| GR00002-A |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
3.964404858 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
0.748099342 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
1.794506995 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
-1.306689615 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00004-A-0 |
32268 |
|
Bap60 |
DRSC19337 |
0.59 |
Endo-siRNA reporter downregulated |
|
no |
|
Drosophila small RNA pathways |
Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. |
Zhou et al. |
2008 |
19026789 |
Cell line |
S2 |
miRNA and siRNA pathway activity |
Dual luciferase |
DRSC |
|
dsRNA |
Fold change endo-siRNA reporter |
1.5/0.6 |
|
GR00004-A-0 |
32268 |
|
Bap60 |
DRSC19337 |
0.68 |
siRNA reporter downregulated |
|
no |
|
Drosophila small RNA pathways |
Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. |
Zhou et al. |
2008 |
19026789 |
Cell line |
S2 |
miRNA and siRNA pathway activity |
Dual luciferase |
DRSC |
|
dsRNA |
Fold change endo-siRNA reporter |
1.5/0.6 |
|
GR00031-A-1 |
32268 |
|
|
HFA19337 |
1.1 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
32268 |
|
|
HFA19337 |
0.3 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00037-A-1 |
32268 |
|
Bap60 |
np |
> 3 |
Upregulation of JAK/STAT pathway |
|
no |
|
JAK/STAT pathway regulation (1) |
Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. |
Baeg et al. |
2005 |
16055650 |
Cell line |
S2-NP |
JAK/STAT pathway reporter |
Luminescence |
rp |
Genome-wide |
dsRNA |
Fold change of relative luciferase units |
Complex criteria |
|
GR00038-A-0 |
32268 |
FBgn0025463 |
Bap60 |
np |
** |
Less vacuolar escape, fewer bacteria per cell |
|
no |
|
L. monocytogenes infection |
Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. |
Cheng et al. |
2005 |
16157870 |
Cell line |
S2 |
L. monocytogenes infection |
High content (microscopy) |
Custom-made |
|
dsRNA |
Visual inspection |
np |
|
GR00047-A-1 |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
sp |
Decreased horseradish peroxidase protein expression |
|
no |
|
Constitutive protein secretion and Golgi organization (1) |
Functional genomics reveals genes involved in protein secretion and Golgi organization. |
Bard et al. |
2006 |
16452979 |
Cell line |
S2 |
Horseradish peroxidase protein expression |
Luminescence |
|
Genome-wide |
dsRNA |
Z-score |
< -1.5 |
|
|
32268 |
FBgn0025463 |
Bap60 |
LD09078 |
sp |
none |
|
no |
library: DGC1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00065-A |
32268 |
CG4303 |
Bap60 |
Bap60 |
np |
Misrouting |
|
yes |
|
Dendrite pattern formation |
Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. |
Parrish et al. |
2006 |
16547170 |
Tissue |
GAL4221 |
mCD8 protein expression |
Fluorescence |
Custom-made |
Transcription factors |
UAS-IR construct |
np |
Phenotypes in multiple blind tests |
Additional information about secondary screens |
GR00092-A |
32268 |
FBgn0025463 |
CG4303 |
RDM1190-7299415 |
np |
none |
|
no |
|
Microtubule assembly and organization |
Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. |
Ducat et al. |
2008 |
18463163 |
Cell line |
S2 |
DNA content, alpha-tubulin and CP309 protein expression |
Fluorescence |
OpenBiosystems Drosophila RNAi Library 1.0 |
Mitotic regulator genes |
dsRNA |
Complex, sp |
Complex criteria |
|
GR00130-A |
32268 |
FBgn0025463 |
Bap60 |
np |
1.5 - 2 |
Increased mutant human huntingtin aggregation |
|
no |
|
Mutant human huntingtin aggregation |
RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. |
Doumanis et al. |
2009 |
19789644 |
Cell line |
BG2-c2 |
Nhtt(62Q)EGFP aggregate number and size |
Fluorescence |
OpenBiosystems RNAi library |
Selected genes |
dsRNA |
Z-score |
Suppressor: < |
|
GR00131-A-1 |
32268 |
|
Bap60 |
DRSC19337 |
> 75 |
Altered mitochondrial Ca2+ and/or H+ levels |
|
no |
|
Mitochondrial Ca2+/H+ antiporter regulation (1) |
Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. |
Jiang et al. |
2009 |
19797662 |
Cell line |
S2 |
mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) |
Fluorescence |
np |
Genome-wide |
dsRNA |
Percentage inhibition |
Class I: > 75; class II: 50-75; class III: 25–50 |
|
GR00134-A-1 |
32268 |
CG4303 |
Bap60 |
12673 |
np |
Lethal |
|
yes |
early pupal |
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00134-A-1 |
32268 |
CG4303 |
Bap60 |
12675 |
np |
Lethal |
|
yes |
early pupal |
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00134-A-2 |
32268 |
CG4303 |
Bap60 |
np |
np |
Wing posture / locomotion / ability to fly defect or lethal / semi-lethal |
|
no |
|
Muscle morphogenesis and function (2) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
Custom-made |
Selected genes |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00135-A-1 |
32268 |
CG4303 |
Bap60 |
12675 |
0 |
Developmentally lethal |
|
no |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-1 |
32268 |
CG4303 |
Bap60 |
12673 |
0 |
