Crispr

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 6683
• Symbol: SPAST
• Description: spastin

DataSource: http://genomecrispr.dkfz.de/#!/results/SPAST

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
32063853	32063876	2	+	26472758	Jiyoye	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	0.1067804481681525	[132]	[107]	0	hSpCas9	negative selection	154475
32087509	32087532	2	+	26472758	Jiyoye	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.6792549269426743	[52]	[63]	5	hSpCas9	negative selection	154476
32063992	32064015	2	-	26472758	Jiyoye	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-0.29808135293299465	[74]	[45]	-2	hSpCas9	negative selection	154477
32064068	32064091	2	-	26472758	Jiyoye	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-0.9262483522193184	[62]	[24]	-6	hSpCas9	negative selection	154478
32136567	32136590	2	+	26472758	Jiyoye	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-1.9089703607874828	[248]	[49]	-8	hSpCas9	negative selection	154479
32064041	32064064	2	-	26472758	Jiyoye	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.5636782728346101	[195]	[99]	-4	hSpCas9	negative selection	154480
32141925	32141948	2	+	26472758	Jiyoye	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-1.8116592204173854	[269]	[57]	-8	hSpCas9	negative selection	154481
32144986	32145009	2	+	26472758	Jiyoye	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	0.3719859530435735	[82]	[80]	3	hSpCas9	negative selection	154482
32064199	32064222	2	-	26472758	Jiyoye	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-0.29584288073699494	[139]	[85]	-2	hSpCas9	negative selection	154483
32087493	32087516	2	+	26472758	Jiyoye	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.6556654920643581	[304]	[145]	-5	hSpCas9	negative selection	154484
32063853	32063876	2	+	26472758	KBM7	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.4744087695606567	[339,225]	[126,64]	-4	hSpCas9	negative selection	345593
32087509	32087532	2	+	26472758	KBM7	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.0144003513154137	[338,460]	[309,89]	0	hSpCas9	negative selection	345594
32063992	32064015	2	-	26472758	KBM7	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.09437546013661069	[239,161]	[110,88]	0	hSpCas9	negative selection	345595
32064068	32064091	2	-	26472758	KBM7	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	0.16432508026771764	[155,110]	[69,68]	1	hSpCas9	negative selection	345596
32136567	32136590	2	+	26472758	KBM7	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-2.062863772427685	[1048,732]	[118,80]	-8	hSpCas9	negative selection	345597
32064041	32064064	2	-	26472758	KBM7	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.4310212000694752	[640,484]	[212,177]	-4	hSpCas9	negative selection	345598
32141925	32141948	2	+	26472758	KBM7	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-0.1818821013143609	[895,787]	[283,405]	-2	hSpCas9	negative selection	345599
32144986	32145009	2	+	26472758	KBM7	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-0.24254454671655612	[476,242]	[100,172]	-2	hSpCas9	negative selection	345600
32064199	32064222	2	-	26472758	KBM7	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-0.030787188457932817	[524,438]	[269,176]	0	hSpCas9	negative selection	345601
32087493	32087516	2	+	26472758	KBM7	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.4217519690744469	[890,1164]	[357,375]	-4	hSpCas9	negative selection	345602
32063853	32063876	2	+	26472758	Raji	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	0.13629622728883847	[246]	[62]	1	hSpCas9	negative selection	536711
32087509	32087532	2	+	26472758	Raji	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.8064295695799788	[238]	[96]	7	hSpCas9	negative selection	536712
32063992	32064015	2	-	26472758	Raji	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-2.3076539639052442	[127]	[5]	-9	hSpCas9	negative selection	536713
32064068	32064091	2	-	26472758	Raji	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-2.046421800705436	[88]	[4]	-8	hSpCas9	negative selection	536714
32136567	32136590	2	+	26472758	Raji	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-1.2292660652205283	[393]	[38]	-7	hSpCas9	negative selection	536715
32064041	32064064	2	-	26472758	Raji	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.2525124097127833	[307]	[29]	-7	hSpCas9	negative selection	536716
32141925	32141948	2	+	26472758	Raji	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	0.3125738666351089	[457]	[131]	3	hSpCas9	negative selection	536717
32144986	32145009	2	+	26472758	Raji	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	0.3071530477493767	[201]	[57]	3	hSpCas9	negative selection	536718
32064199	32064222	2	-	26472758	Raji	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-1.0442096432964556	[390]	[43]	-6	hSpCas9	negative selection	536719
32087493	32087516	2	+	26472758	Raji	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	0.7990750497753649	[368]	[148]	7	hSpCas9	negative selection	536720
32063853	32063876	2	+	26472758	K562	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-1.102503746687785	[261]	[102]	-6	hSpCas9	negative selection	727829
32087509	32087532	2	+	26472758	K562	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.9813843218326529	[398]	[664]	8	hSpCas9	negative selection	727830
32063992	32064015	2	-	26472758	K562	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	1.2152723820069302	[74]	[146]	8	hSpCas9	negative selection	727831
32064068	32064091	2	-	26472758	K562	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-0.5358000644536434	[78]	[45]	-3	hSpCas9	negative selection	727832
32136567	32136590	2	+	26472758	K562	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-3.3948134355828303	[622]	[49]	-8	hSpCas9	negative selection	727833
32064041	32064064	2	-	26472758	K562	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.0953084351618314	[366]	[144]	-6	hSpCas9	negative selection	727834
32141925	32141948	2	+	26472758	K562	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-0.3877462944536086	[698]	[450]	-3	hSpCas9	negative selection	727835
32144986	32145009	2	+	26472758	K562	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-0.3303839068344876	[282]	[189]	-2	hSpCas9	negative selection	727836
32064199	32064222	2	-	26472758	K562	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	0.6415343189480284	[242]	[319]	5	hSpCas9	negative selection	727837
32087493	32087516	2	+	26472758	K562	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	0.6716238593975306	[855]	[1150]	5	hSpCas9	negative selection	727838
32063997	32064020	2	-	26627737	DLD1	viability	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.1956532071413284	[1289]	[314,413,332]	-2	hSpCas9	negative selection	811738
32064220	32064243	2	-	26627737	DLD1	viability	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.7587510985727846	[452]	[195,419,127]	7	hSpCas9	negative selection	811739
32098855	32098878	2	+	26627737	DLD1	viability	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.3334159386959778	[352]	[121,108,39]	-3	hSpCas9	negative selection	811740
32114745	32114768	2	+	26627737	DLD1	viability	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.19151364358228162	[747]	[254,374,187]	2	hSpCas9	negative selection	811741
32114773	32114796	2	-	26627737	DLD1	viability	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.24566541854415302	[820]	[124,261,257]	-3	hSpCas9	negative selection	811742
32154451	32154474	2	+	26627737	DLD1	viability	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.07739798252164132	[1209]	[516,360,218]	-1	hSpCas9	negative selection	811743
32063997	32064020	2	-	26627737	GBM cells	viability after 5 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.45093292356665016	[886]	[322,372]	-6	hSpCas9	negative selection	894053
32064220	32064243	2	-	26627737	GBM cells	viability after 5 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.15417093012776012	[278]	[155,176]	2	hSpCas9	negative selection	894054
32098855	32098878	2	+	26627737	GBM cells	viability after 5 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.08487589793305561	[176]	[87,90]	-1	hSpCas9	negative selection	894055
32114745	32114768	2	+	26627737	GBM cells	viability after 5 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.46134233866077634	[533]	[204,210]	-6	hSpCas9	negative selection	894056
32114773	32114796	2	-	26627737	GBM cells	viability after 5 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.013718350376486166	[494]	[256,278]	0	hSpCas9	negative selection	894057
32154451	32154474	2	+	26627737	GBM cells	viability after 5 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.17721503737278987	[704]	[420,433]	3	hSpCas9	negative selection	894058
32063997	32064020	2	-	26627737	GBM cells	viability after 13 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.6344943280279591	[886]	[341,283]	-6	hSpCas9	negative selection	976368
32064220	32064243	2	-	26627737	GBM cells	viability after 13 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.17339795321239782	[278]	[120,144]	-2	hSpCas9	negative selection	976369
32098855	32098878	2	+	26627737	GBM cells	viability after 13 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.41705151841709975	[176]	[61,79]	-5	hSpCas9	negative selection	976370
32114745	32114768	2	+	26627737	GBM cells	viability after 13 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.5034140817773736	[533]	[226,185]	-5	hSpCas9	negative selection	976371
32114773	32114796	2	-	26627737	GBM cells	viability after 13 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.03603366559461163	[494]	[324,234]	0	hSpCas9	negative selection	976372
32154451	32154474	2	+	26627737	GBM cells	viability after 13 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.38844327054314176	[704]	[610,410]	5	hSpCas9	negative selection	976373
32063997	32064020	2	-	26627737	GBM cells	viability after 21 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.3828661672134739	[886]	[414,327]	-3	hSpCas9	negative selection	1058683
32064220	32064243	2	-	26627737	GBM cells	viability after 21 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.3751683270625095	[278]	[119,113]	-3	hSpCas9	negative selection	1058684
32098855	32098878	2	+	26627737	GBM cells	viability after 21 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.4601516999792652	[176]	[83,56]	-4	hSpCas9	negative selection	1058685
32114745	32114768	2	+	26627737	GBM cells	viability after 21 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.45761716070324654	[533]	[216,205]	-4	hSpCas9	negative selection	1058686
32114773	32114796	2	-	26627737	GBM cells	viability after 21 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.20434887523477774	[494]	[336,284]	2	hSpCas9	negative selection	1058687
32154451	32154474	2	+	26627737	GBM cells	viability after 21 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.627503028276138	[704]	[621,563]	6	hSpCas9	negative selection	1058688
32063997	32064020	2	-	26627737	RPE1	viability after 9 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.14941669216319253	[2293]	[634,1004]	1	hSpCas9	negative selection	1140998
32064220	32064243	2	-	26627737	RPE1	viability after 9 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	3.103581370307777	[99]	[100,485]	9	hSpCas9	negative selection	1140999
32098855	32098878	2	+	26627737	RPE1	viability after 9 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-2.4183518702303632	[764]	[39,50]	-9	hSpCas9	negative selection	1141000
32114745	32114768	2	+	26627737	RPE1	viability after 9 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.0679768354944772	[1888]	[554,575]	0	hSpCas9	negative selection	1141001
32114773	32114796	2	-	26627737	RPE1	viability after 9 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.8809563764783258	[935]	[519,564]	5	hSpCas9	negative selection	1141002
32154451	32154474	2	+	26627737	RPE1	viability after 9 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	1.13311676235899	[1529]	[1064,1031]	7	hSpCas9	negative selection	1141003
32063997	32064020	2	-	26627737	RPE1	viability after 12 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.3395806500306364	[2293]	[792,765]	2	hSpCas9	negative selection	1223313
32064220	32064243	2	-	26627737	RPE1	viability after 12 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	3.3347084367809026	[99]	[153,394]	9	hSpCas9	negative selection	1223314
32098855	32098878	2	+	26627737	RPE1	viability after 12 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-1.461548361305412	[764]	[95,51]	-7	hSpCas9	negative selection	1223315
32114745	32114768	2	+	26627737	RPE1	viability after 12 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.1668715476186493	[1888]	[645,488]	1	hSpCas9	negative selection	1223316
32114773	32114796	2	-	26627737	RPE1	viability after 12 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.6824269222220416	[935]	[320,490]	4	hSpCas9	negative selection	1223317
32154451	32154474	2	+	26627737	RPE1	viability after 12 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	1.2134738584096252	[1529]	[994,908]	7	hSpCas9	negative selection	1223318
32063997	32064020	2	-	26627737	RPE1	viability after 15 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.07357332068862199	[2293]	[689,675]	0	hSpCas9	negative selection	1305628
32064220	32064243	2	-	26627737	RPE1	viability after 15 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	2.4374335965581233	[99]	[55,246]	9	hSpCas9	negative selection	1305629
32098855	32098878	2	+	26627737	RPE1	viability after 15 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-1.5818961583101019	[764]	[27,114]	-7	hSpCas9	negative selection	1305630
32114745	32114768	2	+	26627737	RPE1	viability after 15 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.34601339101472206	[1888]	[780,580]	2	hSpCas9	negative selection	1305631
32114773	32114796	2	-	26627737	RPE1	viability after 15 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.7956745355100089	[935]	[452,465]	5	hSpCas9	negative selection	1305632
32154451	32154474	2	+	26627737	RPE1	viability after 15 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	1.1292536802480306	[1529]	[848,1040]	6	hSpCas9	negative selection	1305633
32063997	32064020	2	-	26627737	RPE1	viability after 18 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.4616347599734272	[2293]	[736,975]	3	hSpCas9	negative selection	1387943
32064220	32064243	2	-	26627737	RPE1	viability after 18 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	2.7015483088907946	[99]	[176,173]	9	hSpCas9	negative selection	1387944
32098855	32098878	2	+	26627737	RPE1	viability after 18 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.7231524766103324	[764]	[155,91]	-4	hSpCas9	negative selection	1387945
32114745	32114768	2	+	26627737	RPE1	viability after 18 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.11654355997108401	[1888]	[615,484]	0	hSpCas9	negative selection	1387946
32114773	32114796	2	-	26627737	RPE1	viability after 18 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.8428217090318121	[935]	[366,544]	5	hSpCas9	negative selection	1387947
32154451	32154474	2	+	26627737	RPE1	viability after 18 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	1.387611017453033	[1529]	[885,1287]	7	hSpCas9	negative selection	1387948
32063997	32064020	2	-	26627737	HeLa	viability after 8 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.04607885777385112	[1263]	[808,547,410]	0	hSpCas9	negative selection	1470258
32064220	32064243	2	-	26627737	HeLa	viability after 8 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.26080339357950694	[510]	[357,203,257]	2	hSpCas9	negative selection	1470259
32098855	32098878	2	+	26627737	HeLa	viability after 8 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	1.0561562505943878	[213]	[217,244,127]	7	hSpCas9	negative selection	1470260
32114745	32114768	2	+	26627737	HeLa	viability after 8 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.32374035893406916	[741]	[294,242,244]	-3	hSpCas9	negative selection	1470261
32114773	32114796	2	-	26627737	HeLa	viability after 8 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.06375120831034176	[785]	[382,440,260]	0	hSpCas9	negative selection	1470262
32154451	32154474	2	+	26627737	HeLa	viability after 8 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.32472391667066103	[1588]	[390,621,603]	-3	hSpCas9	negative selection	1470263
32063997	32064020	2	-	26627737	HeLa	viability after 12 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.4475710683937195	[1263]	[537,303,319]	-4	hSpCas9	negative selection	1552573
32064220	32064243	2	-	26627737	HeLa	viability after 12 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.0801419421360463	[510]	[216,133,327]	0	hSpCas9	negative selection	1552574
32098855	32098878	2	+	26627737	HeLa	viability after 12 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.27564467096627177	[213]	[34,101,77]	-2	hSpCas9	negative selection	1552575
32114745	32114768	2	+	26627737	HeLa	viability after 12 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.004086975496385037	[741]	[326,191,410]	0	hSpCas9	negative selection	1552576
32114773	32114796	2	-	26627737	HeLa	viability after 12 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.5009284364135256	[785]	[293,186,213]	-4	hSpCas9	negative selection	1552577
32154451	32154474	2	+	26627737	HeLa	viability after 12 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.33681120876455917	[1588]	[615,459,492]	-3	hSpCas9	negative selection	1552578
32063997	32064020	2	-	26627737	HeLa	viability after 15 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.18964855323564644	[1263]	[688,300,352]	-2	hSpCas9	negative selection	1634888
32064220	32064243	2	-	26627737	HeLa	viability after 15 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.3452894175271874	[510]	[123,208,477]	3	hSpCas9	negative selection	1634889
32098855	32098878	2	+	26627737	HeLa	viability after 15 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.7541123790641053	[213]	[28,209,207]	6	hSpCas9	negative selection	1634890
32114745	32114768	2	+	26627737	HeLa	viability after 15 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.33803064971503194	[741]	[362,469,306]	3	hSpCas9	negative selection	1634891
32114773	32114796	2	-	26627737	HeLa	viability after 15 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.6168749765008379	[785]	[316,141,161]	-5	hSpCas9	negative selection	1634892
32154451	32154474	2	+	26627737	HeLa	viability after 15 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.39081912407960506	[1588]	[280,549,664]	-3	hSpCas9	negative selection	1634893
32063997	32064020	2	-	26627737	HeLa	viability after 18 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.1907755305919392	[1263]	[464,244,352]	-2	hSpCas9	negative selection	1717203
32064220	32064243	2	-	26627737	HeLa	viability after 18 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.37185623674297047	[510]	[87,178,477]	3	hSpCas9	negative selection	1717204
32098855	32098878	2	+	26627737	HeLa	viability after 18 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.7370192816535677	[213]	[19,168,207]	6	hSpCas9	negative selection	1717205
32114745	32114768	2	+	26627737	HeLa	viability after 18 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.3351161941258215	[741]	[242,387,306]	3	hSpCas9	negative selection	1717206
32114773	32114796	2	-	26627737	HeLa	viability after 18 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.5657537297635333	[785]	[226,117,161]	-5	hSpCas9	negative selection	1717207
32154451	32154474	2	+	26627737	HeLa	viability after 18 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.409692700445182	[1588]	[169,462,664]	-4	hSpCas9	negative selection	1717208
32063997	32064020	2	-	26627737	HCT116	viability after 6 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.01377100005435894	[2146]	[294,355]	0	hSpCas9	negative selection	1799518
32064220	32064243	2	-	26627737	HCT116	viability after 6 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	1.3519506240556207	[1095]	[1220,8]	7	hSpCas9	negative selection	1799519
32098855	32098878	2	+	26627737	HCT116	viability after 6 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	1.637586269112215	[243]	[284,27]	8	hSpCas9	negative selection	1799520
32114745	32114768	2	+	26627737	HCT116	viability after 6 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.936319521024152	[949]	[580,122]	5	hSpCas9	negative selection	1799521
32114773	32114796	2	-	26627737	HCT116	viability after 6 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.13954875437367237	[833]	[253,83]	0	hSpCas9	negative selection	1799522
32154451	32154474	2	+	26627737	HCT116	viability after 6 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.5033472950694164	[1568]	[387,55]	-3	hSpCas9	negative selection	1799523
32063997	32064020	2	-	26627737	HCT116	viability after 8 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.06744305943479478	[691]	[373,323,498]	0	hSpCas9	negative selection	1881833
32064220	32064243	2	-	26627737	HCT116	viability after 8 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.4571094085055235	[386]	[135,118,209]	-5	hSpCas9	negative selection	1881834
32098855	32098878	2	+	26627737	HCT116	viability after 8 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.6361265441685842	[137]	[217,75,62]	6	hSpCas9	negative selection	1881835
32114745	32114768	2	+	26627737	HCT116	viability after 8 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.31617815340559696	[656]	[219,354,295]	-4	hSpCas9	negative selection	1881836
32114773	32114796	2	-	26627737	HCT116	viability after 8 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.3699223649568467	[447]	[354,309,292]	4	hSpCas9	negative selection	1881837
32154451	32154474	2	+	26627737	HCT116	viability after 8 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.019897409701512103	[835]	[531,424,407]	0	hSpCas9	negative selection	1881838
32063997	32064020	2	-	26627737	HCT116	viability after 9 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.7978188398227815	[2146]	[517,180]	-5	hSpCas9	negative selection	1964148
32064220	32064243	2	-	26627737	HCT116	viability after 9 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.036036340525574584	[1095]	[289,307]	0	hSpCas9	negative selection	1964149
32098855	32098878	2	+	26627737	HCT116	viability after 9 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.6415718290453395	[243]	[85,113]	4	hSpCas9	negative selection	1964150
32114745	32114768	2	+	26627737	HCT116	viability after 9 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.4144315836053034	[949]	[508,202]	3	hSpCas9	negative selection	1964151
32114773	32114796	2	-	26627737	HCT116	viability after 9 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.27563757433350716	[833]	[368,190]	2	hSpCas9	negative selection	1964152
32154451	32154474	2	+	26627737	HCT116	viability after 9 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.4891430358442215	[1568]	[620,561]	3	hSpCas9	negative selection	1964153
32063997	32064020	2	-	26627737	HCT116	viability after 12 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.234972810201016	[691]	[450,429,400]	2	hSpCas9	negative selection	2046463
32064220	32064243	2	-	26627737	HCT116	viability after 12 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.2865644302550208	[386]	[227,166,348]	3	hSpCas9	negative selection	2046464
32098855	32098878	2	+	26627737	HCT116	viability after 12 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.7305559742631753	[137]	[199,119,33]	6	hSpCas9	negative selection	2046465
32114745	32114768	2	+	26627737	HCT116	viability after 12 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.4793047652137331	[656]	[163,273,312]	-5	hSpCas9	negative selection	2046466
32114773	32114796	2	-	26627737	HCT116	viability after 12 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.03804050747801169	[447]	[271,221,189]	0	hSpCas9	negative selection	2046467
32154451	32154474	2	+	26627737	HCT116	viability after 12 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.1292285031338536	[835]	[363,448,637]	1	hSpCas9	negative selection	2046468
32063997	32064020	2	-	26627737	HCT116	viability after 15 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.09325548710203835	[691]	[466,378,430]	1	hSpCas9	negative selection	2128778
32064220	32064243	2	-	26627737	HCT116	viability after 15 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.15548734685469778	[386]	[199,141,255]	-2	hSpCas9	negative selection	2128779
32098855	32098878	2	+	26627737	HCT116	viability after 15 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.544416548834103	[137]	[234,69,56]	5	hSpCas9	negative selection	2128780
32114745	32114768	2	+	26627737	HCT116	viability after 15 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.6310225942081933	[656]	[337,293,111]	-6	hSpCas9	negative selection	2128781
32114773	32114796	2	-	26627737	HCT116	viability after 15 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.4013652146293236	[447]	[476,208,349]	4	hSpCas9	negative selection	2128782
32154451	32154474	2	+	26627737	HCT116	viability after 15 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.29156451137876527	[835]	[279,734,708]	3	hSpCas9	negative selection	2128783
32063997	32064020	2	-	26627737	HCT116	viability after 18 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.7309763463277323	[691]	[549,572,540]	6	hSpCas9	negative selection	2211093
32064220	32064243	2	-	26627737	HCT116	viability after 18 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.0855944909928319	[386]	[181,218,192]	0	hSpCas9	negative selection	2211094
32098855	32098878	2	+	26627737	HCT116	viability after 18 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	1.357376544043129	[137]	[230,178,98]	8	hSpCas9	negative selection	2211095
32114745	32114768	2	+	26627737	HCT116	viability after 18 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.4141317558744958	[656]	[177,246,291]	-4	hSpCas9	negative selection	2211096
32114773	32114796	2	-	26627737	HCT116	viability after 18 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.1910571406889251	[447]	[251,268,217]	1	hSpCas9	negative selection	2211097
32154451	32154474	2	+	26627737	HCT116	viability after 18 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.30080387655301677	[835]	[394,450,657]	2	hSpCas9	negative selection	2211098
32063822	32063845	2	+	24336569	HL60	viability	AGAGCTGTGAATGAATTCTCCGG	SPAST	ENSG00000021574	-0.21074711757386289	[384]	[703]	-2	hSpCas9	negative selection	2296871
32063832	32063855	2	+	24336569	HL60	viability	ATGAATTCTCCGGGTGGACGAGG	SPAST	ENSG00000021574	-0.13591188465127274	[620]	[1195]	-1	hSpCas9	negative selection	2296872
32063841	32063864	2	-	24336569	HL60	viability	TTCTTCTTCCCTCGTCCACCCGG	SPAST	ENSG00000021574	-1.0100497673367337	[334]	[351]	-7	hSpCas9	negative selection	2296873
32063850	32063873	2	+	24336569	HL60	viability	CGAGGGAAGAAGAAAGGCTCCGG	SPAST	ENSG00000021574	-0.04597501591027131	[260]	[534]	0	hSpCas9	negative selection	2296874
32063867	32063890	2	+	24336569	HL60	viability	CTCCGGCGGCGCCAGCAACCCGG	SPAST	ENSG00000021574	-0.09676323701177897	[710]	[1406]	-1	hSpCas9	negative selection	2296875
32063878	32063901	2	+	24336569	HL60	viability	CCAGCAACCCGGTGCCTCCCAGG	SPAST	ENSG00000021574	0.8717127573253419	[273]	[1060]	7	hSpCas9	negative selection	2296876
32063885	32063908	2	-	24336569	HL60	viability	GCGGAGGCCTGGGAGGCACCGGG	SPAST	ENSG00000021574	-2.5331600704865886	[836]	[305]	-9	hSpCas9	negative selection	2296877
32063948	32063971	2	-	24336569	HL60	viability	TATGCGGCGACTCGGGCGGAGGG	SPAST	ENSG00000021574	-0.0487834018342333	[370]	[758]	0	hSpCas9	negative selection	2296878
32063953	32063976	2	+	24336569	HL60	viability	CGCCCGAGTCGCCGCATAAGCGG	SPAST	ENSG00000021574	2.1940150715715427	[100]	[977]	9	hSpCas9	negative selection	2296879
32063964	32063987	2	-	24336569	HL60	viability	TAGTACAGGTTCCGCTTATGCGG	SPAST	ENSG00000021574	-0.00870775819358216	[376]	[792]	0	hSpCas9	negative selection	2296880
32063822	32063845	2	+	24336569	KBM7	viability	AGAGCTGTGAATGAATTCTCCGG	SPAST	ENSG00000021574	-1.1447153632884417	[517]	[638]	-7	hSpCas9	negative selection	2364048
32063832	32063855	2	+	24336569	KBM7	viability	ATGAATTCTCCGGGTGGACGAGG	SPAST	ENSG00000021574	0.838047199928748	[329]	[1608]	7	hSpCas9	negative selection	2364049
32063841	32063864	2	-	24336569	KBM7	viability	TTCTTCTTCCCTCGTCCACCCGG	SPAST	ENSG00000021574	0.1265967489460369	[178]	[532]	1	hSpCas9	negative selection	2364050
32063850	32063873	2	+	24336569	KBM7	viability	CGAGGGAAGAAGAAAGGCTCCGG	SPAST	ENSG00000021574	-0.16685866798775384	[276]	[672]	-1	hSpCas9	negative selection	2364051
32063867	32063890	2	+	24336569	KBM7	viability	CTCCGGCGGCGCCAGCAACCCGG	SPAST	ENSG00000021574	1.0914975336533213	[377]	[2196]	8	hSpCas9	negative selection	2364052
32063878	32063901	2	+	24336569	KBM7	viability	CCAGCAACCCGGTGCCTCCCAGG	SPAST	ENSG00000021574	1.4036019664733486	[87]	[634]	9	hSpCas9	negative selection	2364053
32063885	32063908	2	-	24336569	KBM7	viability	GCGGAGGCCTGGGAGGCACCGGG	SPAST	ENSG00000021574	-1.3882006494699508	[594]	[619]	-7	hSpCas9	negative selection	2364054
32063948	32063971	2	-	24336569	KBM7	viability	TATGCGGCGACTCGGGCGGAGGG	SPAST	ENSG00000021574	0.1927919993785317	[314]	[981]	1	hSpCas9	negative selection	2364055
32063953	32063976	2	+	24336569	KBM7	viability	CGCCCGAGTCGCCGCATAAGCGG	SPAST	ENSG00000021574	1.5524208034511173	[70]	[567]	9	hSpCas9	negative selection	2364056
32063964	32063987	2	-	24336569	KBM7	viability	TAGTACAGGTTCCGCTTATGCGG	SPAST	ENSG00000021574	0.6188877122094869	[227]	[954]	5	hSpCas9	negative selection	2364057
32107478	32107501	2	-	25494202	A375	resistance to PLX-4720 (puromycin)	CAGGTGGATCACTTAAGGTCAGG	SPAST	ENSG00000021574	3.066970511819436	[16,22]	[18,57]	8	dCas9-VP64	positive selection	2389578
32068178	32068201	2	-	25494202	A375	resistance to PLX-4720 (puromycin)	CGAGACCATCCTGGCTAACACAG	SPAST	ENSG00000021574	-0.7007848477191231	[357,35]	[27,23]	-4	dCas9-VP64	positive selection	2404357
32146443	32146466	2	+	25494202	A375	resistance to PLX-4720 (puromycin)	CCCAGCTACTCGGGAGTCTGAAG	SPAST	ENSG00000021574	0.48846568775758525	[12,30]	[1,11]	2	dCas9-VP64	positive selection	2406153
32066083	32066106	2	-	25494202	A375	resistance to PLX-4720 (puromycin)	GCTACTCGGGAGACTGAGGCAAG	SPAST	ENSG00000021574	-0.7498462491276636	[113,13]	[10,3]	-4	dCas9-VP64	positive selection	2408554
32073308	32073331	2	+	25494202	A375	resistance to PLX-4720 (puromycin)	CAGGCGTGAGCCACTGCACCTGG	SPAST	ENSG00000021574	0.9550246130222104	[38,14]	[18,2]	4	dCas9-VP64	positive selection	2414130
32081689	32081712	2	+	25494202	A375	resistance to PLX-4720 (puromycin)	CAGGAAAATTGCTTGAACCCGAG	SPAST	ENSG00000021574	3.06787684374975	[49,8]	[78,27]	8	dCas9-VP64	positive selection	2415333
32079304	32079327	2	-	25494202	A375	resistance to PLX-4720 (puromycin)	CCAGGCTGGTCTTGAACTCCCGG	SPAST	ENSG00000021574	1.1112377139242877	[89,193]	[7,132]	4	dCas9-VP64	positive selection	2451686
32107478	32107501	2	-	25494202	A375	resistance to PLX-4720 (zeocin)	CAGGTGGATCACTTAAGGTCAGG	SPAST	ENSG00000021574	3.3442607452448634	[330,29]	[1241,17]	9	dCas9-VP64	positive selection	2484833
32068178	32068201	2	-	25494202	A375	resistance to PLX-4720 (zeocin)	CGAGACCATCCTGGCTAACACAG	SPAST	ENSG00000021574	0.5549091022422933	[154,188]	[77,85]	4	dCas9-VP64	positive selection	2499612
32146443	32146466	2	+	25494202	A375	resistance to PLX-4720 (zeocin)	CCCAGCTACTCGGGAGTCTGAAG	SPAST	ENSG00000021574	-0.014067359462650364	[104,52]	[22,26]	0	dCas9-VP64	positive selection	2501408
32066083	32066106	2	-	25494202	A375	resistance to PLX-4720 (zeocin)	GCTACTCGGGAGACTGAGGCAAG	SPAST	ENSG00000021574	-1.0734520873599889	[20,56]	[6,3]	-8	dCas9-VP64	positive selection	2503809
32073308	32073331	2	+	25494202	A375	resistance to PLX-4720 (zeocin)	CAGGCGTGAGCCACTGCACCTGG	SPAST	ENSG00000021574	-0.28279012529460834	[51,110]	[30,11]	-3	dCas9-VP64	positive selection	2509385
32081689	32081712	2	+	25494202	A375	resistance to PLX-4720 (zeocin)	CAGGAAAATTGCTTGAACCCGAG	SPAST	ENSG00000021574	0.014675341320169899	[37,72]	[27,8]	0	dCas9-VP64	positive selection	2510588
32079304	32079327	2	-	25494202	A375	resistance to PLX-4720 (zeocin)	CCAGGCTGGTCTTGAACTCCCGG	SPAST	ENSG00000021574	-0.05379639634842043	[67,143]	[20,43]	0	dCas9-VP64	positive selection	2546941
32064142	32064165	2	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCCCCGGCGCCGGTGCCGGGCGG	SPAST	ENSG00000021574	-0.21602643188709458	[2,2]	[0,2]	-3	hSpCas9	positive selection	3023012
32064083	32064106	2	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCGCCCTCATGGCAGCCAAGAGG	SPAST	ENSG00000021574	0.10571252482433843	[2,2]	[2,2]	2	hSpCas9	positive selection	3023013
32063834	32063857	2	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TGAATTCTCCGGGTGGACGAGGG	SPAST	ENSG00000021574	0.05989865352802602	[3,3]	[3,2]	1	hSpCas9	positive selection	3023014
32089589	32089612	2	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TGATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.18171258913878557	[4,5]	[5,4]	4	hSpCas9	positive selection	3023015
32064142	32064165	2	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCCCCGGCGCCGGTGCCGGGCGG	SPAST	ENSG00000021574	-0.18672959413886295	[2,2]	[2,0]	-3	hSpCas9	positive selection	3090870
32064083	32064106	2	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCGCCCTCATGGCAGCCAAGAGG	SPAST	ENSG00000021574	-0.3510669171251672	[2,2]	[1,0]	-5	hSpCas9	positive selection	3090871
32063834	32063857	2	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TGAATTCTCCGGGTGGACGAGGG	SPAST	ENSG00000021574	-0.21728155290787238	[3,3]	[3,0]	-3	hSpCas9	positive selection	3090872
32089589	32089612	2	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TGATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.010408095643014148	[4,4]	[4,2]	0	hSpCas9	positive selection	3090873
32087509	32087532		+	24336571	A375	resistance to PLX after 7 days	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.00906429460082503	[6,10]	[9,6]	0	hSpCas9	positive selection	3146914
32087551	32087574		+	24336571	A375	resistance to PLX after 7 days	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	-0.04879104781851229	[41,39]	[37,40]	-1	hSpCas9	positive selection	3146915
32114694	32114717		-	24336571	A375	resistance to PLX after 7 days	TTCATAACTGTTTTTGAACGAGG	SPAST	ENSG00000021574	-0.11780343723241427	[22,16]	[16,18]	-4	hSpCas9	positive selection	3146916
32087509	32087532		+	24336571	A375	resistance to PLX after 14 days	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.8983900225756796	[6,4]	[3,5]	7	hSpCas9	positive selection	3204707
32087551	32087574		+	24336571	A375	resistance to PLX after 14 days	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.9035038142403493	[50,36]	[33,42]	7	hSpCas9	positive selection	3204708
32114694	32114717		-	24336571	A375	resistance to PLX after 14 days	TTCATAACTGTTTTTGAACGAGG	SPAST	ENSG00000021574	-0.9164350692429872	[23,18]	[4,4]	-9	hSpCas9	positive selection	3204709
32087509	32087532		+	24336571	A375	viability after 7 days	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.05659883556772166	[8]	[6,10]	-1	hSpCas9	negative selection	3262500
32087551	32087574		+	24336571	A375	viability after 7 days	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.4498691446412473	[31]	[41,39]	6	hSpCas9	negative selection	3262501
32114694	32114717		-	24336571	A375	viability after 7 days	TTCATAACTGTTTTTGAACGAGG	SPAST	ENSG00000021574	0.5633248472889426	[13]	[22,16]	8	hSpCas9	negative selection	3262502
32087509	32087532		+	24336571	A375	viability after 14 days	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.5342141077798392	[8]	[6,4]	-6	hSpCas9	negative selection	3320293
32087551	32087574		+	24336571	A375	viability after 14 days	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.5506548842573575	[31]	[50,36]	7	hSpCas9	negative selection	3320294
32114694	32114717		-	24336571	A375	viability after 14 days	TTCATAACTGTTTTTGAACGAGG	SPAST	ENSG00000021574	0.6956328651325429	[13]	[23,18]	8	hSpCas9	negative selection	3320295
32115700	32115723	2	+	27383988	293T	resistance to West Nile virus (flavivirus)	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	1.0285075209499543	[74,74]	[0,0]	5	hSpCas9	positive selection	3394483
32087551	32087574	2	+	27383988	293T	resistance to West Nile virus (flavivirus)	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	-1.2305138223772163	[358,358]	[0,0]	-7	hSpCas9	positive selection	3394484
32087509	32087532	2	+	27383988	293T	resistance to West Nile virus (flavivirus)	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	2.672363710724679	[23,23]	[0,0]	8	hSpCas9	positive selection	3394485
32064141	32064164	2	+	26780180	HT29	viability (Avana library 4 designs)	GCCCCGGCGCCGGTGCCGGGCGG	SPAST	ENSG00000021574	0.551489880662828	[]	[]	2	hSpCas9	negative selection	3461635
32064082	32064105	2	+	26780180	HT29	viability (Avana library 4 designs)	GCGCCCTCATGGCAGCCAAGAGG	SPAST	ENSG00000021574	1.46546964953192	[]	[]	6	hSpCas9	negative selection	3461636
32063833	32063856	2	+	26780180	HT29	viability (Avana library 4 designs)	TGAATTCTCCGGGTGGACGAGGG	SPAST	ENSG00000021574	1.23977179337932	[]	[]	5	hSpCas9	negative selection	3461637
32089589	32089612	2	-	26780180	HT29	viability (Avana library 4 designs)	TGATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-0.630883984207153	[]	[]	-8	hSpCas9	negative selection	3461638
32089589	32089612	2	-	26780180	A375	viability (Avana lentiCRISPRv2)	TGATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-4.19461408584307	[]	[]	-5	hSpCas9	negative selection	3562600
32064082	32064105	2	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCGCCCTCATGGCAGCCAAGAGG	SPAST	ENSG00000021574	4.34990558750422	[]	[]	7	hSpCas9	negative selection	3562601
32064141	32064164	2	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCCCCGGCGCCGGTGCCGGGCGG	SPAST	ENSG00000021574	8.50803450120588	[]	[]	9	hSpCas9	negative selection	3562602
32063833	32063856	2	+	26780180	A375	viability (Avana lentiCRISPRv2)	TGAATTCTCCGGGTGGACGAGGG	SPAST	ENSG00000021574	2.0002722559459	[]	[]	3	hSpCas9	negative selection	3562603
32063996	32064019	2	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCGCGAAGCCTACAAACAGCGGG	SPAST	ENSG00000021574	-8.486979175182	[]	[]	-7	hSpCas9	negative selection	3562604
32064092	32064115	2	+	26780180	A375	viability (Avana lentiCRISPRv2)	GGCAGCCAAGAGGAGCTCCGGGG	SPAST	ENSG00000021574	-3.21865751205186	[]	[]	-4	hSpCas9	negative selection	3562605
32089589	32089612	2	-	26780180	A375	viability (Avana lentiGuide)	TGATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-2.15019120372904	[]	[]	-4	hSpCas9	negative selection	3671259
32064082	32064105	2	+	26780180	A375	viability (Avana lentiGuide)	GCGCCCTCATGGCAGCCAAGAGG	SPAST	ENSG00000021574	-4.7497017860364	[]	[]	-7	hSpCas9	negative selection	3671260
32064141	32064164	2	+	26780180	A375	viability (Avana lentiGuide)	GCCCCGGCGCCGGTGCCGGGCGG	SPAST	ENSG00000021574	3.80827699671476	[]	[]	4	hSpCas9	negative selection	3671261
32063833	32063856	2	+	26780180	A375	viability (Avana lentiGuide)	TGAATTCTCCGGGTGGACGAGGG	SPAST	ENSG00000021574	3.23189934496921	[]	[]	3	hSpCas9	negative selection	3671262
32063996	32064019	2	-	26780180	A375	viability (Avana lentiGuide)	GCGCGAAGCCTACAAACAGCGGG	SPAST	ENSG00000021574	13.3643509746218	[]	[]	7	hSpCas9	negative selection	3671263
32064092	32064115	2	+	26780180	A375	viability (Avana lentiGuide)	GGCAGCCAAGAGGAGCTCCGGGG	SPAST	ENSG00000021574	20.0842880042219	[]	[]	8	hSpCas9	negative selection	3671264
32089589	32089612	2	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-0.26605316121876504	[8]	[6,5]	-4	hSpCas9	positive selection	3779866
32064082	32064105	2	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCGCCCTCATGGCAGCCAAGAGG	SPAST	ENSG00000021574	0.34577588711017315	[4]	[5,5]	9	hSpCas9	positive selection	3779867
32064141	32064164	2	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCCCCGGCGCCGGTGCCGGGCGG	SPAST	ENSG00000021574	0.7497022611109092	[2]	[5,4]	9	hSpCas9	positive selection	3779868
32063833	32063856	2	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGAATTCTCCGGGTGGACGAGGG	SPAST	ENSG00000021574	0.07881622367270011	[5]	[5,5]	2	hSpCas9	positive selection	3779869
32063996	32064019	2	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCGCGAAGCCTACAAACAGCGGG	SPAST	ENSG00000021574	-0.4978437216591256	[3]	[2,1]	-6	hSpCas9	positive selection	3779870
32064092	32064115	2	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GGCAGCCAAGAGGAGCTCCGGGG	SPAST	ENSG00000021574	-0.4312746709709167	[3]	[3,1]	-6	hSpCas9	positive selection	3779871
32089589	32089612	2	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.26625871394797157	[6]	[7,6]	7	hSpCas9	positive selection	3888473
32064082	32064105	2	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCGCCCTCATGGCAGCCAAGAGG	SPAST	ENSG00000021574	0.05153093928716368	[4]	[3,3]	1	hSpCas9	positive selection	3888474
32064141	32064164	2	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCCCCGGCGCCGGTGCCGGGCGG	SPAST	ENSG00000021574	-0.6567834320876567	[4]	[1,2]	-7	hSpCas9	positive selection	3888475
32063833	32063856	2	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGAATTCTCCGGGTGGACGAGGG	SPAST	ENSG00000021574	-0.05222439265149406	[5]	[4,3]	0	hSpCas9	positive selection	3888476
32063996	32064019	2	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCGCGAAGCCTACAAACAGCGGG	SPAST	ENSG00000021574	-0.3473677413944974	[5]	[2,4]	-4	hSpCas9	positive selection	3888477
32064092	32064115	2	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GGCAGCCAAGAGGAGCTCCGGGG	SPAST	ENSG00000021574	-0.18496771604701467	[3]	[1,3]	-3	hSpCas9	positive selection	3888478
32115700	32115723	2	+	26780180	A375	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.79995466620932	[]	[]	3	hSpCas9	negative selection	3998516
32114744	32114767	2	-	26780180	A375	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.122041384943032	[]	[]	0	hSpCas9	negative selection	3998517
32114811	32114834	2	-	26780180	A375	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	1.14085812083931	[]	[]	6	hSpCas9	negative selection	3998518
32087509	32087532	2	+	26780180	A375	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.804955633200546	[]	[]	3	hSpCas9	negative selection	3998519
32087551	32087574	2	+	26780180	A375	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.81806774883408	[]	[]	3	hSpCas9	negative selection	3998520
32115741	32115764	2	-	26780180	A375	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	1.15219398252456	[]	[]	6	hSpCas9	negative selection	3998521
32115700	32115723	2	+	26780180	HT29	viability (GeCKOv2 library)	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.799939635657458	[]	[]	3	hSpCas9	negative selection	4106482
32114744	32114767	2	-	26780180	HT29	viability (GeCKOv2 library)	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.687773874058264	[]	[]	2	hSpCas9	negative selection	4106483
32114811	32114834	2	-	26780180	HT29	viability (GeCKOv2 library)	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.882466175956893	[]	[]	4	hSpCas9	negative selection	4106484
32087509	32087532	2	+	26780180	HT29	viability (GeCKOv2 library)	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.209257294642634	[]	[]	0	hSpCas9	negative selection	4106485
32087551	32087574	2	+	26780180	HT29	viability (GeCKOv2 library)	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.50737172560306	[]	[]	1	hSpCas9	negative selection	4106486
32115741	32115764	2	-	26780180	HT29	viability (GeCKOv2 library)	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.922342774640935	[]	[]	4	hSpCas9	negative selection	4106487
32087509	32087532	2	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.4432484016896715	[2]	[3,2]	8	hSpCas9	positive selection	4173054
32087551	32087574	2	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.3184957970683931	[5]	[4,5]	7	hSpCas9	positive selection	4173055
32114694	32114717	2	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TTCATAACTGTTTTTGAACGAGG	SPAST	ENSG00000021574	0.07716454700227546	[4]	[3,2]	2	hSpCas9	positive selection	4173056
32087509	32087532	2	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.12767005387531874	[3]	[1,3,1,0]	1	hSpCas9	positive selection	4237076
32087551	32087574	2	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.9896950820487802	[4]	[2,3,4,3]	7	hSpCas9	positive selection	4237077
32114694	32114717	2	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TTCATAACTGTTTTTGAACGAGG	SPAST	ENSG00000021574	0.992748413737097	[3]	[3,0,1,3]	7	hSpCas9	positive selection	4237078
32115741	32115764	2	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.12268949036516637	[2]	[3,1]	2	hSpCas9	positive selection	4300051
32114744	32114767	2	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.3458253593378937	[3]	[3,2]	7	hSpCas9	positive selection	4300052
32114811	32114834	2	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.2352239509422922	[1]	[1,0]	-5	hSpCas9	positive selection	4300053
32115700	32115723	2	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.2412679284143976	[1]	[1,0]	5	hSpCas9	positive selection	4364303
32087551	32087574	2	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	-0.1636084892510602	[3]	[3,0]	-3	hSpCas9	positive selection	4364304
32087509	32087532	2	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.5360963692472367	[1]	[1,1]	9	hSpCas9	positive selection	4364305
32063956	32063978	2	-	27760321	OCIAML3	viability	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	0.184562618701679	[696,584]	[537,605]	2	hSpCas9	negative selection	4442431
32063992	32064014	2	-	27760321	OCIAML3	viability	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.4427134658957125	[708,533]	[573,760]	5	hSpCas9	negative selection	4442432
