472758	KBM7	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-1.3636507508021125	[1059,699]	[125,186]	-7	hSpCas9	negative selection	277191
25225670	25225693	12	-	26472758	KBM7	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	-0.8985289410750164	[559,402]	[166,77]	-6	hSpCas9	negative selection	277192
25227305	25227328	12	-	26472758	KBM7	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.1215554061952717	[512,289]	[89,79]	-7	hSpCas9	negative selection	277193
25227345	25227368	12	-	26472758	KBM7	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.1570460164363483	[1164,901]	[491,577]	1	hSpCas9	negative selection	277194
25227295	25227318	12	-	26472758	Raji	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.5739834493006191	[262]	[40]	-4	hSpCas9	negative selection	468305
25245310	25245333	12	+	26472758	Raji	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	-0.8510373608750008	[170]	[21]	-6	hSpCas9	negative selection	468306
25245341	25245364	12	-	26472758	Raji	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	0.005845508911537234	[617]	[143]	0	hSpCas9	negative selection	468307
25227320	25227343	12	-	26472758	Raji	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.31063671288197625	[270]	[77]	3	hSpCas9	negative selection	468308
25225730	25225753	12	-	26472758	Raji	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.48135837803055714	[391]	[126]	4	hSpCas9	negative selection	468309
25225670	25225693	12	-	26472758	Raji	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	-0.1780186834886488	[311]	[63]	-1	hSpCas9	negative selection	468310
25227305	25227328	12	-	26472758	Raji	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.0355744184684437	[158]	[17]	-6	hSpCas9	negative selection	468311
25227345	25227368	12	-	26472758	Raji	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	1.2196497676245115	[345]	[186]	9	hSpCas9	negative selection	468312
25227295	25227318	12	-	26472758	K562	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.6573172560186872	[453]	[242]	-4	hSpCas9	negative selection	659423
25245310	25245333	12	+	26472758	K562	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.022026306329999024	[160]	[137]	0	hSpCas9	negative selection	659424
25245341	25245364	12	-	26472758	K562	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-0.6177720604712575	[1286]	[707]	-4	hSpCas9	negative selection	659425
25227320	25227343	12	-	26472758	K562	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-0.007327606417263266	[661]	[555]	0	hSpCas9	negative selection	659426
25225730	25225753	12	-	26472758	K562	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-0.07155464408329426	[543]	[436]	0	hSpCas9	negative selection	659427
25225670	25225693	12	-	26472758	K562	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.5779451479670682	[445]	[561]	5	hSpCas9	negative selection	659428
25227305	25227328	12	-	26472758	K562	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.006475115295134781	[212]	[178]	0	hSpCas9	negative selection	659429
25227345	25227368	12	-	26472758	K562	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.29090774554165244	[854]	[882]	2	hSpCas9	negative selection	659430
25220867	25220890	12	+	25494202	A375	resistance to PLX-4720 (puromycin)	ATACAAAAATCAGTCAGGTGTGG	KRAS	ENSG00000133703	0.6473066130133476	[37,123]	[42,11]	2	dCas9-VP64	positive selection	2389526
25216428	25216451	12	-	25494202	A375	resistance to PLX-4720 (puromycin)	ATACAAAAATCAGTCAGGTGTGG	KRAS	ENSG00000133703	0.6473066130133476	[37,123]	[42,11]	2	dCas9-VP64	positive selection	2389531
25249330	25249353	12	+	25494202	A375	resistance to PLX-4720 (puromycin)	GAGGTTGCAGTGAGCCGAGACGG	KRAS	ENSG00000133703	0.6330757747468561	[50,16]	[8,13]	2	dCas9-VP64	positive selection	2476958
25220867	25220890	12	+	25494202	A375	resistance to PLX-4720 (zeocin)	ATACAAAAATCAGTCAGGTGTGG	KRAS	ENSG00000133703	0.8677968216831808	[99,192]	[113,60]	6	dCas9-VP64	positive selection	2484781
25216428	25216451	12	-	25494202	A375	resistance to PLX-4720 (zeocin)	ATACAAAAATCAGTCAGGTGTGG	KRAS	ENSG00000133703	0.8677968216831808	[99,192]	[113,60]	6	dCas9-VP64	positive selection	2484786
25249330	25249353	12	+	25494202	A375	resistance to PLX-4720 (zeocin)	GAGGTTGCAGTGAGCCGAGACGG	KRAS	ENSG00000133703	0.34517561365305327	[29,16]	[7,9]	2	dCas9-VP64	positive selection	2572213
25227387	25227410	12	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	AATTACTACTTGCTTCCTGTAGG	KRAS	ENSG00000133703	-0.48544145692218366	[3,3]	[3,0]	-6	hSpCas9	positive selection	2998069
25227319	25227342	12	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	AGAGGAGTACAGTGCAATGAGGG	KRAS	ENSG00000133703	-0.38423891445620306	[3,3]	[4,0]	-5	hSpCas9	positive selection	2998070
25245273	25245296	12	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CGAATATGATCCAACAATAGAGG	KRAS	ENSG00000133703	0.8456355625647127	[3,3]	[2,8]	9	hSpCas9	positive selection	2998071
25227401	25227424	12	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TCCCTTCTCAGGATTCCTACAGG	KRAS	ENSG00000133703	0.2640035084151677	[3,3]	[3,4]	5	hSpCas9	positive selection	2998072
25227387	25227410	12	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	AATTACTACTTGCTTCCTGTAGG	KRAS	ENSG00000133703	-0.3308224085009439	[3,3]	[2,0]	-5	hSpCas9	positive selection	3065927
25227319	25227342	12	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	AGAGGAGTACAGTGCAATGAGGG	KRAS	ENSG00000133703	-0.532702922234199	[3,3]	[2,0]	-7	hSpCas9	positive selection	3065928
25245273	25245296	12	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CGAATATGATCCAACAATAGAGG	KRAS	ENSG00000133703	-0.26288269102465645	[3,3]	[3,0]	-4	hSpCas9	positive selection	3065929
25227401	25227424	12	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TCCCTTCTCAGGATTCCTACAGG	KRAS	ENSG00000133703	0.4429044156256772	[3,3]	[4,3]	7	hSpCas9	positive selection	3065930
25227305	25227328		-	24336571	A375	resistance to PLX after 7 days	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.32510746461648093	[9,7]	[10,10]	8	hSpCas9	positive selection	3123686
25227305	25227328		-	24336571	A375	resistance to PLX after 14 days	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	1.5824639687484234	[5,9]	[9,12]	9	hSpCas9	positive selection	3181479
25227305	25227328		-	24336571	A375	viability after 7 days	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.43468400032753324	[6]	[9,7]	6	hSpCas9	negative selection	3239272
25227305	25227328		-	24336571	A375	viability after 14 days	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.3004107685820525	[6]	[5,9]	4	hSpCas9	negative selection	3297065
25225720	25225743	12	-	27383988	293T	resistance to West Nile virus (flavivirus)	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	0.16986337019549572	[135,135]	[0,0]	0	hSpCas9	positive selection	3376209
25215475	25215498	12	-	27383988	293T	resistance to West Nile virus (flavivirus)	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	1.797894592808538	[43,43]	[0,0]	7	hSpCas9	positive selection	3376210
25227386	25227409	12	+	26780180	HT29	viability (Avana library 4 designs)	AATTACTACTTGCTTCCTGTAGG	KRAS	ENSG00000133703	1.71830040893906	[]	[]	6	hSpCas9	negative selection	3435521
25227319	25227342	12	-	26780180	HT29	viability (Avana library 4 designs)	AGAGGAGTACAGTGCAATGAGGG	KRAS	ENSG00000133703	1.66439377439668	[]	[]	6	hSpCas9	negative selection	3435522
25245273	25245296	12	-	26780180	HT29	viability (Avana library 4 designs)	CGAATATGATCCAACAATAGAGG	KRAS	ENSG00000133703	2.97187545772855	[]	[]	8	hSpCas9	negative selection	3435523
25227401	25227424	12	-	26780180	HT29	viability (Avana library 4 designs)	TCCCTTCTCAGGATTCCTACAGG	KRAS	ENSG00000133703	0.928184980653495	[]	[]	3	hSpCas9	negative selection	3435524
25227401	25227424	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	TCCCTTCTCAGGATTCCTACAGG	KRAS	ENSG00000133703	2.28822753636483	[]	[]	3	hSpCas9	negative selection	3523129
25227386	25227409	12	+	26780180	A375	viability (Avana lentiCRISPRv2)	AATTACTACTTGCTTCCTGTAGG	KRAS	ENSG00000133703	-9.51421387458573	[]	[]	-7	hSpCas9	negative selection	3523130
25227319	25227342	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	AGAGGAGTACAGTGCAATGAGGG	KRAS	ENSG00000133703	1.12943185576063	[]	[]	1	hSpCas9	negative selection	3523131
25245273	25245296	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	CGAATATGATCCAACAATAGAGG	KRAS	ENSG00000133703	-12.6105103147737	[]	[]	-8	hSpCas9	negative selection	3523132
25227337	25227360	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-3.58368508760022	[]	[]	-4	hSpCas9	negative selection	3523133
25227298	25227321	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	GGACCAGTACATGAGGACTGGGG	KRAS	ENSG00000133703	3.17425362768997	[]	[]	5	hSpCas9	negative selection	3523134
25227401	25227424	12	-	26780180	A375	viability (Avana lentiGuide)	TCCCTTCTCAGGATTCCTACAGG	KRAS	ENSG00000133703	-2.50081258974686	[]	[]	-4	hSpCas9	negative selection	3631788
25227386	25227409	12	+	26780180	A375	viability (Avana lentiGuide)	AATTACTACTTGCTTCCTGTAGG	KRAS	ENSG00000133703	7.6701561845403	[]	[]	6	hSpCas9	negative selection	3631789
