RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 35697
  • Symbol: HisT
  • Description: Histamine transporter
DataSource: http://genomernai.dkfz.de/v16/genedetails/35697

Export (tab separated) Export to Excel
Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 35697 FBgn0033196 CG1358 DRSC05051 0.76874586 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 35697 FBgn0033196 CG1358 DRSC05051 -0.664418451 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 35697 FBgn0033196 CG1358 DRSC06405 2.763398986 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00012-A-1 35697 CG1358 CG1358 np np Increased viability with doxorubicin yes Caspase activation in apoptosis (1) - combinatorial effect with doxorubicin A genome-wide RNAi screen reveals multiple regulators of caspase activation. Yi et al. 2007 17998402 Cell line Kc167 Viability (synthetic lethal) Luminescence HFA Genome-wide dsRNA Z-score Complex criteria
GR00012-A-2 35697 CG1358 CG1358 np np none yes Caspase activation in apoptosis (2) - combinatorial effect with doxorubicin A genome-wide RNAi screen reveals multiple regulators of caspase activation. Yi et al. 2007 17998402 Cell line Kc167 Caspase 3/7 activity Luminescence HFA Selected genes dsRNA p-value < 0.05
GR00012-A-3 35697 CG12284, CG1358 diap1, CG1358 np np Increased viability with diap1 RNAi no Caspase activation in apoptosis (3) - combinatorial effect with diap1 RNAi A genome-wide RNAi screen reveals multiple regulators of caspase activation. Yi et al. 2007 17998402 Cell line Kc167 Viability (synthetic lethal) Luminescence HFA Selected genes dsRNA p-value < 0.05
GR00014-A 35697 CG1358 DRSC05052 strong Decreased Flag-Mad nuclear accumulation no Dpp pathway regulation Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. Xu et al. 2007 17785517 Cell line S2R+ Flag-Mad protein expression and subcellular location Fluorescence rp Genome-wide dsRNA Visual inspection np
GR00031-A-1 35697 HFA06405 0.3 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-1 35697 HFA05051 0.4 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 35697 HFA05051 0.3 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 35697 HFA06405 1.4 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
35697 FBgn0033196 CG1358 GH15861 sp none no library: DGC1
GR00135-A-1 35697 CG1358 CG1358 13313 0.07 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00142-A-1 35697 CG1358 CG1358 13313 -0.258333333333333 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 35697 CG1358 13313 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00169-A 35697 FBgn0033196 CG1358 DRSC06405 -2.5 none 10 min insulin stimulation no Akt-TOR pathway negative feedback regulation Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. Kockel et al. 2010 20585550 Cell line Kc167 dAkt phosphorylation Fluorescence Custom-made Genome-wide dsRNA Z-score < -2.5 OR > 2.5
GR00189-A-1 35697 FBgn0033196 CG1358 BKN29206 np Upregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 35697 FBgn0033196 CG1358 13313 sp Adult wing veins: disrupted; adult wing posterior compartment: smaller, crumbled yes en-GAL4; final gene scoring: downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 35697 FBgn0033196 CG1358 13313 sp Downregulation of Notch pathway; adult wing margin: notches yes C96-GAL4 Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 35697 FBgn0033196 CG1358 np np none no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00190-A-1 35697 FBgn0033196 CG1358 13313 0.089 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00191-A-1 35697 CG1358 CG1358 np np Increased melanotic mass formation yes Blood cell homeostasis regulation (1) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-2 35697 CG1358 CG1358 np np none yes Blood cell homeostasis regulation (2) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-3 35697 CG1358 CG1358 np 0 none yes Blood cell homeostasis regulation (3) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-4 35697 CG1358 CG1358 np 0 none yes Blood cell homeostasis regulation (4) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-5 35697 CG1358 CG1358 np 0 none no Blood cell homeostasis regulation (5) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00214-A-1 35697 FBgn0033196 CG1358 DRSC06405 -2.18 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 35697 FBgn0033196 CG1358 DRSC06405 -0.31 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 35697 FBgn0033196 CG1358 DRSC06405 0.1 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 35697 FBgn0033196 CG1358 DRSC06405 0.54 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00217-A-1 35697 CG1358 CG1358 np weak Decreased WgRluc and not changed sFluc protein expression yes Wg pathway regulation (1) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Cell line S2R+ WgRluc and sFluc protein expression Luminescence np Genome-wide dsRNA Phenotype strength Complex criteria Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-2 35697 CG1358 CG1358 13313 0 none yes Wg pathway regulation (2) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue ey-GAL4 GMR–GAL4 Viability and Wg protein expression Visual inspection and eye morphology KK Selected genes UAS-IR construct Phenotype strength np Phenotype strength: strong enhancement: 2; strong suppression: -2; unchanged: 0; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-2 35697 CG1358 CG1358 101453 np none yes Wg pathway regulation (2) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue ey-GAL4 GMR–GAL4 Viability and Wg protein expression Visual inspection and eye morphology KK Selected genes UAS-IR construct Phenotype strength np Phenotype strength: strong enhancement: 2; strong suppression: -2; unchanged: 0; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-3 35697 CG1358 CG1358 101453 stereotypic phenotype Wings: unfolded yes Wg pathway regulation (3) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue nub-GAL4 Viability and wing morphology Visual inspection KK Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-3 35697 CG1358 CG1358 13313 wildtype none yes Wg pathway regulation (3) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue nub-GAL4 Viability and wing morphology Visual inspection KK Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-4 35697 CG1358 CG1358 101453 stereotypic phenotype Wings: round no Wg pathway regulation (4) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue hhGAL4 Viability and wing morphology Visual inspection KK and np Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-4 35697 CG1358 CG1358 13313 wildtype none no Wg pathway regulation (4) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue hhGAL4 Viability and wing morphology Visual inspection KK and np Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00218-S 35697 CG1358 AMB23185 -1.64 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 35697 CG1358 1358R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00284-A 35697 CG1358 CG1358 101453 -0.06554300735363178 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 35697 FBgn0033196 CG1358 DRSC06405 np Actin defect, microtubule defect, other cellular phenotype cell shape variable, decreased level of actin, microtubule clumps Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00311-A 35697 FBgn0033196 CG1358 DRSC05051 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 35697 CG1358 CG1358 101453 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00326-A 35697 FBgn0033196 CG1358 1358 np none Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
35697 FBgn0033196 np np sp none
35697 np np HFA06405 -0.98 none fold change: 2.2
GR00367-S 35697 CG1358 101453 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 35697 CG1358 101453 -0.4713608346930799 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 35697 CG1358 KK101453 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.