RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 33916
• Symbol: eya
• Description: eyes absent

DataSource: http://genomernai.dkfz.de/v16/genedetails/33916

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	33916	FBgn0000320	eya	DRSC03510	1.063662742	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	33916	FBgn0000320	eya	DRSC03510	0.066508627	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	33916			HFA03510	-0.2	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	33916			HFA03510	1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	33916	FBgn0000320	eya	GH05272	sp	none		no	library: DGC1
GR00130-A	33916	FBgn0000320	eya	np	1.5 - 2	Increased mutant human huntingtin aggregation		no		Mutant human huntingtin aggregation	RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation.	Doumanis et al.	2009	19789644	Cell line	BG2-c2	Nhtt(62Q)EGFP aggregate number and size	Fluorescence	OpenBiosystems RNAi library	Selected genes	dsRNA	Z-score	Suppressor: <
GR00135-A-1	33916	CG9554	eya	43911	1.22	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00142-A-1	33916	CG9554	eya	43911	-0.0309278350515463	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00142-A-1	33916	CG9554	eya	43911	0.074074074074074	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	33916	CG9554		43911	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00189-A-1	33916	FBgn0000320	eya	BKN27568	np	Upregulation of Notch pathway		no		Notch pathway regulation (1)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Cell line	S2	Notch pathway reporter	Luminescence	BKN	Genome-wide	dsRNA	Complex, sp	Complex criteria
GR00190-A-1	33916	FBgn0000320	eya	43911	0.283	Increased triglyceride expression		yes		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00190-A-2	33916	FBgn0000320	eya	43911	d/l	Lethal or developmental delay		no		Adiposity regulation (2)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	nsyb-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-3	33916	FBgn0000320	eya	43911	0.61	Increased triglyceride expression		no		Adiposity regulation (3)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	C57-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-4	33916	FBgn0000320	eya	43911	0.001	none		no		Adiposity regulation (4)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	oe-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-5	33916	FBgn0000320	eya	43911	-0.809	Decreased triglyceride expression		no		Adiposity regulation (5)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	ppl-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00218-S	33916	CG9554	eya	AMB22393	1.19	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	33916	CG9554	eya	43911	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	33916	CG9554	eya	108071	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	33916	CG9554	eya	108071	0.9888564629894478	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	33916	np	np	DRSC03510	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	33916	CG9554	eya	108071	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00323-A-1	33916	FBgn0000320	eya	43911	np	Decreased retina size				Drosophila eye development	The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development	Marinho et al.	2013	23416177	Tissue	eyeless-GAL4	Retina size	Microscopy	VDRC, NIG and TRiP	Selected genes	UAS-IR construct	Visual inspection	np	Single RNAi screen; For double RNAi screen see "Viriato (Vito) interactions during eye development
GR00323-A-2	33916	FBgn0000320	eya	43911	np	Strong vito interaction				Viriato (Vito) interactions during eye development	The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development	Marinho et al.	2013	23416177	Tissue	eyeless-Gal4	Retina size	Microscopy	VDRC, NIG and TRiP	Selected genes	UAS-IR construct	Genetic interaction score	np	Double RNAi screen; vito knockdown was archieved by expression of UAS-vitoRNAiKK in the female flies. For single RNAi screen see "Drosophila eye development
GR00326-A	33916	FBgn0000320	eya	108071	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00326-A	33916	FBgn0000320	eya	9554	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00335-A	33916	FBgn0000320	eya	JF03160	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	33916	FBgn0000320	np	np	sp	none
GR00367-S	33916	CG9554		43911	np	lethal or adult morphology phenotype			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	33916	CG9554		108071	-0.31860012157102147	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	33916	CG9554		BL28733		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	33916	CG9554		KK108071		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.