RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 41885
• Symbol: Act88F
• Description: Actin 88F

DataSource: http://genomernai.dkfz.de/v16/genedetails/41885

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	41885	FBgn0000047	Act88F	DRSC14105	-1.238861718	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	41885	FBgn0000047	Act88F	DRSC14105	-0.033616375	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0	41885	FBgn0000047		DRSC14105	weak	ERK activation increased		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	41885			HFA14105	0.4	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	41885			HFA14105	1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	41885	FBgn0000047	Act88F	DRSC14105	medium	Binucleate cells with microtubule extensions		no
GR00034-A	41885		Actin	np	12	Increased Dipt::lacZ protein expression			without lipopolysaccharide stimulation, CDRi	Imd pathway regulation	Functional dissection of an innate immune response by a genome-wide RNAi screen.	Foley and O'Farrell	2004	15221030	Cell line	S2	Dipt::lacZ protein expression	Colorimetrics	Custom-made	Selected genes	dsRNA	Percentage LacZ-positive cells	Complex criteria	Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish)
GR00034-A	41885		Actin	np	22	Increased Dipt::lacZ protein expression after lipopolysaccharide stimulation			with lipopolysaccharide stimulation, EDRi	Imd pathway regulation	Functional dissection of an innate immune response by a genome-wide RNAi screen.	Foley and O'Farrell	2004	15221030	Cell line	S2	Dipt::lacZ protein expression	Colorimetrics	Custom-made	Selected genes	dsRNA	Percentage LacZ-positive cells	Complex criteria	Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish)
	41885	FBgn0000047	Act88F	LP12170	sp	none		no	library: DGC2
GR00051-A-1	41885	CG5178	Act88F	np	np	Decreased Candida albicans phagocytosis		yes	validated (30 % cells phagocytosing)	Candida albicans phagocytosis (1)	Identification of Drosophila gene products required for phagocytosis of Candida albicans.	Stroschein-Stevenson et al.	2006	16336044	Cell line	S2	C. albicans phagocytosis, actin protein expression and DNA content	Fluorescence	rp	Selected genes	dsRNA	Percentage cells phagocytosing C. albicans	<
GR00051-A-2	41885	CG5178	Act88F	np	24	Decreased Escherichia coli phagocytosis		yes		Candida albicans phagocytosis (2)	Identification of Drosophila gene products required for phagocytosis of Candida albicans.	Stroschein-Stevenson et al.	2006	16336044	Cell line	S2	Escherichia coli phagocytosis, actin protein expression and DNA content	Fluorescence	rp	Selected genes	dsRNA	Percentage cells phagocytosing E. coli	<
GR00051-A-3	41885	CG5178	Act88F	np	26	Decreased latex beads phagocytosis		yes		Candida albicans phagocytosis (3)	Identification of Drosophila gene products required for phagocytosis of Candida albicans.	Stroschein-Stevenson et al.	2006	16336044	Cell line	S2	Latex beads phagocytosis, actin protein expression and DNA content	Fluorescence	rp	Selected genes	dsRNA	Percentage cells phagocytosing latex beads	<
GR00130-A	41885	FBgn0000047	Act88F	np	1.5 - 2	Increased mutant human huntingtin aggregation		no		Mutant human huntingtin aggregation	RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation.	Doumanis et al.	2009	19789644	Cell line	BG2-c2	Nhtt(62Q)EGFP aggregate number and size	Fluorescence	OpenBiosystems RNAi library	Selected genes	dsRNA	Z-score	Suppressor: <
GR00134-A-1	41885	CG5178	Act88F	9779	np	Lethal		yes	embryonic	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	41885	CG5178	Act88F	9780	np	Lethal		yes	embryonic	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-3	41885	CG5178	Act88F	9780	np	Spotty (discontinuous) Z-line		no		Muscle morphogenesis and function (3)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	ZCL0663 protein expression	Fluorescence	np	Selected genes	UAS-IR construct	np	np
GR00135-A-1	41885	CG5178	Act88F	9780	1.16	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	41885	CG5178		9780	0.5	none		no	excluded (potential off targeting effects)	Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00142-A-1	41885	CG5178	Act88F	9780	0.588888888888888	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	41885	CG5178		9780	sp	Completely lethal (before pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	41885	FBgn0000047	Act88F	9780	0.319	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00191-A-1	41885	CG5178	Act88F	np	np	none		yes		Blood cell homeostasis regulation (1)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-2	41885	CG5178	Act88F	np	np	none		no		Blood cell homeostasis regulation (2)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00194-A	41885	CG5178	actin 88F	np	np	none		yes	decision tree - Arp/Scar: top 40	S2 cell spreading	A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis.	D'Ambrosio and Vale	2010	21041442	Cell line	S2U	alpha-tubulin and actin protein expression	Fluorescence	V2 RNAi library	Genome-wide	dsRNA	Complex, sp	Complex criteria
GR00214-A-1	41885	FBgn0000047	Act88F	DRSC14105	-1.68	Decreased ERK phosphorylation after EGF stimulation	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	41885	FBgn0000047	Act88F	DRSC14105	-0.1	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	41885	FBgn0000047	Act88F	DRSC14105	1.35	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	41885	FBgn0000047	Act88F	DRSC14105	1.38	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00216-A	41885	FBgn0000047	Act88F	BKN33062	-0.4	none		no		Wnt proteins secretion	p24 proteins are required for secretion of Wnt ligands.	Buechling et al.	2011	22094269	Cell line	S2R+	Wg pathway reporter	Luminescence	BKN	Cellular transporting machinery genes	dsRNA	Z-score	> 2.5 OR < -2.5	Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00217-A-1	41885	CG5178	actin88F	np	weak	Decreased WgRluc and not changed sFluc protein expression		no		Wg pathway regulation (1)	A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion.	Port et al.	2011	21886182	Cell line	S2R+	WgRluc and sFluc protein expression	Luminescence	np	Genome-wide	dsRNA	Phenotype strength	Complex criteria	Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00218-S	41885	CG5178	Act88F	AMB30269	-1.1	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00238-S-1	41885	CG5178	Act88F	9780	sp	Embryo lethal		yes	library: VDRC	Muscle development and maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	Mef2-GAL4	Climbing ability and viability	Negative geotaxis assay and visual inspection	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-1	41885	CG5178	Act88F	5178R-1	sp	Embryo lethal		yes	library: NIG	Muscle development and maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	Mef2-GAL4	Climbing ability and viability	Negative geotaxis assay and visual inspection	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2	41885	CG5178	Act88F	9780	sp	Climbing defect		no	library: VDRC	Muscle maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	TARGET Mef2-GAL4	Climbing ability	Negative geotaxis assay	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1	41885	CG5178		5178R	np	Decreased Chp protein expression		no	not detectable Chp level	Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00284-A	41885	CG5178	Act88F	9780	-0.6402806647324517	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	41885	FBgn0000047	Act88F	DRSC14105	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
	41885	FBgn0000047	np	np	sp	none
GR00379-A-1	41885	CG5178		9780	-0.16949684881221716	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	41885	CG5178		GD9780		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.