Gene2Function

Crispr

Gene Info

Species: Human (Homo sapiens)
GeneID: 135154
Symbol: SDHAF4
Description: succinate dehydrogenase complex assembly factor 4

DataSource: http://genomecrispr.dkfz.de/#!/results/SDHAF4

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
70573505	70573528	6	-	25494202	A375	resistance to PLX-4720 (puromycin)	ATTTGGGAGGCTGAGGCAGGTGG	SDHAF4	ENSG00000154079	1.0311422578945828	[46,428]	[158,53]	4	dCas9-VP64	positive selection	2400171
70573505	70573528	6	-	25494202	A375	resistance to PLX-4720 (zeocin)	ATTTGGGAGGCTGAGGCAGGTGG	SDHAF4	ENSG00000154079	0.6350691816100226	[174,241]	[44,161]	5	dCas9-VP64	positive selection	2495426
70566984	70567007	6	-	26780180	HT29	viability (Avana library 4 designs)	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	1.51263717569919	[]	[]	6	hSpCas9	negative selection	3457592
70579420	70579443	6	-	26780180	HT29	viability (Avana library 4 designs)	CCTCAGAGAATGACACAGAAGGG	SDHAF4	ENSG00000154079	-0.746040765148924	[]	[]	-8	hSpCas9	negative selection	3457593
70566959	70566982	6	-	26780180	HT29	viability (Avana library 4 designs)	GAGACCCAGCTAAGCAACCAGGG	SDHAF4	ENSG00000154079	1.3266638193082	[]	[]	5	hSpCas9	negative selection	3457594
70566972	70566995	6	+	26780180	HT29	viability (Avana library 4 designs)	GCTGGGTCTCGGCCACGGCGTGG	SDHAF4	ENSG00000154079	0.828154148361771	[]	[]	3	hSpCas9	negative selection	3457595
70566984	70567007	6	-	26780180	A375	viability (Avana lentiCRISPRv2)	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	-7.37209786764419	[]	[]	-6	hSpCas9	negative selection	3556616
70579420	70579443	6	-	26780180	A375	viability (Avana lentiCRISPRv2)	CCTCAGAGAATGACACAGAAGGG	SDHAF4	ENSG00000154079	-8.45171624769734	[]	[]	-7	hSpCas9	negative selection	3556617
70566959	70566982	6	-	26780180	A375	viability (Avana lentiCRISPRv2)	GAGACCCAGCTAAGCAACCAGGG	SDHAF4	ENSG00000154079	-3.61632937782623	[]	[]	-4	hSpCas9	negative selection	3556618
70566972	70566995	6	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCTGGGTCTCGGCCACGGCGTGG	SDHAF4	ENSG00000154079	-2.68488356271518	[]	[]	-3	hSpCas9	negative selection	3556619
70579494	70579517	6	+	26780180	A375	viability (Avana lentiCRISPRv2)	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	-5.69408782416601	[]	[]	-6	hSpCas9	negative selection	3556620
70579489	70579512	6	-	26780180	A375	viability (Avana lentiCRISPRv2)	TGGTAACTTCGGCTTCTTAAGGG	SDHAF4	ENSG00000154079	-2.62803452403031	[]	[]	-3	hSpCas9	negative selection	3556621
70566984	70567007	6	-	26780180	A375	viability (Avana lentiGuide)	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	16.0630215079174	[]	[]	8	hSpCas9	negative selection	3665275
70579420	70579443	6	-	26780180	A375	viability (Avana lentiGuide)	CCTCAGAGAATGACACAGAAGGG	SDHAF4	ENSG00000154079	5.85166129969654	[]	[]	5	hSpCas9	negative selection	3665276
70566959	70566982	6	-	26780180	A375	viability (Avana lentiGuide)	GAGACCCAGCTAAGCAACCAGGG	SDHAF4	ENSG00000154079	9.95527952453581	[]	[]	7	hSpCas9	negative selection	3665277
70566972	70566995	6	+	26780180	A375	viability (Avana lentiGuide)	GCTGGGTCTCGGCCACGGCGTGG	SDHAF4	ENSG00000154079	26.5464639622239	[]	[]	9	hSpCas9	negative selection	3665278
70579494	70579517	6	+	26780180	A375	viability (Avana lentiGuide)	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	16.4295194475366	[]	[]	8	hSpCas9	negative selection	3665279
70579489	70579512	6	-	26780180	A375	viability (Avana lentiGuide)	TGGTAACTTCGGCTTCTTAAGGG	SDHAF4	ENSG00000154079	-2.79082223531702	[]	[]	-5	hSpCas9	negative selection	3665280
70566984	70567007	6	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	-0.16438542465611117	[4]	[3,3]	-3	hSpCas9	positive selection	3773882
70579420	70579443	6	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CCTCAGAGAATGACACAGAAGGG	SDHAF4	ENSG00000154079	-0.502016739536881	[4]	[3,1]	-6	hSpCas9	positive selection	3773883
70566959	70566982	6	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GAGACCCAGCTAAGCAACCAGGG	SDHAF4	ENSG00000154079	-0.5371715710459669	[4]	[3,2]	-7	hSpCas9	positive selection	3773884
70566972	70566995	6	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCTGGGTCTCGGCCACGGCGTGG	SDHAF4	ENSG00000154079	0.21468982369431405	[5]	[5,6]	6	hSpCas9	positive selection	3773885
70579494	70579517	6	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.15824660461909634	[5]	[5,5]	4	hSpCas9	positive selection	3773886
70579489	70579512	6	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGGTAACTTCGGCTTCTTAAGGG	SDHAF4	ENSG00000154079	-0.07960489057361725	[7]	[6,6]	-1	hSpCas9	positive selection	3773887
70566984	70567007	6	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	-0.008794527988752078	[5]	[5,3]	0	hSpCas9	positive selection	3882489
70579420	70579443	6	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CCTCAGAGAATGACACAGAAGGG	SDHAF4	ENSG00000154079	-0.2694675293897463	[4]	[2,3]	-4	hSpCas9	positive selection	3882490
70566959	70566982	6	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GAGACCCAGCTAAGCAACCAGGG	SDHAF4	ENSG00000154079	-0.10236265858269562	[5]	[4,3]	-1	hSpCas9	positive selection	3882491
