Crispr

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 55288
• Symbol: RHOT1
• Description: ras homolog family member T1

DataSource: http://genomecrispr.dkfz.de/#!/results/RHOT1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
32183242	32183265	17	+	26472758	Jiyoye	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.05379840193982932	[233]	[169]	0	hSpCas9	negative selection	136872
32192207	32192230	17	-	26472758	Jiyoye	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.3724099633451967	[41]	[40]	3	hSpCas9	negative selection	136873
32199510	32199533	17	+	26472758	Jiyoye	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.15835483489178037	[201]	[169]	1	hSpCas9	negative selection	136874
32182844	32182867	17	+	26472758	Jiyoye	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	-1.1777871192152825	[104]	[34]	-7	hSpCas9	negative selection	136875
32198957	32198980	17	-	26472758	Jiyoye	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	1.2745376129277546	[73]	[134]	7	hSpCas9	negative selection	136876
32208224	32208247	17	-	26472758	Jiyoye	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	-0.05440470371000067	[167]	[121]	0	hSpCas9	negative selection	136877
32182775	32182798	17	+	26472758	Jiyoye	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	-2.084677714823801	[269]	[47]	-8	hSpCas9	negative selection	136878
32193208	32193231	17	+	26472758	Jiyoye	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.2586528567957854	[91]	[82]	2	hSpCas9	negative selection	136879
32199526	32199549	17	+	26472758	Jiyoye	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.6411876400555256	[90]	[43]	-4	hSpCas9	negative selection	136880
32182855	32182878	17	-	26472758	Jiyoye	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.7724598455876176	[176]	[227]	5	hSpCas9	negative selection	136881
32183242	32183265	17	+	26472758	KBM7	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.34520264093941333	[581,341]	[383,167]	3	hSpCas9	negative selection	327990
32192207	32192230	17	-	26472758	KBM7	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.9825816882788847	[151,130]	[62,191]	9	hSpCas9	negative selection	327991
32199510	32199533	17	+	26472758	KBM7	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	-0.19593199519681975	[893,624]	[342,275]	-2	hSpCas9	negative selection	327992
32182844	32182867	17	+	26472758	KBM7	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	-1.5323925097776867	[218,76]	[41,5]	-8	hSpCas9	negative selection	327993
32198957	32198980	17	-	26472758	KBM7	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.19015830099529543	[435,364]	[233,195]	2	hSpCas9	negative selection	327994
32208224	32208247	17	-	26472758	KBM7	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.6287758653237002	[668,443]	[403,392]	7	hSpCas9	negative selection	327995
32182775	32182798	17	+	26472758	KBM7	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	-0.406031390192009	[333,347]	[86,152]	-4	hSpCas9	negative selection	327996
32193208	32193231	17	+	26472758	KBM7	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.7713809604853473	[462,420]	[321,383]	8	hSpCas9	negative selection	327997
32199526	32199549	17	+	26472758	KBM7	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.25420323538699174	[451,228]	[122,136]	-2	hSpCas9	negative selection	327998
32182855	32182878	17	-	26472758	KBM7	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.30268459468772835	[1041,1124]	[635,631]	3	hSpCas9	negative selection	327999
32183242	32183265	17	+	26472758	Raji	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.12412679920817588	[256]	[64]	1	hSpCas9	negative selection	519108
32192207	32192230	17	-	26472758	Raji	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.31156901390862757	[122]	[22]	-3	hSpCas9	negative selection	519109
32199510	32199533	17	+	26472758	Raji	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.41831263131170493	[473]	[146]	4	hSpCas9	negative selection	519110
32182844	32182867	17	+	26472758	Raji	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	0.633452347041187	[24]	[8]	6	hSpCas9	negative selection	519111
32198957	32198980	17	-	26472758	Raji	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.7169240588154215	[193]	[73]	6	hSpCas9	negative selection	519112
32208224	32208247	17	-	26472758	Raji	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	-0.2922239233356385	[247]	[46]	-2	hSpCas9	negative selection	519113
32182775	32182798	17	+	26472758	Raji	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	-1.998150878798758	[240]	[13]	-8	hSpCas9	negative selection	519114
32193208	32193231	17	+	26472758	Raji	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	-1.5676111662100292	[280]	[21]	-8	hSpCas9	negative selection	519115
32199526	32199549	17	+	26472758	Raji	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.4607051467831604	[254]	[42]	-4	hSpCas9	negative selection	519116
32182855	32182878	17	-	26472758	Raji	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.5217787692580919	[463]	[74]	-4	hSpCas9	negative selection	519117
32183242	32183265	17	+	26472758	K562	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.628377915759847	[304]	[397]	5	hSpCas9	negative selection	710226
32192207	32192230	17	-	26472758	K562	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.8957237232162991	[169]	[266]	7	hSpCas9	negative selection	710227
32199510	32199533	17	+	26472758	K562	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.4299317145785382	[582]	[662]	3	hSpCas9	negative selection	710228
32182844	32182867	17	+	26472758	K562	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	0.30581927233059036	[160]	[167]	2	hSpCas9	negative selection	710229
32198957	32198980	17	-	26472758	K562	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	1.3639405091059869	[410]	[892]	9	hSpCas9	negative selection	710230
32208224	32208247	17	-	26472758	K562	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	1.2610016118620508	[345]	[699]	9	hSpCas9	negative selection	710231
32182775	32182798	17	+	26472758	K562	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.7975201294324669	[350]	[514]	6	hSpCas9	negative selection	710232
32193208	32193231	17	+	26472758	K562	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.8034527633970769	[392]	[578]	6	hSpCas9	negative selection	710233
32199526	32199549	17	+	26472758	K562	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.3405437730547094	[260]	[173]	-2	hSpCas9	negative selection	710234
32182855	32182878	17	-	26472758	K562	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.16906504566307823	[785]	[745]	1	hSpCas9	negative selection	710235
32182855	32182878	17	-	26627737	DLD1	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.0665363476541363	[999]	[293,269,325]	0	hSpCas9	negative selection	796937
32183246	32183269	17	-	26627737	DLD1	viability	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.2125794719133306	[759]	[193,282,149]	-2	hSpCas9	negative selection	796938
32209365	32209388	17	+	26627737	DLD1	viability	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.36002835881703965	[1201]	[292,418,187]	-4	hSpCas9	negative selection	796939
32211121	32211144	17	-	26627737	DLD1	viability	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.07863502625091212	[197]	[85,49,41]	-1	hSpCas9	negative selection	796940
32253111	32253134	17	-	26627737	DLD1	viability	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.8143457094042308	[85]	[25,58,55]	7	hSpCas9	negative selection	796941
32182855	32182878	17	-	26627737	GBM cells	viability after 5 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.13964597164535475	[454]	[221,220]	-2	hSpCas9	negative selection	879252
32183246	32183269	17	-	26627737	GBM cells	viability after 5 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.044213428034658775	[452]	[244,255]	0	hSpCas9	negative selection	879253
32209365	32209388	17	+	26627737	GBM cells	viability after 5 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.059265949419687214	[577]	[316,277]	-1	hSpCas9	negative selection	879254
32211121	32211144	17	-	26627737	GBM cells	viability after 5 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.5276580676347549	[118]	[122,60]	7	hSpCas9	negative selection	879255
32253111	32253134	17	-	26627737	GBM cells	viability after 5 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.6456274456034536	[58]	[43,54]	8	hSpCas9	negative selection	879256
32182855	32182878	17	-	26627737	GBM cells	viability after 13 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.18396785261969717	[454]	[229,206]	-2	hSpCas9	negative selection	961567
32183246	32183269	17	-	26627737	GBM cells	viability after 13 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.011614079079129924	[452]	[293,202]	0	hSpCas9	negative selection	961568
32209365	32209388	17	+	26627737	GBM cells	viability after 13 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.39885190554102845	[577]	[308,179]	-5	hSpCas9	negative selection	961569
32211121	32211144	17	-	26627737	GBM cells	viability after 13 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.8058675377513622	[118]	[140,89]	8	hSpCas9	negative selection	961570
32253111	32253134	17	-	26627737	GBM cells	viability after 13 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-0.04156081902642139	[58]	[29,31]	0	hSpCas9	negative selection	961571
32182855	32182878	17	-	26627737	GBM cells	viability after 21 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.5397213734539625	[454]	[168,170]	-5	hSpCas9	negative selection	1043882
32183246	32183269	17	-	26627737	GBM cells	viability after 21 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.09994567334524573	[452]	[249,210]	-1	hSpCas9	negative selection	1043883
32209365	32209388	17	+	26627737	GBM cells	viability after 21 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.5113666980714996	[577]	[224,215]	-4	hSpCas9	negative selection	1043884
32211121	32211144	17	-	26627737	GBM cells	viability after 21 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	1.0896356947261026	[118]	[158,117]	9	hSpCas9	negative selection	1043885
32253111	32253134	17	-	26627737	GBM cells	viability after 21 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.7441418932492212	[58]	[60,46]	7	hSpCas9	negative selection	1043886
32182855	32182878	17	-	26627737	RPE1	viability after 9 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.10475094158931253	[1325]	[351,429]	0	hSpCas9	negative selection	1126197