Developmentally lethal |
|
no |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00138-A-1 |
32268 |
CG4303 |
|
12673 |
0.5 |
Embryonic lethal or no offspring |
|
no |
|
Heart development and function (1) |
A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. |
Neely et al. |
2010 |
20371351 |
Tissue |
TinCΔ4 12a-Gal4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Developmental lethality |
< |
|
GR00142-A-1 |
32268 |
CG4303 |
Bap60 |
12673 |
0.580645161290322 |
none |
|
no |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00144-A-4 |
32268 |
CG4303 |
|
12673 |
sp |
Completely lethal (before pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-4 |
32268 |
CG4303 |
|
12675 |
sp |
Completely lethal (pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00170-A-1 |
32268 |
|
Bap60 |
DRSC19337 |
-4.1 |
Decreased HIF dependent transcription with desferrioxamine |
|
yes |
|
HIF-dependent transcription (1) |
Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. |
Dekanty et al. |
2010 |
20585616 |
Cell line |
S2 |
Hypoxia inducible HRE reporter |
Luminescence |
DRSC 1.0 library |
Genome-wide |
dsRNA |
Z-score |
< |
|
GR00170-A-2 |
32268 |
|
Bap60 |
DRSC32657 |
55.5 |
Decreased HIF dependent transcription with desferrioxamine |
|
yes |
group B |
HIF-dependent transcription (2) |
Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. |
Dekanty et al. |
2010 |
20585616 |
Cell line |
S2 |
Hypoxia inducible HRE reporter |
Luminescence |
DRSC 2.0 library |
Selected genes |
dsRNA |
Percentage |
Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA |
|
GR00170-A-2 |
32268 |
|
Bap60 |
DRSC32656 |
46.4 |
Decreased HIF dependent transcription with desferrioxamine |
|
yes |
group B |
HIF-dependent transcription (2) |
Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. |
Dekanty et al. |
2010 |
20585616 |
Cell line |
S2 |
Hypoxia inducible HRE reporter |
Luminescence |
DRSC 2.0 library |
Selected genes |
dsRNA |
Percentage |
Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA |
|
GR00170-A-3 |
32268 |
|
Bap60 |
DRSC32657 |
34.2 |
none |
|
no |
|
HIF-dependent transcription (3) |
Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. |
Dekanty et al. |
2010 |
20585616 |
Cell line |
S2 |
Hypoxia inducible HRE reporter |
Luminescence |
DRSC 2.0 library |
Selected genes |
dsRNA |
Percentage |
> |
|
GR00170-A-3 |
32268 |
|
Bap60 |
DRSC32656 |
16.1 |
none |
|
no |
|
HIF-dependent transcription (3) |
Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. |
Dekanty et al. |
2010 |
20585616 |
Cell line |
S2 |
Hypoxia inducible HRE reporter |
Luminescence |
DRSC 2.0 library |
Selected genes |
dsRNA |
Percentage |
> |
|
GR00183-A |
32268 |
FBgn0025463 |
Bap60 |
12675 |
sp |
GFP aggregates number, GFP aggregates size, lethal |
|
no |
|
Self-renewal and differentiation in neural stem cells |
Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. |
Neumueller et al. |
2011 |
21549331 |
Tissue |
insc-GAL4 |
Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability |
Fluorescence |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
> 1 |
Additional information about a secondary screen (KK library) |
GR00185-A |
32268 |
CG4303 |
Bap60 |
np |
142 |
none |
|
no |
20 min heat shock |
Pol II elongation |
Spt6 enhances the elongation rate of RNA polymerase II in vivo. |
Ardehali et al. |
2009 |
19279664 |
Cell line |
S2 |
Hsp70 mRNA expression |
Real-time qPCR |
Custom-made |
Transcription factors |
dsRNA |
Percentage |
< |
|
GR00190-A-1 |
32268 |
FBgn0025463 |
Bap60 |
12673 |
-0.692 |
none |
|
no |
|
Adiposity regulation (1) |
Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. |
Pospisilik et al. |
2010 |
20074523 |
Organism |
Hsp70-GAL4;Tub-GAL80ts |
Total fly triglyceride expression |
Colorimetrics |
np |
Genome-wide |
UAS-IR construct |
Triglyceride change |
Z-score > 1.65 after 3 screening rounds |
Additional information about the primary screen |
GR00191-A-1 |
32268 |
CG4303 |
Bap60 |
np |
np |
none |
|
yes |
|
Blood cell homeostasis regulation (1) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
srp-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
np |
np |
|
GR00191-A-2 |
32268 |
CG4303 |
Bap60 |
np |
np |
none |
|
no |
|
Blood cell homeostasis regulation (2) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
hml∆-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
np |
np |
|
GR00218-S |
32268 |
CG4303 |
Bap60 |
AMB18086 |
-0.11 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
32268 |
CG4303 |
|
4303R |
sp |
none |
|
no |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00273-A-1 |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
3 |
Effect on Polycomb (Pc) foci |
|
|
|
Polycomb group (PcG) proteins regulation (1) |
Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen |
Gonzalez et al. |
2014 |
24703951 |
Cell line |
S2 |
Pc-GFP protein localization |
Fluorescence |
DRSC |
Genome-wide and transcription factors |