25227319	25227342	12	-	26780180	A375	viability (Avana lentiGuide)	AGAGGAGTACAGTGCAATGAGGG	KRAS	ENSG00000133703	-0.138232151630188	[]	[]	0	hSpCas9	negative selection	3631790
25245273	25245296	12	-	26780180	A375	viability (Avana lentiGuide)	CGAATATGATCCAACAATAGAGG	KRAS	ENSG00000133703	1.06130106742864	[]	[]	1	hSpCas9	negative selection	3631791
25227337	25227360	12	-	26780180	A375	viability (Avana lentiGuide)	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	2.27118167389633	[]	[]	2	hSpCas9	negative selection	3631792
25227298	25227321	12	-	26780180	A375	viability (Avana lentiGuide)	GGACCAGTACATGAGGACTGGGG	KRAS	ENSG00000133703	14.6888538277748	[]	[]	8	hSpCas9	negative selection	3631793
25227401	25227424	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TCCCTTCTCAGGATTCCTACAGG	KRAS	ENSG00000133703	-0.0401981790141209	[5]	[6,3]	0	hSpCas9	positive selection	3740447
25227386	25227409	12	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AATTACTACTTGCTTCCTGTAGG	KRAS	ENSG00000133703	-0.6640056335556827	[6]	[4,2]	-7	hSpCas9	positive selection	3740448
25227319	25227342	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AGAGGAGTACAGTGCAATGAGGG	KRAS	ENSG00000133703	0.46932975150844003	[5]	[6,8]	9	hSpCas9	positive selection	3740449
25245273	25245296	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CGAATATGATCCAACAATAGAGG	KRAS	ENSG00000133703	-0.8875097484053248	[6]	[3,2]	-8	hSpCas9	positive selection	3740450
25227337	25227360	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.17985114426466842	[0]	[0,1]	5	hSpCas9	positive selection	3740451
25227298	25227321	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GGACCAGTACATGAGGACTGGGG	KRAS	ENSG00000133703	-0.058147811434659885	[4]	[3,4]	-1	hSpCas9	positive selection	3740452
25227401	25227424	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TCCCTTCTCAGGATTCCTACAGG	KRAS	ENSG00000133703	0.27542940040047026	[5]	[6,6]	7	hSpCas9	positive selection	3849054
25227386	25227409	12	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AATTACTACTTGCTTCCTGTAGG	KRAS	ENSG00000133703	-0.327076340397956	[5]	[3,3]	-4	hSpCas9	positive selection	3849055
25227319	25227342	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AGAGGAGTACAGTGCAATGAGGG	KRAS	ENSG00000133703	0.9302828512328604	[5]	[9,10]	9	hSpCas9	positive selection	3849056
25245273	25245296	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CGAATATGATCCAACAATAGAGG	KRAS	ENSG00000133703	-0.005849270428138126	[4]	[4,3]	0	hSpCas9	positive selection	3849057
25227337	25227360	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.4311201558111455	[1]	[0,0]	-5	hSpCas9	positive selection	3849058
25227298	25227321	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GGACCAGTACATGAGGACTGGGG	KRAS	ENSG00000133703	0.09398374063285868	[5]	[3,5]	2	hSpCas9	positive selection	3849059
25215475	25215498	12	-	26780180	A375	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	1.75016529318427	[]	[]	9	hSpCas9	negative selection	3955141
25227305	25227328	12	-	26780180	A375	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	1.05226050472708	[]	[]	5	hSpCas9	negative selection	3955142
25225720	25225743	12	-	26780180	A375	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	1.47968993739535	[]	[]	8	hSpCas9	negative selection	3955143
25227337	25227360	12	-	26780180	A375	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	1.39684138787806	[]	[]	7	hSpCas9	negative selection	3955144
25209876	25209899	12	+	26780180	A375	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.768116448428465	[]	[]	3	hSpCas9	negative selection	3955145
25215475	25215498	12	-	26780180	HT29	viability (GeCKOv2 library)	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	1.81643406472502	[]	[]	8	hSpCas9	negative selection	4063107
25227305	25227328	12	-	26780180	HT29	viability (GeCKOv2 library)	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.808255909915405	[]	[]	3	hSpCas9	negative selection	4063108
25225720	25225743	12	-	26780180	HT29	viability (GeCKOv2 library)	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	0.887341618774554	[]	[]	4	hSpCas9	negative selection	4063109
25227337	25227360	12	-	26780180	HT29	viability (GeCKOv2 library)	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.133354743668098	[]	[]	0	hSpCas9	negative selection	4063110
25209876	25209899	12	+	26780180	HT29	viability (GeCKOv2 library)	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.269414606033542	[]	[]	0	hSpCas9	negative selection	4063111
25227305	25227328	12	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.6673498989395792	[4]	[7,3]	9	hSpCas9	positive selection	4147638
25227305	25227328	12	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	1.1947968781509366	[4]	[5,3,13,1]	8	hSpCas9	positive selection	4211660
25227337	25227360	12	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	1.206069727185501	[3]	[6,5]	9	hSpCas9	positive selection	4279059
25227305	25227328	12	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.21161118400777906	[2]	[2,1]	4	hSpCas9	positive selection	4279060
25209876	25209899	12	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.03147735880835478	[2]	[3,1]	0	hSpCas9	positive selection	4279061
25225720	25225743	12	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	0.03337290488536334	[3]	[2,1]	0	hSpCas9	positive selection	4343315
25215475	25215498	12	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.5675342283112181	[2]	[1,0]	-8	hSpCas9	positive selection	4343316
25225682	25225704	12	+	27760321	OCIAML3	viability	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-3.0328564401031217	[63,84]	[11,0]	-9	hSpCas9	negative selection	4412999
25227230	25227252	12	-	27760321	OCIAML3	viability	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-3.6529962146942783	[8,21]	[0,0]	-9	hSpCas9	negative selection	4413000
25227299	25227321	12	-	27760321	OCIAML3	viability	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	-0.773735787162444	[569,439]	[270,174]	-6	hSpCas9	negative selection	4413001
25227337	25227359	12	-	27760321	OCIAML3	viability	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.28924654691123575	[366,348]	[152,328]	-3	hSpCas9	negative selection	4413002
25227354	25227376	12	+	27760321	OCIAML3	viability	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-1.1722778559177107	[130,124]	[17,78]	-8	hSpCas9	negative selection	4413003
25225682	25225704	12	+	27760321	OCIAML2	viability	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-0.38484435055676647	[63,84]	[48,43]	-4	hSpCas9	negative selection	4496462
25227230	25227252	12	-	27760321	OCIAML2	viability	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-3.701741579959327	[8,21]	[0,0]	-9	hSpCas9	negative selection	4496463
25227299	25227321	12	-	27760321	OCIAML2	viability	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.5075548601066703	[569,439]	[475,708]	7	hSpCas9	negative selection	4496464
25227337	25227359	12	-	27760321	OCIAML2	viability	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.2464048307182444	[366,348]	[327,364]	3	hSpCas9	negative selection	4496465
25227354	25227376	12	+	27760321	OCIAML2	viability	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.06874643539315461	[130,124]	[97,99]	-1	hSpCas9	negative selection	4496466
25225682	25225704	12	+	27760321	MV411	viability	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	0.8184529446595543	[63,84]	[71,111]	6	hSpCas9	negative selection	4579925
25227230	25227252	12	-	27760321	MV411	viability	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-3.4079176457795963	[8,21]	[0,0]	-9	hSpCas9	negative selection	4579926
25227299	25227321	12	-	27760321	MV411	viability	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	-0.904584526736532	[569,439]	[81,318]	-6	hSpCas9	negative selection	4579927
25227337	25227359	12	-	27760321	MV411	viability	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.08933783882005181	[366,348]	[137,348]	0	hSpCas9	negative selection	4579928
25227354	25227376	12	+	27760321	MV411	viability	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.9824304124835312	[130,124]	[53,25]	-6	hSpCas9	negative selection	4579929
25225682	25225704	12	+	27760321	HL60	viability	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-0.7845869880120309	[63,84]	[30,40]	-7	hSpCas9	negative selection	4663388
25227230	25227252	12	-	27760321	HL60	viability	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-3.715787807573733	[8,21]	[0,0]	-9	hSpCas9	negative selection	4663389
25227299	25227321	12	-	27760321	HL60	viability	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	-0.269000402705996	[569,439]	[301,379]	-3	hSpCas9	negative selection	4663390
25227337	25227359	12	-	27760321	HL60	viability	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.1307808390686689	[366,348]	[256,387]	1	hSpCas9	negative selection	4663391
25227354	25227376	12	+	27760321	HL60	viability	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.07884601773071664	[130,124]	[86,111]	-1	hSpCas9	negative selection	4663392
25225682	25225704	12	+	27760321	HT1080	viability	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-1.4117101793406763	[84,66]	[8,13]	-5	hSpCas9	negative selection	4746851
25227230	25227252	12	-	27760321	HT1080	viability	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-2.95669589080409	[21,16]	[0,0]	-7	hSpCas9	negative selection	4746852
25227299	25227321	12	-	27760321	HT1080	viability	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	1.0648073021869529	[439,343]	[446,194]	6	hSpCas9	negative selection	4746853
25227337	25227359	12	-	27760321	HT1080	viability	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	1.5938458059224674	[348,272]	[479,257]	8	hSpCas9	negative selection	4746854
25227354	25227376	12	+	27760321	HT1080	viability	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	1.7242352471132087	[124,97]	[110,185]	8	hSpCas9	negative selection	4746855
25225682	25225704	12	+	27760321	HT29	viability after 7 days	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-0.962751727540053	[84,66]	[38,64,44]	-8	hSpCas9	negative selection	4830314
25227230	25227252	12	-	27760321	HT29	viability after 7 days	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-0.40882038195504755	[21,16]	[0,0,50]	-6	hSpCas9	negative selection	4830315
25227299	25227321	12	-	27760321	HT29	viability after 7 days	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.2878268414327094	[439,343]	[729,690,445]	4	hSpCas9	negative selection	4830316
25227337	25227359	12	-	27760321	HT29	viability after 7 days	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.2177303242905968	[348,272]	[574,427,408]	3	hSpCas9	negative selection	4830317
25227354	25227376	12	+	27760321	HT29	viability after 7 days	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	0.20566299609073968	[124,97]	[83,162,234]	3	hSpCas9	negative selection	4830318
25225682	25225704	12	+	27760321	HT29	viability after 25 days	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-0.3674943563271831	[84,66]	[112,36,46]	-4	hSpCas9	negative selection	4913777
25227230	25227252	12	-	27760321	HT29	viability after 25 days	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-4.476378155650614	[21,16]	[0,0,0]	-9	hSpCas9	negative selection	4913778
25227299	25227321	12	-	27760321	HT29	viability after 25 days	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.16044973120131564	[439,343]	[368,456,704]	1	hSpCas9	negative selection	4913779
25227337	25227359	12	-	27760321	HT29	viability after 25 days	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.26890941908414545	[348,272]	[483,417,372]	2	hSpCas9	negative selection	4913780
25227354	25227376	12	+	27760321	HT29	viability after 25 days	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.5659080574616739	[124,97]	[69,121,64]	-5	hSpCas9	negative selection	4913781
25225682	25225704	12	+	27760321	HT29	viability after 22 days	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-0.769074479046369	[84,66]	[99,48,3]	-6	hSpCas9	negative selection	4997240
25227230	25227252	12	-	27760321	HT29	viability after 22 days	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-1.998122372894966	[21,16]	[0,14,0]	-8	hSpCas9	negative selection	4997241
25227299	25227321	12	-	27760321	HT29	viability after 22 days	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.015250437326090854	[439,343]	[251,424,704]	0	hSpCas9	negative selection	4997242
25227337	25227359	12	-	27760321	HT29	viability after 22 days	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.1884869320985561	[348,272]	[356,433,439]	2	hSpCas9	negative selection	4997243
25227354	25227376	12	+	27760321	HT29	viability after 22 days	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.732466297703958	[124,97]	[65,101,64]	-6	hSpCas9	negative selection	4997244
25225682	25225704	12	+	27760321	HT29	viability after 19 days	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-1.2534066120561187	[84,66]	[47,62,0]	-7	hSpCas9	negative selection	5080703
25227230	25227252	12	-	27760321	HT29	viability after 19 days	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-4.47785673508666	[21,16]	[0,0,0]	-9	hSpCas9	negative selection	5080704
25227299	25227321	12	-	27760321	HT29	viability after 19 days	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	-0.17497297228630151	[439,343]	[386,428,374]	-2	hSpCas9	negative selection	5080705
25227337	25227359	12	-	27760321	HT29	viability after 19 days	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.7834326501266329	[348,272]	[281,829,686]	7	hSpCas9	negative selection	5080706
25227354	25227376	12	+	27760321	HT29	viability after 19 days	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-1.0836041272677286	[124,97]	[70,32,76]	-6	hSpCas9	negative selection	5080707
25225682	25225704	12	+	27760321	HT29	viability after 16 days	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-0.08088766351523491	[84,66]	[75,152,9]	-1	hSpCas9	negative selection	5164166
25227230	25227252	12	-	27760321	HT29	viability after 16 days	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-1.4109704279896604	[21,16]	[0,4,17]	-8	hSpCas9	negative selection	5164167
25227299	25227321	12	-	27760321	HT29	viability after 16 days	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.02219492290637365	[439,343]	[534,316,495]	0	hSpCas9	negative selection	5164168
25227337	25227359	12	-	27760321	HT29	viability after 16 days	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.11931342839909112	[348,272]	[517,460,165]	1	hSpCas9	negative selection	5164169
25227354	25227376	12	+	27760321	HT29	viability after 16 days	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.8516975373092754	[124,97]	[111,48,50]	-6	hSpCas9	negative selection	5164170
25225682	25225704	12	+	27760321	HT29	viability after 13 days	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	0.15314016701979183	[84,66]	[84,190,22]	1	hSpCas9	negative selection	5247629
25227230	25227252	12	-	27760321	HT29	viability after 13 days	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-4.498679682109876	[21,16]	[0,0,0]	-9	hSpCas9	negative selection	5247630
25227299	25227321	12	-	27760321	HT29	viability after 13 days	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.20251036956812749	[439,343]	[561,520,488]	2	hSpCas9	negative selection	5247631
25227337	25227359	12	-	27760321	HT29	viability after 13 days	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.5528116916306126	[348,272]	[774,549,277]	7	hSpCas9	negative selection	5247632
25227354	25227376	12	+	27760321	HT29	viability after 13 days	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.825113176295377	[124,97]	[52,124,42]	-7	hSpCas9	negative selection	5247633
25225682	25225704	12	+	27760321	HT29	viability after 10 days	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-0.6472683337828551	[84,66]	[63,101,17]	-6	hSpCas9	negative selection	5331092
25227230	25227252	12	-	27760321	HT29	viability after 10 days	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-4.60469098927022	[21,16]	[0,0,0]	-9	hSpCas9	negative selection	5331093
25227299	25227321	12	-	27760321	HT29	viability after 10 days	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.30962054476481526	[439,343]	[492,699,628]	4	hSpCas9	negative selection	5331094
25227337	25227359	12	-	27760321	HT29	viability after 10 days	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.15814911552184135	[348,272]	[220,568,511]	2	hSpCas9	negative selection	5331095
25227354	25227376	12	+	27760321	HT29	viability after 10 days	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-0.7044866756078658	[124,97]	[52,92,109]	-7	hSpCas9	negative selection	5331096
25225682	25225704	12	+	27760321	MOLM13	viability	TTGTGTCTACTGTTCTAGAAGG	KRAS	ENSG00000133703	-5.414704342108516	[63,84]	[0,0]	-9	hSpCas9	negative selection	5414555
25227230	25227252	12	-	27760321	MOLM13	viability	AGATATTCACCATTATAGGTGG	KRAS	ENSG00000133703	-3.181860832952001	[8,21]	[0,0]	-9	hSpCas9	negative selection	5414556
25227299	25227321	12	-	27760321	MOLM13	viability	GGACCAGTACATGAGGACTGGG	KRAS	ENSG00000133703	0.668452042043385	[569,439]	[374,512]	4	hSpCas9	negative selection	5414557
25227337	25227359	12	-	27760321	MOLM13	viability	CTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.4951551127111339	[366,348]	[15,251]	-3	hSpCas9	negative selection	5414558
25227354	25227376	12	+	27760321	MOLM13	viability	TCGAGAATATCCAAGAGACAGG	KRAS	ENSG00000133703	-1.6299198493692657	[130,124]	[4,38]	-7	hSpCas9	negative selection	5414559
25250396	25250419	12	-	27661255	K562	viability	GGGCCGGCTGCCCCTCGGAGCGG	KRAS	ENSG00000133703	-0.13731174626504167	[705,910]	[678,503]	-5	dCas9-KRAB	negative selection	5489182
25227337	25227360	12	-	27260156	BXPC3	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.38891506513941054	[481]	[709,681,594,636]	8	hSpCas9	negative selection	5551519
25227305	25227328	12	-	27260156	BXPC3	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.14135382749435932	[239]	[243,321,235,306]	3	hSpCas9	negative selection	5551520
25209876	25209899	12	+	27260156	BXPC3	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.2815066968473959	[444]	[634,502,565,547]	6	hSpCas9	negative selection	5551521
25225720	25225743	12	-	27260156	BXPC3	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.13701243661194984	[497]	[447,532,485,415]	-3	hSpCas9	negative selection	5615775
