RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 33834
  • Symbol: chic
  • Description: chickadee
DataSource: http://genomernai.dkfz.de/v16/genedetails/33834

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 33834 FBgn0000308 chic DRSC03507 -0.058579854 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 33834 FBgn0000308 chic DRSC03507 -0.391963163 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00029-A-0 33834 FBgn0000308 chic np np Cytokinesis failure no Actin morphology Molecular requirements for actin-based lamella formation in Drosophila S2 cells. Rogers et al. 2003 12975351 Cell line S2 Actin morphology High content (microscopy) Custom-made dsRNA Visual inspection np
GR00031-A-1 33834 HFA03507 1.5 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 33834 HFA03507 0.1 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00032-A-0 33834 FBgn0000308 64529 np Multi-nucleate cells no Cytokinesis Terminal cytokinesis events uncovered after an RNAi screen. Echard et al. 2004 15380073 Cell line S2 Multinucleate cell incidence High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00036-A 33834 CG9553 chic DRSC03507 moderate Decreased GFP protein expression yes decreased intracellular infection Intracellular Listeria monocytogenes infection Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Agaisse et al. 2005 16020693 Cell line SL2 GFP protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np
GR00046-A-1 33834 FBgn0000308 Chic DRSC03507 2 Decreased M. fortuitum intracellular infection measurement after 3 days no Mycobacterial infection (1) - M. fortuitum infection Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection. Philips et al. 2005 16020694 Cell line S2 map24::GFP and map49::GFP protein expression Fluorescence np Genome-wide dsRNA Z-score >
33834 FBgn0000308 chic LD15851 sp none no library: DGC1
33834 FBgn0000308 chic LD19369 sp none no library: DGC2
GR00084-A-0 33834 FBgn0000308 CG9553 np np C. trachomatis infection down no C. trachomatis infection RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry. Elwell et al. 2008 18369471 Cell line S2 C. trachomatis infection High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00130-A 33834 FBgn0000308 chic np <= -2 Decreased mutant human huntingtin aggregation no Mutant human huntingtin aggregation RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. Doumanis et al. 2009 19789644 Cell line BG2-c2 Nhtt(62Q)EGFP aggregate number and size Fluorescence OpenBiosystems RNAi library Selected genes dsRNA Z-score Suppressor: <
GR00134-A-1 33834 CG9553 chic 15918 np Lethal yes embryonic Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-2 33834 CG9553 chic np np Wing posture / locomotion / ability to fly defect or lethal / semi-lethal no Muscle morphogenesis and function (2) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection Custom-made Selected genes UAS-IR construct rp S19 > 0.5
GR00142-A-1 33834 CG9553 chic 15918 -2.23711340206185 Decreased viability after Serratia marcescens infection no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00194-A 33834 CG9553 Chic, Profilin np sp Increased actin protein expression at cell perimeter on Con A surface, lack of cell spreading on Con A surface yes actin perimeter: bottom 40, actin perimeter-1: top 40 S2 cell spreading A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. D'Ambrosio and Vale 2010 21041442 Cell line S2U alpha-tubulin and actin protein expression Fluorescence V2 RNAi library Genome-wide dsRNA Complex, sp Complex criteria
GR00218-S 33834 CG9553 chic AMB34945 0.09 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00238-S-1 33834 CG9553 chic 9553R-3 sp Pupal lethal yes library: NIG Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-1 33834 CG9553 chic 102759 sp Embryo lethal yes library: VDRC Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2 33834 CG3937 cher 102759 sp Climbing defect no library: VDRC Muscle maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue TARGET Mef2-GAL4 Climbing ability Negative geotaxis assay np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2 33834 CG9553 chic 9553R-3 sp Climbing defect no library: NIG Muscle maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue TARGET Mef2-GAL4 Climbing ability Negative geotaxis assay np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2 33834 CG9553 chic 102759 sp none no library: VDRC Muscle maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue TARGET Mef2-GAL4 Climbing ability Negative geotaxis assay np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1 33834 CG9553 9553R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 33834 CG9553 chic 102759 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 33834 CG9553 chic 102759 0.41897559611391155 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00308-A 33834 CG9553 chic np 3 Decreased cell aggregation validated in secondary screen Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 33834 FBgn0000308 chic DRSC03507 np Actin defect, microtubule defect, other cellular phenotype decreased cell size, cell shape round or non-adherent, decreased level of actin, increased nuclear actin, microtubule processes, loss of cell monolayer Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 33834 CG9553 chic 102759 np Effect on follicular epithelium morphology further phenotype data in classification screen Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00315-A-2 33834 CG9553 chic 102759 np No ovaries Follicular epithelium development (2) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Selected genes UAS-IR construct Visual inspection np For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00335-A 33834 FBgn0000308 chic HMS00550 np F1 lethal F1 larval and pupal lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
33834 FBgn0000308 np np sp none
GR00367-S 33834 CG9553 102759 np liquid clearance defect VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 33834 CG9553 102759 -0.27526963976698776 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 33834 CG9553 BL34523 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 33834 CG9553 KK102759 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.