70566972	70566995	6	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCTGGGTCTCGGCCACGGCGTGG	SDHAF4	ENSG00000154079	-0.17327791964880818	[5]	[4,4]	-2	hSpCas9	positive selection	3882492
70579494	70579517	6	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.09818650885747128	[5]	[3,5]	2	hSpCas9	positive selection	3882493
70579489	70579512	6	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGGTAACTTCGGCTTCTTAAGGG	SDHAF4	ENSG00000154079	0.3323508821071304	[6]	[7,7]	8	hSpCas9	positive selection	3882494
70579541	70579564	6	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CCAGTGGTTCTTTCTCTAAATGG	SDHAF4	ENSG00000154079	-0.19838801831988384	[3]	[1,1]	-5	hSpCas9	positive selection	4187712
70579509	70579532	6	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	-0.22737798653062602	[2]	[1,1]	-5	hSpCas9	positive selection	4187715
70566933	70566956	6	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	-0.09032385806571278	[4]	[2,2]	-2	hSpCas9	positive selection	4187716
70579541	70579564	6	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CCAGTGGTTCTTTCTCTAAATGG	SDHAF4	ENSG00000154079	-0.3541453439659285	[3]	[0,3,0,0]	-5	hSpCas9	positive selection	4251734
70579509	70579532	6	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.9744917675720411	[3]	[0,2,0,3]	6	hSpCas9	positive selection	4251737
70566933	70566956	6	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	1.0487790270778528	[3]	[4,2,2,3]	7	hSpCas9	positive selection	4251738
70579509	70579532	6	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	-0.10530302665776331	[1]	[2,0]	-2	hSpCas9	positive selection	4260198
70566933	70566956	6	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.1603250280260324	[3]	[4,1]	3	hSpCas9	positive selection	4260199
70588665	70588688	6	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.032638982694556076	[2]	[2,1]	0	hSpCas9	positive selection	4260200
70566984	70567007	6	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.17213701818225977	[3]	[3,2]	3	hSpCas9	positive selection	4324479
70588679	70588702	6	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.5124404659470831	[3]	[4,4]	9	hSpCas9	positive selection	4324480
70579494	70579517	6	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.042563555513224716	[2]	[3,0]	0	hSpCas9	positive selection	4324481
70579509	70579532	6	-	27260156	BXPC3	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.5105753879527366	[172]	[263,251,258,252]	9	hSpCas9	negative selection	5532658
70566933	70566956	6	+	27260156	BXPC3	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3191051701744554	[724]	[730,744,1148,1224]	7	hSpCas9	negative selection	5532659
70588665	70588688	6	+	27260156	BXPC3	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.004758398393657326	[388]	[409,491,298,422]	0	hSpCas9	negative selection	5532660
70566984	70567007	6	-	27260156	BXPC3	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.383680094019605	[545]	[680,770,766,762]	7	hSpCas9	negative selection	5596939
70588679	70588702	6	-	27260156	BXPC3	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.1952550632376262	[956]	[1167,1020,1065,1347]	4	hSpCas9	negative selection	5596940
70579494	70579517	6	+	27260156	BXPC3	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.5095478218725525	[221]	[261,216,356,521]	9	hSpCas9	negative selection	5596941
70579509	70579532	6	-	27260156	A673	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.7342087017850524	[172]	[392,318,239,441]	9	hSpCas9	negative selection	5653909
70566933	70566956	6	+	27260156	A673	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3776705279047581	[724]	[990,1114,1221,1179]	8	hSpCas9	negative selection	5653910
70588665	70588688	6	+	27260156	A673	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.1948385187067575	[388]	[725,365,418,660]	4	hSpCas9	negative selection	5653911
70566984	70567007	6	-	27260156	A673	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.3802970739247551	[545]	[961,837,790,832]	8	hSpCas9	negative selection	5718190
70588679	70588702	6	-	27260156	A673	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.19444197854273937	[956]	[1544,1172,1221,1349]	4	hSpCas9	negative selection	5718191
70579494	70579517	6	+	27260156	A673	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.07684231395821994	[221]	[274,233,363,248]	1	hSpCas9	negative selection	5718192
70579509	70579532	6	-	27260156	A375	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.5045713860756468	[172]	[418,252,193,276]	7	hSpCas9	negative selection	5775160
70566933	70566956	6	+	27260156	A375	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	-0.440736470774757	[724]	[511,876,574,569]	-6	hSpCas9	negative selection	5775161
70588665	70588688	6	+	27260156	A375	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.32909520133797976	[388]	[389,333,312,399]	-5	hSpCas9	negative selection	5775162
70566984	70567007	6	-	27260156	A375	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	-0.12957574091092522	[545]	[785,641,575,361]	-2	hSpCas9	negative selection	5839441
70588679	70588702	6	-	27260156	A375	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.39694813345555335	[956]	[1619,1369,1338,1531]	6	hSpCas9	negative selection	5839442
70579494	70579517	6	+	27260156	A375	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.2491610857512917	[221]	[224,274,276,433]	3	hSpCas9	negative selection	5839443
70579509	70579532	6	-	27260156	COLO741	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.6333822308113236	[172]	[327,304,389]	9	hSpCas9	negative selection	5896411
70566933	70566956	6	+	27260156	COLO741	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.45360864853476945	[724]	[1235,1445,1128]	8	hSpCas9	negative selection	5896412
70588665	70588688	6	+	27260156	COLO741	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.40990497631153755	[388]	[728,689,572]	8	hSpCas9	negative selection	5896413
70566984	70567007	6	-	27260156	COLO741	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.2651658375467812	[545]	[923,919,687]	5	hSpCas9	negative selection	5960692
70588679	70588702	6	-	27260156	COLO741	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.3258109195909047	[956]	[2000,1530,1131]	6	hSpCas9	negative selection	5960693
70579494	70579517	6	+	27260156	COLO741	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.6541401620910634	[221]	[439,428,466]	9	hSpCas9	negative selection	5960694
70579509	70579532	6	-	27260156	CAL120	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.7630927588967826	[172]	[304,313,465]	9	hSpCas9	negative selection	6017662
70566933	70566956	6	+	27260156	CAL120	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3521784901665608	[724]	[1012,1406,1015]	5	hSpCas9	negative selection	6017663
70588665	70588688	6	+	27260156	CAL120	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.47553916864808127	[388]	[1017,557,497]	7	hSpCas9	negative selection	6017664
70566984	70567007	6	-	27260156	CAL120	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.462356415521616	[545]	[1144,791,902]	7	hSpCas9	negative selection	6081943
70588679	70588702	6	-	27260156	CAL120	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.4797544131253271	[956]	[1660,1803,1518]	7	hSpCas9	negative selection	6081944
70579494	70579517	6	+	27260156	CAL120	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.8987999637752773	[221]	[663,531,373]	9	hSpCas9	negative selection	6081945
70579509	70579532	6	-	27260156	CADOES1	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.5332725923776073	[172]	[119,294,355,157]	8	hSpCas9	negative selection	6138913
70566933	70566956	6	+	27260156	CADOES1	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.06372510697157385	[724]	[681,623,854,655]	1	hSpCas9	negative selection	6138914
70588665	70588688	6	+	27260156	CADOES1	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.3371730015224246	[388]	[261,382,235,263]	-5	hSpCas9	negative selection	6138915
70566984	70567007	6	-	27260156	CADOES1	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.2815045163819772	[545]	[712,579,598,574]	5	hSpCas9	negative selection	6203194
70588679	70588702	6	-	27260156	CADOES1	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.31176880853048305	[956]	[1210,1106,966,1134]	5	hSpCas9	negative selection	6203195
70579494	70579517	6	+	27260156	CADOES1	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.4314553590196609	[221]	[245,358,180,329]	7	hSpCas9	negative selection	6203196
70579509	70579532	6	-	27260156	EWS502	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.9056630817455316	[172]	[359,338,402,262]	9	hSpCas9	negative selection	6260164
70566933	70566956	6	+	27260156	EWS502	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.21845137901874612	[724]	[842,892,966,872]	4	hSpCas9	negative selection	6260165
70588665	70588688	6	+	27260156	EWS502	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.21314662558793995	[388]	[254,386,311,485]	-4	hSpCas9	negative selection	6260166
70566984	70567007	6	-	27260156	EWS502	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.22566671627301255	[545]	[631,703,690,679]	4	hSpCas9	negative selection	6324445
70588679	70588702	6	-	27260156	EWS502	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.5649983926734541	[956]	[1379,1288,1616,1748]	8	hSpCas9	negative selection	6324446
70579494	70579517	6	+	27260156	EWS502	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.5010745056573599	[221]	[341,371,365,240]	8	hSpCas9	negative selection	6324447
70579509	70579532	6	-	27260156	EW8	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.4885502968525137	[172]	[217,289,144,144]	8	hSpCas9	negative selection	6381415
70566933	70566956	6	+	27260156	EW8	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.18413884958049875	[724]	[732,858,505,605]	3	hSpCas9	negative selection	6381416
70588665	70588688	6	+	27260156	EW8	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.1494457489384688	[388]	[303,280,261,294]	-3	hSpCas9	negative selection	6381417
70566984	70567007	6	-	27260156	EW8	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.24563865541410101	[545]	[489,527,554,514]	4	hSpCas9	negative selection	6445696
70588679	70588702	6	-	27260156	EW8	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.17439453932437843	[956]	[915,922,696,996]	3	hSpCas9	negative selection	6445697
70579494	70579517	6	+	27260156	EW8	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	-0.023376566017395017	[221]	[169,162,168,205]	0	hSpCas9	negative selection	6445698
70579509	70579532	6	-	27260156	CORL105	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.5353010614569802	[172]	[363,221,294,296]	8	hSpCas9	negative selection	6502666
70566933	70566956	6	+	27260156	CORL105	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3082519610880782	[724]	[1167,847,1219,1001]	6	hSpCas9	negative selection	6502667
70588665	70588688	6	+	27260156	CORL105	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.04860434402651864	[388]	[369,411,528,461]	-1	hSpCas9	negative selection	6502668
70566984	70567007	6	-	27260156	CORL105	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.46416055851016935	[545]	[696,1056,1031,754]	8	hSpCas9	negative selection	6566947
70588679	70588702	6	-	27260156	CORL105	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.19231888034109357	[956]	[1130,1178,1493,1345]	3	hSpCas9	negative selection	6566948
70579494	70579517	6	+	27260156	CORL105	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.27951871485736457	[221]	[256,233,410,373]	5	hSpCas9	negative selection	6566949
70579509	70579532	6	-	27260156	HS294T	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	1.383013520517107	[172]	[149,238,145,159]	9	hSpCas9	negative selection	6623917
70566933	70566956	6	+	27260156	HS294T	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.020933192462036065	[724]	[264,275,282,320]	0	hSpCas9	negative selection	6623918
70588665	70588688	6	+	27260156	HS294T	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.31160137431206403	[388]	[112,114,112,148]	-4	hSpCas9	negative selection	6623919
70566984	70567007	6	-	27260156	HS294T	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.03226763239502323	[545]	[210,224,180,251]	0	hSpCas9	negative selection	6688198
70588679	70588702	6	-	27260156	HS294T	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.4951103992540091	[956]	[445,524,541,589]	6	hSpCas9	negative selection	6688199
70579494	70579517	6	+	27260156	HS294T	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.006177459722637391	[221]	[92,61,66,129]	0	hSpCas9	negative selection	6688200
70579509	70579532	6	-	27260156	HCC44	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.3265858449912653	[172]	[252,204,281,251]	5	hSpCas9	negative selection	6745168
70566933	70566956	6	+	27260156	HCC44	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3250967735427307	[724]	[994,1072,1117,923]	5	hSpCas9	negative selection	6745169
70588665	70588688	6	+	27260156	HCC44	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.14882493040559508	[388]	[498,434,697,363]	2	hSpCas9	negative selection	6745170
70566984	70567007	6	-	27260156	HCC44	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.19515225551641707	[545]	[654,465,719,1002]	3	hSpCas9	negative selection	6809449
70588679	70588702	6	-	27260156	HCC44	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.5381875464217849	[956]	[1340,1435,1747,1770]	8	hSpCas9	negative selection	6809450
70579494	70579517	6	+	27260156	HCC44	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.2887112519714117	[221]	[358,305,342,229]	5	hSpCas9	negative selection	6809451
70579509	70579532	6	-	27260156	G402	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	1.3576999137470112	[172]	[775,182,611,600]	9	hSpCas9	negative selection	6866419
70566933	70566956	6	+	27260156	G402	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	-0.20799645401626415	[724]	[835,749,956,537]	-3	hSpCas9	negative selection	6866420
70588665	70588688	6	+	27260156	G402	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.0011107716704961135	[388]	[263,409,926,272]	0	hSpCas9	negative selection	6866421
70566984	70567007	6	-	27260156	G402	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.4008276943467076	[545]	[875,674,847,1092]	5	hSpCas9	negative selection	6930700
70588679	70588702	6	-	27260156	G402	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.46489039175722113	[956]	[1704,2253,1017,1518]	6	hSpCas9	negative selection	6930701
70579494	70579517	6	+	27260156	G402	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.43304854299519835	[221]	[579,287,383,241]	5	hSpCas9	negative selection	6930702
70579509	70579532	6	-	27260156	L33	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.36082681597738864	[172]	[268,97,134,164]	7	hSpCas9	negative selection	6987670
70566933	70566956	6	+	27260156	L33	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.2990128310014627	[724]	[905,394,475,938]	6	hSpCas9	negative selection	6987671
70588665	70588688	6	+	27260156	L33	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.005080379671352275	[388]	[323,149,278,428]	0	hSpCas9	negative selection	6987672
70566984	70567007	6	-	27260156	L33	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.21168376617521556	[545]	[671,283,342,616]	4	hSpCas9	negative selection	7051951
70588679	70588702	6	-	27260156	L33	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.24596707272611873	[956]	[974,590,661,1072]	5	hSpCas9	negative selection	7051952
70579494	70579517	6	+	27260156	L33	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.24786191985706463	[221]	[249,88,207,256]	5	hSpCas9	negative selection	7051953
