RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 32314
  • Symbol: up
  • Description: upheld
DataSource: http://genomernai.dkfz.de/v16/genedetails/32314

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 32314 FBgn0004169 up DRSC20382 0.336179468 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 32314 FBgn0004169 up DRSC20382 0.595816494 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 32314 FBgn0004169 up DRSC20382 -2.387411564 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 32314 FBgn0004169 up DRSC20382 1.68675915 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00004-A-0 32314 up DRSC20382 0.59 Endo-siRNA reporter downregulated no Drosophila small RNA pathways Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Zhou et al. 2008 19026789 Cell line S2 miRNA and siRNA pathway activity Dual luciferase DRSC dsRNA Fold change endo-siRNA reporter 1.5/0.6
GR00031-A-1 32314 HFA20382 1.2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 32314 HFA20382 0.5 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
32314 FBgn0004169 up LD45641 sp none no library: DGC1
32314 FBgn0015567, FBgn0004169 alpha-Adaptin, up LD08591 sp none no library: DGC1
GR00130-A 32314 FBgn0004169 up np <= -2 Decreased mutant human huntingtin aggregation no Mutant human huntingtin aggregation RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. Doumanis et al. 2009 19789644 Cell line BG2-c2 Nhtt(62Q)EGFP aggregate number and size Fluorescence OpenBiosystems RNAi library Selected genes dsRNA Z-score Suppressor: <
GR00135-A-1 32314 CG7107 up 27853 -1.17 Developmentally lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 32314 CG7107 27853 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 32314 CG7107 up 27853 -0.985294117647058 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 32314 CG7107 27853 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00148-A 32314 FBgn0004169 up np -2.35 Decreased P-JNK protein expression with PGN 15 min PGN induction no PGN-induced dJNK phosphorylation A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. Bond and Foley 2009 19893628 Cell line S2 P-JNK protein expression Fluorescence Custom-made Genome-wide dsRNA Z-score Complex criteria Only hits stored in GenomeRNAi
GR00159-A 32314 FBgn0004169 up DRSC20382, DRSC28798 np Muscles spread, actin and myosin filaments lack striation no Muscle assembly and maintenance RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Bai et al. 2008 18359903 Primary cells primary embryonic cells Muscle cell morphology Fluorescence np Selected and random genes dsRNA Percentage of muscles with a given phenotype Severe: > 80 %; medium: ~ 50 %
GR00190-A-1 32314 FBgn0004169 up 27853 0.02 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00238-S-1 32314 CG7107 troponin-T 27853 sp Embryo lethal yes library: VDRC Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2 32314 CG7107 troponin-T 27853 sp Climbing defect no library: VDRC Muscle maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue TARGET Mef2-GAL4 Climbing ability Negative geotaxis assay np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00284-A 32314 CG7107 up 27853 1.0089258164725226 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 32314 FBgn0004169 up DRSC20382 np Actin defect decreased level of actin Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A 32314 FBgn0004169 up JF01102 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 32314 FBgn0004169 up HMS00743 np F1 lethal F1 embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
32314 FBgn0004169 np np sp none
32314 np np HFA20382 0.15 none fold change: 0.94
GR00367-S 32314 CG7107 27853 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 32314 CG7107 27853 -0.29442724757186123 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 32314 CG7107 BL32949 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.