Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 35532
Symbol: Tbce
Description: Tubulin-binding cofactor E

DataSource: http://genomernai.dkfz.de/v16/genedetails/35532

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	35532	FBgn0033055	CG7861	DRSC04897	0.734512071	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	35532	FBgn0033055	CG7861	DRSC04897	-0.440042638	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	35532			HFA04897	-1	none	yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	35532			HFA04897	0.4	none	no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	35532	FBgn0033055	CG7861	GM13256	sp	Decreased G1 DNA content	yes	library: DGC1
GR00048-A-2	35532	FBgn0033055	CG7861	np	1.288	none	yes		Cell size and cell-cycle regulation (2)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Proliferation and viability	Colorimetrics		Selected genes	dsRNA	MTS metabolic activity	> 2.5 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3	35532	FBgn0033055	CG7861	np	449	none	yes		Cell size and cell-cycle regulation (3)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Cell number	Fluorescence		Selected genes	dsRNA	Average cell number per field	> 2 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-4	35532	FBgn0033055	CG7861	np	below threshold	none	no		Cell size and cell-cycle regulation (4)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Number of mitotic cells	Fluorescence		Selected genes	dsRNA	Percentage	Visual inspection	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00073-A-0	35532	FBgn0033055	CG7861	np	np	Dim microtubules	no		Mitosis	Genes required for mitotic spindle assembly in Drosophila S2 cells.	Goshima et al.	2007	17412918	Cell line	S2	Mitotic Spindle Assembly	High content (microscopy)	OpenBiosystems		dsRNA	Visual inspection	np
GR00090-A-0	35532	FBgn0033055	CG7861	np	moderate	Smaller sized lipid droplets, more dispersed	no		Lipid storage	Functional genomic screen reveals genes involved in lipid-droplet formation and utilization.	Guo et al.	2008	18408709	Cell line	S2	Lipid droplet staining	High content (microscopy)	OpenBiosystems		dsRNA	Visual inspection	np
GR00138-A-1	35532	CG7861		34388	0.5	Embryonic lethal or no offspring	no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCÎ”4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00183-A	35532	FBgn0033055	CG7861	34388	sp	GFP aggregates number, GFP aggregates size, neuroblast larger cell size, neuroblast less large cells, neuroblast cell shape, ganglion mother cell shorter lineages, neuroblast loss, neuroblast loss with larger neuroblast cell size, neuroblast underprolifer	no		Self-renewal and differentiation in neural stem cells	Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi.	Neumueller et al.	2011	21549331	Tissue	insc-GAL4	Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability	Fluorescence	np	Genome-wide	UAS-IR construct	Phenotype strength	> 1	Additional information about a secondary screen (KK library)
GR00218-S	35532	CG7861		AMB30551	-1.98	none	no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	35532	CG7861		7861R	sp	Increased alpha1,6-fucose Chp glycosylation, increased mannose Chp glycosylation	yes		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00239-A-2	35532	CG7861		7861R	np	none	no		Glycosylation regulation (2)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation	Luminescence	np	Selected genes	UAS-IR construct	p-value	< 0.005
GR00282-A	35532	CG7861	tbce	105246	np	none			piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	35532	CG7861	tbce	34388	np	none			piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	35532	CG7861	tbce	105246	-0.37284988525182144	none			piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	35532	FBgn0033055	CG7861	DRSC04897	np	none			Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	35532	CG7861	tbce	105246	np	Effect on follicular epithelium morphology			Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00315-A-2	35532	CG7861	tbce	105246	np	none			Follicular epithelium development (2)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Selected genes	UAS-IR construct	Visual inspection	np	For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
	35532	FBgn0033055	np	np	sp	none
GR00367-S	35532	CG7861		34388	np	liquid clearance defect		VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	35532	CG7861		105246	0.12076655763910812	none		No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	35532	CG7861		GD34388		Lethal			Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.