70579509	70579532	6	-	27260156	K562	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.33465679239473345	[172]	[170,268,125,207]	4	hSpCas9	negative selection	7108921
70566933	70566956	6	+	27260156	K562	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.09796087328820613	[724]	[523,786,817,621]	1	hSpCas9	negative selection	7108922
70588665	70588688	6	+	27260156	K562	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.47363696314097425	[388]	[151,391,228,222]	-5	hSpCas9	negative selection	7108923
70566984	70567007	6	-	27260156	K562	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.1950189384537812	[545]	[462,765,408,578]	2	hSpCas9	negative selection	7173202
70588679	70588702	6	-	27260156	K562	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.8071160474081256	[956]	[2086,1690,1190,1076]	8	hSpCas9	negative selection	7173203
70579494	70579517	6	+	27260156	K562	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.43419487984536265	[221]	[205,332,339,191]	5	hSpCas9	negative selection	7173204
70579509	70579532	6	-	27260156	HT29	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.8036034687776481	[172]	[338,342,381,393]	9	hSpCas9	negative selection	7230172
70566933	70566956	6	+	27260156	HT29	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.35610916374221135	[724]	[1330,1161,1183,876]	7	hSpCas9	negative selection	7230173
70588665	70588688	6	+	27260156	HT29	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.12489172685449912	[388]	[662,538,550,340]	2	hSpCas9	negative selection	7230174
70566984	70567007	6	-	27260156	HT29	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.5040325676609332	[545]	[1031,998,975,782]	8	hSpCas9	negative selection	7294453
70588679	70588702	6	-	27260156	HT29	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.4512047831225299	[956]	[1598,1852,1406,1522]	8	hSpCas9	negative selection	7294454
70579494	70579517	6	+	27260156	HT29	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.3405649481167876	[221]	[266,398,323,369]	6	hSpCas9	negative selection	7294455
70579509	70579532	6	-	27260156	MHHES1	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.8912558781833793	[172]	[435,418,405,223]	9	hSpCas9	negative selection	7351423
70566933	70566956	6	+	27260156	MHHES1	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3560991835460763	[724]	[1083,1084,1034,976]	6	hSpCas9	negative selection	7351424
70588665	70588688	6	+	27260156	MHHES1	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.23908627419693673	[388]	[267,547,429,261]	-4	hSpCas9	negative selection	7351425
70566984	70567007	6	-	27260156	MHHES1	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.2455342457172719	[545]	[967,854,545,600]	4	hSpCas9	negative selection	7415704
70588679	70588702	6	-	27260156	MHHES1	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.30481049027766316	[956]	[1448,1701,935,1270]	5	hSpCas9	negative selection	7415705
70579494	70579517	6	+	27260156	MHHES1	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.5770061385159245	[221]	[302,304,524,342]	8	hSpCas9	negative selection	7415706
70579509	70579532	6	-	27260156	MEWO	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.3344420277908273	[172]	[314,137,180]	7	hSpCas9	negative selection	7472674
70566933	70566956	6	+	27260156	MEWO	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.5350386088048635	[724]	[1170,919,893]	9	hSpCas9	negative selection	7472675
70588665	70588688	6	+	27260156	MEWO	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.04313385539582121	[388]	[334,396,384]	1	hSpCas9	negative selection	7472676
70566984	70567007	6	-	27260156	MEWO	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.2749478584711326	[545]	[811,540,538]	6	hSpCas9	negative selection	7536955
70588679	70588702	6	-	27260156	MEWO	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.37627395742530356	[956]	[1281,1028,1198]	7	hSpCas9	negative selection	7536956
70579494	70579517	6	+	27260156	MEWO	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.22677526526792768	[221]	[314,248,178]	5	hSpCas9	negative selection	7536957
70579509	70579532	6	-	27260156	LNCAPCLONEFGC	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.6607092527799869	[172]	[178,278,280,255]	9	hSpCas9	negative selection	7593925
70566933	70566956	6	+	27260156	LNCAPCLONEFGC	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.4378490303385614	[724]	[713,896,1099,880]	7	hSpCas9	negative selection	7593926
70588665	70588688	6	+	27260156	LNCAPCLONEFGC	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.07623293010493848	[388]	[366,247,375,356]	-1	hSpCas9	negative selection	7593927
70566984	70567007	6	-	27260156	LNCAPCLONEFGC	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.08085081011604284	[545]	[564,394,652,505]	1	hSpCas9	negative selection	7658206
70588679	70588702	6	-	27260156	LNCAPCLONEFGC	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.3852669340548762	[956]	[1124,1051,1342,1044]	6	hSpCas9	negative selection	7658207
70579494	70579517	6	+	27260156	LNCAPCLONEFGC	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.4144933668106875	[221]	[176,344,310,241]	6	hSpCas9	negative selection	7658208
70579509	70579532	6	-	27260156	PANC0327	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.47402806278780774	[172]	[218,183,215,238]	7	hSpCas9	negative selection	7715176
70566933	70566956	6	+	27260156	PANC0327	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.2207444419150809	[724]	[685,682,1122,513]	3	hSpCas9	negative selection	7715177
70588665	70588688	6	+	27260156	PANC0327	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-1.041699711060314	[388]	[201,129,149,196]	-8	hSpCas9	negative selection	7715178
70566984	70567007	6	-	27260156	PANC0327	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	-0.06456119151443249	[545]	[526,470,329,530]	-1	hSpCas9	negative selection	7779457
70588679	70588702	6	-	27260156	PANC0327	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.24716643772799385	[956]	[759,956,1137,1166]	3	hSpCas9	negative selection	7779458
70579494	70579517	6	+	27260156	PANC0327	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	-0.19938133755088794	[221]	[150,73,264,207]	-3	hSpCas9	negative selection	7779459
70579509	70579532	6	-	27260156	NCIH2009	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.5685718920674386	[172]	[395,369,315]	7	hSpCas9	negative selection	7836427
70566933	70566956	6	+	27260156	NCIH2009	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.5598304044030136	[724]	[1762,1509,1256]	7	hSpCas9	negative selection	7836428
70588665	70588688	6	+	27260156	NCIH2009	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.42781943085070984	[388]	[513,410,307]	-5	hSpCas9	negative selection	7836429
70566984	70567007	6	-	27260156	NCIH2009	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.41808691120453123	[545]	[980,718,1353]	6	hSpCas9	negative selection	7900708
70588679	70588702	6	-	27260156	NCIH2009	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.12523492235878586	[956]	[1721,1217,1488]	1	hSpCas9	negative selection	7900709
70579494	70579517	6	+	27260156	NCIH2009	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.6483924224047017	[221]	[580,508,385]	8	hSpCas9	negative selection	7900710
70579509	70579532	6	-	27260156	NCIH1373	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	1.2163350628768006	[172]	[296,287,409,215]	9	hSpCas9	negative selection	7957678
70566933	70566956	6	+	27260156	NCIH1373	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.7349898334274179	[724]	[1799,958,1246,399]	7	hSpCas9	negative selection	7957679
70588665	70588688	6	+	27260156	NCIH1373	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.14097156609830463	[388]	[705,295,360,163]	1	hSpCas9	negative selection	7957680
70566984	70567007	6	-	27260156	NCIH1373	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.7517649849993676	[545]	[878,890,950,370]	7	hSpCas9	negative selection	8021959
70588679	70588702	6	-	27260156	NCIH1373	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.7506022866669007	[956]	[1405,2027,1889,471]	7	hSpCas9	negative selection	8021960
70579494	70579517	6	+	27260156	NCIH1373	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	-0.01930545137292805	[221]	[308,269,243,41]	0	hSpCas9	negative selection	8021961
70579509	70579532	6	-	27260156	PATU8902	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.47696636700313233	[172]	[506,133,288,428]	5	hSpCas9	negative selection	8078929
70566933	70566956	6	+	27260156	PATU8902	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.2405212438764055	[724]	[1515,931,882,1548]	2	hSpCas9	negative selection	8078930
70588665	70588688	6	+	27260156	PATU8902	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.4766350871529468	[388]	[251,226,604,418]	-5	hSpCas9	negative selection	8078931
70566984	70567007	6	-	27260156	PATU8902	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.33770010127382144	[545]	[885,1210,738,1072]	3	hSpCas9	negative selection	8143210
70588679	70588702	6	-	27260156	PATU8902	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.051977976035054274	[956]	[1440,1399,935,1917]	0	hSpCas9	negative selection	8143211
70579494	70579517	6	+	27260156	PATU8902	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.7070382921426799	[221]	[444,406,481,711]	7	hSpCas9	negative selection	8143212
70579509	70579532	6	-	27260156	PANC1	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.5188108257291268	[172]	[213,251,191,199]	8	hSpCas9	negative selection	8200180
70566933	70566956	6	+	27260156	PANC1	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3330718902731269	[724]	[731,924,785,721]	5	hSpCas9	negative selection	8200181
70588665	70588688	6	+	27260156	PANC1	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.022853008000454922	[388]	[441,398,168,348]	0	hSpCas9	negative selection	8200182
70566984	70567007	6	-	27260156	PANC1	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.10257597769188154	[545]	[598,745,467,277]	1	hSpCas9	negative selection	8264461
70588679	70588702	6	-	27260156	PANC1	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.4466113684565487	[956]	[1078,1370,1134,956]	7	hSpCas9	negative selection	8264462
70579494	70579517	6	+	27260156	PANC1	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	-0.07307840924296927	[221]	[154,160,143,246]	-1	hSpCas9	negative selection	8264463