32183246	32183269	17	-	26627737	RPE1	viability after 9 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.5993651380631805	[1405]	[276,307]	-4	hSpCas9	negative selection	1126198
32209365	32209388	17	+	26627737	RPE1	viability after 9 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.9894526329791771	[1646]	[360,128]	-6	hSpCas9	negative selection	1126199
32211121	32211144	17	-	26627737	RPE1	viability after 9 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.6867987227716741	[276]	[83,14]	-5	hSpCas9	negative selection	1126200
32253111	32253134	17	-	26627737	RPE1	viability after 9 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-2.397654631158674	[251]	[0,32]	-9	hSpCas9	negative selection	1126201
32182855	32182878	17	-	26627737	RPE1	viability after 12 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.10229285314372383	[1325]	[311,456]	0	hSpCas9	negative selection	1208512
32183246	32183269	17	-	26627737	RPE1	viability after 12 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.22129224833895822	[1405]	[290,358]	-1	hSpCas9	negative selection	1208513
32209365	32209388	17	+	26627737	RPE1	viability after 12 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-2.2525724421950044	[1646]	[104,79]	-8	hSpCas9	negative selection	1208514
32211121	32211144	17	-	26627737	RPE1	viability after 12 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.07603447599223245	[276]	[107,30]	0	hSpCas9	negative selection	1208515
32253111	32253134	17	-	26627737	RPE1	viability after 12 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-2.1936432410851916	[251]	[7,21]	-8	hSpCas9	negative selection	1208516
32182855	32182878	17	-	26627737	RPE1	viability after 15 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.6042744435701007	[1325]	[669,473]	4	hSpCas9	negative selection	1290827
32183246	32183269	17	-	26627737	RPE1	viability after 15 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.2264810901992821	[1405]	[314,363]	-1	hSpCas9	negative selection	1290828
32209365	32209388	17	+	26627737	RPE1	viability after 15 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.8957043977870012	[1646]	[423,82]	-5	hSpCas9	negative selection	1290829
32211121	32211144	17	-	26627737	RPE1	viability after 15 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.2647814877814505	[276]	[57,128]	1	hSpCas9	negative selection	1290830
32253111	32253134	17	-	26627737	RPE1	viability after 15 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-3.0452101378038954	[251]	[0,15]	-9	hSpCas9	negative selection	1290831
32182855	32182878	17	-	26627737	RPE1	viability after 18 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.33725861941378754	[1325]	[275,290]	-2	hSpCas9	negative selection	1373142
32183246	32183269	17	-	26627737	RPE1	viability after 18 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.25885092863094494	[1405]	[424,484]	1	hSpCas9	negative selection	1373143
32209365	32209388	17	+	26627737	RPE1	viability after 18 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.7210415164300416	[1646]	[302,233]	-4	hSpCas9	negative selection	1373144
32211121	32211144	17	-	26627737	RPE1	viability after 18 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.587182830633217	[276]	[91,133]	3	hSpCas9	negative selection	1373145
32253111	32253134	17	-	26627737	RPE1	viability after 18 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-3.4282497870173865	[251]	[0,11]	-9	hSpCas9	negative selection	1373146
32182855	32182878	17	-	26627737	HeLa	viability after 8 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	1.1055877595893466	[230]	[278,171,211]	8	hSpCas9	negative selection	1455457
32183246	32183269	17	-	26627737	HeLa	viability after 8 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.12761313350821035	[689]	[514,297,219]	1	hSpCas9	negative selection	1455458
32209365	32209388	17	+	26627737	HeLa	viability after 8 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.05695060989182732	[785]	[463,253,371]	0	hSpCas9	negative selection	1455459
32211121	32211144	17	-	26627737	HeLa	viability after 8 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.5828389859488263	[308]	[133,24,117]	-5	hSpCas9	negative selection	1455460
32253111	32253134	17	-	26627737	HeLa	viability after 8 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	2.6189023422573787	[14]	[79,7,40]	9	hSpCas9	negative selection	1455461
32182855	32182878	17	-	26627737	HeLa	viability after 12 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.9626153329531743	[230]	[129,237,182]	7	hSpCas9	negative selection	1537772
32183246	32183269	17	-	26627737	HeLa	viability after 12 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.116206948625731	[689]	[404,143,256]	-1	hSpCas9	negative selection	1537773
32209365	32209388	17	+	26627737	HeLa	viability after 12 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.07116070431050003	[785]	[308,263,457]	0	hSpCas9	negative selection	1537774
32211121	32211144	17	-	26627737	HeLa	viability after 12 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.8661367832608746	[308]	[62,31,118]	-7	hSpCas9	negative selection	1537775
32253111	32253134	17	-	26627737	HeLa	viability after 12 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	2.365940751642008	[14]	[5,32,55]	9	hSpCas9	negative selection	1537776
32182855	32182878	17	-	26627737	HeLa	viability after 15 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.028011557034598455	[230]	[107,45,124]	0	hSpCas9	negative selection	1620087
32183246	32183269	17	-	26627737	HeLa	viability after 15 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.16237328543991725	[689]	[246,303,197]	-1	hSpCas9	negative selection	1620088
32209365	32209388	17	+	26627737	HeLa	viability after 15 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.48854863601343035	[785]	[220,129,341]	-4	hSpCas9	negative selection	1620089
32211121	32211144	17	-	26627737	HeLa	viability after 15 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.3787509998957436	[308]	[121,33,136]	-3	hSpCas9	negative selection	1620090
32253111	32253134	17	-	26627737	HeLa	viability after 15 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	2.8582237282967773	[14]	[114,7,5]	9	hSpCas9	negative selection	1620091
32182855	32182878	17	-	26627737	HeLa	viability after 18 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.10172040251533965	[230]	[65,34,124]	-1	hSpCas9	negative selection	1702402
32183246	32183269	17	-	26627737	HeLa	viability after 18 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.13748658881015885	[689]	[173,251,197]	-1	hSpCas9	negative selection	1702403
32209365	32209388	17	+	26627737	HeLa	viability after 18 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.49114783018628116	[785]	[143,111,341]	-4	hSpCas9	negative selection	1702404
32211121	32211144	17	-	26627737	HeLa	viability after 18 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.39866908124609785	[308]	[80,25,136]	-3	hSpCas9	negative selection	1702405
32253111	32253134	17	-	26627737	HeLa	viability after 18 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	2.8232882674331776	[14]	[73,7,5]	9	hSpCas9	negative selection	1702406
32182855	32182878	17	-	26627737	HCT116	viability after 6 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.034368997124841205	[980]	[126,174]	0	hSpCas9	negative selection	1784717
32183246	32183269	17	-	26627737	HCT116	viability after 6 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-1.397670212933467	[1064]	[127,27]	-7	hSpCas9	negative selection	1784718
32209365	32209388	17	+	26627737	HCT116	viability after 6 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.12462891578946622	[1099]	[339,104]	0	hSpCas9	negative selection	1784719
32211121	32211144	17	-	26627737	HCT116	viability after 6 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-2.76607991902605	[621]	[34,2]	-9	hSpCas9	negative selection	1784720
32253111	32253134	17	-	26627737	HCT116	viability after 6 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-2.256423622773539	[51]	[2,0]	-8	hSpCas9	negative selection	1784721
32182855	32182878	17	-	26627737	HCT116	viability after 8 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.4325547357758317	[278]	[185,304,131]	4	hSpCas9	negative selection	1867032
32183246	32183269	17	-	26627737	HCT116	viability after 8 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.013367572496509106	[393]	[175,293,186]	0	hSpCas9	negative selection	1867033
32209365	32209388	17	+	26627737	HCT116	viability after 8 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.5989630838686961	[395]	[326,379,284]	6	hSpCas9	negative selection	1867034
32211121	32211144	17	-	26627737	HCT116	viability after 8 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.5294593664672451	[220]	[257,112,157]	5	hSpCas9	negative selection	1867035
32253111	32253134	17	-	26627737	HCT116	viability after 8 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.20921290372734624	[58]	[48,29,33]	2	hSpCas9	negative selection	1867036
32182855	32182878	17	-	26627737	HCT116	viability after 9 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.8582388062585404	[980]	[134,151]	-6	hSpCas9	negative selection	1949347
32183246	32183269	17	-	26627737	HCT116	viability after 9 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.13313442115206697	[1064]	[222,287]	-1	hSpCas9	negative selection	1949348
32209365	32209388	17	+	26627737	HCT116	viability after 9 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.4255670197903626	[1099]	[508,303]	3	hSpCas9	negative selection	1949349
32211121	32211144	17	-	26627737	HCT116	viability after 9 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-1.243530195779957	[621]	[125,25]	-7	hSpCas9	negative selection	1949350
32253111	32253134	17	-	26627737	HCT116	viability after 9 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	1.2650026101225071	[51]	[5,54]	8	hSpCas9	negative selection	1949351
32182855	32182878	17	-	26627737	HCT116	viability after 12 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.6735409561638039	[278]	[249,185,262]	6	hSpCas9	negative selection	2031662
32183246	32183269	17	-	26627737	HCT116	viability after 12 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.1324767267942238	[393]	[184,160,336]	1	hSpCas9	negative selection	2031663
32209365	32209388	17	+	26627737	HCT116	viability after 12 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.9378508735762972	[395]	[331,486,384]	7	hSpCas9	negative selection	2031664
32211121	32211144	17	-	26627737	HCT116	viability after 12 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.4745326098300003	[220]	[252,116,104]	4	hSpCas9	negative selection	2031665