32064216	32064238	2	+	27760321	OCIAML3	viability	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	-0.0034925592430724572	[865,719]	[510,752]	0	hSpCas9	negative selection	4442433
32089589	32089611	2	-	27760321	OCIAML3	viability	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-0.7116525288789415	[16,19]	[14,0]	-6	hSpCas9	negative selection	4442434
32115701	32115723	2	+	27760321	OCIAML3	viability	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.059524984909014256	[176,152]	[106,144]	0	hSpCas9	negative selection	4442435
32063956	32063978	2	-	27760321	OCIAML2	viability	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	0.156074658405277	[696,584]	[537,624]	2	hSpCas9	negative selection	4525894
32063992	32064014	2	-	27760321	OCIAML2	viability	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.0814207474609121	[708,533]	[445,634]	1	hSpCas9	negative selection	4525895
32064216	32064238	2	+	27760321	OCIAML2	viability	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	-2.4845234062154633	[865,719]	[114,112]	-9	hSpCas9	negative selection	4525896
32089589	32089611	2	-	27760321	OCIAML2	viability	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-0.4379031933704649	[16,19]	[8,13]	-5	hSpCas9	negative selection	4525897
32115701	32115723	2	+	27760321	OCIAML2	viability	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.0873113258401379	[176,152]	[101,155]	-1	hSpCas9	negative selection	4525898
32063956	32063978	2	-	27760321	MV411	viability	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	-0.6685738162533903	[696,584]	[366,111]	-4	hSpCas9	negative selection	4609357
32063992	32064014	2	-	27760321	MV411	viability	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.08266402627215441	[708,533]	[335,572]	0	hSpCas9	negative selection	4609358
32064216	32064238	2	+	27760321	MV411	viability	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	-0.3379557916038626	[865,719]	[468,326]	-2	hSpCas9	negative selection	4609359
32089589	32089611	2	-	27760321	MV411	viability	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.32156001366208997	[16,19]	[24,0]	2	hSpCas9	negative selection	4609360
32115701	32115723	2	+	27760321	MV411	viability	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.171956081864854	[176,152]	[83,114]	-1	hSpCas9	negative selection	4609361
32063956	32063978	2	-	27760321	HL60	viability	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	0.28596869342641595	[696,584]	[616,655]	3	hSpCas9	negative selection	4692820
32063992	32064014	2	-	27760321	HL60	viability	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-0.07024777369188645	[708,533]	[464,494]	-1	hSpCas9	negative selection	4692821
32064216	32064238	2	+	27760321	HL60	viability	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	0.49511101119882883	[865,719]	[779,1045]	6	hSpCas9	negative selection	4692822
32089589	32089611	2	-	27760321	HL60	viability	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	1.3457283130436521	[16,19]	[64,10]	9	hSpCas9	negative selection	4692823
32115701	32115723	2	+	27760321	HL60	viability	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.8663284907272879	[176,152]	[64,82]	-7	hSpCas9	negative selection	4692824
32063956	32063978	2	-	27760321	HT1080	viability	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	1.0700860542871315	[584,456]	[462,405]	6	hSpCas9	negative selection	4776283
32063992	32064014	2	-	27760321	HT1080	viability	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.1214298183226708	[533,416]	[80,342]	0	hSpCas9	negative selection	4776284
32064216	32064238	2	+	27760321	HT1080	viability	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	-1.9539615200951514	[719,561]	[63,67]	-6	hSpCas9	negative selection	4776285
32089589	32089611	2	-	27760321	HT1080	viability	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-2.844317238920959	[19,15]	[0,0]	-7	hSpCas9	negative selection	4776286
32115701	32115723	2	+	27760321	HT1080	viability	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.29838189075992405	[152,119]	[32,103]	1	hSpCas9	negative selection	4776287
32063956	32063978	2	-	27760321	HT29	viability after 7 days	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	-0.1617545191098963	[584,456]	[456,800,519]	-2	hSpCas9	negative selection	4859746
32063992	32064014	2	-	27760321	HT29	viability after 7 days	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.7758102489367384	[533,416]	[1065,979,1093]	9	hSpCas9	negative selection	4859747
32064216	32064238	2	+	27760321	HT29	viability after 7 days	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	-0.07582966032004057	[719,561]	[917,914,538]	-1	hSpCas9	negative selection	4859748
32089589	32089611	2	-	27760321	HT29	viability after 7 days	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-0.23744675697860895	[19,15]	[36,22,1]	-3	hSpCas9	negative selection	4859749
32115701	32115723	2	+	27760321	HT29	viability after 7 days	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.18356180905642483	[152,119]	[180,90,193]	-3	hSpCas9	negative selection	4859750
32063956	32063978	2	-	27760321	HT29	viability after 25 days	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	-0.12029251389248763	[584,456]	[607,489,539]	-1	hSpCas9	negative selection	4943209
32063992	32064014	2	-	27760321	HT29	viability after 25 days	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.7254192512321347	[533,416]	[864,684,1174]	8	hSpCas9	negative selection	4943210
32064216	32064238	2	+	27760321	HT29	viability after 25 days	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	0.6419191729838579	[719,561]	[1193,1244,966]	7	hSpCas9	negative selection	4943211
32089589	32089611	2	-	27760321	HT29	viability after 25 days	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-1.1525978548868165	[19,15]	[23,0,0]	-7	hSpCas9	negative selection	4943212
32115701	32115723	2	+	27760321	HT29	viability after 25 days	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.21695771724697632	[152,119]	[125,233,187]	2	hSpCas9	negative selection	4943213
32063956	32063978	2	-	27760321	HT29	viability after 22 days	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	0.27501145411249384	[584,456]	[868,654,657]	3	hSpCas9	negative selection	5026672
32063992	32064014	2	-	27760321	HT29	viability after 22 days	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.5715376121439284	[533,416]	[712,986,752]	6	hSpCas9	negative selection	5026673
32064216	32064238	2	+	27760321	HT29	viability after 22 days	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	0.5178202293631561	[719,561]	[816,1265,1106]	6	hSpCas9	negative selection	5026674
32089589	32089611	2	-	27760321	HT29	viability after 22 days	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-0.703712304923636	[19,15]	[22,13,0]	-5	hSpCas9	negative selection	5026675
32115701	32115723	2	+	27760321	HT29	viability after 22 days	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.1938267359650805	[152,119]	[57,237,119]	-2	hSpCas9	negative selection	5026676
32063956	32063978	2	-	27760321	HT29	viability after 19 days	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	-0.0626944498223807	[584,456]	[339,665,676]	0	hSpCas9	negative selection	5110135
32063992	32064014	2	-	27760321	HT29	viability after 19 days	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.4900771944567542	[533,416]	[931,650,726]	5	hSpCas9	negative selection	5110136
32064216	32064238	2	+	27760321	HT29	viability after 19 days	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	-0.02190844028969463	[719,561]	[960,542,687]	0	hSpCas9	negative selection	5110137
32089589	32089611	2	-	27760321	HT29	viability after 19 days	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.11738341195737245	[19,15]	[70,0,0]	1	hSpCas9	negative selection	5110138
32115701	32115723	2	+	27760321	HT29	viability after 19 days	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.3953180360920283	[152,119]	[198,281,136]	4	hSpCas9	negative selection	5110139
32063956	32063978	2	-	27760321	HT29	viability after 16 days	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	-0.2063285430449237	[584,456]	[397,295,816]	-2	hSpCas9	negative selection	5193598
32063992	32064014	2	-	27760321	HT29	viability after 16 days	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.7852402313156728	[533,416]	[944,689,1123]	8	hSpCas9	negative selection	5193599
32064216	32064238	2	+	27760321	HT29	viability after 16 days	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	0.6181047378535215	[719,561]	[1182,1054,1072]	7	hSpCas9	negative selection	5193600
32089589	32089611	2	-	27760321	HT29	viability after 16 days	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.2609009635738406	[19,15]	[75,0,0]	3	hSpCas9	negative selection	5193601
32115701	32115723	2	+	27760321	HT29	viability after 16 days	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.8343734218841184	[152,119]	[228,349,231]	8	hSpCas9	negative selection	5193602
32063956	32063978	2	-	27760321	HT29	viability after 13 days	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	-0.09107363756577419	[584,456]	[653,546,504]	-1	hSpCas9	negative selection	5277061
32063992	32064014	2	-	27760321	HT29	viability after 13 days	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.7655411165221171	[533,416]	[1212,821,786]	8	hSpCas9	negative selection	5277062
32064216	32064238	2	+	27760321	HT29	viability after 13 days	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	0.039297314365796976	[719,561]	[510,762,1003]	0	hSpCas9	negative selection	5277063
32089589	32089611	2	-	27760321	HT29	viability after 13 days	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.09509336104983035	[19,15]	[61,0,4]	1	hSpCas9	negative selection	5277064
32115701	32115723	2	+	27760321	HT29	viability after 13 days	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.15741669992588014	[152,119]	[98,234,194]	2	hSpCas9	negative selection	5277065
32063956	32063978	2	-	27760321	HT29	viability after 10 days	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	-0.29619199075467617	[584,456]	[425,449,706]	-4	hSpCas9	negative selection	5360524
32063992	32064014	2	-	27760321	HT29	viability after 10 days	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.8196236275065509	[533,416]	[884,931,1316]	9	hSpCas9	negative selection	5360525
32064216	32064238	2	+	27760321	HT29	viability after 10 days	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	0.2021858999836229	[719,561]	[640,1024,1094]	2	hSpCas9	negative selection	5360526
32089589	32089611	2	-	27760321	HT29	viability after 10 days	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	-2.93302357450555	[19,15]	[0,6,0]	-9	hSpCas9	negative selection	5360527
32115701	32115723	2	+	27760321	HT29	viability after 10 days	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.3575913503970227	[152,119]	[245,280,131]	5	hSpCas9	negative selection	5360528
32063956	32063978	2	-	27760321	MOLM13	viability	TTCCGCTTATGCGGCGACTCGG	SPAST	ENSG00000021574	1.6009257110060173	[696,584]	[779,1358]	9	hSpCas9	negative selection	5443987
32063992	32064014	2	-	27760321	MOLM13	viability	AAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.36039243958437805	[708,533]	[554,353]	2	hSpCas9	negative selection	5443988
32064216	32064238	2	+	27760321	MOLM13	viability	ATTGCCCTGCGCATCGATGAGG	SPAST	ENSG00000021574	0.6555096239955054	[865,719]	[664,732]	4	hSpCas9	negative selection	5443989
32089589	32089611	2	-	27760321	MOLM13	viability	GATACCTAGAAGTTGTAAGCGG	SPAST	ENSG00000021574	0.9933642901180146	[16,19]	[0,37]	7	hSpCas9	negative selection	5443990
32115701	32115723	2	+	27760321	MOLM13	viability	GGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-1.0142787154459403	[176,152]	[32,56]	-6	hSpCas9	negative selection	5443991
32063766	32063789	2	-	27661255	K562	viability	GACCGACGGGAACCAAGCGACGG	SPAST	ENSG00000021574	-0.0930058250504695	[539,526]	[443,361]	-3	dCas9-KRAB	negative selection	5513730
32063978	32064001	2	-	27661255	K562	viability	GCGGGTAGGAGAAATAGTACAGG	SPAST	ENSG00000021574	-0.22636515177007954	[1582,1572]	[1231,945]	-6	dCas9-KRAB	negative selection	5513731
32063992	32064015	2	-	27661255	K562	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-0.09976324961648092	[613,701]	[545,440]	-4	dCas9-KRAB	negative selection	5513732
32115741	32115764	2	-	27260156	BXPC3	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.11199521785250355	[341]	[300,306,353,368]	-3	hSpCas9	negative selection	5572511
32114744	32114767	2	-	27260156	BXPC3	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.0725289778120854	[561]	[522,569,749,642]	1	hSpCas9	negative selection	5572512
32114811	32114834	2	-	27260156	BXPC3	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.41375325464447943	[186]	[207,197,308,345]	8	hSpCas9	negative selection	5572513
32115700	32115723	2	+	27260156	BXPC3	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.3598650487829613	[85]	[106,63,153,143]	7	hSpCas9	negative selection	5636763
32087551	32087574	2	+	27260156	BXPC3	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.329340791656328	[578]	[615,651,857,959]	7	hSpCas9	negative selection	5636764
32087509	32087532	2	+	27260156	BXPC3	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.5434852679515321	[68]	[63,87,140,136]	9	hSpCas9	negative selection	5636765
32115741	32115764	2	-	27260156	A673	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.003542876984388932	[341]	[356,405,404,466]	0	hSpCas9	negative selection	5693762
32114744	32114767	2	-	27260156	A673	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	-0.054767923874729285	[561]	[663,532,741,662]	-1	hSpCas9	negative selection	5693763
32114811	32114834	2	-	27260156	A673	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.25279100185985026	[186]	[297,323,188,260]	6	hSpCas9	negative selection	5693764
32115700	32115723	2	+	27260156	A673	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.27676666568230734	[85]	[148,138,116,95]	6	hSpCas9	negative selection	5758014
32087551	32087574	2	+	27260156	A673	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.2040884095409684	[578]	[755,847,783,807]	5	hSpCas9	negative selection	5758015
32087509	32087532	2	+	27260156	A673	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.2334851382329517	[68]	[117,102,56,114]	5	hSpCas9	negative selection	5758016
32115741	32115764	2	-	27260156	A375	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.12715554432871667	[341]	[440,546,539,252]	1	hSpCas9	negative selection	5815013
32114744	32114767	2	-	27260156	A375	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.45261217246073704	[561]	[648,1273,909,816]	6	hSpCas9	negative selection	5815014
32114811	32114834	2	-	27260156	A375	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.20019697758986088	[186]	[191,253,215,330]	3	hSpCas9	negative selection	5815015
32115700	32115723	2	+	27260156	A375	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.2611471567078989	[85]	[84,255,96,67]	4	hSpCas9	negative selection	5879265
32087551	32087574	2	+	27260156	A375	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.40967225330326795	[578]	[791,1290,803,771]	6	hSpCas9	negative selection	5879266
32087509	32087532	2	+	27260156	A375	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.26059110349047465	[68]	[106,173,86,35]	4	hSpCas9	negative selection	5879267
32115741	32115764	2	-	27260156	COLO741	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.09536724320118384	[341]	[552,316,370]	-2	hSpCas9	negative selection	5936264
32114744	32114767	2	-	27260156	COLO741	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.29547624036264253	[561]	[826,895,911]	6	hSpCas9	negative selection	5936265
32114811	32114834	2	-	27260156	COLO741	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.3684398787401415	[186]	[226,299,384]	7	hSpCas9	negative selection	5936266
32115700	32115723	2	+	27260156	COLO741	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.3460527103525597	[85]	[203,107,113]	7	hSpCas9	negative selection	6000516
32087551	32087574	2	+	27260156	COLO741	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.19359080297448672	[578]	[843,775,910]	4	hSpCas9	negative selection	6000517
32087509	32087532	2	+	27260156	COLO741	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.8110939563260411	[68]	[140,144,174]	9	hSpCas9	negative selection	6000518
32115741	32115764	2	-	27260156	CAL120	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.1400080832381429	[341]	[596,419,409]	2	hSpCas9	negative selection	6057515
32114744	32114767	2	-	27260156	CAL120	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.5717345762894451	[561]	[1828,608,802]	8	hSpCas9	negative selection	6057516
32114811	32114834	2	-	27260156	CAL120	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.0998128943274319	[186]	[184,159,299]	-1	hSpCas9	negative selection	6057517
32115700	32115723	2	+	27260156	CAL120	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.42569130489518514	[85]	[129,148,149]	6	hSpCas9	negative selection	6121767
32087551	32087574	2	+	27260156	CAL120	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.5522399373514364	[578]	[1034,1081,1048]	7	hSpCas9	negative selection	6121768
32087509	32087532	2	+	27260156	CAL120	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.5720008028144279	[68]	[63,164,143]	8	hSpCas9	negative selection	6121769
32115741	32115764	2	-	27260156	CADOES1	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.2702819558905912	[341]	[364,440,386,339]	4	hSpCas9	negative selection	6178766
32114744	32114767	2	-	27260156	CADOES1	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.21935945461835843	[561]	[535,734,594,566]	4	hSpCas9	negative selection	6178767
32114811	32114834	2	-	27260156	CADOES1	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.25645394316526016	[186]	[299,214,179,132]	4	hSpCas9	negative selection	6178768
32115700	32115723	2	+	27260156	CADOES1	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	1.0988495475534263	[85]	[185,201,158,136]	9	hSpCas9	negative selection	6243018
32087551	32087574	2	+	27260156	CADOES1	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.5622594589521877	[578]	[607,908,875,787]	8	hSpCas9	negative selection	6243019
32087509	32087532	2	+	27260156	CADOES1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.21838058913383263	[68]	[81,57,98,58]	3	hSpCas9	negative selection	6243020
32115741	32115764	2	-	27260156	EWS502	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.11334463733566957	[341]	[218,390,388,352]	-2	hSpCas9	negative selection	6300017
32114744	32114767	2	-	27260156	EWS502	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.2966156104343637	[561]	[478,694,847,948]	5	hSpCas9	negative selection	6300018
32114811	32114834	2	-	27260156	EWS502	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.03507814484838656	[186]	[132,294,239,143]	0	hSpCas9	negative selection	6300019
32115700	32115723	2	+	27260156	EWS502	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.5139410272050751	[85]	[89,74,141,226]	8	hSpCas9	negative selection	6364269
32087551	32087574	2	+	27260156	EWS502	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.288394943803409	[578]	[614,765,885,742]	5	hSpCas9	negative selection	6364270
32087509	32087532	2	+	27260156	EWS502	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.11145743007971864	[68]	[36,71,143,67]	2	hSpCas9	negative selection	6364271
32115741	32115764	2	-	27260156	EW8	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.30548921802615203	[341]	[352,201,198,160]	-5	hSpCas9	negative selection	6421268
32114744	32114767	2	-	27260156	EW8	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.08431735919346373	[561]	[518,425,426,570]	1	hSpCas9	negative selection	6421269
32114811	32114834	2	-	27260156	EW8	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.08593185496303812	[186]	[209,145,174,114]	1	hSpCas9	negative selection	6421270
32115700	32115723	2	+	27260156	EW8	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.5689410691367007	[85]	[104,167,78,65]	8	hSpCas9	negative selection	6485520
32087551	32087574	2	+	27260156	EW8	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.223566875294966	[578]	[556,534,432,684]	4	hSpCas9	negative selection	6485521
32087509	32087532	2	+	27260156	EW8	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.38368364146005396	[68]	[100,59,58,76]	7	hSpCas9	negative selection	6485522
32115741	32115764	2	-	27260156	CORL105	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.13756976422500222	[341]	[419,568,472,301]	2	hSpCas9	negative selection	6542519
32114744	32114767	2	-	27260156	CORL105	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.34401864025925155	[561]	[922,635,1037,781]	6	hSpCas9	negative selection	6542520
32114811	32114834	2	-	27260156	CORL105	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.2818611306039309	[186]	[220,280,293,270]	5	hSpCas9	negative selection	6542521
32115700	32115723	2	+	27260156	CORL105	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.4700763400579486	[85]	[136,111,194,122]	8	hSpCas9	negative selection	6606771
32087551	32087574	2	+	27260156	CORL105	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.23734737429686115	[578]	[827,731,923,733]	4	hSpCas9	negative selection	6606772
32087509	32087532	2	+	27260156	CORL105	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.2480693466585504	[68]	[113,82,85,99]	4	hSpCas9	negative selection	6606773
32115741	32115764	2	-	27260156	HS294T	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.04058956727735247	[341]	[106,108,181,151]	0	hSpCas9	negative selection	6663770
32114744	32114767	2	-	27260156	HS294T	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.5451981301579926	[561]	[225,272,374,420]	7	hSpCas9	negative selection	6663771
32114811	32114834	2	-	27260156	HS294T	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.7721405951908435	[186]	[157,129,102,93]	8	hSpCas9	negative selection	6663772
32115700	32115723	2	+	27260156	HS294T	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	1.1898961683589397	[85]	[40,165,47,42]	9	hSpCas9	negative selection	6728022
32087551	32087574	2	+	27260156	HS294T	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.5541084072604247	[578]	[203,417,310,401]	7	hSpCas9	negative selection	6728023
32087509	32087532	2	+	27260156	HS294T	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.054416249638939984	[68]	[25,16,13,49]	0	hSpCas9	negative selection	6728024
32115741	32115764	2	-	27260156	HCC44	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.18824132876213773	[341]	[334,294,467,286]	-3	hSpCas9	negative selection	6785021
32114744	32114767	2	-	27260156	HCC44	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.24420177357006234	[561]	[932,768,845,495]	4	hSpCas9	negative selection	6785022
32114811	32114834	2	-	27260156	HCC44	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.4461448579448889	[186]	[225,314,408,213]	7	hSpCas9	negative selection	6785023
32115700	32115723	2	+	27260156	HCC44	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	1.0366885572817117	[85]	[283,169,179,171]	9	hSpCas9	negative selection	6849273
32087551	32087574	2	+	27260156	HCC44	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.40799782673346663	[578]	[768,719,932,1062]	6	hSpCas9	negative selection	6849274
32087509	32087532	2	+	27260156	HCC44	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.24565211382255958	[68]	[144,19,69,42]	-4	hSpCas9	negative selection	6849275
32115741	32115764	2	-	27260156	G402	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.2725493608148343	[341]	[781,385,488,395]	3	hSpCas9	negative selection	6906272
32114744	32114767	2	-	27260156	G402	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	-0.012504984377015915	[561]	[1081,909,536,278]	0	hSpCas9	negative selection	6906273
32114811	32114834	2	-	27260156	G402	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.17506594692115818	[186]	[391,249,54,346]	2	hSpCas9	negative selection	6906274
32115700	32115723	2	+	27260156	G402	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.3467887231531668	[85]	[122,57,45,105]	-4	hSpCas9	negative selection	6970524
32087551	32087574	2	+	27260156	G402	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.47143947194797275	[578]	[1013,1081,969,864]	6	hSpCas9	negative selection	6970525
32087509	32087532	2	+	27260156	G402	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.17692186970946544	[68]	[72,19,252,29]	2	hSpCas9	negative selection	6970526
32115741	32115764	2	-	27260156	L33	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.2514971705038108	[341]	[242,125,202,276]	-5	hSpCas9	negative selection	7027523
32114744	32114767	2	-	27260156	L33	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.1741857001245763	[561]	[675,272,398,558]	4	hSpCas9	negative selection	7027524
32114811	32114834	2	-	27260156	L33	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.3582436869022658	[186]	[207,85,141,314]	7	hSpCas9	negative selection	7027525
32115700	32115723	2	+	27260156	L33	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.18057483276426778	[85]	[72,39,68,109]	4	hSpCas9	negative selection	7091775
32087551	32087574	2	+	27260156	L33	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.3854370441618641	[578]	[846,323,389,763]	7	hSpCas9	negative selection	7091776
32087509	32087532	2	+	27260156	L33	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.8232553538511433	[68]	[118,51,69,130]	9	hSpCas9	negative selection	7091777
32115741	32115764	2	-	27260156	K562	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.1855350333071365	[341]	[210,356,287,494]	2	hSpCas9	negative selection	7148774
32114744	32114767	2	-	27260156	K562	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.02913632263146193	[561]	[617,395,678,360]	0	hSpCas9	negative selection	7148775
32114811	32114834	2	-	27260156	K562	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.4906114404320243	[186]	[75,260,86,58]	-5	hSpCas9	negative selection	7148776
32115700	32115723	2	+	27260156	K562	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.6326064238165676	[85]	[143,83,125,118]	7	hSpCas9	negative selection	7213026
32087551	32087574	2	+	27260156	K562	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.47861467738319474	[578]	[780,929,748,448]	6	hSpCas9	negative selection	7213027
32087509	32087532	2	+	27260156	K562	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.28072391398653007	[68]	[25,125,116,31]	3	hSpCas9	negative selection	7213028
32115741	32115764	2	-	27260156	HT29	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.007815765017607834	[341]	[371,607,257,424]	0	hSpCas9	negative selection	7270025
32114744	32114767	2	-	27260156	HT29	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.09871401102839245	[561]	[802,619,790,705]	2	hSpCas9	negative selection	7270026
32114811	32114834	2	-	27260156	HT29	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.2084905903533394	[186]	[262,182,315,277]	4	hSpCas9	negative selection	7270027
32115700	32115723	2	+	27260156	HT29	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.3701226731552705	[85]	[144,70,167,148]	7	hSpCas9	negative selection	7334277
32087551	32087574	2	+	27260156	HT29	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.4303985646726909	[578]	[1086,859,1081,781]	8	hSpCas9	negative selection	7334278
32087509	32087532	2	+	27260156	HT29	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.23840924034202393	[68]	[108,168,88,41]	4	hSpCas9	negative selection	7334279
32115741	32115764	2	-	27260156	MHHES1	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.3992328231787775	[341]	[445,577,575,436]	7	hSpCas9	negative selection	7391276
32114744	32114767	2	-	27260156	MHHES1	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.27994285866613644	[561]	[998,745,797,582]	5	hSpCas9	negative selection	7391277
32114811	32114834	2	-	27260156	MHHES1	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.530504707859183	[186]	[321,532,202,200]	8	hSpCas9	negative selection	7391278
32115700	32115723	2	+	27260156	MHHES1	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.43645500303789486	[85]	[132,101,128,149]	7	hSpCas9	negative selection	7455528
32087551	32087574	2	+	27260156	MHHES1	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.378973400777849	[578]	[899,914,961,654]	6	hSpCas9	negative selection	7455529
32087509	32087532	2	+	27260156	MHHES1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.02156653885380244	[68]	[62,22,100,103]	0	hSpCas9	negative selection	7455530
32115741	32115764	2	-	27260156	MEWO	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.3825194036433869	[341]	[362,254,140]	-7	hSpCas9	negative selection	7512527
32114744	32114767	2	-	27260156	MEWO	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	-0.14012037071078215	[561]	[405,574,435]	-3	hSpCas9	negative selection	7512528
32114811	32114834	2	-	27260156	MEWO	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.021230246070849113	[186]	[176,179,160]	0	hSpCas9	negative selection	7512529
32115700	32115723	2	+	27260156	MEWO	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.4566304596699604	[85]	[93,118,114]	8	hSpCas9	negative selection	7576779
32087551	32087574	2	+	27260156	MEWO	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	-0.08690524614070802	[578]	[668,459,431]	-2	hSpCas9	negative selection	7576780
32087509	32087532	2	+	27260156	MEWO	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	1.0133720812285558	[68]	[131,176,81]	9	hSpCas9	negative selection	7576781
32115741	32115764	2	-	27260156	LNCAPCLONEFGC	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.09356053729691449	[341]	[219,294,355,300]	-1	hSpCas9	negative selection	7633778
32114744	32114767	2	-	27260156	LNCAPCLONEFGC	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.19171759990513432	[561]	[526,619,716,479]	3	hSpCas9	negative selection	7633779
32114811	32114834	2	-	27260156	LNCAPCLONEFGC	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.3630904101401624	[186]	[180,250,238,201]	6	hSpCas9	negative selection	7633780
32115700	32115723	2	+	27260156	LNCAPCLONEFGC	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.6573489987689531	[85]	[94,86,167,150]	9	hSpCas9	negative selection	7698030
32087551	32087574	2	+	27260156	LNCAPCLONEFGC	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.48747649393706094	[578]	[957,564,736,687]	7	hSpCas9	negative selection	7698031
