RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 40863
• Symbol: lap
• Description: like-AP180

DataSource: http://genomernai.dkfz.de/v16/genedetails/40863

Export (tab separated) Export to Excel

Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	40863	FBgn0026210	lap	DRSC12628	-0.007102911	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	40863	FBgn0026210	lap	DRSC12628	0.006475184	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	40863			HFA12628	0	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	40863			HFA12628	-0.9	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00047-A-1	40863	FBgn0026210	lap	DRSC12628	sp	Decreased horseradish peroxidase protein expression		no		Constitutive protein secretion and Golgi organization (1)	Functional genomics reveals genes involved in protein secretion and Golgi organization.	Bard et al.	2006	16452979	Cell line	S2	Horseradish peroxidase protein expression	Luminescence		Genome-wide	dsRNA	Z-score	< -1.5
	40863	FBgn0015772, FBgn0026210	Nak, lap	SD07306	sp	none		no	library: DGC1
	40863	FBgn0026210	lap	RH47395	sp	none		no	library: DGC2
	40863	FBgn0026210, FBgn0052111	lap, CG32111	GM10787	sp	none		no	library: DGC1
GR00076-A	40863	CG2520	CALM/AP180	np	2.82	none		no		CD4 regulation by human immunodeficiency virus Type 1 Nef (HIV-1 Nef)	Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor.	Chaudhuri et al.	2007	17267500	Cell line	S2	Human CD4 surface protein expression	Fluorescence	Expression Arrest Drosophila RNAi library	Protein trafficking machinery genes	dsRNA	Fold downregulation	np
GR00134-A-1	40863	CG2520	lap	12731	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	40863	CG2520	lap	12732	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	40863	CG2520	lap	12732	-1.17	Developmentally lethal		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	40863	CG2520		12732	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00142-A-1	40863	CG2520	CG2520	12731	-2.49523809523809	Decreased viability after Serratia marcescens infection		yes		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00142-A-2	40863	CG2520	CG2520	np	sp	Decreased viability after Serratia marcescens infection		yes		Serratia marcescens infection (2)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Tissue	HML-GAL4	Viability	Fly count	np	Selected genes	UAS-IR construct	Complex, sp	Significantly reduced survival (p < 0.05)
GR00142-A-3	40863	CG2520	CG2520	np	np	Viable		yes		Serratia marcescens infection (3)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Tissue	HML-GAL4	Viability under non-pathogenic conditions	np	np	Selected genes	UAS-IR construct	np	np
GR00142-A-4	40863	CG2520	CG2520	np	sp	Decreased viability after Serratia marcescens infection		no		Serratia marcescens infection (4)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Tissue	NP1-GAL4	Viability	Fly count	np	Selected genes	UAS-IR construct	Complex, sp	Significantly reduced survival (p < 0.05)
GR00144-A-4	40863	CG2520		12731	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	40863	CG2520		12732	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00216-A	40863	FBgn0086372	lap	BKN29279	0.6	none		no		Wnt proteins secretion	p24 proteins are required for secretion of Wnt ligands.	Buechling et al.	2011	22094269	Cell line	S2R+	Wg pathway reporter	Luminescence	BKN	Cellular transporting machinery genes	dsRNA	Z-score	> 2.5 OR < -2.5	Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S	40863	CG2520	lap	AMB34810	0.12	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	40863	CG2520	lap	12731	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	40863	CG2520	lap	12732	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	40863	CG2520	lap	105767	0.13026122808342455	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00308-A	40863	CG2520	lap	np	2	Decreased cell aggregation				Cadherin-mediated cell-cell adhesion	A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion	Toret et al.	2014	24446484	Cell line	S2	Cell aggregation	Fluorescence	V2 RNAi library (Thermo Fisher Scientific)	Genome-wide	dsRNA	Visual inspection; average of 3 replicates; 0	> 1.5	Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A	40863	FBgn0086372	lap	DRSC12628	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	40863	CG2520	lap	105767	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00326-A	40863	FBgn0086372	lap	105767	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00335-A	40863	FBgn0086372	lap	JF02994	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	40863	FBgn0086372	np	np	sp	none
	40863	FBgn0086372	np	np	-2.93	Increased transposon expression			fold change: 18.57
GR00339-A-2	40863	FBgn0086372	lap	12731	3	Increased transposon expression				piRNA pathway regulation (2)	A Genome-wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary piRNA Biogenesis in Drosophila	Muerdter et al.	2013	23665228	Tissue	traffic jam GAL4	Gypsy and ZAM transposon expression	qPCR	VDRC (GD, KK) and TRiP	Selected genes	UAS-IR construct	Fold change	>	Following transposons were used for scoring: Gypsy for KK and ZAM for GD RNAi lines. TRIP reagents als TRIP_11111 dargestellt, Suchbarkeit? Developmental defect: wurden nicht gemessen, passt np als Score?
GR00367-S	40863	CG2520		12731	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	40863	CG2520		105767	-0.38186378541360855	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	40863	CG2520		BL28358		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	40863	CG2520		KK105767		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.