32253111	32253134	17	-	26627737	HCT116	viability after 12 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-0.22328983327883867	[58]	[7,54,18]	-2	hSpCas9	negative selection	2031666
32182855	32182878	17	-	26627737	HCT116	viability after 15 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.3694540884553478	[278]	[270,252,105]	3	hSpCas9	negative selection	2113977
32183246	32183269	17	-	26627737	HCT116	viability after 15 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.08119379147052319	[393]	[290,160,271]	0	hSpCas9	negative selection	2113978
32209365	32209388	17	+	26627737	HCT116	viability after 15 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.8612610212818219	[395]	[401,334,499]	7	hSpCas9	negative selection	2113979
32211121	32211144	17	-	26627737	HCT116	viability after 15 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	1.0472769456338893	[220]	[344,276,175]	8	hSpCas9	negative selection	2113980
32253111	32253134	17	-	26627737	HCT116	viability after 15 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.32863296533839614	[58]	[28,53,42]	3	hSpCas9	negative selection	2113981
32182855	32182878	17	-	26627737	HCT116	viability after 18 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.9088945865185236	[278]	[410,177,171]	7	hSpCas9	negative selection	2196292
32183246	32183269	17	-	26627737	HCT116	viability after 18 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.23037294414026946	[393]	[215,106,167]	-2	hSpCas9	negative selection	2196293
32209365	32209388	17	+	26627737	HCT116	viability after 18 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	1.5200416565606647	[395]	[596,299,776]	9	hSpCas9	negative selection	2196294
32211121	32211144	17	-	26627737	HCT116	viability after 18 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.9390149480422267	[220]	[187,318,94]	7	hSpCas9	negative selection	2196295
32253111	32253134	17	-	26627737	HCT116	viability after 18 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.5743805523429379	[58]	[27,51,46]	5	hSpCas9	negative selection	2196296
32147168	32147191	17	-	25494202	A375	resistance to PLX-4720 (puromycin)	CGCCTGTAATCCCAGTTACTTGG	RHOT1	ENSG00000126858	-1.4499740674906352	[80,484]	[11,33]	-8	dCas9-VP64	positive selection	2386055
32196304	32196327	17	-	25494202	A375	resistance to PLX-4720 (puromycin)	CCCAGCTACTTGGCAGGCTGAAG	RHOT1	ENSG00000126858	2.180224224283918	[67,41]	[97,7]	7	dCas9-VP64	positive selection	2392969
32178450	32178473	17	-	25494202	A375	resistance to PLX-4720 (puromycin)	CGTCTGCAATCCCAGCACCTCGG	RHOT1	ENSG00000126858	4.45375407688863	[141,327]	[34,2339]	9	dCas9-VP64	positive selection	2408151
32192922	32192945	17	-	25494202	A375	resistance to PLX-4720 (puromycin)	GCCTGTAATCCCAGCACTTTGGG	RHOT1	ENSG00000126858	0.14000166748158183	[525,123]	[99,52]	0	dCas9-VP64	positive selection	2444596
32154982	32155005	17	+	25494202	A375	resistance to PLX-4720 (puromycin)	GCTGAGGCAGGAGGATCACCTGG	RHOT1	ENSG00000126858	1.7615688706840174	[50,85]	[78,20]	6	dCas9-VP64	positive selection	2468235
32163472	32163495	17	-	25494202	A375	resistance to PLX-4720 (puromycin)	TCCCAAAATGCTGGGATTACCAG	RHOT1	ENSG00000126858	0.20161513672590614	[46,60]	[10,15]	1	dCas9-VP64	positive selection	2475351
32147168	32147191	17	-	25494202	A375	resistance to PLX-4720 (zeocin)	CGCCTGTAATCCCAGTTACTTGG	RHOT1	ENSG00000126858	0.5129370309291919	[140,116]	[29,88]	4	dCas9-VP64	positive selection	2481310
32196304	32196327	17	-	25494202	A375	resistance to PLX-4720 (zeocin)	CCCAGCTACTTGGCAGGCTGAAG	RHOT1	ENSG00000126858	0.5215654761973469	[134,134]	[72,52]	4	dCas9-VP64	positive selection	2488224
32178450	32178473	17	-	25494202	A375	resistance to PLX-4720 (zeocin)	CGTCTGCAATCCCAGCACCTCGG	RHOT1	ENSG00000126858	-1.0967727414741575	[288,207]	[42,31]	-9	dCas9-VP64	positive selection	2503406
32192922	32192945	17	-	25494202	A375	resistance to PLX-4720 (zeocin)	GCCTGTAATCCCAGCACTTTGGG	RHOT1	ENSG00000126858	2.3170246663226814	[189,235]	[324,366]	8	dCas9-VP64	positive selection	2539851
32154982	32155005	17	+	25494202	A375	resistance to PLX-4720 (zeocin)	GCTGAGGCAGGAGGATCACCTGG	RHOT1	ENSG00000126858	0.5700051099221695	[73,76]	[29,41]	4	dCas9-VP64	positive selection	2563490
32163472	32163495	17	-	25494202	A375	resistance to PLX-4720 (zeocin)	TCCCAAAATGCTGGGATTACCAG	RHOT1	ENSG00000126858	-0.42275240861415275	[53,301]	[32,50]	-4	dCas9-VP64	positive selection	2570606
32198957	32198980	17	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	-0.2009080156085647	[4,4]	[3,2]	-3	hSpCas9	positive selection	3016803
32183252	32183275	17	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GTACTGCCCAGAGGAGAAGGAGG	RHOT1	ENSG00000126858	0.09426363486179147	[3,3]	[2,3]	2	hSpCas9	positive selection	3016804
32183246	32183269	17	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.4271096833696397	[3,3]	[2,1]	-6	hSpCas9	positive selection	3016805
32198957	32198980	17	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.317904432463473	[4,4]	[3,4]	5	hSpCas9	positive selection	3084661
32183252	32183275	17	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GTACTGCCCAGAGGAGAAGGAGG	RHOT1	ENSG00000126858	-0.9089239194192144	[3,3]	[1,0]	-9	hSpCas9	positive selection	3084662
32183246	32183269	17	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.26088872823093245	[3,3]	[4,0]	-4	hSpCas9	positive selection	3084663
32175987	32176010	17	-	27383988	293T	resistance to West Nile virus (flavivirus)	AATAGAATGCTTGTTGTTAACGG	RHOT1	ENSG00000126858	-1.1856172844028932	[347,347]	[0,0]	-7	hSpCas9	positive selection	3389812
32175994	32176017	17	+	27383988	293T	resistance to West Nile virus (flavivirus)	CAACAAGCATTCTATTGATAAGG	RHOT1	ENSG00000126858	2.169863370195496	[33,33]	[0,0]	7	hSpCas9	positive selection	3389813
32198957	32198980	17	-	26780180	HT29	viability (Avana library 4 designs)	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	1.06948468128649	[]	[]	4	hSpCas9	negative selection	3455149
32183251	32183274	17	+	26780180	HT29	viability (Avana library 4 designs)	GTACTGCCCAGAGGAGAAGGAGG	RHOT1	ENSG00000126858	1.87720098292537	[]	[]	7	hSpCas9	negative selection	3455150
32183246	32183269	17	-	26780180	HT29	viability (Avana library 4 designs)	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	1.73298097411488	[]	[]	6	hSpCas9	negative selection	3455151
32198957	32198980	17	-	26780180	A375	viability (Avana lentiCRISPRv2)	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	-2.92822700598622	[]	[]	-4	hSpCas9	negative selection	3552988
32183251	32183274	17	+	26780180	A375	viability (Avana lentiCRISPRv2)	GTACTGCCCAGAGGAGAAGGAGG	RHOT1	ENSG00000126858	-0.859246770140851	[]	[]	-1	hSpCas9	negative selection	3552989
32183246	32183269	17	-	26780180	A375	viability (Avana lentiCRISPRv2)	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.771662264573774	[]	[]	1	hSpCas9	negative selection	3552990
32183223	32183246	17	+	26780180	A375	viability (Avana lentiCRISPRv2)	AAGCTGTTCTTCATCCTACAGGG	RHOT1	ENSG00000126858	0.841756137923591	[]	[]	1	hSpCas9	negative selection	3552991
32193209	32193232	17	+	26780180	A375	viability (Avana lentiCRISPRv2)	GTGATGGTGTGGCTGACAGTGGG	RHOT1	ENSG00000126858	3.37739511999231	[]	[]	5	hSpCas9	negative selection	3552992
32198957	32198980	17	-	26780180	A375	viability (Avana lentiGuide)	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	-0.406497320783351	[]	[]	0	hSpCas9	negative selection	3661647
32183251	32183274	17	+	26780180	A375	viability (Avana lentiGuide)	GTACTGCCCAGAGGAGAAGGAGG	RHOT1	ENSG00000126858	3.89186582581142	[]	[]	4	hSpCas9	negative selection	3661648
32183246	32183269	17	-	26780180	A375	viability (Avana lentiGuide)	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-2.13958188883084	[]	[]	-4	hSpCas9	negative selection	3661649
32183223	32183246	17	+	26780180	A375	viability (Avana lentiGuide)	AAGCTGTTCTTCATCCTACAGGG	RHOT1	ENSG00000126858	-3.58668279034139	[]	[]	-6	hSpCas9	negative selection	3661650
32193209	32193232	17	+	26780180	A375	viability (Avana lentiGuide)	GTGATGGTGTGGCTGACAGTGGG	RHOT1	ENSG00000126858	7.75333673416253	[]	[]	6	hSpCas9	negative selection	3661651
32198957	32198980	17	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	-0.2701848330638541	[6]	[6,4]	-4	hSpCas9	positive selection	3770254
32183251	32183274	17	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GTACTGCCCAGAGGAGAAGGAGG	RHOT1	ENSG00000126858	0.012648714954268836	[4]	[3,4]	0	hSpCas9	positive selection	3770255
32183246	32183269	17	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.058551902258965685	[5]	[5,3]	-1	hSpCas9	positive selection	3770256
32183223	32183246	17	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AAGCTGTTCTTCATCCTACAGGG	RHOT1	ENSG00000126858	0.036712976625426996	[5]	[6,5]	0	hSpCas9	positive selection	3770257
32193209	32193232	17	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GTGATGGTGTGGCTGACAGTGGG	RHOT1	ENSG00000126858	0.09877725333557912	[5]	[5,5]	2	hSpCas9	positive selection	3770258
32198957	32198980	17	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.10019833349789525	[6]	[6,5]	2	hSpCas9	positive selection	3878861
32183251	32183274	17	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GTACTGCCCAGAGGAGAAGGAGG	RHOT1	ENSG00000126858	-0.0807517782739123	[4]	[2,5]	-1	hSpCas9	positive selection	3878862
32183246	32183269	17	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.2330226675341517	[5]	[4,5]	6	hSpCas9	positive selection	3878863
32183223	32183246	17	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AAGCTGTTCTTCATCCTACAGGG	RHOT1	ENSG00000126858	0.2666670381920735	[5]	[4,5]	7	hSpCas9	positive selection	3878864
32193209	32193232	17	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GTGATGGTGTGGCTGACAGTGGG	RHOT1	ENSG00000126858	0.004936557671281594	[6]	[4,5]	0	hSpCas9	positive selection	3878865
32175341	32175364	17	+	26780180	A375	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	1.24038791134136	[]	[]	6	hSpCas9	negative selection	3988996
32171056	32171079	17	+	26780180	A375	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.966177669253766	[]	[]	4	hSpCas9	negative selection	3988997
32175341	32175364	17	+	26780180	HT29	viability (GeCKOv2 library)	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	1.89736467691518	[]	[]	9	hSpCas9	negative selection	4096962
32171056	32171079	17	+	26780180	HT29	viability (GeCKOv2 library)	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.433014183820434	[]	[]	1	hSpCas9	negative selection	4096963
32171056	32171079	17	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.3967148847347542	[3]	[4,3]	7	hSpCas9	positive selection	4294833
