RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 34329
  • Symbol: RpL13
  • Description: Ribosomal protein L13
DataSource: http://genomernai.dkfz.de/v16/genedetails/34329

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 34329 FBgn0011272 RpL13 DRSC03416 4.008611361 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 34329 FBgn0011272 RpL13 DRSC03416 2.842067658 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1 34329 HFA03416 2.3 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 34329 HFA03416 2 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00036-A 34329 CG4651 RpL13 np moderate Increased GFP protein expression no increased intracellular infection Intracellular Listeria monocytogenes infection Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Agaisse et al. 2005 16020693 Cell line SL2 GFP protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np
GR00038-A-0 34329 FBgn0011272 RpL13 np * Less vacuolar escape no L. monocytogenes infection Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. Cheng et al. 2005 16157870 Cell line S2 L. monocytogenes infection High content (microscopy) Custom-made dsRNA Visual inspection np
GR00039-A-0 34329 FBgn0011272 DRSC03416 np Drosophila C virus infection reduced no Drosophila C virus infection Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition. Cherry et al. 2005 15713840 Cell line S2 Drosophila C virus infection High content (microscopy) DRSC dsRNA Visual inspection > 40% reduced infection
GR00045-A-0 34329 FBgn0011272 DRSC03416 -2.15 Decreased Hedgehog reporter activity no Hedgehog signaling A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken et al. 2005 16311596 Cell line Clone 8 Hedgehog signaling Dual luciferase DRSC dsRNA Z-score >
GR00047-A-1 34329 FBgn0011272 RpL13 DRSC03416 sp Decreased horseradish peroxidase protein expression no Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
34329 FBgn0011272 RpL13 GH08645 sp Increased G1 DNA content, decreased G2 DNA content, decreased viability yes library: DGC2
GR00048-A-2 34329 FBgn0011272 RpL13 np 0.652 Decreased viability yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 34329 FBgn0011272 RpL13 np 425 none no Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00051-A-1 34329 CG4651 RpL13 np np none no Candida albicans phagocytosis (1) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 C. albicans phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing C. albicans <
GR00086-A-0 34329 FBgn0011272 DRSC03416 -0.75/-3.49 Decreased Influenza replication no Influenza replication Drosophila RNAi screen identifies host genes important for influenza virus replication. Hao et al. 2008 18615016 Cell line DL1 Influenza replication Luciferase Ambion/DRSC dsRNA Z-score <
34329 FBgn0011272 RpL13 np 1.349 Increased mitochondrial citrate synthase activity, decreased viability no Renilla activity < 50% of mean
GR00090-A-0 34329 FBgn0011272 CG4651 np strong (but ribosomal) More dispersed droplets no Lipid storage Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Guo et al. 2008 18408709 Cell line S2 Lipid droplet staining High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00128-A-2 34329 FBgn0011272 CG4651 DRSC32541 sp none no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00137-A-1 34329 CG4651 np 1.4 none no decreased cell number or influenced reporter expression Regulated secretory pathway biogenesis (1) RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway. Asensio et al. 2010 21149569 Cell line S2 dVMAT protein cell surface expression Flow cytometry DmRNAi library version 1 Selected genes dsRNA Z-score >
GR00137-A-1 34329 CG4651 np 0.7 none no decreased cell number or influenced reporter expression Regulated secretory pathway biogenesis (1) RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway. Asensio et al. 2010 21149569 Cell line S2 dVMAT protein cell surface expression Flow cytometry DmRNAi library version 1 Selected genes dsRNA Z-score >
GR00170-A-1 34329 RpL13 DRSC03416 -6.4 none no decreased cell viability or Sanger collection sequence HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00175-A 34329 CG4651 RpL13 DRSC32541 -0.294164203833156 Downregulation of Hh pathway no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00189-A-1 34329 FBgn0011272 RpL13 BKN28033 np Downregulation of Notch pathway no Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00214-A-1 34329 FBgn0011272 RpL13 DRSC03416 -4.39 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 34329 FBgn0011272 RpL13 DRSC03416 -1.22 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 34329 FBgn0011272 RpL13 DRSC03416 1.65 Increased ERK phosphorylation after sSpitz and EGF stimulation 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 34329 FBgn0011272 RpL13 DRSC03416 6.38 Increased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 34329 CG4651 RpL13 AMB22712 -1.49 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 34329 CG4651 4651R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00273-A-1 34329 FBgn0011272 RpL13 DRSC03416 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00282-A 34329 CG4651 RpL13 109503 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 34329 CG4651 RpL13 109503 -1.0144676763164855 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00291-A 34329 RpL13 DRSC03416 sp none eliminated based on control score Hedgehog (hh) secretion regulation A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila Aikin et al. 2012 22432040 Cell line S2 Hedgehog protein expression Luminescence DRSC Genome-wide dsRNA Z-score < -2 OR > 2 by at least 1/3 Z-scores 296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available.
GR00305-A 34329 FBgn0011272 RpL13 np 1.4851924474490055 Increased Rift Valley fever virus (RVFV) infection score in duplicate screen: 3.92 Rift Valley fever virus (RVFV) infection A genome-wide RNAi screen reveals that mRNA decapping restricts bunyaviral replication by limiting the pools of Dcp2-accessible targets for cap-snatching Hopkins et al. 2013 23824541 Cell line DL1 RVFV N protein expression and DNA content Fluorescence Ambion Genome-wide dsRNA Z-score > Additional secondary screen available. RVFV strain MP12 used in both screens.
GR00308-A 34329 CG4651 RpL13 np 2.33 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 34329 FBgn0011272 RpL13 DRSC03416 np Actin defect, other cellular phenotype cell shape variable, decreased level of actin, decreased cell number, loss of cell monolayer Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 34329 CG4651 RpL13 109503 np Effect on follicular epithelium morphology further phenotype data in classification screen Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00315-A-2 34329 CG4651 RpL13 109503 np No ovaries Follicular epithelium development (2) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Selected genes UAS-IR construct Visual inspection np For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
34329 FBgn0011272 np np sp Increased transposon expression
GR00339-A-2 34329 FBgn0011272 RpL13 109503 na Developmental defect piRNA pathway regulation (2) A Genome-wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary piRNA Biogenesis in Drosophila Muerdter et al. 2013 23665228 Tissue traffic jam GAL4 Gypsy and ZAM transposon expression qPCR VDRC (GD, KK) and TRiP Selected genes UAS-IR construct Fold change > Following transposons were used for scoring: Gypsy for KK and ZAM for GD RNAi lines. TRIP reagents als TRIP_11111 dargestellt, Suchbarkeit? Developmental defect: wurden nicht gemessen, passt np als Score?
GR00379-A-1 34329 CG4651 109503 4.450267122702362 High decrease in nos and yTub37c expression No eggs/larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2 34329 CG4651 109503 np No germline, 100% Defects in female germline Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 Vasa AND 1B1 expression Immunofluorescence VDRC Selected genes UAS-IR construct np np Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.