Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 3355136
Symbol: Nipped-B
Description: Nipped-B

DataSource: http://genomernai.dkfz.de/v16/genedetails/3355136

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	3355136	FBgn0026401	Nipped-B	DRSC07815	-0.105794016	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	3355136	FBgn0026401	Nipped-B	DRSC07815	-0.159531401	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	3355136			HFA07815	0.2	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	3355136			HFA07815	1.1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	3355136		CG40281	LD04986	sp	none		no	library: DGC1
GR00189-A-1	3355136	FBgn0026401	Nipped-B	BKN33715	np	Downregulation of Notch pathway		no		Notch pathway regulation (1)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Cell line	S2	Notch pathway reporter	Luminescence	BKN	Genome-wide	dsRNA	Complex, sp	Complex criteria
GR00214-A-1	3355136	FBgn0026401	Nipped-B	DRSC07815	0.13	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	3355136	FBgn0026401	Nipped-B	DRSC07815	0.95	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	3355136	FBgn0026401	Nipped-B	DRSC07815	2.06	Increased ERK phosphorylation after sSpitz and EGF stimulation	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	3355136	FBgn0026401	Nipped-B	DRSC07815	0.95	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	3355136	CG40281		AMB29051	2.34	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	3355136	CG17704		17704R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00273-A-1	3355136	FBgn0026401	Nipped-B	DRSC07815	1	Effect on Polycomb (Pc) foci				Polycomb group (PcG) proteins regulation (1)	Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen	Gonzalez et al.	2014	24703951	Cell line	S2	Pc-GFP protein localization	Fluorescence	DRSC	Genome-wide and transcription factors	dsRNA	Visual inspection	1	Additional phenotypic clustering screen available.
GR00273-A-1	3355136	FBgn0026401	Nipped-B	DRSC29151	3	Effect on Polycomb (Pc) foci				Polycomb group (PcG) proteins regulation (1)	Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen	Gonzalez et al.	2014	24703951	Cell line	S2	Pc-GFP protein localization	Fluorescence	DRSC	Genome-wide and transcription factors	dsRNA	Visual inspection	1	Additional phenotypic clustering screen available.
GR00273-A-2	3355136	FBgn0026401	Nipped-B	DRSC29151	np	Decreased Polycomb (Pc) foci intensity				Polycomb group (PcG) proteins regulation (2)	Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen	Gonzalez et al.	2014	24703951	Cell line	S2	Polycomb-GFP protein localization	Fluorescence	DRSC	Selected genes	dsRNA	Visual inspection	np
GR00273-A-2	3355136	FBgn0026401	Nipped-B	DRSC07815	np	Decreased Polycomb (Pc) foci intensity				Polycomb group (PcG) proteins regulation (2)	Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen	Gonzalez et al.	2014	24703951	Cell line	S2	Polycomb-GFP protein localization	Fluorescence	DRSC	Selected genes	dsRNA	Visual inspection	np
GR00311-A	3355136	FBgn0026401	Nipped-B	DRSC07815	np	Other cellular phenotype			increased cell size, increased number of multinucleate cells, decreased cell number, loss of cell monolayer	Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	3355136	FBgn0026401	Nipped-B	GL00574	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	3355136	FBgn0026401	Nipped-B	HMS00401	np	Oogenesis defect				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	3355136	FBgn0026401	Nipped-B	HM05172	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	3355136	FBgn0026401	np	np	sp	none
GR00388-A	3355136	CG17704		BL28961		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	3355136	CG17704		BL32406		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.