dsRNA |
Visual inspection |
1 |
Additional phenotypic clustering screen available. |
GR00273-A-1 |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
3 |
Effect on Polycomb (Pc) foci |
|
|
|
Polycomb group (PcG) proteins regulation (1) |
Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen |
Gonzalez et al. |
2014 |
24703951 |
Cell line |
S2 |
Pc-GFP protein localization |
Fluorescence |
DRSC |
Genome-wide and transcription factors |
dsRNA |
Visual inspection |
1 |
Additional phenotypic clustering screen available. |
GR00273-A-2 |
32268 |
FBgn0025463 |
Bap60 |
DRSC19337 |
np |
Increased Polycomb (Pc) foci intensity; decreased Polycomb (Pc) foci intensity |
|
|
|
Polycomb group (PcG) proteins regulation (2) |
Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen |
Gonzalez et al. |
2014 |
24703951 |
Cell line |
S2 |
Polycomb-GFP protein localization |
Fluorescence |
DRSC |
Selected genes |
dsRNA |
Visual inspection |
np |
|
GR00282-A |
32268 |
CG4303 |
Bap60 |
12673 |
np |
none |
|
|
rudimentary ovaries |
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00282-A |
32268 |
CG4303 |
Bap60 |
12675 |
np |
none |
|
|
rudimentary ovaries |
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
32268 |
CG4303 |
Bap60 |
103634 |
-0.015251678455937875 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00305-A |
32268 |
FBgn0025463 |
Bap60 |
np |
1.758496123417408 |
Increased Rift Valley fever virus (RVFV) infection |
|
|
score in duplicate screen: 3.46 |
Rift Valley fever virus (RVFV) infection |
A genome-wide RNAi screen reveals that mRNA decapping restricts bunyaviral replication by limiting the pools of Dcp2-accessible targets for cap-snatching |
Hopkins et al. |
2013 |
23824541 |
Cell line |
DL1 |
RVFV N protein expression and DNA content |
Fluorescence |
Ambion |
Genome-wide |
dsRNA |
Z-score |
> |
Additional secondary screen available. RVFV strain MP12 used in both screens. |
GR00311-A |
32268 |
np |
np |
DRSC19337 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00315-A-1 |
32268 |
CG4303 |
Bap60 |
103634 |
np |
Effect on follicular epithelium morphology |
|
|
further phenotype data in classification screen |
Follicular epithelium development (1) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Random genes |
UAS-IR construct |
Visual inspection |
np |
Further classification screen available, see follicular epithelium development (2). |
GR00315-A-2 |
32268 |
CG4303 |
Bap60 |
103634 |
np |
Defect in formation phase |
|
|
|
Follicular epithelium development (2) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. |
GR00323-A-1 |
32268 |
FBgn0025463 |
Bap60 |
12675 |
np |
Lethal |
|
|
|
Drosophila eye development |
The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development |
Marinho et al. |
2013 |
23416177 |
Tissue |
eyeless-GAL4 |
Retina size |
Microscopy |
VDRC, NIG and TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
Single RNAi screen; For double RNAi screen see "Viriato (Vito) interactions during eye development |
GR00323-A-2 |
32268 |
FBgn0025463 |
Bap60 |
12675 |
np |
Lethal |
|
|
|
Viriato (Vito) interactions during eye development |
The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development |
Marinho et al. |
2013 |
23416177 |
Tissue |
eyeless-Gal4 |
Retina size |
Microscopy |
VDRC, NIG and TRiP |
Selected genes |
UAS-IR construct |
Genetic interaction score |
np |
Double RNAi screen; vito knockdown was archieved by expression of UAS-vitoRNAiKK in the female flies. For single RNAi screen see "Drosophila eye development |
GR00326-A |
32268 |
FBgn0037153 |
olf413 |
12673 |
np |
none |
|
|
|
Synapse formation and maintenance |
A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance |
Valakh et al. |
2012 |
22542760 |
Tissue |
Elav-Gal4 |
Neuromuscular junction morphology |
Fluorescence |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
For further phenotype explanations sp. |
GR00335-A |
32268 |
FBgn0025463 |
Bap60 |
HMS00507 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
GR00335-A |
32268 |
FBgn0025463 |
Bap60 |
HMS00909 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
GR00335-A |
32268 |
FBgn0025463 |
Bap60 |
JF01295 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
|
32268 |
FBgn0025463 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
32268 |
CG4303 |
|
12673 |
np |
liquid clearance defect |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00379-A-1 |
32268 |
CG4303 |
|
103634 |
-0.014624397332044185 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00388-A |
32268 |
CG4303 |
|
BL33954 |
|
Lethal |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
32268 |
CG4303 |
|
BL31337 |
|
none |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
32268 |
CG4303 |
|
BL32503 |
|
Lethal |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
32268 |
CG4303 |
|
GD12673 |
|
Lethal |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |