RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 117332
  • Symbol: Rheb
  • Description: Ras homolog enriched in brain
DataSource: http://genomernai.dkfz.de/v16/genedetails/117332

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00001-A 117332 FBgn0041191 Rheb DRSC12148 -2.52 Decreased cell size yes Cell size regulation PDGF/VEGF signaling controls cell size in Drosophila. Sims et al. 2009 19216764 Cell line S2R+ F-actin and microtubule protein expression and DNA content Fluorescence rp Genome-wide dsRNA Cell area Z-score < -2
GR00002-A 117332 FBgn0041191 Rheb DRSC12148 1.777877965 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 117332 FBgn0041191 Rheb DRSC12148 3.660486112 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00007-A-0 117332 FBgn0041191 DRSC12148 np Defasciculation no Neural outgrowth Identification of neural outgrowth genes using genome-wide RNAi. Sepp et al. 2008 18604272 Cell line Primary neural cells Morphology High content (microscopy) DRSC dsRNA Visual inspection np
GR00021-A-0 117332 FBgn0041191 DRSC12148 strong ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 117332 HFA12148 0.6 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 117332 HFA12148 1.9 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00047-A-1 117332 FBgn0041191 Rheb DRSC12148 sp Decreased horseradish peroxidase protein expression no Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
117332 FBgn0041191 Rheb GH10361 sp none no library: DGC1
GR00073-A-0 117332 FBgn0041191 CG1081 np np Chromosome misalignment no Mitosis Genes required for mitotic spindle assembly in Drosophila S2 cells. Goshima et al. 2007 17412918 Cell line S2 Mitotic Spindle Assembly High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00128-A-2 117332 FBgn0041191 CG1081 DRSC30919 sp none no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00130-A 117332 FBgn0041191 Rheb np <= -2 Decreased mutant human huntingtin aggregation yes Mutant human huntingtin aggregation RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. Doumanis et al. 2009 19789644 Cell line BG2-c2 Nhtt(62Q)EGFP aggregate number and size Fluorescence OpenBiosystems RNAi library Selected genes dsRNA Z-score Suppressor: <
GR00169-A 117332 FBgn0041191 Rheb DRSC12148 2.1 none 10 min insulin stimulation yes Akt-TOR pathway negative feedback regulation Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. Kockel et al. 2010 20585550 Cell line Kc167 dAkt phosphorylation Fluorescence Custom-made Genome-wide dsRNA Z-score < -2.5 OR > 2.5
GR00169-A 117332 FBgn0041191 Rheb DRSC12148 3.2 Increased dAkt phosphorylation without insulin stimulation yes Akt-TOR pathway negative feedback regulation Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. Kockel et al. 2010 20585550 Cell line Kc167 dAkt phosphorylation Fluorescence Custom-made Genome-wide dsRNA Z-score < -2.5 OR > 2.5
GR00170-A-1 117332 Rheb DRSC12148 -3.8 Decreased HIF dependent transcription with desferrioxamine yes HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00170-A-2 117332 Rheb DRSC30919 45.4 none no desferrioxamine inhibition < 50 % HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-2 117332 Rheb DRSC30920 37.8 none no desferrioxamine inhibition < 50 % HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-3 117332 dRheb DRSC30920 82.1 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00170-A-3 117332 dRheb DRSC30919 54.6 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00194-A 117332 CG1081 Rheb np np none no no. of corners: bottom 40 S2 cell spreading A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. D'Ambrosio and Vale 2010 21041442 Cell line S2U alpha-tubulin and actin protein expression Fluorescence V2 RNAi library Genome-wide dsRNA Complex, sp Complex criteria
GR00214-A-1 117332 FBgn0041191 Rheb DRSC12148 0.37 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 117332 FBgn0041191 Rheb DRSC12148 -1.34 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 117332 FBgn0041191 Rheb DRSC12148 0.55 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 117332 FBgn0041191 Rheb DRSC12148 0.98 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00217-A-1 117332 CG1081 Rheb np medium Decreased WgRluc and not changed sFluc protein expression no Wg pathway regulation (1) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Cell line S2R+ WgRluc and sFluc protein expression Luminescence np Genome-wide dsRNA Phenotype strength Complex criteria Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00218-S 117332 CG1081 AMB32895 0.62 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 117332 CG1081 1081R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00308-A 117332 CG1081 Rheb np 2.67 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 117332 FBgn0041191 Rheb DRSC12148 np Other cellular phenotype decreased cell size Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A 117332 FBgn0041191 Rheb HMS00923 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
117332 FBgn0041191 np np sp none
GR00388-A 117332 CG1081 BL33966 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.