Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 32518
Symbol: CCT6
Description: Chaperonin containing TCP1 subunit 6

DataSource: http://genomernai.dkfz.de/v16/genedetails/32518

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	32518	FBgn0027329	l(1)G0022	DRSC20095	0.900133699	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32518	FBgn0027329	l(1)G0022	DRSC20095	-1.565873592	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32518	FBgn0027329	l(1)G0022	DRSC20095	0.454161165	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32518	FBgn0027329	l(1)G0022	DRSC20095	0.709855093	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0	32518	FBgn0027329		DRSC20095	weak	ERK activation increased		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	32518			HFA20095	0.3	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	32518			HFA20095	-0.7	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00047-A-1	32518	FBgn0027329	l(1)G0022	DRSC20095	sp	Decreased horseradish peroxidase protein expression		no		Constitutive protein secretion and Golgi organization (1)	Functional genomics reveals genes involved in protein secretion and Golgi organization.	Bard et al.	2006	16452979	Cell line	S2	Horseradish peroxidase protein expression	Luminescence		Genome-wide	dsRNA	Z-score	< -1.5
	32518	FBgn0027329	l(1)G0022	GH13725	sp	none		no	library: DGC1
GR00080-A-0	32518	FBgn0027329	CG8231	np	np	Centriole duplication defect and pericentriolar material (PCM) defect		no		Centriole duplication and centrosome maturation	A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.	Dobbelaere et al.	2008	18798690	Cell line	S2R+	Centrosome number	High content (microscopy)	Ambion		dsRNA	Visual inspection	np
GR00086-A-0	32518	FBgn0027329		DRSC20095	-0.7/-2.6	Decreased Influenza replication		no		Influenza replication	Drosophila RNAi screen identifies host genes important for influenza virus replication.	Hao et al.	2008	18615016	Cell line	DL1	Influenza replication	Luciferase	Ambion/DRSC		dsRNA	Z-score	<
GR00092-A	32518	FBgn0030681	CG8231	RDM1190-7301044	np	Monopolar spindle		no		Microtubule assembly and organization	Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin.	Ducat et al.	2008	18463163	Cell line	S2	DNA content, alpha-tubulin and CP309 protein expression	Fluorescence	OpenBiosystems Drosophila RNAi Library 1.0	Mitotic regulator genes	dsRNA	Complex, sp	Complex criteria
GR00134-A-1	32518	CG8231	Tcp-1zeta	23751	np	Lethal		no	late larval	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00142-A-1	32518	CG8231	Tcp-1zeta	23751	-0.6875	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	32518	CG8231		23751	sp	Completely lethal (adult)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00148-A	32518	FBgn0030681	l(1)G0022	np	2.23	Increased P-JNK protein expression with PGN	60 min PGN induction	no		PGN-induced dJNK phosphorylation	A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling.	Bond and Foley	2009	19893628	Cell line	S2	P-JNK protein expression	Fluorescence	Custom-made	Genome-wide	dsRNA	Z-score	Complex criteria	Only hits stored in GenomeRNAi
GR00172-A-1	32518		Tcp-1zeta, nbs	DRSC20095, custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		yes		G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00172-A-2	32518		Tcp-1zeta	DRSC20095	4.6	none		no	increased mitotic index without DNA damage	G2-M DNA damage checkpoint regulation (2)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Selected genes	dsRNA	Relative mitotic index	Complex criteria	Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00190-A-1	32518	FBgn0027329	Tcp-1zeta	23751	0.235	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00214-A-1	32518	FBgn0027329	Tcp-1zeta	DRSC20095	-0.28	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	32518	FBgn0027329	Tcp-1zeta	DRSC20095	0.97	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	32518	FBgn0027329	Tcp-1zeta	DRSC20095	-0.33	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	32518	FBgn0027329	Tcp-1zeta	DRSC20095	0.14	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	32518	CG8231		AMB20566	0.77	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	32518	CG8231	Tcp-1zeta	109734	np	none			rudimentary ovaries	piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	32518	CG8231	Tcp-1zeta	109734	-0.38623960473342567	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00308-A	32518	CG8231	CG8231	np	2	Decreased cell aggregation			validated in secondary screen	Cadherin-mediated cell-cell adhesion	A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cellâ€“cell adhesion	Toret et al.	2014	24446484	Cell line	S2	Cell aggregation	Fluorescence	V2 RNAi library (Thermo Fisher Scientific)	Genome-wide	dsRNA	Visual inspection; average of 3 replicates; 0	> 1.5	Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A	32518	FBgn0027329	Tcp-1zeta	DRSC20095	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	32518	CG8231	Tcp-1zeta	109734	np	Effect on follicular epithelium morphology			further phenotype data in classification screen	Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00315-A-2	32518	CG8231	Tcp-1zeta	109734	np	No ovaries				Follicular epithelium development (2)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Selected genes	UAS-IR construct	Visual inspection	np	For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
	32518	FBgn0027329	np	np	sp	none
GR00367-S	32518	CG8231		23751	np	liquid clearance defect			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	32518	CG8231		109734	2.8646695448229265	Medium decrease in nos and yTub37c expression			No eggs/larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2	32518	CG8231		109734	np	No germline, 100%				Defects in female germline	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	Vasa AND 1B1 expression	Immunofluorescence	VDRC	Selected genes	UAS-IR construct	np	np	Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	32518	CG8231		GD23751		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.