Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 31381
Symbol: rb
Description: ruby

DataSource: http://genomernai.dkfz.de/v16/genedetails/31381

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	31381	FBgn0003210	rb	DRSC18826	-0.009748258	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31381	FBgn0003210	rb	DRSC18826	0.294095198	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	31381			HFA18826	-0.9	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	31381			HFA18826	-0.7	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	31381	FBgn0003210	rb	RE01344	sp	none		no	library: DGC2
GR00134-A-1	31381	CG11427	rb	38503	np	Lethal		no	early pupal	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	31381	CG11427	rb	38504	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	31381	CG11427	rb	38503	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies Î±2Î´3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1	31381	CG11427	rb	38504	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies Î±2Î´3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00137-A-1	31381	CG11427	Ruby	np	3.8	Increased dVMAT protein cell surface expression		yes		Regulated secretory pathway biogenesis (1)	RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway.	Asensio et al.	2010	21149569	Cell line	S2	dVMAT protein cell surface expression	Flow cytometry	DmRNAi library version 1	Selected genes	dsRNA	Z-score	>
GR00137-A-2	31381	CG11427	Ruby	np	np	Increased dVMAT protein cell surface expression		yes	no effect on DE/AA dVMAT protein cell surface expression	Regulated secretory pathway biogenesis (2)	RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway.	Asensio et al.	2010	21149569	Cell line	S2	dVMAT protein cell surface expression	Flow cytometry	Custom-made	Selected genes	dsRNA	Cumulative frequency distribution of fluorescence ratios	p-value < 0.0025 in at least 2 of 4 independent experiments
GR00138-A-1	31381	CG11427		38503	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCÎ”4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00139-A	31381		rb	38504	sp	Eye colour: orange; semi-lethal		no		Fly viability and adult morphology	A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.	Dietzl et al.	2007	17625558	Organism	Act5C-GAL4	Adult morphology and viability	Visual inspection	Custom-made	Selected and random genes	UAS-IR construct	Phenotype strength	np	Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00139-A	31381		rb	38503	sp	Eye colour: orange; semi-lethal		no		Fly viability and adult morphology	A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.	Dietzl et al.	2007	17625558	Organism	Act5C-GAL4	Adult morphology and viability	Visual inspection	Custom-made	Selected and random genes	UAS-IR construct	Phenotype strength	np	Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1	31381	CG11427	rb	38503	0.0303030303030303	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	31381	CG11427		38503	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	31381	CG11427		38504	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	31381	FBgn0003210	rb	38503	-0.372	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00214-A-1	31381	FBgn0003210	rb	DRSC27940	-0.02	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	31381	FBgn0003210	rb	DRSC27940	0.12	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	31381	FBgn0003210	rb	DRSC27940	0.33	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	31381	FBgn0003210	rb	DRSC27940	2.04	Increased ERK phosphorylation after sSpitz and EGF stimulation	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00216-A	31381	FBgn0003210	rb	BKN22917	1.3	none		no		Wnt proteins secretion	p24 proteins are required for secretion of Wnt ligands.	Buechling et al.	2011	22094269	Cell line	S2R+	Wg pathway reporter	Luminescence	BKN	Cellular transporting machinery genes	dsRNA	Z-score	> 2.5 OR < -2.5	Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S	31381	CG11427	rb	AMB17856	0.78	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	31381	CG11427		11427R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	31381	CG11427	rb	38503	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	31381	CG11427	rb	38504	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	31381	CG11427	rb	38504	0.32686745471371403	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	31381	FBgn0003210	rb	DRSC18826	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	31381	FBgn0003210	rb	HMS00479	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	31381	FBgn0003210	rb	JF03083	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	31381	FBgn0003210	np	np	sp	none
GR00367-S	31381	CG11427		38503	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	31381	CG11427		38504	-0.35350448158867476	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	31381	CG11427		GD38504		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	31381	CG11427		BL28668		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	31381	CG11427		BL32477		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.