| GR00002-A |
39881 |
FBgn0036666 |
TSG101 |
DRSC11098 |
0.360027745 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
39881 |
FBgn0036666 |
TSG101 |
DRSC11098 |
0.79057107 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00031-A-1 |
39881 |
|
|
HFA11098 |
1.1 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
39881 |
|
|
HFA11098 |
0.4 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00038-A-0 |
39881 |
FBgn0036666 |
TSG101 |
np |
* |
Escape pff LLO- strain |
|
no |
|
L. monocytogenes infection |
Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. |
Cheng et al. |
2005 |
16157870 |
Cell line |
S2 |
L. monocytogenes infection |
High content (microscopy) |
Custom-made |
|
dsRNA |
Visual inspection |
np |
|
GR00038-A-0 |
39881 |
FBgn0036666 |
TSG101 |
np |
* |
Fewer infected cells |
|
no |
|
L. monocytogenes infection |
Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. |
Cheng et al. |
2005 |
16157870 |
Cell line |
S2 |
L. monocytogenes infection |
High content (microscopy) |
Custom-made |
|
dsRNA |
Visual inspection |
np |
|
GR00044-A |
39881 |
|
TSG101 |
HFA11098 |
3.4 |
Upregulation of JAK/STAT pathway |
|
no |
|
JAK/STAT pathway regulation |
Identification of JAK/STAT signalling components by genome-wide RNA interference. |
Mueller et al. |
2005 |
16094372 |
Cell line |
Kc167 |
JAK/STAT pathway reporter |
Luminescence |
HFA |
Genome-wide |
dsRNA |
Z-score |
> 2 OR < -2 |
|
|
39881 |
FBgn0036666 |
TSG101 |
RE26756 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
39881 |
FBgn0036666 |
TSG101 |
RH36765 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00069-A-0 |
39881 |
FBgn0036666 |
dTsg101 |
np |
np |
Increased M. smegmatis infection |
|
no |
|
M. smegmatis infection |
ESCRT factors restrict mycobacterial growth. |
Philips et al. |
2008 |
18287038 |
Cell line |
S2 |
M. smegmatis infection |
High content (microscopy) |
DRSC |
|
dsRNA |
np |
np |
|
GR00076-A |
39881 |
CG9712 |
TSG101 |
np |
3.2 |
none |
|
no |
|
CD4 regulation by human immunodeficiency virus Type 1 Nef (HIV-1 Nef) |
Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor. |
Chaudhuri et al. |
2007 |
17267500 |
Cell line |
S2 |
Human CD4 surface protein expression |
Fluorescence |
Expression Arrest Drosophila RNAi library |
Protein trafficking machinery genes |
dsRNA |
Fold downregulation |
np |
|
GR00134-A-1 |
39881 |
CG9712 |
TSG101 |
23944 |
np |
Lethal |
|
no |
late pupal |
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00138-A-1 |
39881 |
CG9712 |
|
23944 |
1 |
none |
|
yes |
|
Heart development and function (1) |
A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. |
Neely et al. |
2010 |
20371351 |
Tissue |
TinCΔ4 12a-Gal4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Developmental lethality |
< |
|
GR00138-A-2 |
39881 |
CG9712 |
|
23944 |
2.53 |
none |
|
no |
|
Heart development and function (2) |
A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. |
Neely et al. |
2010 |
20371351 |
Tissue |
TinCΔ4 12a-Gal4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00142-A-1 |
39881 |
CG9712 |
TSG101 |
23944 |
-0.464646464646464 |
none |
|
no |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00144-A-4 |
39881 |
CG9712 |
|
23944 |
sp |
Completely lethal (pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-1 |
39881 |
FBgn0036666 |
TSG101 |
BKN28961 |
np |
Upregulation of Notch pathway |
|
yes |
|
Notch pathway regulation (1) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Cell line |
S2 |
Notch pathway reporter |
Luminescence |
BKN |
Genome-wide |
dsRNA |
Complex, sp |
Complex criteria |
|
GR00189-A-2 |
39881 |
FBgn0036666 |
TSG101 |
23944 |
sp |
Downregulation of Notch pathway; adult wing margin: notches |
|
yes |
en-GAL4; final gene scoring: up- and downregulation of Notch pathway |
Notch pathway regulation (2) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
en-GAL4, C96-GAL4 and vg-GAL4 |
Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability |
Fluorescence and visual inspection |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-2 |
39881 |
FBgn0036666 |
TSG101 |
23944 |
sp |
Upregulation of Notch pathway; delay of larval development; wing disc size: overgrown; wing disc NRE:EGFP: up, broader, ectopic; wing disc myrRFP: accumulation; lethal |
|
yes |
en-GAL4; final gene scoring: up- and downregulation of Notch pathway |
Notch pathway regulation (2) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
en-GAL4, C96-GAL4 and vg-GAL4 |
Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability |
Fluorescence and visual inspection |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-3 |
39881 |
FBgn0036666 |
TSG101 |
np |
np |
Upregulation of Notch pathway |
|
no |
|
Notch pathway regulation (3) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
GMR-GAL4 |
Notch pathway reporter |
Fluorescence |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Average fluorescent intensity |
Complex criteria |
|
GR00218-S |
39881 |
CG9712 |
|
AMB23094 |
0.22 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
39881 |
CG9712 |
|
9712R |
sp |
none |
|
no |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00245-A |
39881 |
FBgn0036666 |
TSG101 |
DRSC11098 |
np |
Decreased formation of mutant Huntingtin (Httex1-Qn) protein aggregates |
|
yes |
validated |
Huntingtin (Htt) aggregates formation |
A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila. |
Zhang et al. |
2010 |
20100940 |
Cell line |
S2 |
Mutant Httex1-Qn-eGFP protein expression and DNA content |
Fluorescence |
Version 1 and np |
Genome-wide |
dsRNA |
Aggregate number, size, and signal intensity |
> 2 standard deviations |
Additional information about secondary screens (reproducibility and non-overlaping dsRNAs) |
GR00282-A |
39881 |
CG9712 |
TSG101 |
23944 |
np |
Increased transposon expression |
|
|
|
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
39881 |
CG9712 |
TSG101 |
23944 |
0.8340054797419065 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00308-A |
39881 |
CG9712 |
|
np |
3 |
Decreased cell aggregation |
|
|
|
Cadherin-mediated cell-cell adhesion |
A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion |
Toret et al. |
2014 |
24446484 |
Cell line |
S2 |
Cell aggregation |
Fluorescence |
V2 RNAi library (Thermo Fisher Scientific) |
Genome-wide |
dsRNA |
Visual inspection; average of 3 replicates; 0 |
> 1.5 |
Additional secondary screen available. S2 cells stably express DE-cadherin. |
GR00311-A |
39881 |
FBgn0036666 |
TSG101 |
DRSC11098 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00335-A |
39881 |
FBgn0036666 |
TSG101 |
HMS01768 |
np |
Embryonic lethal |
|
|
some embryonic lethal |
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
|
39881 |
FBgn0036666 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
39881 |
CG9712 |
|
23944 |
np |
lethal or adult morphology phenotype |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00379-A-1 |
39881 |
CG9712 |
|
23944 |
-0.34620898572420655 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00388-A |
39881 |
CG9712 |
|
GD23944 |
|
Lethal |
|
|
ISC to EC differentiation |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |