RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 40201
  • Symbol: gig
  • Description: gigas
DataSource: http://genomernai.dkfz.de/v16/genedetails/40201

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00001-A 40201 FBgn0005198 gig DRSC11351 0.76 none yes Cell size regulation PDGF/VEGF signaling controls cell size in Drosophila. Sims et al. 2009 19216764 Cell line S2R+ F-actin and microtubule protein expression and DNA content Fluorescence rp Genome-wide dsRNA Cell area Z-score < -2
GR00002-A 40201 FBgn0005198 gig DRSC11351 1.220981313 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 40201 FBgn0005198 gig DRSC11351 1.951113871 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 40201 FBgn0005198 gig DRSC11351 0.057444689 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00013-A 40201 CG6975 gig np np Decreased Chlamydia caviae infection yes Chlamydia caviae infection RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection. Derre et al. 2007 17967059 Cell line SL2 Chlamydia MOMP and LPS expression and DNA content Fluorescence np Genome-wide dsRNA Visual inspection np Additional information about secondary screens
GR00014-A 40201 gig DRSC11351 strong Decreased Flag-Mad nuclear accumulation no Dpp pathway regulation Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. Xu et al. 2007 17785517 Cell line S2R+ Flag-Mad protein expression and subcellular location Fluorescence rp Genome-wide dsRNA Visual inspection np
GR00021-A-0 40201 FBgn0005198 DRSC11351 strong ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00026-A-0 40201 FBgn0005198 gigas np np Morphology no Cell morphology A functional genomic analysis of cell morphology using RNA interference. Kiger et al. 2003 14527345 Cell line Kc167, S2R+ Morphology High content (microscopy) Custom-made dsRNA Visual inspection np
GR00031-A-1 40201 HFA11351 -0.2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 40201 HFA11351 0.2 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
40201 FBgn0005198 gig LD36178 sp none no library: DGC1
GR00051-A-1 40201 CG6975 gig np np Decreased Candida albicans phagocytosis yes validated (12 % cells phagocytosing) Candida albicans phagocytosis (1) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 C. albicans phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing C. albicans <
GR00051-A-2 40201 CG6975 gig np 34 Decreased Escherichia coli phagocytosis yes Candida albicans phagocytosis (2) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 Escherichia coli phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing E. coli <
GR00051-A-3 40201 CG6975 gig np 28 Decreased latex beads phagocytosis yes Candida albicans phagocytosis (3) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 Latex beads phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing latex beads <
GR00088-A 40201 CG6975 gig np np GFP protein expression under hypoxia Translational activity under hypoxia An RNA interference screen identifies a novel regulator of target of rapamycin that mediates hypoxia suppression of translation in Drosophila S2 cells. Lee et al. 2008 18653470 Cell line S2 GFP protein expression Western blot Custom-made Selected genes dsRNA np np
GR00128-A-1 40201 FBgn0005198 CG6975 DRSC11351 sp none no hit according to not so stringent primary analysis Combinatorial effect with methyl methanesulphonate (1) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Genome-wide dsRNA rp rp Additional information about the primary screen
GR00128-A-2 40201 FBgn0005198 CG6975 DRSC29781 sp Increased viability with methyl methanesulphonate no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00134-A-1 40201 CG6975 gig 6313 np Weak flyer no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 40201 CG6975 gig 6313 0.46 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 40201 CG6975 gig 6314 -0.4 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 40201 CG6975 6313 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00139-A 40201 gig 6313 sp Lethal no Fly viability and adult morphology A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Dietzl et al. 2007 17625558 Organism Act5C-GAL4 Adult morphology and viability Visual inspection Custom-made Selected and random genes UAS-IR construct Phenotype strength np Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1 40201 CG6975 gig 6314 -0.802469135802469 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00142-A-1 40201 CG6975 gig 6314 -0.755555555555555 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 40201 CG6975 6313 sp Gain of bristles, loss of bristles, bristle morphology defects, notum malformation death, overproliferation, lateral inhibition yes not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 40201 CG6975 6314 sp Colour defects, bristle morphology defects, notum malformation death, overproliferation yes partially lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-5 40201 CG6975 6313 np Bent up (spoon-shaped) wing, wrinkled wings no Notch pathway regulation (5) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue MS1096-GAL4 Wing morphology and viability Visual inspection np Selected genes UAS-IR construct Visual inspection np
GR00159-A 40201 gig DRSC11351 np none no Muscle assembly and maintenance RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Bai et al. 2008 18359903 Primary cells primary embryonic cells Muscle cell morphology Fluorescence np Selected and random genes dsRNA Percentage of muscles with a given phenotype Severe: > 80 %; medium: ~ 50 %
GR00169-A 40201 FBgn0005198 gig DRSC11351 -3.5 Decreased dAkt phosphorylation after insulin stimulation 10 min insulin stimulation no Akt-TOR pathway negative feedback regulation Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. Kockel et al. 2010 20585550 Cell line Kc167 dAkt phosphorylation Fluorescence Custom-made Genome-wide dsRNA Z-score < -2.5 OR > 2.5
GR00190-A-1 40201 FBgn0005198 gig 6314 -0.399 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00214-A-1 40201 FBgn0005198 gig DRSC11351 0.21 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 40201 FBgn0005198 gig DRSC11351 0.31 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 40201 FBgn0005198 gig DRSC11351 -0.47 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 40201 FBgn0005198 gig DRSC11351 -0.8 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00216-A 40201 FBgn0005198 gig BKN20742 -1.2 none no Wnt proteins secretion p24 proteins are required for secretion of Wnt ligands. Buechling et al. 2011 22094269 Cell line S2R+ Wg pathway reporter Luminescence BKN Cellular transporting machinery genes dsRNA Z-score > 2.5 OR < -2.5 Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S 40201 CG6975 gig AMB18760 -1.32 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 40201 CG6975 6975R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00273-A-1 40201 FBgn0005198 gig DRSC29781 2 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00273-A-2 40201 FBgn0005198 gig DRSC29781 np Decreased Polycomb (Pc) foci intensity Polycomb group (PcG) proteins regulation (2) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Polycomb-GFP protein localization Fluorescence DRSC Selected genes dsRNA Visual inspection np
GR00282-A 40201 CG6975 gig 6313 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 40201 CG6975 gig 6314 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 40201 CG6975 gig 103417 -0.4739805255799101 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 40201 FBgn0005198 gig DRSC11351 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A 40201 FBgn0005198 gig GL00321 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 40201 FBgn0005198 gig HMS01217 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 40201 FBgn0005198 gig HM04083 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
40201 FBgn0005198 np np sp none
GR00367-S 40201 CG6975 6313 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 40201 CG6975 103417 0.053219864465816495 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 40201 CG6975 BL34737 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 40201 CG6975 GD6313 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 40201 CG6975 GD6314 none large nucleus Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.