32175341	32175364	17	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.4931667147973164	[2]	[1,0]	-8	hSpCas9	positive selection	4294835
32194075	32194097	17	-	27760321	OCIAML3	viability	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	-0.5716770292701301	[152,115]	[64,76]	-5	hSpCas9	negative selection	4434587
32175999	32176021	17	+	27760321	OCIAML3	viability	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	-0.4729716964712898	[63,51]	[25,40]	-5	hSpCas9	negative selection	4434588
32182855	32182877	17	-	27760321	OCIAML3	viability	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.24223551520339956	[432,313]	[286,413]	3	hSpCas9	negative selection	4434589
32183223	32183245	17	+	27760321	OCIAML3	viability	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-0.7996036324419398	[301,242]	[129,110]	-6	hSpCas9	negative selection	4434590
32192207	32192229	17	-	27760321	OCIAML3	viability	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-1.0450742974885996	[18,18]	[11,0]	-7	hSpCas9	negative selection	4434591
32194075	32194097	17	-	27760321	OCIAML2	viability	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	0.8937022440310802	[152,115]	[184,220]	9	hSpCas9	negative selection	4518050
32175999	32176021	17	+	27760321	OCIAML2	viability	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	-0.409677342272841	[63,51]	[35,33]	-5	hSpCas9	negative selection	4518051
32182855	32182877	17	-	27760321	OCIAML2	viability	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.22124561037920829	[432,313]	[244,271]	-3	hSpCas9	negative selection	4518052
32183223	32183245	17	+	27760321	OCIAML2	viability	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-0.14395662550491028	[301,242]	[203,190]	-2	hSpCas9	negative selection	4518053
32192207	32192229	17	-	27760321	OCIAML2	viability	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.5829862628388511	[18,18]	[8,11]	-6	hSpCas9	negative selection	4518054
32194075	32194097	17	-	27760321	MV411	viability	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	1.5443489233286067	[152,115]	[263,245]	9	hSpCas9	negative selection	4601513
32175999	32176021	17	+	27760321	MV411	viability	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	0.4167685649417834	[63,51]	[83,0]	3	hSpCas9	negative selection	4601514
32182855	32182877	17	-	27760321	MV411	viability	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.44103680879574025	[432,313]	[246,457]	3	hSpCas9	negative selection	4601515
32183223	32183245	17	+	27760321	MV411	viability	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-2.45668846647405	[301,242]	[0,79]	-9	hSpCas9	negative selection	4601516
32192207	32192229	17	-	27760321	MV411	viability	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-3.6529790123215893	[18,18]	[0,0]	-9	hSpCas9	negative selection	4601517
32194075	32194097	17	-	27760321	HL60	viability	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	0.566642544517693	[152,115]	[191,128]	6	hSpCas9	negative selection	4684976
32175999	32176021	17	+	27760321	HL60	viability	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	1.017302883004534	[63,51]	[58,133]	9	hSpCas9	negative selection	4684977
32182855	32182877	17	-	27760321	HL60	viability	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-1.0377265253776762	[432,313]	[168,123]	-7	hSpCas9	negative selection	4684978
32183223	32183245	17	+	27760321	HL60	viability	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	0.08341277049499496	[301,242]	[135,338]	1	hSpCas9	negative selection	4684979
32192207	32192229	17	-	27760321	HL60	viability	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.14275507011226463	[18,18]	[0,27]	-2	hSpCas9	negative selection	4684980
32194075	32194097	17	-	27760321	HT1080	viability	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	-1.6065941839809819	[115,90]	[24,0]	-5	hSpCas9	negative selection	4768439
32175999	32176021	17	+	27760321	HT1080	viability	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	0.3930915404452766	[51,40]	[20,27]	2	hSpCas9	negative selection	4768440
32182855	32182877	17	-	27760321	HT1080	viability	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-6.798863530417789	[313,244]	[0,0]	-9	hSpCas9	negative selection	4768441
32183223	32183245	17	+	27760321	HT1080	viability	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	0.32012282782818036	[242,189]	[79,137]	1	hSpCas9	negative selection	4768442
32192207	32192229	17	-	27760321	HT1080	viability	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	2.247474412330948	[18,14]	[35,27]	9	hSpCas9	negative selection	4768443
32194075	32194097	17	-	27760321	HT29	viability after 7 days	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	0.45593128883802914	[115,90]	[142,61,331]	7	hSpCas9	negative selection	4851902
32175999	32176021	17	+	27760321	HT29	viability after 7 days	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	0.23300086527049757	[51,40]	[89,64,58]	3	hSpCas9	negative selection	4851903
32182855	32182877	17	-	27760321	HT29	viability after 7 days	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.2903004400562782	[313,244]	[517,437,374]	4	hSpCas9	negative selection	4851904
32183223	32183245	17	+	27760321	HT29	viability after 7 days	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	0.16876040747108834	[242,189]	[495,172,295]	2	hSpCas9	negative selection	4851905
32192207	32192229	17	-	27760321	HT29	viability after 7 days	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-2.8259692915922807	[18,14]	[1,0,5]	-9	hSpCas9	negative selection	4851906
32194075	32194097	17	-	27760321	HT29	viability after 25 days	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	0.24126775499679165	[115,90]	[44,251,126]	2	hSpCas9	negative selection	4935365
32175999	32176021	17	+	27760321	HT29	viability after 25 days	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	-0.9985006884331074	[51,40]	[45,10,20]	-6	hSpCas9	negative selection	4935366
32182855	32182877	17	-	27760321	HT29	viability after 25 days	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.005436631496613775	[313,244]	[378,220,361]	0	hSpCas9	negative selection	4935367
32183223	32183245	17	+	27760321	HT29	viability after 25 days	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-0.6482769756608515	[242,189]	[188,205,66]	-5	hSpCas9	negative selection	4935368
32192207	32192229	17	-	27760321	HT29	viability after 25 days	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-1.240500122785205	[18,14]	[0,16,6]	-7	hSpCas9	negative selection	4935369
32194075	32194097	17	-	27760321	HT29	viability after 22 days	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	-0.9156701824315272	[115,90]	[35,99,54]	-6	hSpCas9	negative selection	5018828
32175999	32176021	17	+	27760321	HT29	viability after 22 days	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	-0.5124758457948486	[51,40]	[11,44,56]	-5	hSpCas9	negative selection	5018829
32182855	32182877	17	-	27760321	HT29	viability after 22 days	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.11653635229692541	[313,244]	[437,311,297]	1	hSpCas9	negative selection	5018830
32183223	32183245	17	+	27760321	HT29	viability after 22 days	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-1.6832293494073316	[242,189]	[72,78,81]	-7	hSpCas9	negative selection	5018831
32192207	32192229	17	-	27760321	HT29	viability after 22 days	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-3.569527416328749	[18,14]	[0,0,2]	-9	hSpCas9	negative selection	5018832
32194075	32194097	17	-	27760321	HT29	viability after 19 days	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	-0.591332236091727	[115,90]	[141,38,61]	-5	hSpCas9	negative selection	5102291
32175999	32176021	17	+	27760321	HT29	viability after 19 days	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	0.9337257514138413	[51,40]	[12,3,271]	8	hSpCas9	negative selection	5102292
32182855	32182877	17	-	27760321	HT29	viability after 19 days	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.28377668982015536	[313,244]	[306,270,215]	-3	hSpCas9	negative selection	5102293
32183223	32183245	17	+	27760321	HT29	viability after 19 days	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-1.4909720065784862	[242,189]	[193,37,44]	-7	hSpCas9	negative selection	5102294
32192207	32192229	17	-	27760321	HT29	viability after 19 days	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-4.28183196305005	[18,14]	[0,0,0]	-9	hSpCas9	negative selection	5102295
32194075	32194097	17	-	27760321	HT29	viability after 16 days	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	0.3885422423213494	[115,90]	[215,149,94]	4	hSpCas9	negative selection	5185754
32175999	32176021	17	+	27760321	HT29	viability after 16 days	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	-2.4871226258432917	[51,40]	[0,11,13]	-8	hSpCas9	negative selection	5185755
32182855	32182877	17	-	27760321	HT29	viability after 16 days	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.09872366197010624	[313,244]	[198,359,431]	1	hSpCas9	negative selection	5185756
32183223	32183245	17	+	27760321	HT29	viability after 16 days	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-1.4724476758760672	[242,189]	[110,91,60]	-8	hSpCas9	negative selection	5185757
32192207	32192229	17	-	27760321	HT29	viability after 16 days	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.12011468987740148	[18,14]	[32,28,0]	1	hSpCas9	negative selection	5185758
32194075	32194097	17	-	27760321	HT29	viability after 13 days	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	-0.25697704819728084	[115,90]	[70,85,141]	-3	hSpCas9	negative selection	5269217
32175999	32176021	17	+	27760321	HT29	viability after 13 days	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	-0.6728957801977584	[51,40]	[32,24,42]	-6	hSpCas9	negative selection	5269218
32182855	32182877	17	-	27760321	HT29	viability after 13 days	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.3383900281555154	[313,244]	[405,314,502]	4	hSpCas9	negative selection	5269219
32183223	32183245	17	+	27760321	HT29	viability after 13 days	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-1.1497514332869838	[242,189]	[64,114,156]	-7	hSpCas9	negative selection	5269220
32192207	32192229	17	-	27760321	HT29	viability after 13 days	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.4543080533665167	[18,14]	[33,8,0]	-5	hSpCas9	negative selection	5269221
32194075	32194097	17	-	27760321	HT29	viability after 10 days	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	-0.016658330468013016	[115,90]	[183,55,140]	0	hSpCas9	negative selection	5352680
