Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 42803
Symbol: Hmgcr
Description: HMG Coenzyme A reductase

DataSource: http://genomernai.dkfz.de/v16/genedetails/42803

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	42803	FBgn0001205	Hmgcr	DRSC16704	1.230681052	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42803	FBgn0001205	Hmgcr	DRSC16704	0.226723453	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0	42803	FBgn0001205		DRSC16704	weak	ERK activation reduced		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	42803			HFA16704	0.3	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42803			HFA16704	1.5	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00036-A	42803	CG10367	Hmgcr	DRSC16704	moderate	Decreased GFP protein expression		yes	decreased intracellular infection	Intracellular Listeria monocytogenes infection	Genome-wide RNAi screen for host factors required for intracellular bacterial infection.	Agaisse et al.	2005	16020693	Cell line	SL2	GFP protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np
GR00037-A-1	42803		Hmgcr	np	> 4	Downregulation of JAK/STAT pathway		yes		JAK/STAT pathway regulation (1)	Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila.	Baeg et al.	2005	16055650	Cell line	S2-NP	JAK/STAT pathway reporter	Luminescence	rp	Genome-wide	dsRNA	Fold change of relative luciferase units	Complex criteria
GR00037-A-2	42803		Hmgcr	np	>2	Downregulation of JAK/STAT pathway after Upd stimulation		yes		JAK/STAT pathway regulation (2)	Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila.	Baeg et al.	2005	16055650	Cell line	S2-NP	JAK/STAT pathway reporter	Luminescence	rp	Selected genes	dsRNA	Fold change of relative luciferase units	np
GR00037-A-3	42803		Hmgcr	np	np	none		no	with Upd stimulation	JAK/STAT pathway regulation (3)	Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila.	Baeg et al.	2005	16055650	Cell line	S2-NP	Stat92E tyrosine phosphorylation	Immunoblotting	rp	Selected genes	dsRNA	Visual inspection	np
GR00046-A-1	42803	FBgn0001205	Hmgcr	DRSC16704	8.1	Decreased M. fortuitum intracellular infection	measurement after 3 days	yes		Mycobacterial infection (1) - M. fortuitum infection	Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection.	Philips et al.	2005	16020694	Cell line	S2	map24::GFP and map49::GFP protein expression	Fluorescence	np	Genome-wide	dsRNA	Z-score	>
GR00046-A-2	42803	FBgn0001205	Hmgcr	DRSC16704	np	Decreased E. coli phagocytosis		no		Mycobacterial infection (2) - E. coli phagocytosis	Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection.	Philips et al.	2005	16020694	Cell line	S2	Intracellular FITC-E. coli uptake	Fluorescence	np	Selected genes	dsRNA	p-value	< 0.01
GR00047-A-1	42803	FBgn0001205	Hmgcr	DRSC16704	sp	Decreased horseradish peroxidase protein expression		no		Constitutive protein secretion and Golgi organization (1)	Functional genomics reveals genes involved in protein secretion and Golgi organization.	Bard et al.	2006	16452979	Cell line	S2	Horseradish peroxidase protein expression	Luminescence		Genome-wide	dsRNA	Z-score	< -1.5
	42803	FBgn0001205, FBgn0000179	Hmgcr, bi	LD15354	sp	none		no	library: DGC1
GR00052-A	42803		Hmgcr	DRSC16704	np	Decreased Ca2+ uptake after thapsigargin stimulation		no		Store-operated calcium influx regulation	Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity.	Zhang et al.	2006	16751269	Cell line	S2	Ca2+ uptake	Fluorescence	np	Genome-wide	dsRNA	Z-score	< -3
GR00062-A	42803	FBgn0001205	Hmgcr	np	4	Decreased Ca2+ influx				Ca2+ channel regulation	CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.	Vig et al.	2006	16645049	Cell line	S2R+	Ca2+ influx	Fluorescence	np	Genome-wide	dsRNA	Complex, sp	>
GR00083-A	42803	CG10367	Hmgcr	np	12.83	Decreased Brucella melitensis infection		no		Brucella melitensis infection	RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication.	Qin et al.	2008	18654626	Cell line	S2	Intracellular bacterial GFP protein expression	Fluorescence	Drosophila RNAi Library Release 1.0	Selected genes	dsRNA	Relative infection	> 2 standard deviations below untreated control	Additional information about secondary screens (gentamicin protection assays)
