| GR00002-A |
41182 |
FBgn0037735 |
CG16899 |
DRSC15159 |
-0.033700025 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
41182 |
FBgn0037735 |
CG16899 |
DRSC15159 |
-0.461536259 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
41182 |
FBgn0052937 |
CG32937 |
DRSC21961 |
-1.119279547 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
41182 |
FBgn0052937 |
CG32937 |
DRSC21961 |
0.980049269 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00014-A |
41182 |
|
FoxP |
DRSC21961 |
strong |
Decreased Flag-Mad nuclear accumulation |
|
no |
|
Dpp pathway regulation |
Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. |
Xu et al. |
2007 |
17785517 |
Cell line |
S2R+ |
Flag-Mad protein expression and subcellular location |
Fluorescence |
rp |
Genome-wide |
dsRNA |
Visual inspection |
np |
|
GR00031-A-1 |
41182 |
|
|
HFA15159 |
-0.9 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
41182 |
|
|
HFA15159 |
0.3 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00062-A |
41182 |
FBgn0052937 |
CG32937 |
np |
1 |
Decreased Ca2+ influx |
|
|
|
Ca2+ channel regulation |
CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. |
Vig et al. |
2006 |
16645049 |
Cell line |
S2R+ |
Ca2+ influx |
Fluorescence |
np |
Genome-wide |
dsRNA |
Complex, sp |
> |
|
GR00065-A |
41182 |
CG16899 |
CG16899 |
CG16899 |
np |
none |
|
no |
|
Dendrite pattern formation |
Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. |
Parrish et al. |
2006 |
16547170 |
Tissue |
GAL4221 |
mCD8 protein expression |
Fluorescence |
Custom-made |
Transcription factors |
UAS-IR construct |
np |
Phenotypes in multiple blind tests |
Additional information about secondary screens |
GR00134-A-1 |
41182 |
CG16899 |
CG16899 |
15732 |
np |
none |
|
no |
|
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00134-A-1 |
41182 |
CG16899 |
CG16899 |
15735 |
np |
none |
|
no |
|
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00135-A-1 |
41182 |
CG32937 |
CG32937 |
50200 |
0.12 |
none |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-1 |
41182 |
CG16899 |
CG16899 |
15735 |
-0.69 |
none |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-1 |
41182 |
CG16899 |
CG16899 |
15732 |
2.12 |
Increased noxious heat avoidance |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-2 |
41182 |
CG16899 |
CG16899 |
15732 |
33.66666667 |
none |
|
no |
|
Heat nociception (2) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Temperature-induced paralysis |
Exposure to 46 oC |
np |
Selected genes |
UAS-IR construct |
Mean kinetics |
Significant increase |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00138-A-1 |
41182 |
CG16899 |
|
15732 |
1 |
none |
|
no |
|
Heart development and function (1) |
A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. |
Neely et al. |
2010 |
20371351 |
Tissue |
TinCΔ4 12a-Gal4 |
Viability |
Fly count |
np |
Selected genes |
UAS-IR construct |
Developmental lethality |
< |
|
GR00142-A-1 |
41182 |
CG16899 |
CG16899 |
15732 |
1.16666666666666 |
none |
|
no |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00144-A-4 |
41182 |
CG16899 |
|
15732 |
0 |
none |
|
no |
not lethal |
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-4 |
41182 |
CG16899 |
|
15735 |
0 |
none |
|
no |
not lethal |
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00148-A |
41182 |
FBgn0052937 |
CG32937 |
np |
1.99 |
Increased P-JNK protein expression with PGN |
15 min PGN induction |
no |
|
PGN-induced dJNK phosphorylation |
A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. |
Bond and Foley |
2009 |
19893628 |
Cell line |
S2 |
P-JNK protein expression |
Fluorescence |
Custom-made |
Genome-wide |
dsRNA |
Z-score |
Complex criteria |
Only hits stored in GenomeRNAi |
GR00190-A-1 |
41182 |
FBgn0037735 |
CG16899 |
15732 |
-0.502 |
none |
|
no |
|
Adiposity regulation (1) |
Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. |
Pospisilik et al. |
2010 |
20074523 |
Organism |
Hsp70-GAL4;Tub-GAL80ts |
Total fly triglyceride expression |
Colorimetrics |
np |
Genome-wide |
UAS-IR construct |
Triglyceride change |
Z-score > 1.65 after 3 screening rounds |
Additional information about the primary screen |
GR00214-A-1 |
41182 |
FBgn0262477 |
fd85E |
DRSC29407 |
-0.09 |
none |
10 minutes EGF stimulation |
yes |
|
RTK-Ras-ERK pathway regulation (1) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
S2R+ |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00214-A-1 |
41182 |
FBgn0262477 |
fd85E |
DRSC29407 |
0.73 |
none |
0 minutes EGF stimulation |
yes |
|
RTK-Ras-ERK pathway regulation (1) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
S2R+ |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00214-A-2 |
41182 |
FBgn0262477 |
fd85E |
DRSC29407 |
2.47 |
Increased ERK phosphorylation after sSpitz and EGF stimulation |
10 minutes sSpitz stimulation |
no |
|
RTK-Ras-ERK pathway regulation (2) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
Kc167 |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00214-A-2 |
41182 |
FBgn0262477 |
fd85E |
DRSC29407 |
0.23 |
none |
30 minutes sSpitz stimulation |
no |
|
RTK-Ras-ERK pathway regulation (2) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
Kc167 |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00218-S |
41182 |
CG16899 |
FoxP |
AMB20899 |
0.64 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00218-S |
41182 |
CG32937 |
FoxP |
AMB31977 |
-0.95 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
41182 |
CG16899 |
|
16899R |
sp |
none |
|
no |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00311-A |
41182 |
FBgn0037735 |
CG16899 |
DRSC15159 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00326-A |
41182 |
FBgn0030364 |
CG15735 |
15735 |
np |
none |
|
|
|
Synapse formation and maintenance |
A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance |
Valakh et al. |
2012 |
22542760 |
Tissue |
Elav-Gal4 |
Neuromuscular junction morphology |
Fluorescence |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
For further phenotype explanations sp. |
|
41182 |
FBgn0262477 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
41182 |
np |
np |
HFA15159 |
-0.36 |
none |
|
|
fold change: 1.2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
41182 |
CG43067 |
|
15735 |
np |
none |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00388-A |
41182 |
CG32937 |
|
GD50200 |
|
none |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
41182 |
CG16899 |
|
GD15732 |
|
none |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
41182 |
CG43067 |
|
BL26774 |
|
none |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |