RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 34481
  • Symbol: Nup107
  • Description: Nucleoporin 107kD
DataSource: http://genomernai.dkfz.de/v16/genedetails/34481

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 34481 FBgn0027868 Nup170 DRSC01999 -0.026632376 none yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 34481 FBgn0027868 Nup170 DRSC01999 0.445163972 none yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 34481 FBgn0027868 DRSC01999 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 34481 HFA01999 2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 34481 HFA01999 1 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00034-A 34481 CG6743 Nup170 np 13 Increased Dipt::lacZ protein expression without lipopolysaccharide stimulation, CDRi Imd pathway regulation Functional dissection of an innate immune response by a genome-wide RNAi screen. Foley and O'Farrell 2004 15221030 Cell line S2 Dipt::lacZ protein expression Colorimetrics Custom-made Selected genes dsRNA Percentage LacZ-positive cells Complex criteria Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish)
GR00035-A 34481 CG6743 Nup170 KP107 sp none no Alternative splicing Identification of alternative splicing regulators by RNA interference in Drosophila. Park et al. 2004 15492211 Cell line S2 dAdar, para and Dscam mRNA expression Reverse transcription PCR and autoradiography Custom-made RNA-binding protein genes and spliceosomal genes dsRNA np Alternative exon splicing for >
GR00047-A-1 34481 FBgn0027868 Nup170 DRSC01999 sp Decreased horseradish peroxidase protein expression no Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
34481 FBgn0027868 Nup170 LD18761 sp none no library: DGC1
GR00062-A 34481 FBgn0027868 Nup170 np 2 Decreased Ca2+ influx Ca2+ channel regulation CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Vig et al. 2006 16645049 Cell line S2R+ Ca2+ influx Fluorescence np Genome-wide dsRNA Complex, sp >
GR00092-A 34481 FBgn0027868 CG6743 RDM1190-7296326 np none no Microtubule assembly and organization Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. Ducat et al. 2008 18463163 Cell line S2 DNA content, alpha-tubulin and CP309 protein expression Fluorescence OpenBiosystems Drosophila RNAi Library 1.0 Mitotic regulator genes dsRNA Complex, sp Complex criteria
GR00131-A-1 34481 Nup107 DRSC01999 50 - 75 Altered mitochondrial Ca2+ and/or H+ levels yes Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00131-A-2 34481 Nup107 DRSC01999, DRSC34579 50 - 75 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (2) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Selected genes dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00134-A-1 34481 CG6743 Nup107 22405 np Lethal yes late larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 34481 CG6743 Nup107 22407 np Lethal yes late larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-3 34481 CG6743 Nup107 22407 np none no Muscle morphogenesis and function (3) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 ZCL0663 protein expression Fluorescence np Selected genes UAS-IR construct np np
GR00135-A-1 34481 CG6743 Nup107 22407 -0.26 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 34481 CG6743 22407 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 34481 CG6743 Nup107 22407 1.45588235294117 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 34481 CG6743 22407 sp Completely lethal (before pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00190-A-1 34481 FBgn0027868 Nup107 22407 -0.095 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00214-A-1 34481 FBgn0027868 Nup107 DRSC01999 -0.71 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 34481 FBgn0027868 Nup107 DRSC01999 1.46 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 34481 FBgn0027868 Nup107 DRSC01999 -0.05 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 34481 FBgn0027868 Nup107 DRSC01999 -0.77 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00216-A 34481 FBgn0027868 Nup107 BKN28047 0.1 none no Wnt proteins secretion p24 proteins are required for secretion of Wnt ligands. Buechling et al. 2011 22094269 Cell line S2R+ Wg pathway reporter Luminescence BKN Cellular transporting machinery genes dsRNA Z-score > 2.5 OR < -2.5 Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S 34481 CG6743 AMB30498 -0.87 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00245-A 34481 FBgn0027868 Nup170 DRSC01999 np Decreased formation of mutant Huntingtin (Httex1-Qn) protein aggregates yes validated Huntingtin (Htt) aggregates formation A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila. Zhang et al. 2010 20100940 Cell line S2 Mutant Httex1-Qn-eGFP protein expression and DNA content Fluorescence Version 1 and np Genome-wide dsRNA Aggregate number, size, and signal intensity > 2 standard deviations Additional information about secondary screens (reproducibility and non-overlaping dsRNAs)
GR00282-A 34481 CG6743 Nup107 110759 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 34481 CG6743 Nup107 22407 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 34481 CG6743 Nup107 110759 -1.1316115729642302 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 34481 FBgn0027868 Nup107 DRSC01999 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00314-A 34481 FBgn0027868 Nup107 RNA002896 68.08 Decreased MAPK phosphorylation RAS-related MAPK activation A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila Ashton-Beaucage et al. 2014 24643257 Cell line S2 MAPK phosphorylation Fluorescence Custom-made Genome-wide dsRNA pMAPK signal (%GFP dsRNA) np S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai
34481 FBgn0027868 np np sp none
GR00367-S 34481 CG6743 22407 np liquid clearance defect VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 34481 CG6743 110759 -0.30270527313737294 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 34481 CG6743 GD22407 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.