RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 32319
• Symbol: Tango2
• Description: Transport and Golgi organization 2

DataSource: http://genomernai.dkfz.de/v16/genedetails/32319

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	32319	FBgn0030503	CG11176	DRSC19394	-0.674999667	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32319	FBgn0030503	CG11176	DRSC19394	-0.287697533	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32319	FBgn0030503	CG11176	DRSC19394	0.156983246	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32319	FBgn0030503	CG11176	DRSC19394	-0.690901611	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32319	FBgn0030503	CG11176	DRSC21526	-0.197360371	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32319	FBgn0030503	CG11176	DRSC21526	-0.930401196	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	32319			HFA19394	-0.1	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	32319			HFA19394	-1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00047-A-1	32319	FBgn0030503	CG11176	DRSC19394	sp	Decreased horseradish peroxidase protein expression		no		Constitutive protein secretion and Golgi organization (1)	Functional genomics reveals genes involved in protein secretion and Golgi organization.	Bard et al.	2006	16452979	Cell line	S2	Horseradish peroxidase protein expression	Luminescence		Genome-wide	dsRNA	Z-score	< -1.5
GR00047-A-2	32319	FBgn0030503	CG11176	np	np	Fused Golgi membranes with endoplasmic reticulum		yes		Constitutive protein secretion and Golgi organization (2)	Functional genomics reveals genes involved in protein secretion and Golgi organization.	Bard et al.	2006	16452979	Cell line	S2	Mouse Mannosidase II protein expression	Fluorescence		Selected genes	dsRNA	Visual inspection	Golgi membrane depletion > 50 % of the cells
	32319	FBgn0030503	CG11176	LD02616	sp	none		no	library: DGC1
GR00134-A-1	32319	CG11176	Tango2	18175	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	32319	CG11176	Tango2	18175	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	32319	CG11176		18175	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00142-A-1	32319	CG11176	Tango2	18175	0.703703703703703	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	32319	CG11176		18175	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00189-A-1	32319	FBgn0030503	Tango2	BKN20133	np	Downregulation of Notch pathway		yes		Notch pathway regulation (1)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Cell line	S2	Notch pathway reporter	Luminescence	BKN	Genome-wide	dsRNA	Complex, sp	Complex criteria
GR00189-A-2	32319	FBgn0030503	Tango2	18175	strong	Adult wing posterior compartment: blister		yes	en-GAL4	Notch pathway regulation (2)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4, C96-GAL4 and vg-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-3	32319	FBgn0030503	Tango2	np	np	none		no		Notch pathway regulation (3)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	GMR-GAL4	Notch pathway reporter	Fluorescence	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Average fluorescent intensity	Complex criteria
GR00190-A-1	32319	FBgn0030503	Tango2	18175	-0.156	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00218-S	32319	CG11176		AMB34591	0.16	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	32319	CG11176	Tango2	18175	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	32319	CG11176	Tango2	18175	0.16714190102557175	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	32319	FBgn0030503	Tango2	DRSC19394	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
	32319	FBgn0030503	np	np	sp	none
GR00367-S	32319	CG11176		18175	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	32319	CG11176		18175	-0.39166205020578587	none			Intermediate eggs/larvae level	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2	32319	CG11176		18175	np	none				Defects in female germline	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	Vasa AND 1B1 expression	Immunofluorescence	VDRC	Selected genes	UAS-IR construct	np	np	Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	32319	CG11176		GD18175		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.