25215475	25215498	12	-	27260156	BXPC3	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.3413104261373944	[290]	[263,210,185,303]	-6	hSpCas9	negative selection	5615776
25227337	25227360	12	-	27260156	A673	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.16911630259731916	[481]	[660,597,690,653]	4	hSpCas9	negative selection	5672770
25227305	25227328	12	-	27260156	A673	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.08986064736424704	[239]	[348,333,210,338]	2	hSpCas9	negative selection	5672771
25209876	25209899	12	+	27260156	A673	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.20824260904064973	[444]	[769,553,529,634]	5	hSpCas9	negative selection	5672772
25225720	25225743	12	-	27260156	A673	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.24870134155197476	[497]	[499,549,470,488]	-6	hSpCas9	negative selection	5737026
25215475	25215498	12	-	27260156	A673	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.6892449543351872	[290]	[251,272,109,235]	-9	hSpCas9	negative selection	5737027
25227337	25227360	12	-	27260156	A375	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.20186671432440226	[481]	[703,723,572,602]	3	hSpCas9	negative selection	5794021
25227305	25227328	12	-	27260156	A375	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.22241713898390048	[239]	[115,326,386,151]	-3	hSpCas9	negative selection	5794022
25209876	25209899	12	+	27260156	A375	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.4308923164548426	[444]	[713,700,729,659]	6	hSpCas9	negative selection	5794023
25225720	25225743	12	-	27260156	A375	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.24884001569709283	[497]	[490,569,496,417]	-4	hSpCas9	negative selection	5858277
25215475	25215498	12	-	27260156	A375	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.2587260630218685	[290]	[301,212,321,287]	-4	hSpCas9	negative selection	5858278
25227337	25227360	12	-	27260156	COLO741	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.6618669341421914	[481]	[703,1351,851]	9	hSpCas9	negative selection	5915272
25227305	25227328	12	-	27260156	COLO741	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.20682119842154134	[239]	[360,352,347]	4	hSpCas9	negative selection	5915273
25209876	25209899	12	+	27260156	COLO741	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.26155002014999307	[444]	[728,675,644]	5	hSpCas9	negative selection	5915274
25225720	25225743	12	-	27260156	COLO741	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.24438808029347225	[497]	[586,540,489]	-5	hSpCas9	negative selection	5979528
25215475	25215498	12	-	27260156	COLO741	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.3804616536580262	[290]	[244,337,270]	-7	hSpCas9	negative selection	5979529
25227337	25227360	12	-	27260156	CAL120	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.22146703607776605	[481]	[1091,771,299]	3	hSpCas9	negative selection	6036523
25227305	25227328	12	-	27260156	CAL120	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.1280446113128837	[239]	[372,313,149]	-2	hSpCas9	negative selection	6036524
25209876	25209899	12	+	27260156	CAL120	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.6524055243267508	[444]	[790,722,1080]	8	hSpCas9	negative selection	6036525
25225720	25225743	12	-	27260156	CAL120	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.44025177394841253	[497]	[480,455,436]	-6	hSpCas9	negative selection	6100779
25215475	25215498	12	-	27260156	CAL120	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.21195425428819226	[290]	[475,264,222]	-3	hSpCas9	negative selection	6100780
25227337	25227360	12	-	27260156	CADOES1	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.10531885085333181	[481]	[281,468,431,486]	-2	hSpCas9	negative selection	6157774
25227305	25227328	12	-	27260156	CADOES1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.3240527972253898	[239]	[230,326,176,386]	5	hSpCas9	negative selection	6157775
25209876	25209899	12	+	27260156	CADOES1	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.33937967638141986	[444]	[469,527,437,663]	6	hSpCas9	negative selection	6157776
25225720	25225743	12	-	27260156	CADOES1	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.23399126693045766	[497]	[421,282,398,473]	-4	hSpCas9	negative selection	6222030
25215475	25215498	12	-	27260156	CADOES1	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.23070706191500612	[290]	[82,313,196,332]	-4	hSpCas9	negative selection	6222031
25227337	25227360	12	-	27260156	EWS502	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.2602467726297079	[481]	[401,334,447,532]	-4	hSpCas9	negative selection	6279025
25227305	25227328	12	-	27260156	EWS502	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.3830973199352423	[239]	[90,234,236,229]	-6	hSpCas9	negative selection	6279026
25209876	25209899	12	+	27260156	EWS502	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.32130312457956434	[444]	[597,592,665,488]	5	hSpCas9	negative selection	6279027
25225720	25225743	12	-	27260156	EWS502	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.876828343048217	[497]	[251,350,260,284]	-8	hSpCas9	negative selection	6343281
25215475	25215498	12	-	27260156	EWS502	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-1.1833186960572943	[290]	[139,153,106,139]	-9	hSpCas9	negative selection	6343282
25227337	25227360	12	-	27260156	EW8	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.04913604793962348	[481]	[402,460,386,259]	-1	hSpCas9	negative selection	6400276
25227305	25227328	12	-	27260156	EW8	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.22102740256530407	[239]	[225,146,133,171]	-4	hSpCas9	negative selection	6400277
25209876	25209899	12	+	27260156	EW8	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.14042354614608987	[444]	[584,276,379,369]	2	hSpCas9	negative selection	6400278
25225720	25225743	12	-	27260156	EW8	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.32238844645056364	[497]	[357,357,264,322]	-6	hSpCas9	negative selection	6464532
25215475	25215498	12	-	27260156	EW8	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.7034363692068145	[290]	[155,151,97,181]	-8	hSpCas9	negative selection	6464533
25227337	25227360	12	-	27260156	CORL105	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.14095301253170406	[481]	[633,694,641,519]	2	hSpCas9	negative selection	6521527
25227305	25227328	12	-	27260156	CORL105	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.09374982822215461	[239]	[344,214,366,285]	1	hSpCas9	negative selection	6521528
25209876	25209899	12	+	27260156	CORL105	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.15777757262626557	[444]	[436,638,599,644]	3	hSpCas9	negative selection	6521529
25225720	25225743	12	-	27260156	CORL105	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.2209338428170756	[497]	[415,449,529,606]	-4	hSpCas9	negative selection	6585783
25215475	25215498	12	-	27260156	CORL105	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	0.013141116817438125	[290]	[374,354,330,312]	0	hSpCas9	negative selection	6585784
25227337	25227360	12	-	27260156	HS294T	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.9640957648062642	[481]	[77,73,102,135]	-8	hSpCas9	negative selection	6642778
25227305	25227328	12	-	27260156	HS294T	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.07040965441311009	[239]	[16,18,105,246]	-1	hSpCas9	negative selection	6642779
25209876	25209899	12	+	27260156	HS294T	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-0.159266464033942	[444]	[104,121,138,275]	-2	hSpCas9	negative selection	6642780
25225720	25225743	12	-	27260156	HS294T	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.944755336921681	[497]	[57,38,118,207]	-8	hSpCas9	negative selection	6707034
25215475	25215498	12	-	27260156	HS294T	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.8280871535000497	[290]	[57,65,49,82]	-8	hSpCas9	negative selection	6707035
25227337	25227360	12	-	27260156	HCC44	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-2.823465337842956	[481]	[53,84,82,82]	-9	hSpCas9	negative selection	6764029
25227305	25227328	12	-	27260156	HCC44	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.7391009538465287	[239]	[73,92,98,59]	-9	hSpCas9	negative selection	6764030
25209876	25209899	12	+	27260156	HCC44	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-0.0699636228285382	[444]	[487,313,571,576]	-1	hSpCas9	negative selection	6764031
25225720	25225743	12	-	27260156	HCC44	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-1.7912893530842302	[497]	[163,222,220,46]	-9	hSpCas9	negative selection	6828285
25215475	25215498	12	-	27260156	HCC44	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.3290222030623108	[290]	[340,155,368,221]	-5	hSpCas9	negative selection	6828286
25227337	25227360	12	-	27260156	G402	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.07593341213512884	[481]	[545,579,775,564]	0	hSpCas9	negative selection	6885280
25227305	25227328	12	-	27260156	G402	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.35844681111589405	[239]	[69,91,639,86]	-4	hSpCas9	negative selection	6885281
