| GR00002-A |
32789 |
FBgn0021765 |
scu |
DRSC20369 |
0.564154441 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
32789 |
FBgn0021765 |
scu |
DRSC20369 |
0.117224658 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00013-A |
32789 |
CG7113 |
scu |
np |
np |
Decreased Chlamydia caviae infection |
|
yes |
|
Chlamydia caviae infection |
RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection. |
Derre et al. |
2007 |
17967059 |
Cell line |
SL2 |
Chlamydia MOMP and LPS expression and DNA content |
Fluorescence |
np |
Genome-wide |
dsRNA |
Visual inspection |
np |
Additional information about secondary screens |
GR00031-A-1 |
32789 |
|
|
HFA20369 |
0.8 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
32789 |
|
|
HFA20369 |
0.3 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
32789 |
FBgn0021765 |
scu |
RE18259 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
32789 |
FBgn0021765 |
scu |
RH43965 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00134-A-1 |
32789 |
CG7113 |
scu |
37083 |
np |
none |
|
no |
|
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00134-A-1 |
32789 |
CG7113 |
scu |
37085 |
np |
none |
|
no |
|
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00135-A-1 |
32789 |
CG7113 |
scu |
37083 |
-1.17 |
none |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-1 |
32789 |
CG7113 |
scu |
37085 |
-1.17 |
none |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00138-A-1 |
32789 |
CG7113 |
|
37085 |
1 |
none |
|
no |
|
Heart development and function (1) |
A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. |
Neely et al. |
2010 |
20371351 |
Tissue |
TinCΔ4 12a-Gal4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Developmental lethality |
< |
|
GR00139-A |
32789 |
|
scu |
37085 |
sp |
Whole fly: darker pigmentation, wings: dusky; semi-lethal |
|
no |
|
Fly viability and adult morphology |
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. |
Dietzl et al. |
2007 |
17625558 |
Organism |
Act5C-GAL4 |
Adult morphology and viability |
Visual inspection |
Custom-made |
Selected and random genes |
UAS-IR construct |
Phenotype strength |
np |
Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) |
GR00139-A |
32789 |
|
scu |
37083 |
sp |
Semi-lethal |
|
no |
|
Fly viability and adult morphology |
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. |
Dietzl et al. |
2007 |
17625558 |
Organism |
Act5C-GAL4 |
Adult morphology and viability |
Visual inspection |
Custom-made |
Selected and random genes |
UAS-IR construct |
Phenotype strength |
np |
Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) |
GR00142-A-1 |
32789 |
CG7113 |
scu |
37085 |
-2.43 |
Decreased viability after Serratia marcescens infection |
|
yes |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00142-A-2 |
32789 |
CG7113 |
scu |
np |
sp |
Decreased viability after Serratia marcescens infection |
|
yes |
|
Serratia marcescens infection (2) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Tissue |
HML-GAL4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Complex, sp |
Significantly reduced survival (p < 0.05) |
|
GR00142-A-4 |
32789 |
CG7113 |
scu |
np |
sp |
Decreased viability after Serratia marcescens infection |
|
no |
|
Serratia marcescens infection (4) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Tissue |
NP1-GAL4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Complex, sp |
Significantly reduced survival (p < 0.05) |
|
GR00144-A-4 |
32789 |
CG7113 |
|
37083 |
0 |
none |
|
no |
not lethal |
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-4 |
32789 |
CG7113 |
|
37085 |
0 |
none |
|
no |
not lethal |
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00218-S |
32789 |
CG7113 |
scu |
AMB20446 |
-2.51 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
32789 |
CG7113 |
|
7113R |
sp |
none |
|
no |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00282-A |
32789 |
CG7113 |
scu |
110802 |
np |
none |
|
|
|
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00282-A |
32789 |
CG7113 |
scu |
37083 |
np |
none |
|
|
|
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
32789 |
CG7113 |
scu |
37083 |
1.1022305265519472 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00311-A |
32789 |
FBgn0021765 |
scu |
DRSC20369 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
|
32789 |
FBgn0021765 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
32789 |
np |
np |
HFA20369 |
-1.96 |
none |
|
|
fold change: 2.42 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
32789 |
CG7113 |
|
37083 |
np |
lethal or adult morphology phenotype |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00379-A-1 |
32789 |
CG7113 |
|
37083 |
-0.5436471071905499 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00388-A |
32789 |
CG7113 |
|
GD37085 |
|
none |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |