RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 41104
  • Symbol: ATP6AP2
  • Description: ATPase H+ transporting accessory protein 2
DataSource: http://genomernai.dkfz.de/v16/genedetails/41104

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 41104 FBgn0037671 CG8444 DRSC16420 -1.095161693 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 41104 FBgn0037671 CG8444 DRSC16420 -0.212320834 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1 41104 HFA16420 -1.6 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 41104 HFA16420 -1.9 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
41104 FBgn0037671 CG8444 LD30634 sp none no library: DGC1
GR00134-A-1 41104 CG8444 CG8444 5830 np Lethal yes adult/pharate Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-2 41104 CG8444 CG8444 np np Wing posture / locomotion / ability to fly defect or lethal / semi-lethal no Muscle morphogenesis and function (2) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection Custom-made Selected genes UAS-IR construct rp S19 > 0.5
GR00135-A-1 41104 CG8444 CG8444 5830 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 41104 CG8444 5830 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 41104 CG8444 CG8444 5830 0.333333333333333 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 41104 CG8444 5830 sp Bristle morphology defects, planar polarity defects, notum malformation death no partially lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00189-A-1 41104 FBgn0037671 CG8444 BKN27676 np Upregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 41104 FBgn0037671 CG8444 8444R-1 np none yes C96-GAL4; final gene scoring: lethal and/or indeterminable effect on Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 41104 FBgn0037671 CG8444 8444R-3 np none yes C96-GAL4; final gene scoring: lethal and/or indeterminable effect on Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 41104 FBgn0037671 CG8444 8444R-1 sp Adult wing veins: disrupted, broader; wing disc NRE:EGFP: up, gap; wing disc myrRFP: accumulation; lethal yes en-GAL4; final gene scoring: lethal and/or indeterminable effect on Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 41104 FBgn0037671 CG8444 8444R-3 sp Adult wing veins: disrupted; adult wing posterior compartment: smaller, crumbled yes en-GAL4; final gene scoring: lethal and/or indeterminable effect on Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 41104 FBgn0037671 CG8444 8444R-1 sp Wing disc NRE:EGFP: up; lethal yes en-GAL4 Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 41104 FBgn0037671 CG8444 np np none no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00190-A-1 41104 FBgn0037671 CG8444 5830 1.566 Increased triglyceride expression yes Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00190-A-2 41104 FBgn0037671 CG8444 5830 d/l Lethal or developmental delay no Adiposity regulation (2) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue nsyb-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-3 41104 FBgn0037671 CG8444 5830 -0.11 none no Adiposity regulation (3) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue C57-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-4 41104 FBgn0037671 CG8444 5830 0.565 Increased triglyceride expression no Adiposity regulation (4) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue oe-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-5 41104 FBgn0037671 CG8444 5830 0.381 Increased triglyceride expression no Adiposity regulation (5) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue ppl-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00191-A-1 41104 CG8444 CG8444 np np none yes Blood cell homeostasis regulation (1) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-2 41104 CG8444 CG8444 np np Increased melanotic mass formation yes Blood cell homeostasis regulation (2) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-3 41104 CG8444 CG8444 np 3 none yes Blood cell homeostasis regulation (3) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-4 41104 CG8444 CG8444 np 0 none yes Blood cell homeostasis regulation (4) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-5 41104 CG8444 CG8444 np 10 Increased melanotic mass formation yes Blood cell homeostasis regulation (5) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-6 41104 CG8444 CG8444 np 2 none yes confirmed melanotic suppressor gene, overlapping dsRNA Blood cell homeostasis regulation (6) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-7 41104 CG8444 CG8444 np 0 none yes confirmed melanotic suppressor gene, overlapping dsRNA Blood cell homeostasis regulation (7) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-8 41104 CG8444 CG8444 np 13.5 Increased melanotic mass formation no confirmed melanotic suppressor gene, overlapping dsRNA Blood cell homeostasis regulation (8) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00218-S 41104 CG8444 AMB30726 0.73 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 41104 CG8444 8444R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 41104 CG8444 VhaM8.9 105281 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 41104 CG8444 VhaM8.9 105281 0.8000164425072774 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00296-A 41104 CG8444 np -3.79 Decreased Sindbis virus (SINV) infection prim. screen rep. B: -1.96; sec. val. screen: -2.83 Sindbis virus (SINV) infection Genome-wide RNAi Screen Identifies SEC61A and VCP as Conserved Regulators of Sindbis Virus Entry Panda et al. 2013 24332855 Cell line DL1 Sindbis virus (SINV) GFP protein expression Fluorescence Ambion Genome-wide dsRNA Z-score < -2 OR > 2 Primary screen performed in duplicate. Z-scores from replicate B and from secondary validation screen are shown in the comment field. Recombinant SINV (HRsp) expressing GFP was used for infection.
GR00311-A 41104 FBgn0037671 CG8444 DRSC16420 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 41104 CG8444 VhaM8.9 105281 np Effect on follicular epithelium morphology further phenotype data in classification screen Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00315-A-2 41104 CG8444 VhaM8.9 105281 np Defect in formation and maturation phase Follicular epithelium development (2) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Selected genes UAS-IR construct Visual inspection np For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
41104 FBgn0037671 np np sp none
GR00367-S 41104 CG8444 5830 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 41104 CG8444 105281 -0.3542110396791622 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 41104 CG8444 GD5830 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 41104 CG8444 KK105281 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.