25209876	25209899	12	+	27260156	G402	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.241679733066561	[444]	[471,654,798,616]	3	hSpCas9	negative selection	6885282
25225720	25225743	12	-	27260156	G402	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.05359124324160551	[497]	[472,450,897,497]	0	hSpCas9	negative selection	6949536
25215475	25215498	12	-	27260156	G402	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.3537566316413826	[290]	[445,199,372,118]	-4	hSpCas9	negative selection	6949537
25227337	25227360	12	-	27260156	L33	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.021481019039358687	[481]	[530,198,332,352]	0	hSpCas9	negative selection	7006531
25227305	25227328	12	-	27260156	L33	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.1903089580680447	[239]	[330,83,166,291]	4	hSpCas9	negative selection	7006532
25209876	25209899	12	+	27260156	L33	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.14662040757067446	[444]	[435,158,418,481]	3	hSpCas9	negative selection	7006533
25225720	25225743	12	-	27260156	L33	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.2922925098113677	[497]	[404,131,261,481]	-6	hSpCas9	negative selection	7070787
25215475	25215498	12	-	27260156	L33	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.3754281008270334	[290]	[174,98,107,308]	-7	hSpCas9	negative selection	7070788
25227337	25227360	12	-	27260156	K562	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.39414646818690635	[481]	[168,255,711,165]	-5	hSpCas9	negative selection	7127782
25227305	25227328	12	-	27260156	K562	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.01321401912340614	[239]	[137,264,310,148]	0	hSpCas9	negative selection	7127783
25209876	25209899	12	+	27260156	K562	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.5597158375382262	[444]	[803,619,405,522]	7	hSpCas9	negative selection	7127784
25225720	25225743	12	-	27260156	K562	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.4367528339368544	[497]	[432,311,263,305]	-5	hSpCas9	negative selection	7192038
25215475	25215498	12	-	27260156	K562	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	0.012499368917100795	[290]	[265,345,363,89]	0	hSpCas9	negative selection	7192039
25227337	25227360	12	-	27260156	HT29	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.19495574471867966	[481]	[738,726,685,550]	4	hSpCas9	negative selection	7249033
25227305	25227328	12	-	27260156	HT29	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.004503368894010817	[239]	[412,276,243,247]	0	hSpCas9	negative selection	7249034
25209876	25209899	12	+	27260156	HT29	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.36195047170313804	[444]	[658,950,669,534]	7	hSpCas9	negative selection	7249035
25225720	25225743	12	-	27260156	HT29	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.2424491647568659	[497]	[614,582,497,377]	-5	hSpCas9	negative selection	7313289
25215475	25215498	12	-	27260156	HT29	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.7104049531117876	[290]	[269,339,159,119]	-8	hSpCas9	negative selection	7313290
25227337	25227360	12	-	27260156	MHHES1	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.019974713916380143	[481]	[684,422,595,451]	0	hSpCas9	negative selection	7370284
25227305	25227328	12	-	27260156	MHHES1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.14788053374178056	[239]	[317,310,121,235]	-3	hSpCas9	negative selection	7370285
25209876	25209899	12	+	27260156	MHHES1	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.5476331599262564	[444]	[748,693,966,546]	8	hSpCas9	negative selection	7370286
25225720	25225743	12	-	27260156	MHHES1	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.6303423714801806	[497]	[240,520,438,264]	-8	hSpCas9	negative selection	7434540
25215475	25215498	12	-	27260156	MHHES1	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.4568067405085089	[290]	[191,345,296,142]	-7	hSpCas9	negative selection	7434541
25227337	25227360	12	-	27260156	MEWO	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.5076409228630826	[481]	[343,247,364]	-8	hSpCas9	negative selection	7491535
25227305	25227328	12	-	27260156	MEWO	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.23020254855865796	[239]	[345,280,178]	5	hSpCas9	negative selection	7491536
25209876	25209899	12	+	27260156	MEWO	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-0.07114277884818011	[444]	[299,423,445]	-1	hSpCas9	negative selection	7491537
25225720	25225743	12	-	27260156	MEWO	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	0.004380409638671243	[497]	[462,474,462]	0	hSpCas9	negative selection	7555791
25215475	25215498	12	-	27260156	MEWO	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.7640536464762749	[290]	[232,155,105]	-9	hSpCas9	negative selection	7555792
25227337	25227360	12	-	27260156	LNCAPCLONEFGC	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.16888853293945402	[481]	[589,415,550,417]	2	hSpCas9	negative selection	7612786
25227305	25227328	12	-	27260156	LNCAPCLONEFGC	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.10524862689301295	[239]	[164,129,452,227]	1	hSpCas9	negative selection	7612787
25209876	25209899	12	+	27260156	LNCAPCLONEFGC	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.40400390758195903	[444]	[461,432,794,489]	6	hSpCas9	negative selection	7612788
25225720	25225743	12	-	27260156	LNCAPCLONEFGC	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.3249807932182492	[497]	[369,251,553,299]	-5	hSpCas9	negative selection	7677042
25215475	25215498	12	-	27260156	LNCAPCLONEFGC	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.15096053492748807	[290]	[218,213,355,179]	-2	hSpCas9	negative selection	7677043
25227337	25227360	12	-	27260156	PANC0327	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-2.4444578152435037	[481]	[104,38,127,49]	-9	hSpCas9	negative selection	7734037
25227305	25227328	12	-	27260156	PANC0327	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-2.179468398713623	[239]	[78,14,48,51]	-9	hSpCas9	negative selection	7734038
25209876	25209899	12	+	27260156	PANC0327	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.44575353846926197	[444]	[291,413,932,510]	6	hSpCas9	negative selection	7734039
25225720	25225743	12	-	27260156	PANC0327	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-1.4532256686074743	[497]	[154,174,196,116]	-9	hSpCas9	negative selection	7798293
25215475	25215498	12	-	27260156	PANC0327	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.34986201913952	[290]	[258,86,297,186]	-5	hSpCas9	negative selection	7798294
25227337	25227360	12	-	27260156	NCIH2009	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-2.055087304664114	[481]	[229,233,33]	-9	hSpCas9	negative selection	7855288
25227305	25227328	12	-	27260156	NCIH2009	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.116092795346386	[239]	[173,189,102]	-8	hSpCas9	negative selection	7855289
25209876	25209899	12	+	27260156	NCIH2009	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-0.4038829076817513	[444]	[576,476,375]	-5	hSpCas9	negative selection	7855290
25225720	25225743	12	-	27260156	NCIH2009	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-2.307222527937521	[497]	[203,142,86]	-9	hSpCas9	negative selection	7919544
25215475	25215498	12	-	27260156	NCIH2009	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.37796124635712414	[290]	[507,237,231]	-5	hSpCas9	negative selection	7919545
25227337	25227360	12	-	27260156	NCIH1373	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-3.100549801024528	[481]	[49,106,37,12]	-9	hSpCas9	negative selection	7976539
25227305	25227328	12	-	27260156	NCIH1373	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.1382920752483263	[239]	[99,162,102,29]	-8	hSpCas9	negative selection	7976540
25209876	25209899	12	+	27260156	NCIH1373	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.39720369985775766	[444]	[561,723,485,221]	4	hSpCas9	negative selection	7976541
25225720	25225743	12	-	27260156	NCIH1373	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-2.2992965486003776	[497]	[208,72,76,29]	-9	hSpCas9	negative selection	8040795
25215475	25215498	12	-	27260156	NCIH1373	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.05363722435580953	[290]	[562,254,148,88]	0	hSpCas9	negative selection	8040796
25227337	25227360	12	-	27260156	PATU8902	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-4.861725302924016	[481]	[64,3,2,24]	-9	hSpCas9	negative selection	8097790
25227305	25227328	12	-	27260156	PATU8902	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-2.023595131353358	[239]	[23,160,8,163]	-9	hSpCas9	negative selection	8097791
25209876	25209899	12	+	27260156	PATU8902	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-1.8626386855354973	[444]	[111,120,247,180]	-9	hSpCas9	negative selection	8097792
25225720	25225743	12	-	27260156	PATU8902	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-2.2558837171634196	[497]	[425,30,52,81]	-9	hSpCas9	negative selection	8162046
25215475	25215498	12	-	27260156	PATU8902	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.21565261845046202	[290]	[176,582,67,680]	-2	hSpCas9	negative selection	8162047
