RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 39744
  • Symbol: brm
  • Description: brahma
DataSource: http://genomernai.dkfz.de/v16/genedetails/39744

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 39744 FBgn0000212 brm DRSC11330 6.853479214 Increased lipid droplet area yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 39744 FBgn0000212 brm DRSC11330 8.695161858 Increased lipid droplet area yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00003-A-0 39744 FBgn0000212 DRSC11330 3 (0.0027) Inhibition of dengue virus propagation no Insect dengue virus infection Discovery of insect and human dengue virus host factors. Sessions et al. 2009 19396146 Cell line D.Mel-2 (S2) Dengue virus propagation High content (microscopy) DRSC dsRNA Fold inhibition (p-value) 1.5
GR00004-A-0 39744 brm DRSC11330 0.55 siRNA reporter downregulated no Drosophila small RNA pathways Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Zhou et al. 2008 19026789 Cell line S2 miRNA and siRNA pathway activity Dual luciferase DRSC dsRNA Fold change endo-siRNA reporter 1.5/0.6
GR00004-A-0 39744 brm DRSC11330 0.69 miRNA reporter downregulated no Drosophila small RNA pathways Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Zhou et al. 2008 19026789 Cell line S2 miRNA and siRNA pathway activity Dual luciferase DRSC dsRNA Fold change endo-siRNA reporter 1.5/0.6
GR00004-A-0 39744 brm DRSC11330 0.43 Endo-siRNA reporter downregulated no Drosophila small RNA pathways Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Zhou et al. 2008 19026789 Cell line S2 miRNA and siRNA pathway activity Dual luciferase DRSC dsRNA Fold change endo-siRNA reporter 1.5/0.6
GR00021-A-0 39744 FBgn0000212 DRSC11330 strong ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 39744 HFA11330 2.2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 39744 HFA11330 0.7 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00037-A-1 39744 brm np > 1.5 Upregulation of JAK/STAT pathway no JAK/STAT pathway regulation (1) Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. Baeg et al. 2005 16055650 Cell line S2-NP JAK/STAT pathway reporter Luminescence rp Genome-wide dsRNA Fold change of relative luciferase units Complex criteria
39744 FBgn0000212 brm np sp none no library: DGC1
39744 FBgn0000212 brm LD36356 sp none no library: DGC1
GR00051-A-1 39744 CG5942 brm np np Decreased Candida albicans phagocytosis yes validated (26 % cells phagocytosing) Candida albicans phagocytosis (1) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 C. albicans phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing C. albicans <
GR00051-A-2 39744 CG5942 brm np 42 Decreased Escherichia coli phagocytosis yes Candida albicans phagocytosis (2) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 Escherichia coli phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing E. coli <
GR00051-A-3 39744 CG5942 brm np 33 Decreased latex beads phagocytosis yes Candida albicans phagocytosis (3) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 Latex beads phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing latex beads <
GR00052-A 39744 brm DRSC11330 np Decreased Ca2+ uptake after thapsigargin stimulation no Store-operated calcium influx regulation Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity. Zhang et al. 2006 16751269 Cell line S2 Ca2+ uptake Fluorescence np Genome-wide dsRNA Z-score < -3
GR00062-A 39744 FBgn0000212 brm np 4 Decreased Ca2+ influx final hit Ca2+ channel regulation CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Vig et al. 2006 16645049 Cell line S2R+ Ca2+ influx Fluorescence np Genome-wide dsRNA Complex, sp >
GR00065-A 39744 CG5942 brm brm np Misrouting yes Dendrite pattern formation Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. Parrish et al. 2006 16547170 Tissue GAL4221 mCD8 protein expression Fluorescence Custom-made Transcription factors UAS-IR construct np Phenotypes in multiple blind tests Additional information about secondary screens
39744 FBgn0000212 brm np 0.638 Decreased mitochondrial citrate synthase activity yes
GR00130-A 39744 FBgn0000212 brm np >= 2 Increased mutant human huntingtin aggregation no Mutant human huntingtin aggregation RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. Doumanis et al. 2009 19789644 Cell line BG2-c2 Nhtt(62Q)EGFP aggregate number and size Fluorescence OpenBiosystems RNAi library Selected genes dsRNA Z-score Suppressor: <
GR00131-A-1 39744 brm DRSC11330 50 - 75 Altered mitochondrial Ca2+ and/or H+ levels yes Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00131-A-1 39744 brm DRSC26226 50 - 75 Altered mitochondrial Ca2+ and/or H+ levels yes Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00131-A-2 39744 brm DRSC11330, DRSC30901 50 - 75 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (2) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Selected genes dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00134-A-1 39744 CG5942 brm 37720 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 39744 CG5942 brm 37721 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 39744 CG5942 brm 37720 0.02 Developmentally lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 39744 CG5942 brm 37721 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 39744 CG5942 37721 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 39744 CG5942 brm 37721 0.723076923076923 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 39744 CG5942 37720 sp Completely lethal (pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 39744 CG5942 37721 sp Completely