RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 31118
• Symbol: dor
• Description: deep orange

DataSource: http://genomernai.dkfz.de/v16/genedetails/31118

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	31118	FBgn0000482	dor	DRSC18765	1.317399219	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31118	FBgn0000482	dor	DRSC18765	0	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00007-A-0	31118	FBgn0000482		DRSC18765	np	Defasciculation		no		Neural outgrowth	Identification of neural outgrowth genes using genome-wide RNAi.	Sepp et al.	2008	18604272	Cell line	Primary neural cells	Morphology	High content (microscopy)	DRSC		dsRNA	Visual inspection	np
GR00031-A-1	31118			HFA18765	-0.6	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	31118			HFA18765	-0.3	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00038-A-0	31118	FBgn0000482	dor	np	****	Escape pff LLO- strain		no		L. monocytogenes infection	Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen.	Cheng et al.	2005	16157870	Cell line	S2	L. monocytogenes infection	High content (microscopy)	Custom-made		dsRNA	Visual inspection	np
	31118	FBgn0000482, FBgn0023530	dor, CG3740	SD04291	sp	none		no	library: DGC1
GR00135-A-1	31118	CG3093	dor	33733	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1	31118	CG3093	dor	33734	1.31	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	31118	CG3093		33733	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00138-A-1	31118	CG3093		33734	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00142-A-1	31118	CG3093	dor	33733	0.202380952380952	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	31118	CG3093		33733	sp	Completely lethal (pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	31118	CG3093		33734	sp	Completely lethal (pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00159-A	31118		dor	DRSC18765	np	none		no		Muscle assembly and maintenance	RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance.	Bai et al.	2008	18359903	Primary cells	primary embryonic cells	Muscle cell morphology	Fluorescence	np	Selected and random genes	dsRNA	Percentage of muscles with a given phenotype	Severe: > 80 %; medium: ~ 50 %
GR00189-A-3	31118	FBgn0000482	dor	np	np	Upregulation of Notch pathway		yes		Notch pathway regulation (3)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	GMR-GAL4	Notch pathway reporter	Fluorescence	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Average fluorescent intensity	Complex criteria
GR00189-A-4	31118	FBgn0000482	dor	33733	np	none		no	en-GAL4	Notch pathway regulation (4)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4 and C96-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	np	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-4	31118	FBgn0000482	dor	33734	np	none		no	en-GAL4	Notch pathway regulation (4)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4 and C96-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	np	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-4	31118	FBgn0000482	dor	33733	sp	Adult wing posterior compartment: smaller, crumbled, opaque, blisters		no	en-GAL4	Notch pathway regulation (4)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4 and C96-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	np	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-4	31118	FBgn0000482	dor	33734	sp	Upregulation of Notch pathway; adult wing posterior compartment: smaller, crumbled; adult wing posterior compartment: opaque; wing disc NRE:EGFP: up, ectopic; wing disc NRE:EGFP: gap		no	en-GAL4	Notch pathway regulation (4)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4 and C96-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	np	Selected genes	UAS-IR construct	Phenotype strength	np
GR00190-A-1	31118	FBgn0000482	dor	33733	0.225	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00216-A	31118	FBgn0000482	dor	BKN22242	0.9	none		no		Wnt proteins secretion	p24 proteins are required for secretion of Wnt ligands.	Buechling et al.	2011	22094269	Cell line	S2R+	Wg pathway reporter	Luminescence	BKN	Cellular transporting machinery genes	dsRNA	Z-score	> 2.5 OR < -2.5	Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S	31118	CG3093	dor	AMB27154	0.81	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	31118	CG3093		3093R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	31118	CG3093	dor	33734	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	31118	CG3093	dor	33733	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	31118	CG3093	dor	107053	0.033668303676923805	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	31118	FBgn0000482	dor	DRSC18765	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	31118	CG3093	dor	107053	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00326-A	31118	FBgn0000482	dor	3093	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00330-A	31118		dor	DRSC27554	np	Decreased androgen receptor (AR) reporter expression				Androgen receptor (AR) regulation	A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells	Imberg-Kazdan et al.	2013	23403032	Cell line	S2R+	Androgen receptor (AR) reporter	Luminescence	Whole Genome Drosophila Library (DRSC 2.0)	Genome-wide	dsRNA	Complex, sp	Complex criteria	Reporter expression was measured after stimulation with 10 nM of synthetic androgen. Results from the primary screen have been deposited with the Drosophila RNAi Screening Center (DRSC) (http://www.flyrnai. org) under the title ‘‘Factors affecting human androgen receptor transcriptional activation’’.
	31118	FBgn0000482	np	np	sp	none
GR00367-S	31118	CG3093		107053	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	31118	CG3093		107053	-0.27437500939877024	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A	31118			107053	0.4	none				Regulation of olfactory memory formation	Identification of genes that promote or inhibit olfactory memory formation in Drosophila.	Walkinshaw et al.	2015	25644700	Organism	Nsyb-GAL4	T-maze performance	Visual inspection	VDRC	Selected genes	UAS-IR construct	Performance index, sp	High performer in olfactory memory formation: >	Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A	31118	CG3093		GD33734		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.