Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 32078
Symbol: dsh
Description: dishevelled

DataSource: http://genomernai.dkfz.de/v16/genedetails/32078

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	32078	FBgn0000499	dsh	DRSC19803	-1.05768635	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32078	FBgn0000499	dsh	DRSC19803	1.236322546	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32078	FBgn0000499	dsh	DRSC19803	-0.60558881	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	32078			HFA19803	0.4	none	yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	32078			HFA19803	0.1	none	no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	32078	FBgn0000499	dsh	LD32934	sp	Decreased G1 DNA content, decreased viability, increased cytokinesis	yes	library: DGC2
GR00048-A-2	32078	FBgn0000499	dsh	np	1.019	none	yes		Cell size and cell-cycle regulation (2)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Proliferation and viability	Colorimetrics		Selected genes	dsRNA	MTS metabolic activity	> 2.5 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3	32078	FBgn0000499	dsh	np	422	none	yes		Cell size and cell-cycle regulation (3)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Cell number	Fluorescence		Selected genes	dsRNA	Average cell number per field	> 2 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-4	32078	FBgn0000499	dsh	np	below threshold	none	no		Cell size and cell-cycle regulation (4)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Number of mitotic cells	Fluorescence		Selected genes	dsRNA	Percentage	Visual inspection	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00128-A-2	32078	FBgn0000499	CG18361	DRSC33012	sp	Synthetic lethal with methyl methanesulphonate	no		Combinatorial effect with methyl methanesulphonate (2)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Selected genes	dsRNA	rp	rp
GR00216-A	32078	FBgn0000499	dsh	BKN28967	-3.3	Downregulation of Wg pathway	no	Wnt/Wg component	Wnt proteins secretion	p24 proteins are required for secretion of Wnt ligands.	Buechling et al.	2011	22094269	Cell line	S2R+	Wg pathway reporter	Luminescence	BKN	Cellular transporting machinery genes	dsRNA	Z-score	> 2.5 OR < -2.5	Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S	32078	CG18361	dsh	AMB27380	-1.35	none	no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	32078	CG18361		18361R	np	Lethal	no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	32078	CG18361	dsh	101525	np	none			piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00311-A	32078	FBgn0000499	dsh	DRSC19803	np	none			Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	32078	CG18361	dsh	101525	np	none			Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00326-A	32078	FBgn0000499	dsh	18361	np	none			Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00335-A	32078	FBgn0000499	dsh	JF01254	np	none			Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	32078	FBgn0000499	dsh	JF01253	np	none			Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	32078	FBgn0000499	np	np	sp	none
GR00367-S	32078	CG18361		101525	np	none		VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00385-A	32078			101525	0.23	Low performer in olfactory memory formation			Regulation of olfactory memory formation	Identification of genes that promote or inhibit olfactory memory formation in Drosophila.	Walkinshaw et al.	2015	25644700	Organism	Nsyb-GAL4	T-maze performance	Visual inspection	VDRC	Selected genes	UAS-IR construct	Performance index, sp	High performer in olfactory memory formation: >	Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A	32078	CG18361		KK101525		none			Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.