32175999	32176021	17	+	27760321	HT29	viability after 10 days	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	-1.089434156745297	[51,40]	[7,57,16]	-8	hSpCas9	negative selection	5352681
32182855	32182877	17	-	27760321	HT29	viability after 10 days	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.13673823016426245	[313,244]	[243,487,420]	1	hSpCas9	negative selection	5352682
32183223	32183245	17	+	27760321	HT29	viability after 10 days	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-0.8581947854577225	[242,189]	[60,140,242]	-7	hSpCas9	negative selection	5352683
32192207	32192229	17	-	27760321	HT29	viability after 10 days	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.0762205219535863	[18,14]	[0,59,0]	-1	hSpCas9	negative selection	5352684
32194075	32194097	17	-	27760321	MOLM13	viability	ATATTCAGGTGTCAAATCCAGG	RHOT1	ENSG00000126858	0.027871282363628236	[152,115]	[59,90]	0	hSpCas9	negative selection	5436143
32175999	32176021	17	+	27760321	MOLM13	viability	AGCATTCTATTGATAAGGTAGG	RHOT1	ENSG00000126858	0.07014390428085049	[63,51]	[51,18]	0	hSpCas9	negative selection	5436144
32182855	32182877	17	-	27760321	MOLM13	viability	ACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	1.58609760479832	[432,313]	[1157,173]	9	hSpCas9	negative selection	5436145
32183223	32183245	17	+	27760321	MOLM13	viability	AAGCTGTTCTTCATCCTACAGG	RHOT1	ENSG00000126858	-2.1509416288703718	[301,242]	[21,44]	-8	hSpCas9	negative selection	5436146
32192207	32192229	17	-	27760321	MOLM13	viability	TAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-3.426922199493994	[18,18]	[0,0]	-9	hSpCas9	negative selection	5436147
32171056	32171079	17	+	27260156	BXPC3	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.35769047516170394	[849]	[962,992,1266,1384]	7	hSpCas9	negative selection	5567293
32175341	32175364	17	+	27260156	BXPC3	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.015696861815015728	[211]	[190,175,234,287]	0	hSpCas9	negative selection	5567295
32171056	32171079	17	+	27260156	A673	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.18205511165141652	[849]	[1040,1156,1110,1315]	4	hSpCas9	negative selection	5688544
32175341	32175364	17	+	27260156	A673	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.08464860757502546	[211]	[307,207,221,229]	-2	hSpCas9	negative selection	5688546
32171056	32171079	17	+	27260156	A375	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.31091221669633107	[849]	[1148,1465,1204,1143]	4	hSpCas9	negative selection	5809795
32175341	32175364	17	+	27260156	A375	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.17637620656422004	[211]	[199,143,356,169]	-3	hSpCas9	negative selection	5809797
32171056	32171079	17	+	27260156	COLO741	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.1234301425480836	[849]	[1257,1240,1062]	2	hSpCas9	negative selection	5931046
32175341	32175364	17	+	27260156	COLO741	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.18816885604341516	[211]	[217,281,212]	-4	hSpCas9	negative selection	5931048
32171056	32171079	17	+	27260156	CAL120	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.44868793144678765	[849]	[1719,1333,1319]	6	hSpCas9	negative selection	6052297
32175341	32175364	17	+	27260156	CAL120	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.6786055684467833	[211]	[348,452,452]	8	hSpCas9	negative selection	6052299
32171056	32171079	17	+	27260156	CADOES1	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.2258830507392512	[849]	[853,818,898,1133]	4	hSpCas9	negative selection	6173548
32175341	32175364	17	+	27260156	CADOES1	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.4187370068351528	[211]	[200,224,347,280]	7	hSpCas9	negative selection	6173550
32171056	32171079	17	+	27260156	EWS502	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.3454779728301215	[849]	[1107,1116,1071,1288]	6	hSpCas9	negative selection	6294799
32175341	32175364	17	+	27260156	EWS502	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.11591497434164766	[211]	[133,241,222,238]	-2	hSpCas9	negative selection	6294801
32171056	32171079	17	+	27260156	EW8	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.39143210286206587	[849]	[1053,791,865,921]	7	hSpCas9	negative selection	6416050
32175341	32175364	17	+	27260156	EW8	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.022684151490485638	[211]	[223,138,84,247]	0	hSpCas9	negative selection	6416052
32171056	32171079	17	+	27260156	CORL105	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.19350010846166465	[849]	[1124,1208,1158,1059]	3	hSpCas9	negative selection	6537301
32175341	32175364	17	+	27260156	CORL105	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.0037981293710582786	[211]	[379,192,228,195]	0	hSpCas9	negative selection	6537303
32171056	32171079	17	+	27260156	HS294T	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.6330178954095712	[849]	[394,704,408,536]	8	hSpCas9	negative selection	6658552
32175341	32175364	17	+	27260156	HS294T	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.15203700440422152	[211]	[99,60,127,71]	2	hSpCas9	negative selection	6658554
32171056	32171079	17	+	27260156	HCC44	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.4835958005330957	[849]	[1598,1305,1224,1246]	7	hSpCas9	negative selection	6779803
32175341	32175364	17	+	27260156	HCC44	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.3610898156975809	[211]	[131,354,221,476]	6	hSpCas9	negative selection	6779805
32171056	32171079	17	+	27260156	G402	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.2759220733994513	[849]	[1804,1020,1258,1010]	3	hSpCas9	negative selection	6901054
32175341	32175364	17	+	27260156	G402	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.312689551522911	[211]	[293,298,222,455]	4	hSpCas9	negative selection	6901056
32171056	32171079	17	+	27260156	L33	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.3058779769288279	[849]	[1180,368,620,1128]	6	hSpCas9	negative selection	7022305
32175341	32175364	17	+	27260156	L33	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.1343350280291029	[211]	[176,122,141,228]	3	hSpCas9	negative selection	7022307
32171056	32171079	17	+	27260156	K562	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.021744258353747825	[849]	[934,736,849,572]	0	hSpCas9	negative selection	7143556
32175341	32175364	17	+	27260156	K562	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.043769914230531004	[211]	[154,274,208,142]	0	hSpCas9	negative selection	7143558
32171056	32171079	17	+	27260156	HT29	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.3160294993781231	[849]	[1503,1443,1163,1079]	6	hSpCas9	negative selection	7264807
32175341	32175364	17	+	27260156	HT29	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.3385396907522251	[211]	[175,267,257,124]	-6	hSpCas9	negative selection	7264809
32171056	32171079	17	+	27260156	MHHES1	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.4175907798313373	[849]	[1426,1314,1467,970]	7	hSpCas9	negative selection	7386058
32175341	32175364	17	+	27260156	MHHES1	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.041524107569458124	[211]	[208,243,326,158]	0	hSpCas9	negative selection	7386060
32171056	32171079	17	+	27260156	MEWO	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.20020092824702818	[849]	[994,785,975]	4	hSpCas9	negative selection	7507309
32175341	32175364	17	+	27260156	MEWO	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.07016624280754491	[211]	[302,153,131]	-1	hSpCas9	negative selection	7507311
32171056	32171079	17	+	27260156	LNCAPCLONEFGC	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.42384174255907403	[849]	[1195,768,1138,1056]	6	hSpCas9	negative selection	7628560
32175341	32175364	17	+	27260156	LNCAPCLONEFGC	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.12389544325176893	[211]	[122,223,157,194]	-2	hSpCas9	negative selection	7628562
32171056	32171079	17	+	27260156	PANC0327	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.05412310084145511	[849]	[808,919,751,638]	0	hSpCas9	negative selection	7749811
32175341	32175364	17	+	27260156	PANC0327	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.2932881787771753	[211]	[320,247,115,242]	4	hSpCas9	negative selection	7749813
32171056	32171079	17	+	27260156	NCIH2009	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.7225883724078043	[849]	[1987,1645,2252]	8	hSpCas9	negative selection	7871062
32175341	32175364	17	+	27260156	NCIH2009	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.30960346589046683	[211]	[550,312,273]	4	hSpCas9	negative selection	7871064
32171056	32171079	17	+	27260156	NCIH1373	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.6155800001678996	[849]	[1461,1464,1477,373]	6	hSpCas9	negative selection	7992313
32175341	32175364	17	+	27260156	NCIH1373	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.2086137689991301	[211]	[250,69,352,42]	-2	hSpCas9	negative selection	7992315
32171056	32171079	17	+	27260156	PATU8902	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.1347537296882344	[849]	[1957,1008,1018,1260]	1	hSpCas9	negative selection	8113564
32175341	32175364	17	+	27260156	PATU8902	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.3537312460418147	[211]	[628,89,118,90]	-4	hSpCas9	negative selection	8113566
32171056	32171079	17	+	27260156	PANC1	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.1849023179352688	[849]	[888,904,1007,563]	3	hSpCas9	negative selection	8234815
32175341	32175364	17	+	27260156	PANC1	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.02586406451396628	[211]	[168,131,240,163]	0	hSpCas9	negative selection	8234817
32171056	32171079	17	+	27260156	PANC0813	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.2653890445349113	[849]	[1313,1590,902,1009]	4	hSpCas9	negative selection	8356066
32175341	32175364	17	+	27260156	PANC0813	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.3923202657207654	[211]	[237,149,183,190]	-6	hSpCas9	negative selection	8356068
32171056	32171079	17	+	27260156	RDES	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.397118661520632	[849]	[1057,1336,1256,1538]	6	hSpCas9	negative selection	8477317
32175341	32175364	17	+	27260156	RDES	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.080561432898834	[211]	[165,226,220,330]	-1	hSpCas9	negative selection	8477319
32171056	32171079	17	+	27260156	PC3	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.030804076530470015	[849]	[626,821,1309,887]	0	hSpCas9	negative selection	8598568
32175341	32175364	17	+	27260156	PC3	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.08704550646550979	[211]	[174,169,269,193]	-2	hSpCas9	negative selection	8598570
32171056	32171079	17	+	27260156	PATU8988T	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.25925836711605654	[849]	[821,1248,903,982]	4	hSpCas9	negative selection	8719819
32175341	32175364	17	+	27260156	PATU8988T	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.18291361744356804	[211]	[234,404,99,205]	3	hSpCas9	negative selection	8719821
32171056	32171079	17	+	27260156	T47D	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.2695710188990591	[849]	[1278,1068,1217,1219]	6	hSpCas9	negative selection	8841070
32175341	32175364	17	+	27260156	T47D	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.056356899198612975	[211]	[180,219,272,277]	-1	hSpCas9	negative selection	8841072
32171056	32171079	17	+	27260156	SU8686	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.12320847454647765	[849]	[877,803,949,1078]	2	hSpCas9	negative selection	8962321
32175341	32175364	17	+	27260156	SU8686	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.19120072372076724	[211]	[226,220,243,275]	3	hSpCas9	negative selection	8962323
32171056	32171079	17	+	27260156	SKES1	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.07483775718379404	[849]	[1009,968,911,717]	1	hSpCas9	negative selection	9083572
32175341	32175364	17	+	27260156	SKES1	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	-0.10557727791764937	[211]	[232,232,168,162]	-2	hSpCas9	negative selection	9083574
32171056	32171079	17	+	27260156	TOV112D	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.037106579071942924	[849]	[859,1138,765,793]	0	hSpCas9	negative selection	9204823
32175341	32175364	17	+	27260156	TOV112D	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.14980955911031602	[211]	[312,278,273,96]	3	hSpCas9	negative selection	9204825
32171056	32171079	17	+	27260156	TC71	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.15230084048354608	[849]	[789,887,1224,1153]	4	hSpCas9	negative selection	9326074
32175341	32175364	17	+	27260156	TC71	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.0808223852146921	[211]	[296,266,205,187]	2	hSpCas9	negative selection	9326076
32171056	32171079	17	+	27260156	TC32	viability	GACATCACTGATTATGTCTCTGG	RHOT1	ENSG00000126858	0.20123398646155877	[849]	[668,783,1456,882]	3	hSpCas9	negative selection	9447325
32175341	32175364	17	+	27260156	TC32	viability	ACTTCATCAAGAAATATCTCAGG	RHOT1	ENSG00000126858	0.10939918522940917	[211]	[182,171,310,217]	2	hSpCas9	negative selection	9447327
32182855	32182878	17	-	27869803	HPAFII	viability after 27 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.40977717562129456	[330]	[252,212,NaN]	3	hSpCas9	negative selection	9559955
32183246	32183269	17	-	27869803	HPAFII	viability after 27 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.24399796618035158	[376]	[138,190,NaN]	-2	hSpCas9	negative selection	9559956
32209365	32209388	17	+	27869803	HPAFII	viability after 27 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.8271890836014703	[448]	[438,401,NaN]	6	hSpCas9	negative selection	9559957
32211121	32211144	17	-	27869803	HPAFII	viability after 27 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.5114966898824718	[208]	[88,212,NaN]	4	hSpCas9	negative selection	9559958
32253111	32253134	17	-	27869803	HPAFII	viability after 27 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.22819804301765378	[61]	[59,19,NaN]	1	hSpCas9	negative selection	9559959
32182855	32182878	17	-	27869803	HPAFII	viability after 15 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.16702627923265756	[330]	[391,403,NaN]	2	hSpCas9	negative selection	9642404
32183246	32183269	17	-	27869803	HPAFII	viability after 15 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.15567214624332354	[376]	[499,278,NaN]	-2	hSpCas9	negative selection	9642405
32209365	32209388	17	+	27869803	HPAFII	viability after 15 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.24062094950582613	[448]	[616,540,NaN]	2	hSpCas9	negative selection	9642406
32211121	32211144	17	-	27869803	HPAFII	viability after 15 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.09336640755529085	[208]	[130,259,NaN]	-1	hSpCas9	negative selection	9642407
32253111	32253134	17	-	27869803	HPAFII	viability after 15 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.7924083334761314	[61]	[181,73,NaN]	8	hSpCas9	negative selection	9642408
32182855	32182878	17	-	27869803	ASPC1	viability after 36 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.5018574509575786	[1067]	[587,667,368]	5	hSpCas9	negative selection	9724853
32183246	32183269	17	-	27869803	ASPC1	viability after 36 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	0.1882085690755939	[1322]	[428,889,299]	1	hSpCas9	negative selection	9724854
32209365	32209388	17	+	27869803	ASPC1	viability after 36 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.3933697300651029	[1444]	[331,395,406]	-4	hSpCas9	negative selection	9724855
32211121	32211144	17	-	27869803	ASPC1	viability after 36 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	-0.7677760734462081	[432]	[64,33,147]	-6	hSpCas9	negative selection	9724856
32253111	32253134	17	-	27869803	ASPC1	viability after 36 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.42525230630644595	[144]	[59,97,48]	4	hSpCas9	negative selection	9724857
32182855	32182878	17	-	27869803	PATU8988S	viability after 35 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.6795935298212132	[772]	[357,29,NaN]	-6	hSpCas9	negative selection	9807302
32183246	32183269	17	-	27869803	PATU8988S	viability after 35 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.49480734243996793	[907]	[267,250,NaN]	-5	hSpCas9	negative selection	9807303
32209365	32209388	17	+	27869803	PATU8988S	viability after 35 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.5023950952747352	[1119]	[288,347,NaN]	-5	hSpCas9	negative selection	9807304
32211121	32211144	17	-	27869803	PATU8988S	viability after 35 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.9033764941716713	[344]	[213,305,NaN]	8	hSpCas9	negative selection	9807305
32253111	32253134	17	-	27869803	PATU8988S	viability after 35 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.36080010229968784	[199]	[75,130,NaN]	4	hSpCas9	negative selection	9807306
32182855	32182878	17	-	27869803	PATU8988S	viability after 31 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.4803101638732235	[772]	[344,62,NaN]	-5	hSpCas9	negative selection	9889751
32183246	32183269	17	-	27869803	PATU8988S	viability after 31 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.5779368571263224	[907]	[198,297,NaN]	-6	hSpCas9	negative selection	9889752
32209365	32209388	17	+	27869803	PATU8988S	viability after 31 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	-0.29775594837060726	[1119]	[317,420,NaN]	-3	hSpCas9	negative selection	9889753
32211121	32211144	17	-	27869803	PATU8988S	viability after 31 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.8161750460508805	[344]	[133,379,NaN]	8	hSpCas9	negative selection	9889754
32253111	32253134	17	-	27869803	PATU8988S	viability after 31 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-0.19142795492111442	[199]	[64,75,NaN]	-2	hSpCas9	negative selection	9889755
32182855	32182878	17	-	27869803	HPAFII	viability after 35 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.08399165907375766	[330]	[107,255,NaN]	0	hSpCas9	negative selection	9972200
32183246	32183269	17	-	27869803	HPAFII	viability after 35 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.11117715774883141	[376]	[163,190,NaN]	0	hSpCas9	negative selection	9972201
32209365	32209388	17	+	27869803	HPAFII	viability after 35 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	1.0919462737839458	[448]	[357,626,NaN]	7	hSpCas9	negative selection	9972202
32211121	32211144	17	-	27869803	HPAFII	viability after 35 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	1.2836783579658793	[208]	[28,514,NaN]	8	hSpCas9	negative selection	9972203
32253111	32253134	17	-	27869803	HPAFII	viability after 35 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	-1.4300699869605102	[61]	[13,8,NaN]	-6	hSpCas9	negative selection	9972204
32182855	32182878	17	-	27869803	HPAFII	viability after 31 days	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.7319106788897456	[330]	[241,380,NaN]	5	hSpCas9	negative selection	10054649
32183246	32183269	17	-	27869803	HPAFII	viability after 31 days	TTCTCCTCTGGGCAGTACAGGGG	RHOT1	ENSG00000126858	-0.19194230780494448	[376]	[178,191,NaN]	-1	hSpCas9	negative selection	10054650
32209365	32209388	17	+	27869803	HPAFII	viability after 31 days	AGACAGACTCTCCCGAGACATGG	RHOT1	ENSG00000126858	0.6072520944724443	[448]	[324,447,NaN]	4	hSpCas9	negative selection	10054651
32211121	32211144	17	-	27869803	HPAFII	viability after 31 days	GGTGCACATACAGCGTAACCGGG	RHOT1	ENSG00000126858	0.6605650825950814	[208]	[26,357,NaN]	5	hSpCas9	negative selection	10054652
32253111	32253134	17	-	27869803	HPAFII	viability after 31 days	TTAGTCTCAGTGCAGGCGCCAGG	RHOT1	ENSG00000126858	0.10854307323630286	[61]	[48,24,NaN]	0	hSpCas9	negative selection	10054653
32183242	32183265	17	+	28162770	P31FUJ	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.1566711351984078	[202]	[213]	-1	hSpCas9	negative selection	10225157
32192207	32192230	17	-	28162770	P31FUJ	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.19754835035896942	[163]	[220]	2	hSpCas9	negative selection	10225158
32199510	32199533	17	+	28162770	P31FUJ	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.4421704620393726	[232]	[371]	5	hSpCas9	negative selection	10225159
32182844	32182867	17	+	28162770	P31FUJ	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	0.220915763028525	[45]	[62]	2	hSpCas9	negative selection	10225160
32198957	32198980	17	-	28162770	P31FUJ	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.5894644270471535	[219]	[388]	6	hSpCas9	negative selection	10225161
32208224	32208247	17	-	28162770	P31FUJ	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	1.091432357856153	[124]	[312]	9	hSpCas9	negative selection	10225162
32182775	32182798	17	+	28162770	P31FUJ	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	-0.06479440596635694	[250]	[281]	0	hSpCas9	negative selection	10225163
32193208	32193231	17	+	28162770	P31FUJ	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	-0.0038964761192562675	[220]	[258]	0	hSpCas9	negative selection	10225164