32087509	32087532	2	+	27260156	LNCAPCLONEFGC	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.7642884358329192	[68]	[169,67,133,57]	9	hSpCas9	negative selection	7698032
32115741	32115764	2	-	27260156	PANC0327	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.5374060509205663	[341]	[548,436,440,345]	7	hSpCas9	negative selection	7755029
32114744	32114767	2	-	27260156	PANC0327	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.5589520116612016	[561]	[564,920,845,571]	7	hSpCas9	negative selection	7755030
32114811	32114834	2	-	27260156	PANC0327	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.7028088549685911	[186]	[175,65,131,44]	-7	hSpCas9	negative selection	7755031
32115700	32115723	2	+	27260156	PANC0327	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.34458869597213626	[85]	[21,33,127,56]	-5	hSpCas9	negative selection	7819281
32087551	32087574	2	+	27260156	PANC0327	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.2943344518341545	[578]	[487,523,778,733]	4	hSpCas9	negative selection	7819282
32087509	32087532	2	+	27260156	PANC0327	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	1.0025411773522934	[68]	[196,162,61,69]	9	hSpCas9	negative selection	7819283
32115741	32115764	2	-	27260156	NCIH2009	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.22711565453224689	[341]	[459,520,676]	3	hSpCas9	negative selection	7876280
32114744	32114767	2	-	27260156	NCIH2009	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.3992397771179884	[561]	[1227,808,1109]	5	hSpCas9	negative selection	7876281
32114811	32114834	2	-	27260156	NCIH2009	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.7142527064389709	[186]	[350,420,497]	8	hSpCas9	negative selection	7876282
32115700	32115723	2	+	27260156	NCIH2009	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	1.2041499384429797	[85]	[247,240,335]	9	hSpCas9	negative selection	7940532
32087551	32087574	2	+	27260156	NCIH2009	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.20601969857594038	[578]	[1256,812,793]	3	hSpCas9	negative selection	7940533
32087509	32087532	2	+	27260156	NCIH2009	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.1449269112988869	[68]	[219,51,70]	2	hSpCas9	negative selection	7940534
32115741	32115764	2	-	27260156	NCIH1373	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.9759248054705212	[341]	[718,397,1179,193]	8	hSpCas9	negative selection	7997531
32114744	32114767	2	-	27260156	NCIH1373	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.24851331342495053	[561]	[800,712,618,230]	2	hSpCas9	negative selection	7997532
32114811	32114834	2	-	27260156	NCIH1373	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.011928004847243878	[186]	[283,157,104,84]	0	hSpCas9	negative selection	7997533
32115700	32115723	2	+	27260156	NCIH1373	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	1.7009840935758125	[85]	[125,686,264,23]	9	hSpCas9	negative selection	8061783
32087551	32087574	2	+	27260156	NCIH1373	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.6713093226815962	[578]	[1057,932,670,398]	6	hSpCas9	negative selection	8061784
32087509	32087532	2	+	27260156	NCIH1373	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.1096635398286483	[68]	[170,34,31,17]	-1	hSpCas9	negative selection	8061785
32115741	32115764	2	-	27260156	PATU8902	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.20981260944027738	[341]	[406,736,711,269]	2	hSpCas9	negative selection	8118782
32114744	32114767	2	-	27260156	PATU8902	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.8119516392890899	[561]	[1852,991,1350,1275]	8	hSpCas9	negative selection	8118783
32114811	32114834	2	-	27260156	PATU8902	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.5634837635994451	[186]	[236,143,331,899]	6	hSpCas9	negative selection	8118784
32115700	32115723	2	+	27260156	PATU8902	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.9676248160415755	[85]	[140,179,224,413]	9	hSpCas9	negative selection	8183034
32087551	32087574	2	+	27260156	PATU8902	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.47550447486288105	[578]	[1705,1161,526,1241]	5	hSpCas9	negative selection	8183035
32087509	32087532	2	+	27260156	PATU8902	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.415004650395161	[68]	[81,53,132,256]	4	hSpCas9	negative selection	8183036
32115741	32115764	2	-	27260156	PANC1	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.28790105586568804	[341]	[258,477,549,196]	4	hSpCas9	negative selection	8240033
32114744	32114767	2	-	27260156	PANC1	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.015813134397239803	[561]	[432,416,590,489]	0	hSpCas9	negative selection	8240034
32114811	32114834	2	-	27260156	PANC1	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.022209884560794313	[186]	[185,172,140,135]	0	hSpCas9	negative selection	8240035
32115700	32115723	2	+	27260156	PANC1	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.5608030765414085	[85]	[131,128,58,115]	8	hSpCas9	negative selection	8304285
32087551	32087574	2	+	27260156	PANC1	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.37301705789601824	[578]	[705,754,662,488]	6	hSpCas9	negative selection	8304286
32087509	32087532	2	+	27260156	PANC1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	1.015318426616205	[68]	[116,262,73,61]	9	hSpCas9	negative selection	8304287
32115741	32115764	2	-	27260156	PANC0813	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.17036481720948993	[341]	[568,297,233,345]	-3	hSpCas9	negative selection	8361284
32114744	32114767	2	-	27260156	PANC0813	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.47867678002144887	[561]	[811,1098,912,847]	7	hSpCas9	negative selection	8361285
32114811	32114834	2	-	27260156	PANC0813	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.34381199572450694	[186]	[306,210,346,253]	5	hSpCas9	negative selection	8361286
32115700	32115723	2	+	27260156	PANC0813	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.8923352041991468	[85]	[245,228,183,100]	9	hSpCas9	negative selection	8425536
32087551	32087574	2	+	27260156	PANC0813	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.21316414465882105	[578]	[756,616,1030,747]	3	hSpCas9	negative selection	8425537
32087509	32087532	2	+	27260156	PANC0813	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.2124113665620211	[68]	[130,132,45,69]	3	hSpCas9	negative selection	8425538
32115741	32115764	2	-	27260156	RDES	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.23338362708620655	[341]	[358,403,512,612]	4	hSpCas9	negative selection	8482535
32114744	32114767	2	-	27260156	RDES	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.2683049913887853	[561]	[547,896,837,860]	4	hSpCas9	negative selection	8482536
32114811	32114834	2	-	27260156	RDES	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.2474780922700328	[186]	[264,216,289,240]	4	hSpCas9	negative selection	8482537
32115700	32115723	2	+	27260156	RDES	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.16696757922963146	[85]	[75,127,100,146]	2	hSpCas9	negative selection	8546787
32087551	32087574	2	+	27260156	RDES	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.448163927579451	[578]	[659,1028,1007,963]	7	hSpCas9	negative selection	8546788
32087509	32087532	2	+	27260156	RDES	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.44882212366731933	[68]	[123,44,149,115]	7	hSpCas9	negative selection	8546789
32115741	32115764	2	-	27260156	PC3	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.19783099584653074	[341]	[152,354,516,283]	-4	hSpCas9	negative selection	8603786
32114744	32114767	2	-	27260156	PC3	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	-0.08480580627484338	[561]	[366,511,827,537]	-2	hSpCas9	negative selection	8603787
32114811	32114834	2	-	27260156	PC3	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.48581168126400587	[186]	[92,356,375,316]	8	hSpCas9	negative selection	8603788
32115700	32115723	2	+	27260156	PC3	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.08848989471466284	[85]	[54,78,111,91]	-2	hSpCas9	negative selection	8668038
32087551	32087574	2	+	27260156	PC3	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.08176645794514792	[578]	[314,593,874,852]	1	hSpCas9	negative selection	8668039
32087509	32087532	2	+	27260156	PC3	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.7085088016327381	[68]	[139,16,269,54]	9	hSpCas9	negative selection	8668040
32115741	32115764	2	-	27260156	PATU8988T	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.19151864830959142	[341]	[390,406,364,346]	3	hSpCas9	negative selection	8725037
32114744	32114767	2	-	27260156	PATU8988T	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.07940856560395099	[561]	[518,702,521,562]	1	hSpCas9	negative selection	8725038
32114811	32114834	2	-	27260156	PATU8988T	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.5699409672620551	[186]	[164,362,270,283]	8	hSpCas9	negative selection	8725039
32115700	32115723	2	+	27260156	PATU8988T	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.7282749742425219	[85]	[118,160,133,138]	9	hSpCas9	negative selection	8789289
32087551	32087574	2	+	27260156	PATU8988T	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.6053010816762167	[578]	[747,707,1039,895]	8	hSpCas9	negative selection	8789290
32087509	32087532	2	+	27260156	PATU8988T	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.4165452990113505	[68]	[54,28,30,85]	-6	hSpCas9	negative selection	8789291
32115741	32115764	2	-	27260156	T47D	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	-0.020822514349358723	[341]	[442,319,437,374]	0	hSpCas9	negative selection	8846288
32114744	32114767	2	-	27260156	T47D	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.0537125444967142	[561]	[768,549,686,724]	1	hSpCas9	negative selection	8846289
32114811	32114834	2	-	27260156	T47D	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.17327297560968402	[186]	[197,274,230,276]	3	hSpCas9	negative selection	8846290
32115700	32115723	2	+	27260156	T47D	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.21787645921790944	[85]	[124,112,100,127]	4	hSpCas9	negative selection	8910540
32087551	32087574	2	+	27260156	T47D	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.3861463668458842	[578]	[887,844,782,1018]	7	hSpCas9	negative selection	8910541
32087509	32087532	2	+	27260156	T47D	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.6831921528632523	[68]	[152,110,83,171]	9	hSpCas9	negative selection	8910542
32115741	32115764	2	-	27260156	SU8686	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.06504694839840008	[341]	[304,273,418,451]	1	hSpCas9	negative selection	8967539
32114744	32114767	2	-	27260156	SU8686	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.3524643785495205	[561]	[610,781,739,709]	6	hSpCas9	negative selection	8967540
32114811	32114834	2	-	27260156	SU8686	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.3356813718315036	[186]	[226,227,122,377]	5	hSpCas9	negative selection	8967541
32115700	32115723	2	+	27260156	SU8686	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.2911769018780146	[85]	[112,72,125,108]	5	hSpCas9	negative selection	9031791
32087551	32087574	2	+	27260156	SU8686	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.49500735573606014	[578]	[658,775,845,1002]	8	hSpCas9	negative selection	9031792
32087509	32087532	2	+	27260156	SU8686	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.5709521585849378	[68]	[85,74,101,156]	8	hSpCas9	negative selection	9031793
32115741	32115764	2	-	27260156	SKES1	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.07484933924958637	[341]	[315,414,329,371]	1	hSpCas9	negative selection	9088790
32114744	32114767	2	-	27260156	SKES1	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.1294306899285077	[561]	[721,609,533,597]	3	hSpCas9	negative selection	9088791
32114811	32114834	2	-	27260156	SKES1	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.029186762637986696	[186]	[265,207,138,162]	0	hSpCas9	negative selection	9088792
32115700	32115723	2	+	27260156	SKES1	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	-0.43329345193939783	[85]	[42,43,88,70]	-7	hSpCas9	negative selection	9153042
32087551	32087574	2	+	27260156	SKES1	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.29727851380647363	[578]	[784,771,729,577]	6	hSpCas9	negative selection	9153043
32087509	32087532	2	+	27260156	SKES1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.4551773074100378	[68]	[136,137,43,71]	8	hSpCas9	negative selection	9153044
32115741	32115764	2	-	27260156	TOV112D	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.18658920558167685	[341]	[343,327,456,409]	3	hSpCas9	negative selection	9210041
32114744	32114767	2	-	27260156	TOV112D	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	-0.1439488378923947	[561]	[422,577,483,559]	-3	hSpCas9	negative selection	9210042
32114811	32114834	2	-	27260156	TOV112D	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.2736234351617721	[186]	[86,323,184,61]	-5	hSpCas9	negative selection	9210043
32115700	32115723	2	+	27260156	TOV112D	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.4460433312929153	[85]	[92,131,65,177]	8	hSpCas9	negative selection	9274293
32087551	32087574	2	+	27260156	TOV112D	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.1836510504312629	[578]	[567,991,314,827]	3	hSpCas9	negative selection	9274294
32087509	32087532	2	+	27260156	TOV112D	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.05902469406528876	[68]	[77,70,74,43]	-1	hSpCas9	negative selection	9274295
32115741	32115764	2	-	27260156	TC71	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.0318219640696385	[341]	[349,343,367,441]	0	hSpCas9	negative selection	9331292
32114744	32114767	2	-	27260156	TC71	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.023034015818848008	[561]	[627,563,603,654]	0	hSpCas9	negative selection	9331293
32114811	32114834	2	-	27260156	TC71	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	-0.047185379454340204	[186]	[261,112,235,159]	-1	hSpCas9	negative selection	9331294
32115700	32115723	2	+	27260156	TC71	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.5304801293086143	[85]	[137,123,120,150]	9	hSpCas9	negative selection	9395544
32087551	32087574	2	+	27260156	TC71	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.07619155248308218	[578]	[696,601,542,785]	2	hSpCas9	negative selection	9395545
32087509	32087532	2	+	27260156	TC71	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.25702187442913527	[68]	[111,93,88,55]	6	hSpCas9	negative selection	9395546
32115741	32115764	2	-	27260156	TC32	viability	TTCTTACGAGTAGCAGTTGTAGG	SPAST	ENSG00000021574	0.06858781645149314	[341]	[356,181,555,327]	1	hSpCas9	negative selection	9452543
32114744	32114767	2	-	27260156	TC32	viability	CACTGTAACTAGGTGCTCTATGG	SPAST	ENSG00000021574	0.2611476591601565	[561]	[625,481,874,608]	4	hSpCas9	negative selection	9452544
32114811	32114834	2	-	27260156	TC32	viability	CAGAATACCTTATGAGTGGTAGG	SPAST	ENSG00000021574	0.12277220167545799	[186]	[207,128,265,184]	2	hSpCas9	negative selection	9452545
32115700	32115723	2	+	27260156	TC32	viability	AGGGTACTCCGAAAACAAATAGG	SPAST	ENSG00000021574	0.34779912610839947	[85]	[122,56,132,112]	6	hSpCas9	negative selection	9516795
32087551	32087574	2	+	27260156	TC32	viability	GGAATAGCTGTTATAGTTACAGG	SPAST	ENSG00000021574	0.3396292675041295	[578]	[706,455,946,736]	6	hSpCas9	negative selection	9516796
32087509	32087532	2	+	27260156	TC32	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.8476108918085643	[68]	[131,149,92,60]	9	hSpCas9	negative selection	9516797
32063997	32064020	2	-	27869803	HPAFII	viability after 27 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.26232399214536795	[863]	[468,607,NaN]	2	hSpCas9	negative selection	9574798
32064220	32064243	2	-	27869803	HPAFII	viability after 27 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.25987255717675106	[334]	[295,22,NaN]	-2	hSpCas9	negative selection	9574799
32098855	32098878	2	+	27869803	HPAFII	viability after 27 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	1.0170675870608639	[178]	[250,139,NaN]	8	hSpCas9	negative selection	9574800
32114745	32114768	2	+	27869803	HPAFII	viability after 27 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-1.3775644216798246	[504]	[105,98,NaN]	-7	hSpCas9	negative selection	9574801
32114773	32114796	2	-	27869803	HPAFII	viability after 27 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.1738819747042043	[505]	[237,233,NaN]	-1	hSpCas9	negative selection	9574802
32154451	32154474	2	+	27869803	HPAFII	viability after 27 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.7946080866402694	[603]	[342,723,NaN]	6	hSpCas9	negative selection	9574803
32063997	32064020	2	-	27869803	HPAFII	viability after 15 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.02959911702831186	[863]	[1273,744,NaN]	0	hSpCas9	negative selection	9657247
32064220	32064243	2	-	27869803	HPAFII	viability after 15 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.3588357382527329	[334]	[482,154,NaN]	-4	hSpCas9	negative selection	9657248
32098855	32098878	2	+	27869803	HPAFII	viability after 15 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.11047898255833588	[178]	[248,181,NaN]	1	hSpCas9	negative selection	9657249
32114745	32114768	2	+	27869803	HPAFII	viability after 15 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.40209328573160796	[504]	[472,371,NaN]	-4	hSpCas9	negative selection	9657250
32114773	32114796	2	-	27869803	HPAFII	viability after 15 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.2913411839487374	[505]	[682,295,NaN]	-3	hSpCas9	negative selection	9657251
32154451	32154474	2	+	27869803	HPAFII	viability after 15 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.31079521525448484	[603]	[983,693,NaN]	3	hSpCas9	negative selection	9657252
32063997	32064020	2	-	27869803	ASPC1	viability after 36 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.50917529976921	[1815]	[521,1383,776]	5	hSpCas9	negative selection	9739696
32064220	32064243	2	-	27869803	ASPC1	viability after 36 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.7764543945272668	[583]	[197,422,392]	7	hSpCas9	negative selection	9739697
32098855	32098878	2	+	27869803	ASPC1	viability after 36 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.7571154965961452	[560]	[277,82,26]	-6	hSpCas9	negative selection	9739698
32114745	32114768	2	+	27869803	ASPC1	viability after 36 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	0.5641640815169298	[1328]	[827,821,470]	5	hSpCas9	negative selection	9739699
32114773	32114796	2	-	27869803	ASPC1	viability after 36 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.20517762280120844	[967]	[322,589,272]	2	hSpCas9	negative selection	9739700
32154451	32154474	2	+	27869803	ASPC1	viability after 36 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	-0.4944038510635087	[1421]	[220,433,375]	-5	hSpCas9	negative selection	9739701
32063997	32064020	2	-	27869803	PATU8988S	viability after 35 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.0795017951286503	[1556]	[490,834,NaN]	0	hSpCas9	negative selection	9822145
32064220	32064243	2	-	27869803	PATU8988S	viability after 35 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.6414168068133282	[608]	[423,340,NaN]	7	hSpCas9	negative selection	9822146
32098855	32098878	2	+	27869803	PATU8988S	viability after 35 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	-0.6534194477089039	[329]	[83,84,NaN]	-6	hSpCas9	negative selection	9822147
32114745	32114768	2	+	27869803	PATU8988S	viability after 35 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.08157665223298483	[939]	[257,457,NaN]	-1	hSpCas9	negative selection	9822148
32114773	32114796	2	-	27869803	PATU8988S	viability after 35 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.304817693677201	[861]	[302,258,NaN]	-3	hSpCas9	negative selection	9822149
32154451	32154474	2	+	27869803	PATU8988S	viability after 35 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.6667724968487443	[1179]	[736,771,NaN]	7	hSpCas9	negative selection	9822150
32063997	32064020	2	-	27869803	PATU8988S	viability after 31 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	-0.0706566226900962	[1556]	[445,775,NaN]	-1	hSpCas9	negative selection	9904594
32064220	32064243	2	-	27869803	PATU8988S	viability after 31 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	0.8330692156905298	[608]	[447,412,NaN]	8	hSpCas9	negative selection	9904595
32098855	32098878	2	+	27869803	PATU8988S	viability after 31 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.03169246706816187	[329]	[75,208,NaN]	0	hSpCas9	negative selection	9904596
32114745	32114768	2	+	27869803	PATU8988S	viability after 31 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.14092825497413441	[939]	[225,484,NaN]	-1	hSpCas9	negative selection	9904597
32114773	32114796	2	-	27869803	PATU8988S	viability after 31 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.41561319748567904	[861]	[280,227,NaN]	-5	hSpCas9	negative selection	9904598
32154451	32154474	2	+	27869803	PATU8988S	viability after 31 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.6050674489579553	[1179]	[522,960,NaN]	7	hSpCas9	negative selection	9904599
32063997	32064020	2	-	27869803	HPAFII	viability after 35 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.3362316058693895	[863]	[467,646,NaN]	2	hSpCas9	negative selection	9987043
32064220	32064243	2	-	27869803	HPAFII	viability after 35 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.10543599412784194	[334]	[262,38,NaN]	0	hSpCas9	negative selection	9987044
32098855	32098878	2	+	27869803	HPAFII	viability after 35 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.8119086753236111	[178]	[207,103,NaN]	5	hSpCas9	negative selection	9987045
32114745	32114768	2	+	27869803	HPAFII	viability after 35 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-0.9570304180239055	[504]	[102,163,NaN]	-5	hSpCas9	negative selection	9987046
32114773	32114796	2	-	27869803	HPAFII	viability after 35 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	-0.18593316398770432	[505]	[243,203,NaN]	-1	hSpCas9	negative selection	9987047
32154451	32154474	2	+	27869803	HPAFII	viability after 35 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.47608923674461323	[603]	[239,633,NaN]	3	hSpCas9	negative selection	9987048
32063997	32064020	2	-	27869803	HPAFII	viability after 31 days	AGCGCGAAGCCTACAAACAGCGG	SPAST	ENSG00000021574	0.3135747614881592	[863]	[593,611,NaN]	2	hSpCas9	negative selection	10069492
32064220	32064243	2	-	27869803	HPAFII	viability after 31 days	CTCATCCTCATCGATGCGCAGGG	SPAST	ENSG00000021574	-0.08587660245324358	[334]	[291,51,NaN]	0	hSpCas9	negative selection	10069493
32098855	32098878	2	+	27869803	HPAFII	viability after 31 days	ACTAACTTGGCATGCCGCAATGG	SPAST	ENSG00000021574	0.7646935525998633	[178]	[196,141,NaN]	5	hSpCas9	negative selection	10069494
32114745	32114768	2	+	27869803	HPAFII	viability after 31 days	CATAGAGCACCTAGTTACAGTGG	SPAST	ENSG00000021574	-1.3668145716879585	[504]	[73,148,NaN]	-7	hSpCas9	negative selection	10069495
32114773	32114796	2	-	27869803	HPAFII	viability after 31 days	CTGTTTCACTCCAGAAACCATGG	SPAST	ENSG00000021574	0.244074517226615	[505]	[376,291,NaN]	1	hSpCas9	negative selection	10069496
32154451	32154474	2	+	27869803	HPAFII	viability after 31 days	AGCGTACATACGTTGGAACAAGG	SPAST	ENSG00000021574	0.7090248090739486	[603]	[366,757,NaN]	5	hSpCas9	negative selection	10069497
32063853	32063876	2	+	28162770	P31FUJ	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-1.511661577409855	[164]	[67]	-7	hSpCas9	negative selection	10241277
32087509	32087532	2	+	28162770	P31FUJ	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-8.215795779108689	[252]	[0]	-9	hSpCas9	negative selection	10241278
32063992	32064015	2	-	28162770	P31FUJ	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-1.0628772029720661	[31]	[17]	-6	hSpCas9	negative selection	10241279
32064068	32064091	2	-	28162770	P31FUJ	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.1453393631640392	[31]	[16]	-7	hSpCas9	negative selection	10241280
32136567	32136590	2	+	28162770	P31FUJ	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-4.474388190984137	[226]	[11]	-9	hSpCas9	negative selection	10241281
32064041	32064064	2	-	28162770	P31FUJ	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.966156545028206	[265]	[79]	-8	hSpCas9	negative selection	10241282
32141925	32141948	2	+	28162770	P31FUJ	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-3.602966484954589	[516]	[49]	-9	hSpCas9	negative selection	10241283
32144986	32145009	2	+	28162770	P31FUJ	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-4.148823811775025	[316]	[20]	-9	hSpCas9	negative selection	10241284
32064199	32064222	2	-	28162770	P31FUJ	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-5.929273020352525	[362]	[6]	-9	hSpCas9	negative selection	10241285
32087493	32087516	2	+	28162770	P31FUJ	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-2.782489230388004	[403]	[68]	-9	hSpCas9	negative selection	10241286
32063853	32063876	2	+	28162770	TF1	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	NaN	[NaN]	[70]	1	hSpCas9	negative selection	10410643
32087509	32087532	2	+	28162770	TF1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	NaN	[NaN]	[208]	1	hSpCas9	negative selection	10410644
32063992	32064015	2	-	28162770	TF1	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	NaN	[NaN]	[54]	1	hSpCas9	negative selection	10410645
32064068	32064091	2	-	28162770	TF1	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	NaN	[NaN]	[1]	1	hSpCas9	negative selection	10410646
32136567	32136590	2	+	28162770	TF1	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	NaN	[NaN]	[26]	1	hSpCas9	negative selection	10410647
32064041	32064064	2	-	28162770	TF1	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	NaN	[NaN]	[74]	1	hSpCas9	negative selection	10410648
32141925	32141948	2	+	28162770	TF1	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	NaN	[NaN]	[570]	1	hSpCas9	negative selection	10410649
32144986	32145009	2	+	28162770	TF1	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	NaN	[NaN]	[78]	1	hSpCas9	negative selection	10410650
32064199	32064222	2	-	28162770	TF1	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	NaN	[NaN]	[341]	1	hSpCas9	negative selection	10410651
32087493	32087516	2	+	28162770	TF1	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	NaN	[NaN]	[331]	1	hSpCas9	negative selection	10410652
32063853	32063876	2	+	28162770	SKM1	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.5171471164935846	[50]	[62]	-4	hSpCas9	negative selection	10580009
32087509	32087532	2	+	28162770	SKM1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.4948554073179348	[56]	[141]	5	hSpCas9	negative selection	10580010
32063992	32064015	2	-	28162770	SKM1	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-1.1699250014423126	[13]	[10]	-7	hSpCas9	negative selection	10580011
32064068	32064091	2	-	28162770	SKM1	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.4740783946016989	[10]	[6]	-7	hSpCas9	negative selection	10580012
32136567	32136590	2	+	28162770	SKM1	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-0.7118187802715826	[62]	[67]	-5	hSpCas9	negative selection	10580013
32064041	32064064	2	-	28162770	SKM1	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.2594070103293039	[64]	[95]	-2	hSpCas9	negative selection	10580014
32141925	32141948	2	+	28162770	SKM1	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	0.3634438887566849	[112]	[256]	3	hSpCas9	negative selection	10580015
32144986	32145009	2	+	28162770	SKM1	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	0.6768041589494362	[45]	[129]	6	hSpCas9	negative selection	10580016
32064199	32064222	2	-	28162770	SKM1	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	0.2051344319155085	[78]	[160]	2	hSpCas9	negative selection	10580017
32087493	32087516	2	+	28162770	SKM1	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.26560834949762013	[118]	[174]	-2	hSpCas9	negative selection	10580018
32063853	32063876	2	+	28162770	SKM1	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.5171471164935846	[50]	[62]	-4	hSpCas9	negative selection	10749375
32087509	32087532	2	+	28162770	SKM1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.4948554073179348	[56]	[141]	5	hSpCas9	negative selection	10749376
32063992	32064015	2	-	28162770	SKM1	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-1.1699250014423126	[13]	[10]	-7	hSpCas9	negative selection	10749377
32064068	32064091	2	-	28162770	SKM1	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.4740783946016989	[10]	[6]	-7	hSpCas9	negative selection	10749378
32136567	32136590	2	+	28162770	SKM1	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-0.7118187802715826	[62]	[67]	-5	hSpCas9	negative selection	10749379
32064041	32064064	2	-	28162770	SKM1	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.2594070103293039	[64]	[95]	-2	hSpCas9	negative selection	10749380
32141925	32141948	2	+	28162770	SKM1	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	0.3634438887566849	[112]	[256]	3	hSpCas9	negative selection	10749381
32144986	32145009	2	+	28162770	SKM1	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	0.6768041589494362	[45]	[129]	6	hSpCas9	negative selection	10749382
32064199	32064222	2	-	28162770	SKM1	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	0.2051344319155085	[78]	[160]	2	hSpCas9	negative selection	10749383
32087493	32087516	2	+	28162770	SKM1	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.26560834949762013	[118]	[174]	-2	hSpCas9	negative selection	10749384
32063853	32063876	2	+	28162770	PL21	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.7567916636187941	[197]	[50]	-5	hSpCas9	negative selection	10918741
32087509	32087532	2	+	28162770	PL21	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.6855664416595619	[159]	[111]	6	hSpCas9	negative selection	10918742
32063992	32064015	2	-	28162770	PL21	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-3.8222281985391344	[64]	[1]	-9	hSpCas9	negative selection	10918743
32064068	32064091	2	-	28162770	PL21	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	0.41773104956194707	[42]	[24]	3	hSpCas9	negative selection	10918744
32136567	32136590	2	+	28162770	PL21	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-2.505838287193202	[260]	[19]	-9	hSpCas9	negative selection	10918745