25227337	25227360	12	-	27260156	PANC1	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-4.279655434472543	[481]	[14,14,26,25]	-9	hSpCas9	negative selection	8219041
25227305	25227328	12	-	27260156	PANC1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.9550467967674419	[239]	[42,47,59,58]	-9	hSpCas9	negative selection	8219042
25209876	25209899	12	+	27260156	PANC1	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-0.26477511182140107	[444]	[303,554,291,186]	-4	hSpCas9	negative selection	8219043
25225720	25225743	12	-	27260156	PANC1	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-1.5756484622107991	[497]	[186,282,63,75]	-9	hSpCas9	negative selection	8283297
25215475	25215498	12	-	27260156	PANC1	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.763722263095019	[290]	[162,169,219,53]	-8	hSpCas9	negative selection	8283298
25227337	25227360	12	-	27260156	PANC0813	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-1.7964915226624438	[481]	[127,86,184,243]	-9	hSpCas9	negative selection	8340292
25227305	25227328	12	-	27260156	PANC0813	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.8272162084551586	[239]	[66,49,107,90]	-9	hSpCas9	negative selection	8340293
25209876	25209899	12	+	27260156	PANC0813	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.07071832918377191	[444]	[552,437,673,531]	1	hSpCas9	negative selection	8340294
25225720	25225743	12	-	27260156	PANC0813	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-1.7305505049702146	[497]	[134,139,260,165]	-9	hSpCas9	negative selection	8404548
25215475	25215498	12	-	27260156	PANC0813	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.12182013116310242	[290]	[374,366,305,218]	-2	hSpCas9	negative selection	8404549
25227337	25227360	12	-	27260156	RDES	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.2744759492886526	[481]	[412,412,500,515]	-5	hSpCas9	negative selection	8461543
25227305	25227328	12	-	27260156	RDES	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.331040415720842	[239]	[166,228,196,299]	-5	hSpCas9	negative selection	8461544
25209876	25209899	12	+	27260156	RDES	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.20796920973446675	[444]	[492,504,682,717]	3	hSpCas9	negative selection	8461545
25225720	25225743	12	-	27260156	RDES	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.5630164838940988	[497]	[325,330,316,615]	-7	hSpCas9	negative selection	8525799
25215475	25215498	12	-	27260156	RDES	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.4676539927756326	[290]	[222,195,188,379]	-7	hSpCas9	negative selection	8525800
25227337	25227360	12	-	27260156	PC3	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.07247241056076678	[481]	[345,330,880,642]	1	hSpCas9	negative selection	8582794
25227305	25227328	12	-	27260156	PC3	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.023611316653819436	[239]	[179,234,511,146]	0	hSpCas9	negative selection	8582795
25209876	25209899	12	+	27260156	PC3	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.22388735695735268	[444]	[327,446,905,581]	5	hSpCas9	negative selection	8582796
25225720	25225743	12	-	27260156	PC3	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.09557898587658448	[497]	[262,494,817,464]	-2	hSpCas9	negative selection	8647050
25215475	25215498	12	-	27260156	PC3	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	0.3749280455349318	[290]	[67,149,1614,288]	7	hSpCas9	negative selection	8647051
25227337	25227360	12	-	27260156	PATU8988T	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-1.9466597550883846	[481]	[63,231,89,106]	-9	hSpCas9	negative selection	8704045
25227305	25227328	12	-	27260156	PATU8988T	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.7118684746166533	[239]	[113,33,88,39]	-9	hSpCas9	negative selection	8704046
25209876	25209899	12	+	27260156	PATU8988T	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-0.2530461182431332	[444]	[457,456,293,233]	-4	hSpCas9	negative selection	8704047
25225720	25225743	12	-	27260156	PATU8988T	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-1.0444316953280426	[497]	[247,181,234,265]	-9	hSpCas9	negative selection	8768301
25215475	25215498	12	-	27260156	PATU8988T	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.12405311034945798	[290]	[222,148,350,300]	-2	hSpCas9	negative selection	8768302
25227337	25227360	12	-	27260156	T47D	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.09725036342402335	[481]	[591,542,470,491]	-2	hSpCas9	negative selection	8825296
25227305	25227328	12	-	27260156	T47D	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.08523892680276857	[239]	[326,352,266,232]	1	hSpCas9	negative selection	8825297
25209876	25209899	12	+	27260156	T47D	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.24296901900518886	[444]	[626,665,612,540]	5	hSpCas9	negative selection	8825298
25225720	25225743	12	-	27260156	T47D	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.4140253505539244	[497]	[406,364,496,478]	-7	hSpCas9	negative selection	8889552
25215475	25215498	12	-	27260156	T47D	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.2289271698407479	[290]	[209,286,319,340]	-5	hSpCas9	negative selection	8889553
25227337	25227360	12	-	27260156	SU8686	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-2.5543656584798837	[481]	[42,116,61,105]	-9	hSpCas9	negative selection	8946547
25227305	25227328	12	-	27260156	SU8686	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.8003686173722746	[239]	[48,60,71,98]	-9	hSpCas9	negative selection	8946548
25209876	25209899	12	+	27260156	SU8686	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.06186029341855447	[444]	[455,396,420,593]	1	hSpCas9	negative selection	8946549
25225720	25225743	12	-	27260156	SU8686	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-2.2959479831363536	[497]	[95,74,135,98]	-9	hSpCas9	negative selection	9010803
25215475	25215498	12	-	27260156	SU8686	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.4443121109631135	[290]	[273,188,191,181]	-6	hSpCas9	negative selection	9010804
25227337	25227360	12	-	27260156	SKES1	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.014852762092818432	[481]	[516,360,559,487]	0	hSpCas9	negative selection	9067798
25227305	25227328	12	-	27260156	SKES1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.3394018068200553	[239]	[161,230,201,163]	-6	hSpCas9	negative selection	9067799
25209876	25209899	12	+	27260156	SKES1	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.29747508569360465	[444]	[587,546,574,479]	6	hSpCas9	negative selection	9067800
25225720	25225743	12	-	27260156	SKES1	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.009187054382694418	[497]	[561,518,450,454]	0	hSpCas9	negative selection	9132054
25215475	25215498	12	-	27260156	SKES1	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.8054933935036294	[290]	[172,183,173,137]	-9	hSpCas9	negative selection	9132055
25227337	25227360	12	-	27260156	TOV112D	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	0.1176064891720762	[481]	[551,819,439,361]	2	hSpCas9	negative selection	9189049
25227305	25227328	12	-	27260156	TOV112D	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.018793653204842276	[239]	[270,237,180,260]	0	hSpCas9	negative selection	9189050
25209876	25209899	12	+	27260156	TOV112D	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.231100823744809	[444]	[551,793,410,406]	4	hSpCas9	negative selection	9189051
25225720	25225743	12	-	27260156	TOV112D	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.20490612452453716	[497]	[506,461,353,420]	-4	hSpCas9	negative selection	9253305
25215475	25215498	12	-	27260156	TOV112D	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	0.08019620057369237	[290]	[178,684,365,89]	1	hSpCas9	negative selection	9253306
25227337	25227360	12	-	27260156	TC71	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.01178243055142348	[481]	[489,457,466,644]	0	hSpCas9	negative selection	9310300
25227305	25227328	12	-	27260156	TC71	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.2379927759461773	[239]	[196,234,290,142]	-6	hSpCas9	negative selection	9310301
25209876	25209899	12	+	27260156	TC71	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	-0.01592156772537237	[444]	[549,439,363,538]	0	hSpCas9	negative selection	9310302
25225720	25225743	12	-	27260156	TC71	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.20355942545618771	[497]	[358,489,531,473]	-5	hSpCas9	negative selection	9374556
25215475	25215498	12	-	27260156	TC71	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.3668241460891891	[290]	[194,234,273,264]	-7	hSpCas9	negative selection	9374557
25227337	25227360	12	-	27260156	TC32	viability	TCTCGACACAGCAGGTCAAGAGG	KRAS	ENSG00000133703	-0.14981454258605764	[481]	[420,285,519,447]	-3	hSpCas9	negative selection	9431551
25227305	25227328	12	-	27260156	TC32	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.453426343560295	[239]	[160,130,273,111]	-7	hSpCas9	negative selection	9431552
25209876	25209899	12	+	27260156	TC32	viability	TTCTCGAACTAATGTATAGAAGG	KRAS	ENSG00000133703	0.11766500279350295	[444]	[414,438,486,460]	2	hSpCas9	negative selection	9431553