32199526	32199549	17	+	28162770	P31FUJ	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	0.06501343435617601	[361]	[444]	0	hSpCas9	negative selection	10225165
32182855	32182878	17	-	28162770	P31FUJ	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.2818264364190348	[867]	[838]	-3	hSpCas9	negative selection	10225166
32183242	32183265	17	+	28162770	TF1	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	NaN	[NaN]	[170]	1	hSpCas9	negative selection	10394523
32192207	32192230	17	-	28162770	TF1	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	NaN	[NaN]	[119]	1	hSpCas9	negative selection	10394524
32199510	32199533	17	+	28162770	TF1	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	NaN	[NaN]	[463]	1	hSpCas9	negative selection	10394525
32182844	32182867	17	+	28162770	TF1	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	NaN	[NaN]	[52]	1	hSpCas9	negative selection	10394526
32198957	32198980	17	-	28162770	TF1	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	NaN	[NaN]	[75]	1	hSpCas9	negative selection	10394527
32208224	32208247	17	-	28162770	TF1	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	NaN	[NaN]	[182]	1	hSpCas9	negative selection	10394528
32182775	32182798	17	+	28162770	TF1	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	NaN	[NaN]	[146]	1	hSpCas9	negative selection	10394529
32193208	32193231	17	+	28162770	TF1	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	NaN	[NaN]	[87]	1	hSpCas9	negative selection	10394530
32199526	32199549	17	+	28162770	TF1	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	NaN	[NaN]	[148]	1	hSpCas9	negative selection	10394531
32182855	32182878	17	-	28162770	TF1	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	NaN	[NaN]	[623]	1	hSpCas9	negative selection	10394532
32183242	32183265	17	+	28162770	SKM1	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.21752666616463184	[71]	[147]	2	hSpCas9	negative selection	10563889
32192207	32192230	17	-	28162770	SKM1	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.5809935985182104	[21]	[25]	-4	hSpCas9	negative selection	10563890
32199510	32199533	17	+	28162770	SKM1	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.3706433799203904	[97]	[223]	3	hSpCas9	negative selection	10563891
32182844	32182867	17	+	28162770	SKM1	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	-1.0025739436638264	[16]	[14]	-6	hSpCas9	negative selection	10563892
32198957	32198980	17	-	28162770	SKM1	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.700225047351188	[46]	[134]	6	hSpCas9	negative selection	10563893
32208224	32208247	17	-	28162770	SKM1	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.5779289088666298	[35]	[94]	5	hSpCas9	negative selection	10563894
32182775	32182798	17	+	28162770	SKM1	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.6109577092541005	[49]	[134]	6	hSpCas9	negative selection	10563895
32193208	32193231	17	+	28162770	SKM1	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.28605804783544736	[44]	[96]	2	hSpCas9	negative selection	10563896
32199526	32199549	17	+	28162770	SKM1	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.28284288691397397	[63]	[92]	-2	hSpCas9	negative selection	10563897
32182855	32182878	17	-	28162770	SKM1	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.34151984584177764	[166]	[232]	-3	hSpCas9	negative selection	10563898
32183242	32183265	17	+	28162770	SKM1	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.21752666616463184	[71]	[147]	2	hSpCas9	negative selection	10733255
32192207	32192230	17	-	28162770	SKM1	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.5809935985182104	[21]	[25]	-4	hSpCas9	negative selection	10733256
32199510	32199533	17	+	28162770	SKM1	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.3706433799203904	[97]	[223]	3	hSpCas9	negative selection	10733257
32182844	32182867	17	+	28162770	SKM1	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	-1.0025739436638264	[16]	[14]	-6	hSpCas9	negative selection	10733258
32198957	32198980	17	-	28162770	SKM1	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.700225047351188	[46]	[134]	6	hSpCas9	negative selection	10733259
32208224	32208247	17	-	28162770	SKM1	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.5779289088666298	[35]	[94]	5	hSpCas9	negative selection	10733260
32182775	32182798	17	+	28162770	SKM1	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.6109577092541005	[49]	[134]	6	hSpCas9	negative selection	10733261
32193208	32193231	17	+	28162770	SKM1	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.28605804783544736	[44]	[96]	2	hSpCas9	negative selection	10733262
32199526	32199549	17	+	28162770	SKM1	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.28284288691397397	[63]	[92]	-2	hSpCas9	negative selection	10733263
32182855	32182878	17	-	28162770	SKM1	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.34151984584177764	[166]	[232]	-3	hSpCas9	negative selection	10733264
32183242	32183265	17	+	28162770	PL21	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.035752796588437774	[259]	[115]	0	hSpCas9	negative selection	10902621
32192207	32192230	17	-	28162770	PL21	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.7010822310325063	[126]	[33]	-5	hSpCas9	negative selection	10902622
32199510	32199533	17	+	28162770	PL21	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	1.2496082907536197	[257]	[266]	8	hSpCas9	negative selection	10902623
32182844	32182867	17	+	28162770	PL21	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	0.8216279912355902	[51]	[39]	7	hSpCas9	negative selection	10902624
32198957	32198980	17	-	28162770	PL21	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	1.4436966458238105	[173]	[205]	9	hSpCas9	negative selection	10902625
32208224	32208247	17	-	28162770	PL21	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.5371746017668908	[170]	[107]	5	hSpCas9	negative selection	10902626
32182775	32182798	17	+	28162770	PL21	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.4559785189189099	[133]	[79]	4	hSpCas9	negative selection	10902627
32193208	32193231	17	+	28162770	PL21	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	-0.1624304648953881	[161]	[62]	-1	hSpCas9	negative selection	10902628
32199526	32199549	17	+	28162770	PL21	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.01709110173134898	[278]	[119]	0	hSpCas9	negative selection	10902629
32182855	32182878	17	-	28162770	PL21	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.2039267669685203	[523]	[197]	-1	hSpCas9	negative selection	10902630
32183242	32183265	17	+	28162770	P31FUJ	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.1566711351984078	[202]	[213]	-1	hSpCas9	negative selection	11071987
32192207	32192230	17	-	28162770	P31FUJ	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.19754835035896942	[163]	[220]	2	hSpCas9	negative selection	11071988
32199510	32199533	17	+	28162770	P31FUJ	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.4421704620393726	[232]	[371]	5	hSpCas9	negative selection	11071989
32182844	32182867	17	+	28162770	P31FUJ	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	0.220915763028525	[45]	[62]	2	hSpCas9	negative selection	11071990
32198957	32198980	17	-	28162770	P31FUJ	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.5894644270471535	[219]	[388]	6	hSpCas9	negative selection	11071991
32208224	32208247	17	-	28162770	P31FUJ	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	1.091432357856153	[124]	[312]	9	hSpCas9	negative selection	11071992
32182775	32182798	17	+	28162770	P31FUJ	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	-0.06479440596635694	[250]	[281]	0	hSpCas9	negative selection	11071993
32193208	32193231	17	+	28162770	P31FUJ	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	-0.0038964761192562675	[220]	[258]	0	hSpCas9	negative selection	11071994
32199526	32199549	17	+	28162770	P31FUJ	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	0.06501343435617601	[361]	[444]	0	hSpCas9	negative selection	11071995
32182855	32182878	17	-	28162770	P31FUJ	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.2818264364190348	[867]	[838]	-3	hSpCas9	negative selection	11071996
32183242	32183265	17	+	28162770	OCIAML5	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.053700432033647605	[91]	[159]	0	hSpCas9	negative selection	11241353
32192207	32192230	17	-	28162770	OCIAML5	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.23607717452221638	[91]	[140]	-2	hSpCas9	negative selection	11241354
32199510	32199533	17	+	28162770	OCIAML5	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.40846097935922204	[143]	[344]	3	hSpCas9	negative selection	11241355
32182844	32182867	17	+	28162770	OCIAML5	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	-1.328504614806984	[31]	[22]	-6	hSpCas9	negative selection	11241356
32198957	32198980	17	-	28162770	OCIAML5	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.13313642720792257	[97]	[193]	1	hSpCas9	negative selection	11241357
32208224	32208247	17	-	28162770	OCIAML5	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.44255417802763	[127]	[313]	4	hSpCas9	negative selection	11241358
32182775	32182798	17	+	28162770	OCIAML5	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.19373711874912775	[123]	[255]	1	hSpCas9	negative selection	11241359
32193208	32193231	17	+	28162770	OCIAML5	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.7073608377500218	[94]	[279]	6	hSpCas9	negative selection	11241360
32199526	32199549	17	+	28162770	OCIAML5	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	0.6764826209518929	[112]	[325]	6	hSpCas9	negative selection	11241361
32182855	32182878	17	-	28162770	OCIAML5	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.5497023272593353	[354]	[937]	5	hSpCas9	negative selection	11241362
32183242	32183265	17	+	28162770	EOL1	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.4375084360221373	[400]	[155]	-3	hSpCas9	negative selection	11410719
32192207	32192230	17	-	28162770	EOL1	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.9718534863488915	[119]	[123]	8	hSpCas9	negative selection	11410720
32199510	32199533	17	+	28162770	EOL1	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.26676448642451067	[578]	[366]	2	hSpCas9	negative selection	11410721
32182844	32182867	17	+	28162770	EOL1	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	1.252352432794566	[97]	[122]	9	hSpCas9	negative selection	11410722
32198957	32198980	17	-	28162770	EOL1	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	1.0680918974777691	[324]	[358]	8	hSpCas9	negative selection	11410723