GR00086-A-0	42803	FBgn0001205		DRSC16704	-3.08/-0.36	Decreased Influenza replication		no		Influenza replication	Drosophila RNAi screen identifies host genes important for influenza virus replication.	Hao et al.	2008	18615016	Cell line	DL1	Influenza replication	Luciferase	Ambion/DRSC		dsRNA	Z-score	<
GR00130-A	42803	FBgn0001205	Hmgcr	np	<= -2	Decreased mutant human huntingtin aggregation		no		Mutant human huntingtin aggregation	RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation.	Doumanis et al.	2009	19789644	Cell line	BG2-c2	Nhtt(62Q)EGFP aggregate number and size	Fluorescence	OpenBiosystems RNAi library	Selected genes	dsRNA	Z-score	Suppressor: <
GR00194-A	42803	CG10367	Hmgcr	np	np	none		yes	actin/area: top 40, FFT: bottom 40, No. of corners: bottom 40, decision tree - cofilin/CAP: top 40	S2 cell spreading	A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis.	D'Ambrosio and Vale	2010	21041442	Cell line	S2U	alpha-tubulin and actin protein expression	Fluorescence	V2 RNAi library	Genome-wide	dsRNA	Complex, sp	Complex criteria
GR00214-A-1	42803	FBgn0001205	Hmgcr	DRSC16704	-1.79	Decreased ERK phosphorylation after EGF stimulation	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	42803	FBgn0001205	Hmgcr	DRSC16704	-3.19	Decreased ERK phosphorylation after EGF stimulation	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42803	FBgn0001205	Hmgcr	DRSC16704	-1.99	Decreased ERK phosphorylation after sSpitz and EGF stimulation	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42803	FBgn0001205	Hmgcr	DRSC16704	-0.29	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	42803	CG10367	Hmgcr	AMB26544	-0.79	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	42803	CG10367		10367R	sp	Decreased alpha1,3-fucose Chp glycosylation, decreased alpha1,6-fucose Chp glycosylation, decreased mannose Chp glycosylation		yes		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00239-A-2	42803	CG10367		10367R	np	none		no		Glycosylation regulation (2)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation	Luminescence	np	Selected genes	UAS-IR construct	p-value	< 0.005
GR00245-A	42803	FBgn0001205	Hmgcr	DRSC16704	np	Decreased formation of mutant Huntingtin (Httex1-Qn) protein aggregates		yes	validated	Huntingtin (Htt) aggregates formation	A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila.	Zhang et al.	2010	20100940	Cell line	S2	Mutant Httex1-Qn-eGFP protein expression and DNA content	Fluorescence	Version 1 and np	Genome-wide	dsRNA	Aggregate number, size, and signal intensity	> 2 standard deviations	Additional information about secondary screens (reproducibility and non-overlaping dsRNAs)
GR00282-A	42803	CG10367	Hmgcr	108617	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	42803	CG10367	Hmgcr	108617	0.5047895162831131	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	42803	FBgn0001205	Hmgcr	DRSC16704	np	Actin defect, other cellular phenotype			cell shape variable, decreased level of actin, decreased cell number, loss of cell monolayer	Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00314-A	42803	FBgn0263782	Hmgcr	RNA004986	18.25	Decreased MAPK phosphorylation			hit in validation screens	RAS-related MAPK activation	A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila	Ashton-Beaucage et al.	2014	24643257	Cell line	S2	MAPK phosphorylation	Fluorescence	Custom-made	Genome-wide	dsRNA	pMAPK signal (%GFP dsRNA)	np	S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai
GR00314-A	42803	FBgn0263782	Hmgcr	RNA007351	24.03	Decreased MAPK phosphorylation			hit in validation screens	RAS-related MAPK activation	A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila	Ashton-Beaucage et al.	2014	24643257	Cell line	S2	MAPK phosphorylation	Fluorescence	Custom-made	Genome-wide	dsRNA	pMAPK signal (%GFP dsRNA)	np	S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai
	42803	FBgn0001205	np	np	sp	none
GR00367-S	42803	CG10367		108617	np	lethal or adult morphology phenotype			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	42803	CG10367		108617	-0.32910052130647116	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	42803	CG10367		KK108617		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.