Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 39222
Symbol: OXA1L
Description: OXA1L mitochondrial inner membrane protein

DataSource: http://genomernai.dkfz.de/v16/genedetails/39222

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	39222	FBgn0027615	CG6404	DRSC09672	3.717325303	Increased lipid droplet area		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	39222	FBgn0027615	CG6404	DRSC09672	2.176081296	Increased lipid droplet area		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	39222			HFA09672	0.6	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	39222			HFA09672	1.1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	39222	FBgn0027615	CG6404	GH02220	sp	none		no	library: DGC1
GR00134-A-1	39222	CG6404	CG6404	10109	np	Lethal		no	late larval	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00159-A	39222		CG6404	DRSC09672	np	none		no		Muscle assembly and maintenance	RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance.	Bai et al.	2008	18359903	Primary cells	primary embryonic cells	Muscle cell morphology	Fluorescence	np	Selected and random genes	dsRNA	Percentage of muscles with a given phenotype	Severe: > 80 %; medium: ~ 50 %
GR00214-A-1	39222	FBgn0027615	CG6404	DRSC09672	0.24	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	39222	FBgn0027615	CG6404	DRSC09672	-0.16	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	39222	FBgn0027615	CG6404	DRSC09672	1.71	Increased ERK phosphorylation after sSpitz and EGF stimulation	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	39222	FBgn0027615	CG6404	DRSC09672	0.6	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	39222	CG6404		AMB18153	0.14	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	39222	CG6404	CG6404	108091	np	none			rudimentary ovaries	piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	39222	CG6404	CG6404	108091	-0.8789932140334841	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	39222	FBgn0027615	CG6404	DRSC09672	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	39222	CG6404	CG6404	108091	np	Effect on follicular epithelium morphology			further phenotype data in classification screen	Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00315-A-2	39222	CG6404	CG6404	108091	np	Defect in formation phase				Follicular epithelium development (2)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Selected genes	UAS-IR construct	Visual inspection	np	For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00326-A	39222	FBgn0027615	CG6404	6404	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
	39222	FBgn0027615	np	np	sp	none
GR00379-A-1	39222	CG6404		108091	0.04119330216873397	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	39222	CG6404		KK108091		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.