70579509	70579532	6	-	27260156	PANC0813	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.3586668437689182	[172]	[221,241,338,236]	6	hSpCas9	negative selection	8321431
70566933	70566956	6	+	27260156	PANC0813	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.5787502507675653	[724]	[1076,1135,1357,1484]	8	hSpCas9	negative selection	8321432
70588665	70588688	6	+	27260156	PANC0813	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.36880509090615154	[388]	[259,283,337,514]	-5	hSpCas9	negative selection	8321433
70566984	70567007	6	-	27260156	PANC0813	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.4836603220766157	[545]	[728,1438,692,717]	7	hSpCas9	negative selection	8385712
70588679	70588702	6	-	27260156	PANC0813	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.18014069334533866	[956]	[1459,1308,1123,1218]	3	hSpCas9	negative selection	8385713
70579494	70579517	6	+	27260156	PANC0813	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.46731583465058757	[221]	[462,283,395,310]	7	hSpCas9	negative selection	8385714
70579509	70579532	6	-	27260156	RDES	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.6825855463448061	[172]	[221,301,314,475]	9	hSpCas9	negative selection	8442682
70566933	70566956	6	+	27260156	RDES	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.20522235682914902	[724]	[911,888,922,1130]	3	hSpCas9	negative selection	8442683
70588665	70588688	6	+	27260156	RDES	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.1156857640265107	[388]	[367,304,463,543]	-2	hSpCas9	negative selection	8442684
70566984	70567007	6	-	27260156	RDES	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.1984876415703078	[545]	[562,580,775,1039]	3	hSpCas9	negative selection	8506963
70588679	70588702	6	-	27260156	RDES	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.42570045279321955	[956]	[1259,1314,1885,1485]	6	hSpCas9	negative selection	8506964
70579494	70579517	6	+	27260156	RDES	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.6094894248673263	[221]	[330,395,360,482]	8	hSpCas9	negative selection	8506965
70579509	70579532	6	-	27260156	PC3	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.6416042169078922	[172]	[190,260,350,311]	9	hSpCas9	negative selection	8563933
70566933	70566956	6	+	27260156	PC3	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.06059134863271309	[724]	[568,677,1272,692]	1	hSpCas9	negative selection	8563934
70588665	70588688	6	+	27260156	PC3	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.16317870312638255	[388]	[222,365,911,485]	3	hSpCas9	negative selection	8563935
70566984	70567007	6	-	27260156	PC3	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	-0.37752506608277636	[545]	[340,346,747,351]	-7	hSpCas9	negative selection	8628214
70588679	70588702	6	-	27260156	PC3	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.26468227561609337	[956]	[725,954,2273,1152]	6	hSpCas9	negative selection	8628215
70579494	70579517	6	+	27260156	PC3	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.4283023784198394	[221]	[188,207,734,251]	8	hSpCas9	negative selection	8628216
70579509	70579532	6	-	27260156	PATU8988T	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	1.0506829507433442	[172]	[315,380,123,589]	9	hSpCas9	negative selection	8685184
70566933	70566956	6	+	27260156	PATU8988T	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.4195551213048523	[724]	[1039,884,791,1035]	7	hSpCas9	negative selection	8685185
70588665	70588688	6	+	27260156	PATU8988T	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.46648722038614365	[388]	[416,816,486,372]	7	hSpCas9	negative selection	8685186
70566984	70567007	6	-	27260156	PATU8988T	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.3207231393294323	[545]	[600,796,538,717]	5	hSpCas9	negative selection	8749465
70588679	70588702	6	-	27260156	PATU8988T	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	-0.051936638134109536	[956]	[734,795,943,1099]	-1	hSpCas9	negative selection	8749466
70579494	70579517	6	+	27260156	PATU8988T	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.7376137897974577	[221]	[513,366,261,283]	9	hSpCas9	negative selection	8749467
70579509	70579532	6	-	27260156	T47D	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.7443733743814117	[172]	[306,216,522,309]	9	hSpCas9	negative selection	8806435
70566933	70566956	6	+	27260156	T47D	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.23765403211431746	[724]	[964,1005,1030,978]	5	hSpCas9	negative selection	8806436
70588665	70588688	6	+	27260156	T47D	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.07046828043837206	[388]	[416,380,406,528]	-1	hSpCas9	negative selection	8806437
70566984	70567007	6	-	27260156	T47D	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.162357469258472	[545]	[713,724,639,769]	3	hSpCas9	negative selection	8870716
70588679	70588702	6	-	27260156	T47D	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.3121779977394541	[956]	[1445,1291,1444,1359]	6	hSpCas9	negative selection	8870717
70579494	70579517	6	+	27260156	T47D	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.29881136546991194	[221]	[373,276,336,286]	6	hSpCas9	negative selection	8870718
