RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 41911
  • Symbol: nsl1
  • Description: non-specific lethal 1
DataSource: http://genomernai.dkfz.de/v16/genedetails/41911

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 41911 FBgn0038364 CG4699 DRSC15625 0.144818184 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 41911 FBgn0038364 CG4699 DRSC15625 -1.707859909 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1 41911 HFA15625 2.3 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 41911 HFA15625 1.6 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00036-A 41911 CG4699 CG4699 DRSC15625 moderate Increased GFP protein expression yes increased intracellular infection Intracellular Listeria monocytogenes infection Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Agaisse et al. 2005 16020693 Cell line SL2 GFP protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np
GR00040-A-0 41911 FBgn0038364 DRSC15625 17.3 Increased Wnt reporter activity no Wnt signaling Functional genomic analysis of the Wnt-wingless signaling pathway. DasGupta et al. 2005 15817814 Cell line Clone 8 Wnt signaling Dual luciferase DRSC dsRNA Z-score >
GR00045-A-0 41911 FBgn0038364 DRSC15625 19.79 Increased Hedgehog reporter activity no Hedgehog signaling A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken et al. 2005 16311596 Cell line Clone 8 Hedgehog signaling Dual luciferase DRSC dsRNA Z-score >
GR00047-A-1 41911 FBgn0038364 CG4699 DRSC15625 sp Decreased horseradish peroxidase protein expression yes Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
GR00047-A-2 41911 FBgn0038364 CG4699 np np Fused Golgi membranes with endoplasmic reticulum no Constitutive protein secretion and Golgi organization (2) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Mouse Mannosidase II protein expression Fluorescence Selected genes dsRNA Visual inspection Golgi membrane depletion > 50 % of the cells
41911 FBgn0038364 CG4699 np sp none no library: DGC1
41911 FBgn0038364 CG4699 LP09056 sp none no library: DGC1
GR00052-A 41911 CG4699 DRSC15625 np Decreased Ca2+ uptake after thapsigargin stimulation no Store-operated calcium influx regulation Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity. Zhang et al. 2006 16751269 Cell line S2 Ca2+ uptake Fluorescence np Genome-wide dsRNA Z-score < -3
GR00157-A 41911 CG4699 DRSC31788 0.58 Upregulation of Wg pathway no Wg pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA log ratio > 30 % difference from control
GR00157-A 41911 CG4699 DRSC31789 0.59 Upregulation of Wg pathway no Wg pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA log ratio > 30 % difference from control
GR00170-A-1 41911 NSL1 DRSC15625 -5.2 Decreased HIF dependent transcription with desferrioxamine yes HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00170-A-2 41911 NSL1 DRSC31789 74.5 Decreased HIF dependent transcription with desferrioxamine yes group A HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-2 41911 NSL1 DRSC31788 84.4 Decreased HIF dependent transcription with desferrioxamine yes group A HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-3 41911 NSL1 DRSC31789 63 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00170-A-3 41911 NSL1 DRSC31788 62.8 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00172-A-1 41911 CG4699, CG6754 CG4699, nbs DRSC15625, custom (nbs) np Increased histone H3 phosphorylation with doxorubicin yes G2-M DNA damage checkpoint regulation (1) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Genome-wide dsRNA Mitotic cell number per well > 20
GR00172-A-2 41911 CG4699 CG4699 DRSC15625 17.95 Increased histone H3 phosphorylation with bleocin yes with bleocin G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 41911 CG4699 CG4699 DRSC15625 35.19 Increased histone H3 phosphorylation with doxorubicin yes with doxorubicin G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 41911 CG4699 CG4699 DRSC15625 50.43 Increased histone H3 phosphorylation with etoposide yes with etoposide G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 41911 CG4699 CG4699 DRSC15625 49.11 Increased histone H3 phosphorylation after X-ray exposure yes X-ray exposure G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00175-A 41911 CG4699 CG4699 DRSC31788 0.173332487823114 none no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00175-A 41911 CG4699 CG4699 DRSC31789 2.38121986308463 Upregulation of Hh pathway no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00214-A-1 41911 FBgn0262527 wah DRSC15625 -0.61 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 41911 FBgn0262527 wah DRSC15625 -7.11 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 41911 FBgn0262527 wah DRSC15625 -1.86 Decreased ERK phosphorylation after sSpitz and EGF stimulation 10 minutes sSpitz stimulation yes RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 41911 FBgn0262527 wah DRSC15625 -0.12 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00217-A-1 41911 CG4699 CG4699 np strong Decreased WgRluc and not changed sFluc protein expression no Wg pathway regulation (1) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Cell line S2R+ WgRluc and sFluc protein expression Luminescence np Genome-wide dsRNA Phenotype strength Complex criteria Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00218-S 41911 CG4699 wah AMB22275 1.94 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 41911 CG4699 4699R np Lethal no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00273-A-1 41911 FBgn0262527 wah DRSC15625 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00311-A 41911 FBgn0038364 CG4699 DRSC15625 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
41911 np np HFA15625 0.3 none fold change: 0.86
41911 FBgn0262527 np np -4.8 Increased transposon expression fold change: 7.86
41911 FBgn0262527 np np sp none