Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 40444
Symbol: Act79B
Description: Actin 79B

DataSource: http://genomernai.dkfz.de/v16/genedetails/40444

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	40444	FBgn0000045	Act79B	DRSC11604	-0.82383536	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	40444	FBgn0000045	Act79B	DRSC11604	-2.438852219	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00013-A	40444	CG7478	Act79B	np	np	Decreased Chlamydia caviae infection		yes		Chlamydia caviae infection	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.	Derre et al.	2007	17967059	Cell line	SL2	Chlamydia MOMP and LPS expression and DNA content	Fluorescence	np	Genome-wide	dsRNA	Visual inspection	np	Additional information about secondary screens
GR00021-A-0	40444	FBgn0000045		DRSC11604	weak	ERK activation reduced		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	40444			HFA11604	1.2	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	40444			HFA11604	1.2	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	40444	FBgn0000045	Act79B	DRSC11604	medium	Binucleate cells with microtubule extensions		no
GR00034-A	40444		Actin	np	12	Increased Dipt::lacZ protein expression			without lipopolysaccharide stimulation, CDRi	Imd pathway regulation	Functional dissection of an innate immune response by a genome-wide RNAi screen.	Foley and O'Farrell	2004	15221030	Cell line	S2	Dipt::lacZ protein expression	Colorimetrics	Custom-made	Selected genes	dsRNA	Percentage LacZ-positive cells	Complex criteria	Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish)
GR00034-A	40444		Actin	np	22	Increased Dipt::lacZ protein expression after lipopolysaccharide stimulation			with lipopolysaccharide stimulation, EDRi	Imd pathway regulation	Functional dissection of an innate immune response by a genome-wide RNAi screen.	Foley and O'Farrell	2004	15221030	Cell line	S2	Dipt::lacZ protein expression	Colorimetrics	Custom-made	Selected genes	dsRNA	Percentage LacZ-positive cells	Complex criteria	Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish)
GR00084-A-0	40444	FBgn0000045	CG7478	np	np	C. trachomatis infection down		no		C. trachomatis infection	RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry.	Elwell et al.	2008	18369471	Cell line	S2	C. trachomatis infection	High content (microscopy)	OpenBiosystems		dsRNA	Visual inspection	np
GR00130-A	40444	FBgn0000045	Act79B	np	1.5 - 2	Increased mutant human huntingtin aggregation		no		Mutant human huntingtin aggregation	RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation.	Doumanis et al.	2009	19789644	Cell line	BG2-c2	Nhtt(62Q)EGFP aggregate number and size	Fluorescence	OpenBiosystems RNAi library	Selected genes	dsRNA	Z-score	Suppressor: <
GR00134-A-1	40444	CG7478	Act79B	9776	np	Lethal		yes		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-2	40444	CG7478	Act79B	np	np	Wing posture / locomotion / ability to fly defect or lethal / semi-lethal		no		Muscle morphogenesis and function (2)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	Custom-made	Selected genes	UAS-IR construct	rp	S19 > 0.5
GR00142-A-1	40444	CG7478	Act79B	9776	1.78666666666666	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	40444	CG7478		9776	sp	Completely lethal (pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00214-A-1	40444	FBgn0000045	Act79B	DRSC11604	-1.52	Decreased ERK phosphorylation after EGF stimulation	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	40444	FBgn0000045	Act79B	DRSC11604	0.43	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	40444	FBgn0000045	Act79B	DRSC11604	1.73	Increased ERK phosphorylation after sSpitz and EGF stimulation	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	40444	FBgn0000045	Act79B	DRSC11604	0.94	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00216-A	40444	FBgn0000045	Act79B	BKN32081	-0.6	none		no		Wnt proteins secretion	p24 proteins are required for secretion of Wnt ligands.	Buechling et al.	2011	22094269	Cell line	S2R+	Wg pathway reporter	Luminescence	BKN	Cellular transporting machinery genes	dsRNA	Z-score	> 2.5 OR < -2.5	Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S	40444	CG7478	Act79B	AMB30628	0.16	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00238-S-1	40444	CG7478	Act79B	7478R-2	sp	Climbing defect		yes	library: NIG	Muscle development and maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	Mef2-GAL4	Climbing ability and viability	Negative geotaxis assay and visual inspection	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-1	40444	CG7478	Act79B	102588	sp	Embryo lethal		yes	library: VDRC	Muscle development and maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	Mef2-GAL4	Climbing ability and viability	Negative geotaxis assay and visual inspection	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2	40444	CG7478	Act79B	7478R-2	sp	Climbing defect		no	library: NIG	Muscle maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	TARGET Mef2-GAL4	Climbing ability	Negative geotaxis assay	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2	40444	CG7478	Act79B	102588	sp	Climbing defect		no	library: VDRC	Muscle maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	TARGET Mef2-GAL4	Climbing ability	Negative geotaxis assay	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1	40444	CG7478		7478R	np	Decreased Chp protein expression		no	not detectable Chp level	Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00273-A-1	40444	FBgn0000045	Act79B	DRSC11604	3	Effect on Polycomb (Pc) foci			Weakly expressed	Polycomb group (PcG) proteins regulation (1)	Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen	Gonzalez et al.	2014	24703951	Cell line	S2	Pc-GFP protein localization	Fluorescence	DRSC	Genome-wide and transcription factors	dsRNA	Visual inspection	1	Additional phenotypic clustering screen available.
GR00273-A-2	40444	FBgn0000045	Act79B	DRSC11604	np	Decreased Polycomb (Pc) foci intensity			Weakly expressed	Polycomb group (PcG) proteins regulation (2)	Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen	Gonzalez et al.	2014	24703951	Cell line	S2	Polycomb-GFP protein localization	Fluorescence	DRSC	Selected genes	dsRNA	Visual inspection	np
GR00284-A	40444	CG7478	Act79B	102588	0.7222796077759358	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	40444	FBgn0000045	Act79B	DRSC11604	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	40444	FBgn0000045	Act79B	HMS00303	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	40444	FBgn0000045	np	np	sp	none
GR00367-S	40444	CG7478		102588	np	liquid clearance defect			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	40444	CG7478		102588	0.00994049614608318	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	40444	CG7478		KK102588		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	40444	CG7478		GD9776		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.