70579509	70579532	6	-	27260156	SU8686	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.7557428398485443	[172]	[266,239,299,371]	9	hSpCas9	negative selection	8927686
70566933	70566956	6	+	27260156	SU8686	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.6456281100307868	[724]	[1072,933,1245,1298]	9	hSpCas9	negative selection	8927687
70588665	70588688	6	+	27260156	SU8686	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.12076333826595359	[388]	[287,326,499,308]	-2	hSpCas9	negative selection	8927688
70566984	70567007	6	-	27260156	SU8686	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.0896401560464552	[545]	[482,487,659,715]	1	hSpCas9	negative selection	8991967
70588679	70588702	6	-	27260156	SU8686	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.4071674333945785	[956]	[1110,966,1413,1654]	7	hSpCas9	negative selection	8991968
70579494	70579517	6	+	27260156	SU8686	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.6527825957725725	[221]	[345,352,212,487]	9	hSpCas9	negative selection	8991969
70579509	70579532	6	-	27260156	SKES1	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.6415474637914497	[172]	[362,337,176,223]	9	hSpCas9	negative selection	9048937
70566933	70566956	6	+	27260156	SKES1	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	-0.12584886377218313	[724]	[769,750,654,511]	-3	hSpCas9	negative selection	9048938
70588665	70588688	6	+	27260156	SKES1	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.19483117088987983	[388]	[394,532,494,361]	4	hSpCas9	negative selection	9048939
70566984	70567007	6	-	27260156	SKES1	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.051891228477623175	[545]	[747,591,538,419]	1	hSpCas9	negative selection	9113218
70588679	70588702	6	-	27260156	SKES1	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.18383865413055567	[956]	[1136,1083,1257,876]	4	hSpCas9	negative selection	9113219
70579494	70579517	6	+	27260156	SKES1	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.118042498865494	[221]	[258,336,154,224]	2	hSpCas9	negative selection	9113220
70579509	70579532	6	-	27260156	TOV112D	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.8682917448561598	[172]	[224,630,254,225]	9	hSpCas9	negative selection	9170188
70566933	70566956	6	+	27260156	TOV112D	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.34662187344003714	[724]	[979,1485,843,535]	6	hSpCas9	negative selection	9170189
70588665	70588688	6	+	27260156	TOV112D	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.1059833844310063	[388]	[206,381,404,442]	-2	hSpCas9	negative selection	9170190
70566984	70567007	6	-	27260156	TOV112D	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.002744805087265434	[545]	[444,828,495,482]	0	hSpCas9	negative selection	9234469
70588679	70588702	6	-	27260156	TOV112D	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.35458717954688734	[956]	[1357,1570,1048,1023]	6	hSpCas9	negative selection	9234470
70579494	70579517	6	+	27260156	TOV112D	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.31700080926309604	[221]	[390,425,213,126]	6	hSpCas9	negative selection	9234471
70579509	70579532	6	-	27260156	TC71	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.38775707369821855	[172]	[186,299,189,299]	8	hSpCas9	negative selection	9291439
70566933	70566956	6	+	27260156	TC71	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.3521802347107703	[724]	[947,1263,873,879]	8	hSpCas9	negative selection	9291440
70588665	70588688	6	+	27260156	TC71	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	0.1924786940589377	[388]	[428,560,482,430]	5	hSpCas9	negative selection	9291441
70566984	70567007	6	-	27260156	TC71	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	0.22025334078629016	[545]	[603,681,712,731]	5	hSpCas9	negative selection	9355720
70588679	70588702	6	-	27260156	TC71	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.33504395213419813	[956]	[1279,1335,1218,1346]	7	hSpCas9	negative selection	9355721
70579494	70579517	6	+	27260156	TC71	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.1949948987773723	[221]	[265,272,253,298]	5	hSpCas9	negative selection	9355722
70579509	70579532	6	-	27260156	TC32	viability	GGTGCATCAAAACGACCTTCTGG	SDHAF4	ENSG00000154079	0.2907952116095889	[172]	[226,100,204,290]	5	hSpCas9	negative selection	9412690
70566933	70566956	6	+	27260156	TC32	viability	TCGGCGCCATGACCCCATCGAGG	SDHAF4	ENSG00000154079	0.1748763117069586	[724]	[638,734,812,883]	3	hSpCas9	negative selection	9412691
70588665	70588688	6	+	27260156	TC32	viability	GGCCCAGAACCTACCCGATATGG	SDHAF4	ENSG00000154079	-0.05460227982913246	[388]	[333,217,415,491]	-1	hSpCas9	negative selection	9412692
70566984	70567007	6	-	27260156	TC32	viability	ACTTGCCGCTCTCCACGCCGTGG	SDHAF4	ENSG00000154079	-0.008428311630658436	[545]	[379,448,668,582]	0	hSpCas9	negative selection	9476971
70588679	70588702	6	-	27260156	TC32	viability	TTCGTTCCCAATCTCCATATCGG	SDHAF4	ENSG00000154079	0.21992211358324165	[956]	[842,827,1383,1251]	4	hSpCas9	negative selection	9476972
70579494	70579517	6	+	27260156	TC32	viability	AAGAAGCCGAAGTTACCAGAAGG	SDHAF4	ENSG00000154079	0.2839772376400179	[221]	[240,240,317,214]	5	hSpCas9	negative selection	9476973