32208224	32208247	17	-	28162770	EOL1	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.5581841190273802	[271]	[210]	5	hSpCas9	negative selection	11410724
32182775	32182798	17	+	28162770	EOL1	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.7498692401575875	[227]	[201]	6	hSpCas9	negative selection	11410725
32193208	32193231	17	+	28162770	EOL1	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	-0.41552767002708724	[199]	[78]	-3	hSpCas9	negative selection	11410726
32199526	32199549	17	+	28162770	EOL1	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.5070639522438251	[320]	[118]	-4	hSpCas9	negative selection	11410727
32182855	32182878	17	-	28162770	EOL1	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.6161380008578663	[716]	[578]	5	hSpCas9	negative selection	11410728
32183242	32183265	17	+	28162770	MOLM13	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.8464010047983087	[289]	[376]	6	hSpCas9	negative selection	11580085
32192207	32192230	17	-	28162770	MOLM13	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.1235548736089368	[145]	[114]	0	hSpCas9	negative selection	11580086
32199510	32199533	17	+	28162770	MOLM13	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.5642154986196032	[419]	[448]	4	hSpCas9	negative selection	11580087
32182844	32182867	17	+	28162770	MOLM13	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	1.2693437021624987	[69]	[121]	8	hSpCas9	negative selection	11580088
32198957	32198980	17	-	28162770	MOLM13	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.17218028256521006	[258]	[210]	1	hSpCas9	negative selection	11580089
32208224	32208247	17	-	28162770	MOLM13	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.24958391773137403	[234]	[201]	1	hSpCas9	negative selection	11580090
32182775	32182798	17	+	28162770	MOLM13	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.25949023228763085	[170]	[147]	1	hSpCas9	negative selection	11580091
32193208	32193231	17	+	28162770	MOLM13	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.20765360906345848	[205]	[171]	1	hSpCas9	negative selection	11580092
32199526	32199549	17	+	28162770	MOLM13	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.4538591727910529	[304]	[160]	-3	hSpCas9	negative selection	11580093
32182855	32182878	17	-	28162770	MOLM13	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.4359478900863858	[884]	[472]	-3	hSpCas9	negative selection	11580094
32183242	32183265	17	+	28162770	HEL	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.4068816872366623	[304]	[258]	3	hSpCas9	negative selection	11749451
32192207	32192230	17	-	28162770	HEL	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.7932985085757382	[99]	[110]	7	hSpCas9	negative selection	11749452
32199510	32199533	17	+	28162770	HEL	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.7137053533545005	[356]	[374]	6	hSpCas9	negative selection	11749453
32182844	32182867	17	+	28162770	HEL	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	0.9005365894680037	[45]	[54]	7	hSpCas9	negative selection	11749454
32198957	32198980	17	-	28162770	HEL	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.06032301338954718	[282]	[188]	0	hSpCas9	negative selection	11749455
32208224	32208247	17	-	28162770	HEL	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.8286892573687448	[188]	[214]	7	hSpCas9	negative selection	11749456
32182775	32182798	17	+	28162770	HEL	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.9739447404757302	[185]	[233]	8	hSpCas9	negative selection	11749457
32193208	32193231	17	+	28162770	HEL	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.7890572204903354	[177]	[196]	6	hSpCas9	negative selection	11749458
32199526	32199549	17	+	28162770	HEL	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.8004955591091389	[309]	[113]	-5	hSpCas9	negative selection	11749459
32182855	32182878	17	-	28162770	HEL	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	-0.30603584464447	[663]	[343]	-2	hSpCas9	negative selection	11749460
32183242	32183265	17	+	28162770	MV411	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.15485681796587814	[262]	[198]	-1	hSpCas9	negative selection	11918817
32192207	32192230	17	-	28162770	MV411	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	0.2474375507827754	[95]	[95]	2	hSpCas9	negative selection	11918818
32199510	32199533	17	+	28162770	MV411	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	0.6302112008906646	[259]	[338]	6	hSpCas9	negative selection	11918819
32182844	32182867	17	+	28162770	MV411	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	-1.7525624492172247	[47]	[11]	-8	hSpCas9	negative selection	11918820
32198957	32198980	17	-	28162770	MV411	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.28632656417637026	[182]	[187]	2	hSpCas9	negative selection	11918821
32208224	32208247	17	-	28162770	MV411	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	1.0213694713029289	[130]	[223]	8	hSpCas9	negative selection	11918822
32182775	32182798	17	+	28162770	MV411	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.9751846149196028	[156]	[259]	8	hSpCas9	negative selection	11918823
32193208	32193231	17	+	28162770	MV411	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.6818403749285502	[147]	[199]	6	hSpCas9	negative selection	11918824
32199526	32199549	17	+	28162770	MV411	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.4195791658208664	[207]	[130]	-3	hSpCas9	negative selection	11918825
32182855	32182878	17	-	28162770	MV411	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.5848619819933059	[462]	[584]	5	hSpCas9	negative selection	11918826
32183242	32183265	17	+	28162770	MONOMAC1	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	0.33474561580242757	[174]	[252]	3	hSpCas9	negative selection	12088183
32192207	32192230	17	-	28162770	MONOMAC1	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	-0.7913980457829592	[76]	[50]	-6	hSpCas9	negative selection	12088184
32199510	32199533	17	+	28162770	MONOMAC1	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	-0.1029933571444156	[192]	[205]	-1	hSpCas9	negative selection	12088185
32182844	32182867	17	+	28162770	MONOMAC1	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	1.3054634934696292	[29]	[84]	9	hSpCas9	negative selection	12088186
32198957	32198980	17	-	28162770	MONOMAC1	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	-0.45367348169519695	[134]	[112]	-4	hSpCas9	negative selection	12088187
32208224	32208247	17	-	28162770	MONOMAC1	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	1.0112803890651714	[102]	[237]	8	hSpCas9	negative selection	12088188
32182775	32182798	17	+	28162770	MONOMAC1	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	-0.03161323785218201	[106]	[119]	0	hSpCas9	negative selection	12088189
32193208	32193231	17	+	28162770	MONOMAC1	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.07242382793367323	[111]	[134]	0	hSpCas9	negative selection	12088190
32199526	32199549	17	+	28162770	MONOMAC1	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	0.552155049439912	[140]	[236]	5	hSpCas9	negative selection	12088191
32182855	32182878	17	-	28162770	MONOMAC1	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.42299842057522863	[297]	[457]	4	hSpCas9	negative selection	12088192
32183242	32183265	17	+	28162770	OCIAML2	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.263304851994655	[54]	[253]	-2	hSpCas9	negative selection	12257549
32192207	32192230	17	-	28162770	OCIAML2	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	1.1440800188730469	[11]	[146]	9	hSpCas9	negative selection	12257550
32199510	32199533	17	+	28162770	OCIAML2	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	-0.38697189619525174	[66]	[283]	-3	hSpCas9	negative selection	12257551
32182844	32182867	17	+	28162770	OCIAML2	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	-0.14870173035479883	[11]	[59]	-1	hSpCas9	negative selection	12257552
32198957	32198980	17	-	28162770	OCIAML2	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.6918200556380045	[42]	[384]	6	hSpCas9	negative selection	12257553
32208224	32208247	17	-	28162770	OCIAML2	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	1.0851863298194784	[24]	[293]	9	hSpCas9	negative selection	12257554
32182775	32182798	17	+	28162770	OCIAML2	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.6378946315360078	[23]	[206]	6	hSpCas9	negative selection	12257555
32193208	32193231	17	+	28162770	OCIAML2	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	0.22124787939550689	[25]	[167]	2	hSpCas9	negative selection	12257556
32199526	32199549	17	+	28162770	OCIAML2	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	0.841648099611482	[29]	[297]	7	hSpCas9	negative selection	12257557
32182855	32182878	17	-	28162770	OCIAML2	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.04062298929598551	[86]	[495]	0	hSpCas9	negative selection	12257558
32183242	32183265	17	+	28162770	OCIAML3	viability	AGGGCCCCTGTACTGCCCAGAGG	RHOT1	ENSG00000126858	-0.19947579647996072	[61]	[162]	-1	hSpCas9	negative selection	12426915
32192207	32192230	17	-	28162770	OCIAML3	viability	GTAAGGGCTTTTATACAAGCTGG	RHOT1	ENSG00000126858	1.5818421950752546	[26]	[243]	9	hSpCas9	negative selection	12426916
32199510	32199533	17	+	28162770	OCIAML3	viability	AGAGGCTGGATAACCTACCAGGG	RHOT1	ENSG00000126858	-0.12834406797535147	[83]	[231]	-1	hSpCas9	negative selection	12426917
32182844	32182867	17	+	28162770	OCIAML3	viability	AGAAATAGAAACCTGTGTGGAGG	RHOT1	ENSG00000126858	0.2684888359259019	[10]	[39]	2	hSpCas9	negative selection	12426918
32198957	32198980	17	-	28162770	OCIAML3	viability	AATTCAGTAGTGCAATCAGGAGG	RHOT1	ENSG00000126858	0.42267110082246706	[42]	[173]	4	hSpCas9	negative selection	12426919
32208224	32208247	17	-	28162770	OCIAML3	viability	CAAGTGAAGGCTTGTGGTGGAGG	RHOT1	ENSG00000126858	0.8971328380404723	[44]	[252]	7	hSpCas9	negative selection	12426920
32182775	32182798	17	+	28162770	OCIAML3	viability	TGGGAACAAATCTGATCTGGTGG	RHOT1	ENSG00000126858	0.2578937206867029	[40]	[147]	2	hSpCas9	negative selection	12426921
32193208	32193231	17	+	28162770	OCIAML3	viability	AGTGATGGTGTGGCTGACAGTGG	RHOT1	ENSG00000126858	-0.5636339912806445	[46]	[95]	-4	hSpCas9	negative selection	12426922
32199526	32199549	17	+	28162770	OCIAML3	viability	ACCAGGGATTCCTTTCCCAGTGG	RHOT1	ENSG00000126858	-0.7739863064303985	[80]	[142]	-5	hSpCas9	negative selection	12426923
32182855	32182878	17	-	28162770	OCIAML3	viability	TACAAGGCATACCTCCACACAGG	RHOT1	ENSG00000126858	0.6638764001518458	[151]	[726]	6	hSpCas9	negative selection	12426924