25225720	25225743	12	-	27260156	TC32	viability	GATGTACCTATGGTCCTAGTAGG	KRAS	ENSG00000133703	-0.5092411309819211	[497]	[262,203,580,349]	-7	hSpCas9	negative selection	9495807
25215475	25215498	12	-	27260156	TC32	viability	AGCAAAGAAGAAAAGACTCCTGG	KRAS	ENSG00000133703	-0.5481469506621287	[290]	[191,167,234,153]	-8	hSpCas9	negative selection	9495808
25227295	25227318	12	-	28162770	P31FUJ	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	0.3741028491874548	[173]	[264]	4	hSpCas9	negative selection	10181146
25245310	25245333	12	+	28162770	P31FUJ	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.01833011236312343	[141]	[168]	0	hSpCas9	negative selection	10181147
25245341	25245364	12	-	28162770	P31FUJ	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	0.8005349794597341	[384]	[787]	8	hSpCas9	negative selection	10181148
25227320	25227343	12	-	28162770	P31FUJ	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.4646350255651902	[221]	[359]	5	hSpCas9	negative selection	10181149
25225730	25225753	12	-	28162770	P31FUJ	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.8253875592576281	[327]	[682]	8	hSpCas9	negative selection	10181150
25225670	25225693	12	-	28162770	P31FUJ	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.9700940412962722	[148]	[342]	9	hSpCas9	negative selection	10181151
25227305	25227328	12	-	28162770	P31FUJ	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.4808936104289805	[174]	[286]	5	hSpCas9	negative selection	10181152
25227345	25227368	12	-	28162770	P31FUJ	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.4783374023839224	[495]	[811]	5	hSpCas9	negative selection	10181153
25227295	25227318	12	-	28162770	TF1	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	NaN	[NaN]	[78]	1	hSpCas9	negative selection	10350512
25245310	25245333	12	+	28162770	TF1	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	NaN	[NaN]	[270]	1	hSpCas9	negative selection	10350513
25245341	25245364	12	-	28162770	TF1	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	NaN	[NaN]	[9]	1	hSpCas9	negative selection	10350514
25227320	25227343	12	-	28162770	TF1	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	NaN	[NaN]	[36]	1	hSpCas9	negative selection	10350515
25225730	25225753	12	-	28162770	TF1	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	NaN	[NaN]	[48]	1	hSpCas9	negative selection	10350516
25225670	25225693	12	-	28162770	TF1	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	NaN	[NaN]	[38]	1	hSpCas9	negative selection	10350517
25227305	25227328	12	-	28162770	TF1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	NaN	[NaN]	[41]	1	hSpCas9	negative selection	10350518
25227345	25227368	12	-	28162770	TF1	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	NaN	[NaN]	[592]	1	hSpCas9	negative selection	10350519
25227295	25227318	12	-	28162770	SKM1	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-2.217930374353145	[49]	[18]	-8	hSpCas9	negative selection	10519878
25245310	25245333	12	+	28162770	SKM1	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	-0.32450203855118853	[33]	[47]	-3	hSpCas9	negative selection	10519879
25245341	25245364	12	-	28162770	SKM1	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-4.384937892413163	[129]	[10]	-9	hSpCas9	negative selection	10519880
25227320	25227343	12	-	28162770	SKM1	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-4.540819945477953	[78]	[5]	-9	hSpCas9	negative selection	10519881
25225730	25225753	12	-	28162770	SKM1	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-4.016379743188857	[118]	[12]	-9	hSpCas9	negative selection	10519882
25225670	25225693	12	-	28162770	SKM1	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	-3.034995421356204	[50]	[10]	-9	hSpCas9	negative selection	10519883
25227305	25227328	12	-	28162770	SKM1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-4.366322214245816	[34]	[2]	-9	hSpCas9	negative selection	10519884
25227345	25227368	12	-	28162770	SKM1	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	-1.2294260131909742	[125]	[94]	-7	hSpCas9	negative selection	10519885
25227295	25227318	12	-	28162770	SKM1	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-2.217930374353145	[49]	[18]	-8	hSpCas9	negative selection	10689244
25245310	25245333	12	+	28162770	SKM1	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	-0.32450203855118853	[33]	[47]	-3	hSpCas9	negative selection	10689245
25245341	25245364	12	-	28162770	SKM1	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-4.384937892413163	[129]	[10]	-9	hSpCas9	negative selection	10689246
25227320	25227343	12	-	28162770	SKM1	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-4.540819945477953	[78]	[5]	-9	hSpCas9	negative selection	10689247
25225730	25225753	12	-	28162770	SKM1	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-4.016379743188857	[118]	[12]	-9	hSpCas9	negative selection	10689248
25225670	25225693	12	-	28162770	SKM1	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	-3.034995421356204	[50]	[10]	-9	hSpCas9	negative selection	10689249
25227305	25227328	12	-	28162770	SKM1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-4.366322214245816	[34]	[2]	-9	hSpCas9	negative selection	10689250
25227345	25227368	12	-	28162770	SKM1	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	-1.2294260131909742	[125]	[94]	-7	hSpCas9	negative selection	10689251
25227295	25227318	12	-	28162770	PL21	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.7366665590234858	[133]	[34]	-5	hSpCas9	negative selection	10858610
25245310	25245333	12	+	28162770	PL21	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.007494536546924557	[79]	[34]	0	hSpCas9	negative selection	10858611
25245341	25245364	12	-	28162770	PL21	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-7.623227625556916	[452]	[0]	-9	hSpCas9	negative selection	10858612
25227320	25227343	12	-	28162770	PL21	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-3.057876716577849	[286]	[14]	-9	hSpCas9	negative selection	10858613
25225730	25225753	12	-	28162770	PL21	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-2.5891813847380223	[317]	[22]	-9	hSpCas9	negative selection	10858614
25225670	25225693	12	-	28162770	PL21	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	-1.7028986556235919	[186]	[24]	-8	hSpCas9	negative selection	10858615
25227305	25227328	12	-	28162770	PL21	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.11267677323898062	[67]	[31]	1	hSpCas9	negative selection	10858616
25227345	25227368	12	-	28162770	PL21	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.6609227039897463	[387]	[266]	6	hSpCas9	negative selection	10858617
25227295	25227318	12	-	28162770	P31FUJ	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	0.3741028491874548	[173]	[264]	4	hSpCas9	negative selection	11027976
25245310	25245333	12	+	28162770	P31FUJ	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.01833011236312343	[141]	[168]	0	hSpCas9	negative selection	11027977
25245341	25245364	12	-	28162770	P31FUJ	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	0.8005349794597341	[384]	[787]	8	hSpCas9	negative selection	11027978
25227320	25227343	12	-	28162770	P31FUJ	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.4646350255651902	[221]	[359]	5	hSpCas9	negative selection	11027979
25225730	25225753	12	-	28162770	P31FUJ	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.8253875592576281	[327]	[682]	8	hSpCas9	negative selection	11027980
25225670	25225693	12	-	28162770	P31FUJ	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.9700940412962722	[148]	[342]	9	hSpCas9	negative selection	11027981
25227305	25227328	12	-	28162770	P31FUJ	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.4808936104289805	[174]	[286]	5	hSpCas9	negative selection	11027982
25227345	25227368	12	-	28162770	P31FUJ	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.4783374023839224	[495]	[811]	5	hSpCas9	negative selection	11027983
25227295	25227318	12	-	28162770	OCIAML5	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-1.3301138676686413	[77]	[55]	-6	hSpCas9	negative selection	11197342
25245310	25245333	12	+	28162770	OCIAML5	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	-0.2624426927729779	[102]	[154]	-2	hSpCas9	negative selection	11197343
25245341	25245364	12	-	28162770	OCIAML5	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-0.7718962221800137	[174]	[184]	-5	hSpCas9	negative selection	11197344
25227320	25227343	12	-	28162770	OCIAML5	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.582217017359491	[120]	[326]	5	hSpCas9	negative selection	11197345
25225730	25225753	12	-	28162770	OCIAML5	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-0.28140245319540935	[168]	[250]	-2	hSpCas9	negative selection	11197346
25225670	25225693	12	-	28162770	OCIAML5	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.19586790619970507	[73]	[152]	1	hSpCas9	negative selection	11197347
25227305	25227328	12	-	28162770	OCIAML5	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	1.020058606583845	[55]	[204]	8	hSpCas9	negative selection	11197348
25227345	25227368	12	-	28162770	OCIAML5	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	1.014994597317875	[212]	[776]	8	hSpCas9	negative selection	11197349
25227295	25227318	12	-	28162770	EOL1	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.7030066058750896	[274]	[88]	-5	hSpCas9	negative selection	11366708
25245310	25245333	12	+	28162770	EOL1	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	-0.026542627948518804	[115]	[59]	0	hSpCas9	negative selection	11366709
25245341	25245364	12	-	28162770	EOL1	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-1.9885063154303437	[820]	[108]	-8	hSpCas9	negative selection	11366710
25227320	25227343	12	-	28162770	EOL1	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-0.8321810774171017	[391]	[115]	-5	hSpCas9	negative selection	11366711
25225730	25225753	12	-	28162770	EOL1	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-0.974168892592284	[659]	[176]	-6	hSpCas9	negative selection	11366712
25225670	25225693	12	-	28162770	EOL1	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.3995673756776108	[317]	[220]	3	hSpCas9	negative selection	11366713
25227305	25227328	12	-	28162770	EOL1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.223350923541779	[194]	[87]	-2	hSpCas9	negative selection	11366714
25227345	25227368	12	-	28162770	EOL1	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	1.3381153827539238	[490]	[653]	9	hSpCas9	negative selection	11366715
25227295	25227318	12	-	28162770	MOLM13	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	0.873528182821173	[230]	[305]	6	hSpCas9	negative selection	11536074
25245310	25245333	12	+	28162770	MOLM13	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	1.4288952499287153	[149]	[291]	9	hSpCas9	negative selection	11536075
25245341	25245364	12	-	28162770	MOLM13	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-0.5670704568092212	[542]	[264]	-3	hSpCas9	negative selection	11536076
25227320	25227343	12	-	28162770	MOLM13	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.48820936592417463	[422]	[428]	3	hSpCas9	negative selection	11536077
25225730	25225753	12	-	28162770	MOLM13	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.3361307933737011	[400]	[365]	2	hSpCas9	negative selection	11536078
25225670	25225693	12	-	28162770	MOLM13	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.6370471960286956	[208]	[234]	5	hSpCas9	negative selection	11536079
25227305	25227328	12	-	28162770	MOLM13	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.3657089867381005	[116]	[108]	2	hSpCas9	negative selection	11536080
25227345	25227368	12	-	28162770	MOLM13	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.6260957844485335	[672]	[750]	5	hSpCas9	negative selection	11536081
25227295	25227318	12	-	28162770	HEL	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.06352996494293278	[185]	[113]	0	hSpCas9	negative selection	11705440
25245310	25245333	12	+	28162770	HEL	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	1.2066397171936833	[160]	[237]	9	hSpCas9	negative selection	11705441
25245341	25245364	12	-	28162770	HEL	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	0.07832370868328642	[487]	[329]	0	hSpCas9	negative selection	11705442
25227320	25227343	12	-	28162770	HEL	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.9909782040035664	[325]	[414]	8	hSpCas9	negative selection	11705443
25225730	25225753	12	-	28162770	HEL	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.5662406945263134	[425]	[403]	5	hSpCas9	negative selection	11705444
25225670	25225693	12	-	28162770	HEL	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.12903501444902277	[236]	[165]	1	hSpCas9	negative selection	11705445
25227305	25227328	12	-	28162770	HEL	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.579653589336091	[139]	[29]	-7	hSpCas9	negative selection	11705446
25227345	25227368	12	-	28162770	HEL	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.6038659403560669	[488]	[475]	5	hSpCas9	negative selection	11705447
25227295	25227318	12	-	28162770	MV411	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.713391851936547	[108]	[55]	-5	hSpCas9	negative selection	11874806
25245310	25245333	12	+	28162770	MV411	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.6485358591037454	[155]	[205]	6	hSpCas9	negative selection	11874807
25245341	25245364	12	-	28162770	MV411	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-0.900461144329538	[389]	[175]	-6	hSpCas9	negative selection	11874808
25227320	25227343	12	-	28162770	MV411	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.3994406442278253	[206]	[229]	3	hSpCas9	negative selection	11874809
25225730	25225753	12	-	28162770	MV411	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.16095803005730713	[274]	[258]	1	hSpCas9	negative selection	11874810
25225670	25225693	12	-	28162770	MV411	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.4028920694167544	[166]	[185]	3	hSpCas9	negative selection	11874811
25227305	25227328	12	-	28162770	MV411	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-0.681479351155742	[98]	[51]	-5	hSpCas9	negative selection	11874812
25227345	25227368	12	-	28162770	MV411	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.399068767684746	[387]	[430]	3	hSpCas9	negative selection	11874813
25227295	25227318	12	-	28162770	MONOMAC1	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-0.6564684656968511	[87]	[63]	-5	hSpCas9	negative selection	12044172
25245310	25245333	12	+	28162770	MONOMAC1	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	1.718265988365165	[56]	[214]	9	hSpCas9	negative selection	12044173
25245341	25245364	12	-	28162770	MONOMAC1	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-1.5727943586765862	[313]	[120]	-8	hSpCas9	negative selection	12044174
25227320	25227343	12	-	28162770	MONOMAC1	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.695249335640193	[138]	[257]	6	hSpCas9	negative selection	12044175
25225730	25225753	12	-	28162770	MONOMAC1	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-1.4064902126885035	[258]	[111]	-8	hSpCas9	negative selection	12044176
25225670	25225693	12	-	28162770	MONOMAC1	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.4094267228725247	[110]	[168]	4	hSpCas9	negative selection	12044177
25227305	25227328	12	-	28162770	MONOMAC1	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.2779568424431211	[54]	[25]	-7	hSpCas9	negative selection	12044178
25227345	25227368	12	-	28162770	MONOMAC1	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	-0.031158678679943375	[237]	[266]	0	hSpCas9	negative selection	12044179
25227295	25227318	12	-	28162770	OCIAML2	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	0.34433828092531793	[26]	[189]	3	hSpCas9	negative selection	12213538
25245310	25245333	12	+	28162770	OCIAML2	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.6618204707814882	[12]	[113]	6	hSpCas9	negative selection	12213539
25245341	25245364	12	-	28162770	OCIAML2	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-0.062237639605329576	[67]	[360]	0	hSpCas9	negative selection	12213540
25227320	25227343	12	-	28162770	OCIAML2	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	0.9613210569730293	[52]	[571]	8	hSpCas9	negative selection	12213541
25225730	25225753	12	-	28162770	OCIAML2	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	0.3924883683374516	[57]	[421]	4	hSpCas9	negative selection	12213542
25225670	25225693	12	-	28162770	OCIAML2	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	0.8789546125480666	[30]	[315]	8	hSpCas9	negative selection	12213543
25227305	25227328	12	-	28162770	OCIAML2	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	0.7728242116221165	[16]	[160]	7	hSpCas9	negative selection	12213544
25227345	25227368	12	-	28162770	OCIAML2	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.7503104620603744	[70]	[661]	7	hSpCas9	negative selection	12213545
25227295	25227318	12	-	28162770	OCIAML3	viability	CCAGTACATGAGGACTGGGGAGG	KRAS	ENSG00000133703	-1.4930787314733824	[68]	[73]	-7	hSpCas9	negative selection	12382904
25245310	25245333	12	+	28162770	OCIAML3	viability	CTGAATTAGCTGTATCGTCAAGG	KRAS	ENSG00000133703	0.6180428367908258	[29]	[138]	5	hSpCas9	negative selection	12382905
25245341	25245364	12	-	28162770	OCIAML3	viability	GTAGTTGGAGCTGGTGGCGTAGG	KRAS	ENSG00000133703	-0.6138161214043436	[109]	[216]	-5	hSpCas9	negative selection	12382906
25227320	25227343	12	-	28162770	OCIAML3	viability	AAGAGGAGTACAGTGCAATGAGG	KRAS	ENSG00000133703	-1.043315041687753	[70]	[103]	-6	hSpCas9	negative selection	12382907
25225730	25225753	12	-	28162770	OCIAML3	viability	GGACTCTGAAGATGTACCTATGG	KRAS	ENSG00000133703	-0.22722530965254029	[75]	[195]	-2	hSpCas9	negative selection	12382908
25225670	25225693	12	-	28162770	OCIAML3	viability	AGTAGACACAAAACAGGCTCAGG	KRAS	ENSG00000133703	-0.5642602969301111	[47]	[97]	-4	hSpCas9	negative selection	12382909
25227305	25227328	12	-	28162770	OCIAML3	viability	CAATGAGGGACCAGTACATGAGG	KRAS	ENSG00000133703	-1.1442047228846428	[40]	[55]	-7	hSpCas9	negative selection	12382910
25227345	25227368	12	-	28162770	OCIAML3	viability	TTGGATATTCTCGACACAGCAGG	KRAS	ENSG00000133703	0.6784870947586968	[94]	[458]	6	hSpCas9	negative selection	12382911