| GR00002-A |
35847 |
FBgn0022027 |
l(2)44Db |
DRSC06486 |
1.788716696 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
35847 |
FBgn0022027 |
l(2)44Db |
DRSC06486 |
-0.371056992 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00014-A |
35847 |
|
Vps25 |
DRSC06486 |
weak |
Decreased Flag-Mad nuclear accumulation |
|
no |
|
Dpp pathway regulation |
Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. |
Xu et al. |
2007 |
17785517 |
Cell line |
S2R+ |
Flag-Mad protein expression and subcellular location |
Fluorescence |
rp |
Genome-wide |
dsRNA |
Visual inspection |
np |
|
GR00031-A-1 |
35847 |
|
|
HFA06486 |
-1.9 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
35847 |
|
|
HFA06486 |
-1.4 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
35847 |
FBgn0022027 |
l(2)44Db |
RH14578 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00069-A-0 |
35847 |
FBgn0022027 |
Vps25 |
np |
np |
No effect on M. smegmatis infection |
|
no |
|
M. smegmatis infection |
ESCRT factors restrict mycobacterial growth. |
Philips et al. |
2008 |
18287038 |
Cell line |
S2 |
M. smegmatis infection |
High content (microscopy) |
DRSC |
|
dsRNA |
np |
np |
|
GR00076-A |
35847 |
CG14750 |
Vps25 |
np |
4.09 |
none |
|
no |
|
CD4 regulation by human immunodeficiency virus Type 1 Nef (HIV-1 Nef) |
Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor. |
Chaudhuri et al. |
2007 |
17267500 |
Cell line |
S2 |
Human CD4 surface protein expression |
Fluorescence |
Expression Arrest Drosophila RNAi library |
Protein trafficking machinery genes |
dsRNA |
Fold downregulation |
np |
|
GR00128-A-1 |
35847 |
FBgn0022027 |
CG14750 |
DRSC06486 |
sp |
Synthetic lethal with methyl methanesulphonate |
|
no |
|
Combinatorial effect with methyl methanesulphonate (1) |
A network of conserved damage survival pathways revealed by a genomic RNAi screen. |
Ravi et al. |
2009 |
19543366 |
Cell line |
Kc167 |
Viability (synthetic lethal) |
Luminescence |
Version 1 |
Genome-wide |
dsRNA |
rp |
rp |
Additional information about the primary screen |
GR00128-A-2 |
35847 |
FBgn0022027 |
CG14750 |
DRSC31962 |
sp |
Synthetic lethal with methyl methanesulphonate |
|
no |
|
Combinatorial effect with methyl methanesulphonate (2) |
A network of conserved damage survival pathways revealed by a genomic RNAi screen. |
Ravi et al. |
2009 |
19543366 |
Cell line |
Kc167 |
Viability (synthetic lethal) |
Luminescence |
Version 1 |
Selected genes |
dsRNA |
rp |
rp |
|
GR00134-A-1 |
35847 |
CG14750 |
Vps25 |
38821 |
np |
Lethal |
|
no |
late pupal |
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00135-A-1 |
35847 |
CG14750 |
Vps25 |
38821 |
3.76 |
Increased noxious heat avoidance |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00138-A-1 |
35847 |
CG14750 |
|
38821 |
0.75 |
none |
|
yes |
|
Heart development and function (1) |
A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. |
Neely et al. |
2010 |
20371351 |
Tissue |
TinCΔ4 12a-Gal4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Developmental lethality |
< |
|
GR00138-A-2 |
35847 |
CG14750 |
|
38821 |
2.63 |
none |
|
no |
|
Heart development and function (2) |
A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. |
Neely et al. |
2010 |
20371351 |
Tissue |
TinCΔ4 12a-Gal4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00142-A-1 |
35847 |
CG14750 |
Vps25 |
38821 |
1.1038961038961 |
none |
|
no |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00144-A-4 |
35847 |
CG14750 |
|
38821 |
sp |
Completely lethal (pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00183-A |
35847 |
FBgn0022027 |
Vps25 |
38821 |
sp |
GFP aggregates number, GFP aggregates size, lethal |
|
no |
|
Self-renewal and differentiation in neural stem cells |
Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. |
Neumueller et al. |
2011 |
21549331 |
Tissue |
insc-GAL4 |
Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability |
Fluorescence |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
> 1 |
Additional information about a secondary screen (KK library) |
GR00189-A-1 |
35847 |
FBgn0022027 |
Vps25 |
BKN23040 |
np |
Upregulation of Notch pathway |
|
yes |
|
Notch pathway regulation (1) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Cell line |
S2 |
Notch pathway reporter |
Luminescence |
BKN |
Genome-wide |
dsRNA |
Complex, sp |
Complex criteria |
|
GR00189-A-2 |
35847 |
FBgn0022027 |
Vps25 |
38821 |
sp |
Upregulation of Notch pathway; wing disc size: overgrown; wing disc NRE:EGFP: up, broader, ectopic; wing disc myrRFP: accumulation |
|
yes |
en-GAL4 |
Notch pathway regulation (2) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
en-GAL4, C96-GAL4 and vg-GAL4 |
Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability |
Fluorescence and visual inspection |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-2 |
35847 |
FBgn0022027 |
Vps25 |
38821 |
sp |