32064041	32064064	2	-	28162770	PL21	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.9697853869529922	[314]	[34]	-8	hSpCas9	negative selection	10918746
32141925	32141948	2	+	28162770	PL21	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-1.1217884803980422	[409]	[81]	-6	hSpCas9	negative selection	10918747
32144986	32145009	2	+	28162770	PL21	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-2.790815245907673	[158]	[9]	-9	hSpCas9	negative selection	10918748
32064199	32064222	2	-	28162770	PL21	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-3.7869213298031816	[221]	[6]	-9	hSpCas9	negative selection	10918749
32087493	32087516	2	+	28162770	PL21	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-1.7432768571443125	[299]	[38]	-8	hSpCas9	negative selection	10918750
32063853	32063876	2	+	28162770	P31FUJ	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-1.511661577409855	[164]	[67]	-7	hSpCas9	negative selection	11088107
32087509	32087532	2	+	28162770	P31FUJ	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-8.215795779108689	[252]	[0]	-9	hSpCas9	negative selection	11088108
32063992	32064015	2	-	28162770	P31FUJ	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-1.0628772029720661	[31]	[17]	-6	hSpCas9	negative selection	11088109
32064068	32064091	2	-	28162770	P31FUJ	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.1453393631640392	[31]	[16]	-7	hSpCas9	negative selection	11088110
32136567	32136590	2	+	28162770	P31FUJ	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-4.474388190984137	[226]	[11]	-9	hSpCas9	negative selection	11088111
32064041	32064064	2	-	28162770	P31FUJ	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.966156545028206	[265]	[79]	-8	hSpCas9	negative selection	11088112
32141925	32141948	2	+	28162770	P31FUJ	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-3.602966484954589	[516]	[49]	-9	hSpCas9	negative selection	11088113
32144986	32145009	2	+	28162770	P31FUJ	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-4.148823811775025	[316]	[20]	-9	hSpCas9	negative selection	11088114
32064199	32064222	2	-	28162770	P31FUJ	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-5.929273020352525	[362]	[6]	-9	hSpCas9	negative selection	11088115
32087493	32087516	2	+	28162770	P31FUJ	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-2.782489230388004	[403]	[68]	-9	hSpCas9	negative selection	11088116
32063853	32063876	2	+	28162770	OCIAML5	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.4476763157846616	[101]	[134]	-3	hSpCas9	negative selection	11257473
32087509	32087532	2	+	28162770	OCIAML5	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-1.2181944696618945	[115]	[89]	-6	hSpCas9	negative selection	11257474
32063992	32064015	2	-	28162770	OCIAML5	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-0.4370290715851535	[35]	[47]	-3	hSpCas9	negative selection	11257475
32064068	32064091	2	-	28162770	OCIAML5	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.790666026199854	[45]	[23]	-7	hSpCas9	negative selection	11257476
32136567	32136590	2	+	28162770	OCIAML5	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-5.71831518197517	[174]	[5]	-9	hSpCas9	negative selection	11257477
32064041	32064064	2	-	28162770	OCIAML5	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.0650602941981955	[101]	[87]	-6	hSpCas9	negative selection	11257478
32141925	32141948	2	+	28162770	OCIAML5	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-0.47754764861132437	[333]	[432]	-3	hSpCas9	negative selection	11257479
32144986	32145009	2	+	28162770	OCIAML5	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-1.7479581675232583	[146]	[78]	-7	hSpCas9	negative selection	11257480
32064199	32064222	2	-	28162770	OCIAML5	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-0.5771253983460288	[180]	[218]	-4	hSpCas9	negative selection	11257481
32087493	32087516	2	+	28162770	OCIAML5	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.5968095156219223	[278]	[332]	-4	hSpCas9	negative selection	11257482
32063853	32063876	2	+	28162770	EOL1	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.7758919465705577	[324]	[99]	-5	hSpCas9	negative selection	11426839
32087509	32087532	2	+	28162770	EOL1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.36189123859820305	[260]	[106]	-3	hSpCas9	negative selection	11426840
32063992	32064015	2	-	28162770	EOL1	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.9617007699880007	[114]	[117]	8	hSpCas9	negative selection	11426841
32064068	32064091	2	-	28162770	EOL1	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.6318455769538507	[99]	[16]	-7	hSpCas9	negative selection	11426842
32136567	32136590	2	+	28162770	EOL1	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-1.4504916597763902	[414]	[79]	-7	hSpCas9	negative selection	11426843
32064041	32064064	2	-	28162770	EOL1	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.8022579563754499	[565]	[170]	-5	hSpCas9	negative selection	11426844
32141925	32141948	2	+	28162770	EOL1	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-0.9552865256077261	[448]	[121]	-6	hSpCas9	negative selection	11426845
32144986	32145009	2	+	28162770	EOL1	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-0.13864605491665938	[279]	[133]	-1	hSpCas9	negative selection	11426846
32064199	32064222	2	-	28162770	EOL1	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-0.48282802600024255	[312]	[117]	-4	hSpCas9	negative selection	11426847
32087493	32087516	2	+	28162770	EOL1	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.5365861422977632	[423]	[153]	-4	hSpCas9	negative selection	11426848
32063853	32063876	2	+	28162770	MOLM13	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.004415318407115509	[196]	[141]	0	hSpCas9	negative selection	11596205
32087509	32087532	2	+	28162770	MOLM13	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.16802430662333281	[316]	[203]	-1	hSpCas9	negative selection	11596206
32063992	32064015	2	-	28162770	MOLM13	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-0.10365260341425597	[106]	[71]	0	hSpCas9	negative selection	11596207
32064068	32064091	2	-	28162770	MOLM13	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	0.10531930215987062	[53]	[41]	0	hSpCas9	negative selection	11596208
32136567	32136590	2	+	28162770	MOLM13	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-2.9013444281211402	[340]	[32]	-8	hSpCas9	negative selection	11596209
32064041	32064064	2	-	28162770	MOLM13	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.5014138212325046	[371]	[94]	-6	hSpCas9	negative selection	11596210
32141925	32141948	2	+	28162770	MOLM13	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-1.2377701434436743	[622]	[190]	-6	hSpCas9	negative selection	11596211
32144986	32145009	2	+	28162770	MOLM13	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-1.1857858692605916	[257]	[81]	-6	hSpCas9	negative selection	11596212
32064199	32064222	2	-	28162770	MOLM13	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-1.6938616884520332	[527]	[117]	-7	hSpCas9	negative selection	11596213
32087493	32087516	2	+	28162770	MOLM13	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.6191823264402796	[460]	[216]	-4	hSpCas9	negative selection	11596214
32063853	32063876	2	+	28162770	HEL	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	0.1692228674557839	[192]	[138]	1	hSpCas9	negative selection	11765571
32087509	32087532	2	+	28162770	HEL	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.04105893633426708	[216]	[142]	0	hSpCas9	negative selection	11765572
32063992	32064015	2	-	28162770	HEL	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	1.0268823233334172	[58]	[76]	8	hSpCas9	negative selection	11765573
32064068	32064091	2	-	28162770	HEL	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-0.7134049782249183	[63]	[24]	-5	hSpCas9	negative selection	11765574
32136567	32136590	2	+	28162770	HEL	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-2.8210579791983426	[330]	[29]	-8	hSpCas9	negative selection	11765575
32064041	32064064	2	-	28162770	HEL	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.164616090057247	[384]	[109]	-6	hSpCas9	negative selection	11765576
32141925	32141948	2	+	28162770	HEL	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-1.3934347805531282	[524]	[127]	-7	hSpCas9	negative selection	11765577
32144986	32145009	2	+	28162770	HEL	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-1.8865142361345117	[253]	[43]	-7	hSpCas9	negative selection	11765578
32064199	32064222	2	-	28162770	HEL	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-0.5940446816417124	[402]	[170]	-4	hSpCas9	negative selection	11765579
32087493	32087516	2	+	28162770	HEL	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-1.1922019217291466	[395]	[110]	-6	hSpCas9	negative selection	11765580
32063853	32063876	2	+	28162770	MV411	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.8374513468037379	[139]	[65]	-5	hSpCas9	negative selection	11934937
32087509	32087532	2	+	28162770	MV411	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.24374280361776393	[200]	[142]	-2	hSpCas9	negative selection	11934938
32063992	32064015	2	-	28162770	MV411	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	0.04390415669764325	[37]	[32]	0	hSpCas9	negative selection	11934939
32064068	32064091	2	-	28162770	MV411	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.2809414215720132	[74]	[25]	-7	hSpCas9	negative selection	11934940
32136567	32136590	2	+	28162770	MV411	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-1.8357036845174706	[249]	[58]	-8	hSpCas9	negative selection	11934941
32064041	32064064	2	-	28162770	MV411	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.8487777644765278	[247]	[115]	-5	hSpCas9	negative selection	11934942
32141925	32141948	2	+	28162770	MV411	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	0.11093555087791329	[287]	[261]	0	hSpCas9	negative selection	11934943
32144986	32145009	2	+	28162770	MV411	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-0.5384376438643772	[149]	[86]	-4	hSpCas9	negative selection	11934944
32064199	32064222	2	-	28162770	MV411	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-1.2092008529628413	[257]	[93]	-7	hSpCas9	negative selection	11934945
32087493	32087516	2	+	28162770	MV411	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-1.2067383424030267	[410]	[149]	-7	hSpCas9	negative selection	11934946
32063853	32063876	2	+	28162770	MONOMAC1	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	0.7592417755111285	[133]	[259]	7	hSpCas9	negative selection	12104303
32087509	32087532	2	+	28162770	MONOMAC1	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	0.5140501152810468	[134]	[220]	5	hSpCas9	negative selection	12104304
32063992	32064015	2	-	28162770	MONOMAC1	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-0.2662995094966675	[63]	[60]	-2	hSpCas9	negative selection	12104305
32064068	32064091	2	-	28162770	MONOMAC1	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.4449643605031395	[37]	[15]	-8	hSpCas9	negative selection	12104306
32136567	32136590	2	+	28162770	MONOMAC1	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-1.6215346755874638	[152]	[56]	-8	hSpCas9	negative selection	12104307
32064041	32064064	2	-	28162770	MONOMAC1	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.5290186066108389	[179]	[142]	-4	hSpCas9	negative selection	12104308
32141925	32141948	2	+	28162770	MONOMAC1	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	0.28283402315074424	[222]	[310]	3	hSpCas9	negative selection	12104309
32144986	32145009	2	+	28162770	MONOMAC1	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-0.1823900710951522	[97]	[98]	-1	hSpCas9	negative selection	12104310
32064199	32064222	2	-	28162770	MONOMAC1	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-0.5702541706437705	[135]	[104]	-5	hSpCas9	negative selection	12104311
32087493	32087516	2	+	28162770	MONOMAC1	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	0.10447170658887572	[184]	[227]	1	hSpCas9	negative selection	12104312
32063853	32063876	2	+	28162770	OCIAML2	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-1.089200718606137	[37]	[98]	-6	hSpCas9	negative selection	12273669
32087509	32087532	2	+	28162770	OCIAML2	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-4.903589232518267	[26]	[4]	-9	hSpCas9	negative selection	12273670
32063992	32064015	2	-	28162770	OCIAML2	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-0.8461389603343679	[11]	[36]	-5	hSpCas9	negative selection	12273671
32064068	32064091	2	-	28162770	OCIAML2	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-1.4706298252421615	[5]	[11]	-7	hSpCas9	negative selection	12273672
32136567	32136590	2	+	28162770	OCIAML2	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-6.025218676919799	[46]	[3]	-9	hSpCas9	negative selection	12273673
32064041	32064064	2	-	28162770	OCIAML2	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-1.4288096495475342	[50]	[104]	-7	hSpCas9	negative selection	12273674
32141925	32141948	2	+	28162770	OCIAML2	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-3.314510623324879	[69]	[38]	-9	hSpCas9	negative selection	12273675
32144986	32145009	2	+	28162770	OCIAML2	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-5.930061443879459	[43]	[3]	-9	hSpCas9	negative selection	12273676
32064199	32064222	2	-	28162770	OCIAML2	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	-7.9941917812991745	[45]	[0]	-9	hSpCas9	negative selection	12273677
32087493	32087516	2	+	28162770	OCIAML2	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-3.18683685924157	[68]	[41]	-9	hSpCas9	negative selection	12273678
32063853	32063876	2	+	28162770	OCIAML3	viability	GGGAAGAAGAAAGGCTCCGGCGG	SPAST	ENSG00000021574	-0.06057544024308903	[37]	[109]	0	hSpCas9	negative selection	12443035
32087509	32087532	2	+	28162770	OCIAML3	viability	GCTGTGGAATGGTATAAGAAAGG	SPAST	ENSG00000021574	-0.51600512832289	[71]	[151]	-4	hSpCas9	negative selection	12443036
32063992	32064015	2	-	28162770	OCIAML3	viability	GAAGCCTACAAACAGCGGGTAGG	SPAST	ENSG00000021574	-1.3460801268805778	[15]	[18]	-7	hSpCas9	negative selection	12443037
32064068	32064091	2	-	28162770	OCIAML3	viability	TGAGGGCGCGGGAGAAGCGCTGG	SPAST	ENSG00000021574	-0.7575063726070428	[13]	[24]	-5	hSpCas9	negative selection	12443038
32136567	32136590	2	+	28162770	OCIAML3	viability	GAGAAGGAGAGAAATTGGTGAGG	SPAST	ENSG00000021574	-1.906597870632189	[76]	[61]	-8	hSpCas9	negative selection	12443039
32064041	32064064	2	-	28162770	OCIAML3	viability	GCCACACGAAGAGGAGCCCCAGG	SPAST	ENSG00000021574	-0.09831247770009437	[49]	[140]	-1	hSpCas9	negative selection	12443040
32141925	32141948	2	+	28162770	OCIAML3	viability	TGTGTCTTTACCAAATGAGGAGG	SPAST	ENSG00000021574	-0.06302276066183188	[117]	[340]	0	hSpCas9	negative selection	12443041
32144986	32145009	2	+	28162770	OCIAML3	viability	GCAGCACTGGGTCCTATCCGAGG	SPAST	ENSG00000021574	-1.5642602969301111	[47]	[48]	-8	hSpCas9	negative selection	12443042
32064199	32064222	2	-	28162770	OCIAML3	viability	GGCAATGGAGATGTACTCGAAGG	SPAST	ENSG00000021574	0.11433727590227524	[55]	[182]	1	hSpCas9	negative selection	12443043
32087493	32087516	2	+	28162770	OCIAML3	viability	AGGACAGAAGGAGCAAGCTGTGG	SPAST	ENSG00000021574	-0.07317547702272276	[91]	[263]	0	hSpCas9	negative selection	12443044