Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 42845
Symbol: Miro
Description: Mitochondrial Rho

DataSource: http://genomernai.dkfz.de/v16/genedetails/42845

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	42845	FBgn0039140	Miro	DRSC15795	-0.506850354	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42845	FBgn0039140	Miro	DRSC15795	-1.381458265	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00012-A-1	42845	CG5410	dMiro	np	np	Increased viability with doxorubicin	yes		Caspase activation in apoptosis (1) - combinatorial effect with doxorubicin	A genome-wide RNAi screen reveals multiple regulators of caspase activation.	Yi et al.	2007	17998402	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	HFA	Genome-wide	dsRNA	Z-score	Complex criteria
GR00012-A-2	42845	CG5410	dMiro	np	np	Decreased caspase-3/7-like activity with doxorubicin	yes		Caspase activation in apoptosis (2) - combinatorial effect with doxorubicin	A genome-wide RNAi screen reveals multiple regulators of caspase activation.	Yi et al.	2007	17998402	Cell line	Kc167	Caspase 3/7 activity	Luminescence	HFA	Selected genes	dsRNA	p-value	< 0.05
GR00012-A-3	42845	CG12284, CG5410	diap1, dMiro	np	np	Increased viability with diap1 RNAi	yes		Caspase activation in apoptosis (3) - combinatorial effect with diap1 RNAi	A genome-wide RNAi screen reveals multiple regulators of caspase activation.	Yi et al.	2007	17998402	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	HFA	Selected genes	dsRNA	p-value	< 0.05
GR00031-A-1	42845			HFA15795	0	none	yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42845			HFA15795	-0.7	none	no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	42845	FBgn0039140	CG5410	LD24810	sp	none	no	library: DGC1
GR00128-A-1	42845	FBgn0039140	CG5410	DRSC15795	sp	none	no	hit according to not so stringent primary analysis	Combinatorial effect with methyl methanesulphonate (1)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Genome-wide	dsRNA	rp	rp	Additional information about the primary screen
GR00134-A-1	42845	CG5410	Miro	6851	np	none	no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	42845	CG5410	Miro	6852	np	none	no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00142-A-1	42845	CG5410	Miro	6851	0.592592592592592	none	no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	42845	CG5410		6852	0	none	no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	42845	CG5410		6851	0	none	no	partially lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	42845	FBgn0039140	Miro	6851	-0.549	none	no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00218-S	42845	CG5410		AMB18605	0.2	none	no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00238-S-1	42845	CG5410	Miro	106683	sp	Pupal lethal	yes	library: VDRC	Muscle development and maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	Mef2-GAL4	Climbing ability and viability	Negative geotaxis assay and visual inspection	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2	42845	CG5410	Miro	106683	sp	none	no	library: VDRC	Muscle maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	TARGET Mef2-GAL4	Climbing ability	Negative geotaxis assay	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1	42845	CG5410		5410R	sp	none	no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	42845	CG5410	Miro	106683	np	none			piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	42845	CG5410	Miro	106683	0.3088507593448968	none			piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	42845	FBgn0039140	Miro	DRSC15795	np	none			Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	42845	CG5410	Miro	106683	np	none			Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00326-A	42845	FBgn0039140	Miro	106683	np	none			Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
	42845	FBgn0039140	np	np	sp	none
GR00367-S	42845	CG5410		106683	np	none		VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	42845	CG5410		106683	-0.3524838684059447	none		No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	42845	CG5410		GD6851		none			Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	42845	CG5410		BL27695		none			Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	42845	CG5410		KK106683		none			Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.