Upregulation of Notch pathway; wing disc size: overgrown; wing disc NRE:EGFP: up, broader, ectopic; wing disc myrRFP: accumulation, reduced |
|
yes |
en-GAL4 |
Notch pathway regulation (2) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
en-GAL4, C96-GAL4 and vg-GAL4 |
Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability |
Fluorescence and visual inspection |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-2 |
35847 |
FBgn0022027 |
Vps25 |
38821 |
sp |
Upregulation of Notch pathway; wing pouch: spoon-like, crumbled |
|
yes |
C96-GAL4 |
Notch pathway regulation (2) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
en-GAL4, C96-GAL4 and vg-GAL4 |
Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability |
Fluorescence and visual inspection |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-3 |
35847 |
FBgn0022027 |
Vps25 |
np |
np |
Upregulation of Notch pathway |
|
no |
|
Notch pathway regulation (3) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
GMR-GAL4 |
Notch pathway reporter |
Fluorescence |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Average fluorescent intensity |
Complex criteria |
|
GR00190-A-1 |
35847 |
FBgn0022027 |
Vps25 |
38821 |
-0.341 |
none |
|
no |
|
Adiposity regulation (1) |
Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. |
Pospisilik et al. |
2010 |
20074523 |
Organism |
Hsp70-GAL4;Tub-GAL80ts |
Total fly triglyceride expression |
Colorimetrics |
np |
Genome-wide |
UAS-IR construct |
Triglyceride change |
Z-score > 1.65 after 3 screening rounds |
Additional information about the primary screen |
GR00191-A-1 |
35847 |
CG14750 |
Vps25 |
np |
np |
none |
|
yes |
|
Blood cell homeostasis regulation (1) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
srp-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
np |
np |
|
GR00191-A-2 |
35847 |
CG14750 |
Vps25 |
np |
np |
Increased melanotic mass formation |
|
yes |
|
Blood cell homeostasis regulation (2) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
hml∆-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
np |
np |
|
GR00191-A-3 |
35847 |
CG14750 |
Vps25 |
np |
1.8 |
none |
|
yes |
|
Blood cell homeostasis regulation (3) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
srp-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00191-A-4 |
35847 |
CG14750 |
Vps25 |
np |
0 |
none |
|
yes |
|
Blood cell homeostasis regulation (4) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
hml∆-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00191-A-5 |
35847 |
CG14750 |
Vps25 |
np |
0 |
none |
|
no |
|
Blood cell homeostasis regulation (5) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
cg-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00216-A |
35847 |
FBgn0022027 |
Vps25 |
BKN23040 |
-0.2 |
none |
|
no |
|
Wnt proteins secretion |
p24 proteins are required for secretion of Wnt ligands. |
Buechling et al. |
2011 |
22094269 |
Cell line |
S2R+ |
Wg pathway reporter |
Luminescence |
BKN |
Cellular transporting machinery genes |
dsRNA |
Z-score |
> 2.5 OR < -2.5 |
Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4) |
GR00218-S |
35847 |
CG14750 |
Vps25 |
AMB27279 |
0.46 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
35847 |
CG14750 |
|
14750R |
sp |
none |
|
no |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00282-A |
35847 |
CG14750 |
Vps25 |
38821 |
np |
none |
|
|
distorted ovaries |
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00282-A |
35847 |
CG14750 |
Vps25 |
108105 |
np |
none |
|
|
|
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
35847 |
CG14750 |
Vps25 |
108105 |
0.9288235539500489 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00311-A |
35847 |
FBgn0022027 |
Vps25 |
DRSC06486 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00315-A-1 |
35847 |
CG14750 |
Vps25 |
108105 |
np |
Effect on follicular epithelium morphology |
|
|
further phenotype data in classification screen |
Follicular epithelium development (1) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Random genes |
UAS-IR construct |
Visual inspection |
np |
Further classification screen available, see follicular epithelium development (2). |
GR00315-A-2 |
35847 |
CG14750 |
Vps25 |
108105 |
np |
Defect in formation phase |
|
|
|
Follicular epithelium development (2) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. |
GR00335-A |
35847 |
FBgn0022027 |
Vps25 |
JF02055 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
|
35847 |
FBgn0022027 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
35847 |
CG14750 |
|
38821 |
np |
liquid clearance defect |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00379-A-1 |
35847 |
CG14750 |
|
108105 |
-0.16587966126181963 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00388-A |
35847 |
CG14750 |
|
BL26286 |
|
Lethal |
|
|
ISC to EC differentiation |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
35847 |
CG14750 |
|
GD38821 |
|
Lethal |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |