RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 41839
  • Symbol: tefu
  • Description: telomere fusion
DataSource: http://genomernai.dkfz.de/v16/genedetails/41839

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 41839 FBgn0045035 tefu DRSC16060 -0.216568327 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 41839 FBgn0045035 tefu DRSC16060 1.883014019 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00030-A-1 41839 CG6535 CG6535 CG6535 np none yes Cell cycle regulation (1) Genome-wide survey of protein kinases required for cell cycle progression. Bettencourt-Dias et al. 2004 15616552 Cell line S2 alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content Flow cytometry rp Kinases dsRNA Confidence intervals Complex criteria
GR00030-A-2 41839 CG6535 CG6535 CG6535 np none no Cell cycle regulation (2) Genome-wide survey of protein kinases required for cell cycle progression. Bettencourt-Dias et al. 2004 15616552 Cell line S2 Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression Flow cytometry rp Kinases and kinase regulators dsRNA np np
GR00031-A-1 41839 HFA16060 -0.5 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 41839 HFA16060 0.8 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
41839 FBgn0045035 tefu CT20331 sp none no library: PHOSPHO
41839 FBgn0045035 tefu AT01448 sp none no library: DGC2
GR00070-A-0 41839 FBgn0045035 Tefu np np none no Endocytosis Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation. Korolchuk 2007 18057029 Cell line S2 mBSA uptake High content (microscopy) Custom-made library dsRNA Visual inspection np
GR00128-A-1 41839 FBgn0045035 CG6535 DRSC16060 sp none no hit according to not so stringent primary analysis Combinatorial effect with methyl methanesulphonate (1) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Genome-wide dsRNA rp rp Additional information about the primary screen
GR00128-A-2 41839 FBgn0045035 CG6535 DRSC36932 sp none no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00134-A-1 41839 CG6535 tefu 22502 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 41839 CG6535 tefu 22502 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 41839 CG6535 22502 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 41839 CG6535 tefu 22502 0.726415094339622 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 41839 CG6535 22502 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00172-A-1 41839 CG6535, CG6754 tefu, nbs DRSC16060, custom (nbs) np Increased histone H3 phosphorylation with doxorubicin yes G2-M DNA damage checkpoint regulation (1) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Genome-wide dsRNA Mitotic cell number per well > 20
GR00172-A-2 41839 CG6535 tefu DRSC16060 11.98 none yes with bleocin G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 41839 CG6535 tefu DRSC16060 20.66 Increased histone H3 phosphorylation with doxorubicin yes with doxorubicin G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 41839 CG6535 tefu DRSC16060 59.83 Increased histone H3 phosphorylation with etoposide yes with etoposide G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 41839 CG6535 tefu DRSC16060 35.19 Increased histone H3 phosphorylation after X-ray exposure yes X-ray exposure G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00190-A-1 41839 FBgn0045035 tefu 22502 0.323 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00201-A-1 41839 FBgn0045035 tefu CG6535 (UCSF), tefu (Baum_Lab) np none yes Actin organization and cell morphology (1) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line S2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-2 41839 FBgn0045035 tefu CG6535 (UCSF), tefu (Baum_Lab) np none yes Actin organization and cell morphology (2) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line S2R+ alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-3 41839 FBgn0045035 tefu CG6535 (UCSF), tefu (Baum_Lab) np none yes Actin organization and cell morphology (3) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line Kc167 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-4 41839 FBgn0045035 tefu CG6535 (UCSF), tefu (Baum_Lab) np none yes Actin organization and cell morphology (4) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG2-c2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-5 41839 FBgn0045035 tefu CG6535 (UCSF), tefu (Baum_Lab) np none yes Actin organization and cell morphology (5) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG3-c1 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-6 41839 FBgn0045035 tefu CG6535 (UCSF), tefu (Baum_Lab) np none no Actin organization and cell morphology (6) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG3-c2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00202-A 41839 FBgn0038308 tefu np sp none no Vaccinia virus infection A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. Moser et al. 2010 20585561 Cell line DL1 Virally encoded beta-galactosidase protein expression Fluorescence Custom-made Kinases, phosphatases and selected genes dsRNA Z-score < -2 in both duplicates Author-reviewed data
GR00218-S 41839 CG6535 tefu AMB21913 0.75 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 41839 CG6535 6535R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 41839 CG6535 tefu 108074 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 41839 CG6535 tefu 22502 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 41839 CG6535 tefu 108074 0.541378237759934 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 41839 FBgn0045035 tefu DRSC16060 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 41839 CG6535 tefu 108074 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00326-A 41839 FBgn0045035 tefu 108074 np none Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
GR00335-A 41839 FBgn0045035 tefu JF01422 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 41839 FBgn0045035 tefu GL00138 np Embryonic lethal most embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
41839 FBgn0045035 np np sp none
GR00367-S 41839 CG6535 22502 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00370-A 41839 FBgn0045035 tefu -1.03 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00370-A 41839 FBgn0045035 tefu -1.56 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00370-A 41839 FBgn0045035 tefu 0.72 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00379-A-1 41839 CG6535 108074 -0.234935032472605 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 41839 CG6535 GD22502 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.