Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 42515
Symbol: SNF4Agamma
Description: SNF4/AMP-activated protein kinase gamma subunit

DataSource: http://genomernai.dkfz.de/v16/genedetails/42515

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC13461	0.563707276	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC13461	0.846029689	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC13463	-1.837622478	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC13463	1.237991212	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC15879	-1.622352509	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC15879	-2.357430207	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC16847	1.979294421	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42515	FBgn0025803	SNF4Agamma	DRSC16847	0.306583651	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00030-A-2	42515	CG17299	SNF4Agamma	SNF4Agamma	np	none		no		Cell cycle regulation (2)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression	Flow cytometry	rp	Kinases and kinase regulators	dsRNA	np	np
GR00031-A-1	42515			HFA15879	0.1	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-1	42515			HFA13461	0.3	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-1	42515			HFA16847	-2.4	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42515			HFA15879	1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42515			HFA16847	-2.4	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42515			HFA13461	0.1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	42515	FBgn0025803	SNF4Agamma	RE12750	sp	Decreased G1 DNA content		yes	library: DGC2
	42515	FBgn0025803	SNF4Agamma	RE22690	sp	none		yes	library: DGC2
	42515	FBgn0025803	SNF4Agamma	RE59472	sp	none		yes	library: DGC2
	42515	FBgn0025803	SNF4Agamma	np	sp	none		yes	library: DGC1
	42515	FBgn0025803	SNF4Agamma	LD22662	sp	none		yes	library: DGC1
	42515	FBgn0025803, FBgn0041605	SNF4Agamma, cpx	np	sp	none		no	library: DGC1
	42515	FBgn0025803, FBgn0041605	SNF4Agamma, cpx	GH01416	sp	none		no	library: DGC1
GR00048-A-2	42515	FBgn0025803	SNF4Agamma	np	1.236	none		yes		Cell size and cell-cycle regulation (2)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Proliferation and viability	Colorimetrics		Selected genes	dsRNA	MTS metabolic activity	> 2.5 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3	42515	FBgn0025803	SNF4Agamma	np	461	none		yes		Cell size and cell-cycle regulation (3)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Cell number	Fluorescence		Selected genes	dsRNA	Average cell number per field	> 2 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-4	42515	FBgn0025803	SNF4Agamma	np	below threshold	none		no		Cell size and cell-cycle regulation (4)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Number of mitotic cells	Fluorescence		Selected genes	dsRNA	Percentage	Visual inspection	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00128-A-1	42515	FBgn0025803	CG17299	DRSC16847	sp	none		no	hit according to not so stringent primary analysis	Combinatorial effect with methyl methanesulphonate (1)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Genome-wide	dsRNA	rp	rp	Additional information about the primary screen
GR00128-A-2	42515	FBgn0025803	CG17299	DRSC37038	sp	none		no		Combinatorial effect with methyl methanesulphonate (2)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Selected genes	dsRNA	rp	rp
GR00148-A	42515	FBgn0025803	SNF4Agamma	np	3.62	Increased P-JNK protein expression with PGN	60 min PGN induction	no		PGN-induced dJNK phosphorylation	A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling.	Bond and Foley	2009	19893628	Cell line	S2	P-JNK protein expression	Fluorescence	Custom-made	Genome-wide	dsRNA	Z-score	Complex criteria	Only hits stored in GenomeRNAi
GR00169-A	42515	FBgn0025803	SNF4Agamma	DRSC16847	-3.4	Decreased dAkt phosphorylation after insulin stimulation	10 min insulin stimulation	no		Akt-TOR pathway negative feedback regulation	Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling.	Kockel et al.	2010	20585550	Cell line	Kc167	dAkt phosphorylation	Fluorescence	Custom-made	Genome-wide	dsRNA	Z-score	< -2.5 OR > 2.5
GR00172-A-1	42515		SNF4Agamma, nbs	DRSC16847, custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		no	excluded from further analysis: role in mitosis	G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00202-A	42515	FBgn0025803	SNF4Agamma	np	sp	Decreased number of vaccinia virus infected cells		yes	validated: non-overlapping dsRNAs used	Vaccinia virus infection	A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.	Moser et al.	2010	20585561	Cell line	DL1	Virally encoded beta-galactosidase protein expression	Fluorescence	Custom-made	Kinases, phosphatases and selected genes	dsRNA	Z-score	< -2 in both duplicates	Author-reviewed data
GR00214-A-1	42515	FBgn0025803	SNF4Agamma	DRSC13461	-0.66	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	42515	FBgn0025803	SNF4Agamma	DRSC13461	-1.62	Decreased ERK phosphorylation after EGF stimulation	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42515	FBgn0025803	SNF4Agamma	DRSC13461	1.71	Increased ERK phosphorylation after sSpitz and EGF stimulation	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42515	FBgn0025803	SNF4Agamma	DRSC13461	0.86	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	42515	CG17299	SNF4Agamma	AMB34612	1.15	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	42515	CG17299		17299R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00296-A	42515		SNF4Ag	np	-2.1	Decreased Sindbis virus (SINV) infection			prim. screen rep. B: -2.27; sec. val. screen: -7.14	Sindbis virus (SINV) infection	Genome-wide RNAi Screen Identifies SEC61A and VCP as Conserved Regulators of Sindbis Virus Entry	Panda et al.	2013	24332855	Cell line	DL1	Sindbis virus (SINV) GFP protein expression	Fluorescence	Ambion	Genome-wide	dsRNA	Z-score	< -2 OR > 2	Primary screen performed in duplicate. Z-scores from replicate B and from secondary validation screen are shown in the comment field. Recombinant SINV (HRsp) expressing GFP was used for infection.
GR00311-A	42515	FBgn0025803	SNF4Agamma	DRSC13461	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00311-A	42515	FBgn0025803	SNF4Agamma	DRSC15879	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00311-A	42515	FBgn0025803	SNF4Agamma	DRSC16847	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	42515	FBgn0264357	SNF4Agamma	JF02060	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	42515	FBgn0264357	SNF4Agamma	HMS01205	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	42515	FBgn0264357	SNF4Agamma	GL00252	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	42515	FBgn0025803	np	np	sp	none
GR00367-S	42515	CG17299		26291	np	liquid clearance defect			Trip	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00370-A	42515	FBgn0025803	SNF4Agamma		0.15	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	42515	FBgn0025803	SNF4Agamma		1.75	Increased CncC (Nrf2) reporter expression				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	42515	FBgn0025803	SNF4Agamma		0.84	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00388-A	42515	CG17299		BL34726		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	42515	CG17299		BL26291		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.