lethal (pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00170-A-1 39744 brahma DRSC11330 -8.5 Decreased HIF dependent transcription with desferrioxamine yes HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00170-A-2 39744 brahma DRSC30902 89 Decreased HIF dependent transcription with desferrioxamine yes group A HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-2 39744 brahma DRSC30901 85 Decreased HIF dependent transcription with desferrioxamine yes group A HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-3 39744 Brahma DRSC30902 83.9 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00170-A-3 39744 Brahma DRSC30901 84.8 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00176-A 39744 brm CG5942 HFA11330 4.87 Upregulation of Toll pathway after hEGF stimulation no Toll-dependent innate immune pathway regulation A large-scale RNAi screen identifies Deaf1 as a regulator of innate immune responses in Drosophila. Kuttenkeuler et al. 2010 20375635 Cell line SL2 Toll pathway reporter Luminescence Custom-made Transcription factors dsRNA Z-score < -2.5 OR > 2.5
GR00183-A 39744 FBgn0000212 brm 37721 sp Neuroblast more large cells, ganglion mother cell shorter lineages, neuroblast overproliferation, neuroblast underproliferation, lethal yes Self-renewal and differentiation in neural stem cells Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Neumueller et al. 2011 21549331 Tissue insc-GAL4 Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability Fluorescence np Genome-wide UAS-IR construct Phenotype strength > 1 Additional information about a secondary screen (KK library)
GR00183-A 39744 FBgn0000212 brm 37720 sp GFP aggregates size, neuroblast more large cells, ganglion mother cell shorter lineages, neuroblast overproliferation, neuroblast underproliferation, lethal yes Self-renewal and differentiation in neural stem cells Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Neumueller et al. 2011 21549331 Tissue insc-GAL4 Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability Fluorescence np Genome-wide UAS-IR construct Phenotype strength > 1 Additional information about a secondary screen (KK library)
GR00185-A 39744 CG5942 Brm np 91 none no 20 min heat shock Pol II elongation Spt6 enhances the elongation rate of RNA polymerase II in vivo. Ardehali et al. 2009 19279664 Cell line S2 Hsp70 mRNA expression Real-time qPCR Custom-made Transcription factors dsRNA Percentage <
GR00190-A-1 39744 FBgn0000212 brm 37721 -0.022 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00214-A-1 39744 FBgn0000212 brm DRSC11330 0.36 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 39744 FBgn0000212 brm DRSC11330 -2.09 Decreased ERK phosphorylation after EGF stimulation 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 39744 FBgn0000212 brm DRSC11330 2.42 Increased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 39744 FBgn0000212 brm DRSC11330 0.61 none 10 minutes sSpitz stimulation yes RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 39744 CG5942 brm AMB31683 0.02 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-4 39744 CG5942 37721 sp none no Glycosylation regulation (4) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00245-A 39744 FBgn0000212 brm DRSC11330 np Decreased formation of mutant Huntingtin (Httex1-Qn) protein aggregates yes validated Huntingtin (Htt) aggregates formation A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila. Zhang et al. 2010 20100940 Cell line S2 Mutant Httex1-Qn-eGFP protein expression and DNA content Fluorescence Version 1 and np Genome-wide dsRNA Aggregate number, size, and signal intensity > 2 standard deviations Additional information about secondary screens (reproducibility and non-overlaping dsRNAs)
GR00273-A-1 39744 FBgn0000212 brm DRSC26226 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00273-A-1 39744 FBgn0000212 brm DRSC11330 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00273-A-1 39744 FBgn0000212 brm DRSC11330 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00273-A-2 39744 FBgn0000212 brm DRSC11330 np Increased Polycomb (Pc) foci intensity Polycomb group (PcG) proteins regulation (2) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Polycomb-GFP protein localization Fluorescence DRSC Selected genes dsRNA Visual inspection np
GR00282-A 39744 CG5942 brm 37720 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 39744 CG5942 brm 37721 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 39744 CG5942 brm 37721 0.27307042398507725 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 39744 FBgn0000212 brm DRSC11330 np Actin defect, microtubule defect, other cellular phenotype cell shape processes or spiky or stretchy, increased number of actin stress fibres, increased peripheral actin, microtubule processes Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A 39744 FBgn0000212 brm HMS00050 np F1 lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 39744 FBgn0000212 brm HM04019 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 39744 FBgn0000212 brm GL00090 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
39744 FBgn0000212 np np sp none
GR00367-S 39744 CG5942 37720 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 39744 CG5942 37721 -0.31432311615991704 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 39744 CG5942 GD37721 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 39744 CG5942 BL31712 none excess of ISC or EB Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 39744 CG5942 BL34520 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.