Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 42862
Symbol: Tsc1
Description: Tsc1

DataSource: http://genomernai.dkfz.de/v16/genedetails/42862

Export (tab separated) Export to Excel

Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00001-A	42862	FBgn0026317	Tsc1	DRSC16890	-0.1	none		yes		Cell size regulation	PDGF/VEGF signaling controls cell size in Drosophila.	Sims et al.	2009	19216764	Cell line	S2R+	F-actin and microtubule protein expression and DNA content	Fluorescence	rp	Genome-wide	dsRNA	Cell area Z-score	< -2
GR00002-A	42862	FBgn0026317	Tsc1	DRSC16890	-2.444622082	Decreased lipid droplet area		yes		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42862	FBgn0026317	Tsc1	DRSC16890	-1.965462032	Decreased lipid droplet area		yes		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00013-A	42862	CG6147	Tsc1	np	np	Decreased Chlamydia caviae infection		yes		Chlamydia caviae infection	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.	Derre et al.	2007	17967059	Cell line	SL2	Chlamydia MOMP and LPS expression and DNA content	Fluorescence	np	Genome-wide	dsRNA	Visual inspection	np	Additional information about secondary screens
GR00031-A-1	42862			HFA16890	0.2	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42862			HFA16890	0.5	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	42862	FBgn0026317	Tsc1	LD23779	sp	Decreased G1 DNA content, increased cytokinesis		yes	library: DGC1
GR00048-A-2	42862	FBgn0026317	Tsc1	np	1.307	none		yes		Cell size and cell-cycle regulation (2)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Proliferation and viability	Colorimetrics		Selected genes	dsRNA	MTS metabolic activity	> 2.5 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3	42862	FBgn0026317	Tsc1	np	449	none		yes		Cell size and cell-cycle regulation (3)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Cell number	Fluorescence		Selected genes	dsRNA	Average cell number per field	> 2 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-4	42862	FBgn0026317	Tsc1	np	0.9	Increased number of mitotic cells		no		Cell size and cell-cycle regulation (4)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	BjÓ§rklund et al.	2006	16496002	Cell line	S2	Number of mitotic cells	Fluorescence		Selected genes	dsRNA	Percentage	Visual inspection	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00088-A	42862	CG6147	Tsc1	np	np	GFP protein expression under hypoxia				Translational activity under hypoxia	An RNA interference screen identifies a novel regulator of target of rapamycin that mediates hypoxia suppression of translation in Drosophila S2 cells.	Lee et al.	2008	18653470	Cell line	S2	GFP protein expression	Western blot	Custom-made	Selected genes	dsRNA	np	np
GR00128-A-1	42862	FBgn0026317	CG6147	DRSC16890	sp	none		no	hit according to not so stringent primary analysis	Combinatorial effect with methyl methanesulphonate (1)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Genome-wide	dsRNA	rp	rp	Additional information about the primary screen
GR00128-A-2	42862	FBgn0026317	CG6147	DRSC27643	sp	Synthetic lethal with methyl methanesulphonate		no		Combinatorial effect with methyl methanesulphonate (2)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Selected genes	dsRNA	rp	rp
GR00134-A-1	42862	CG6147	Tsc1	22252	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	42862	CG6147	Tsc1	22252	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies Î±2Î´3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	42862	CG6147		22252	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCÎ”4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00142-A-1	42862	CG6147	Tsc1	22252	0.716666666666666	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	42862	CG6147		22252	sp	Gain of bristles, notum malformation migration		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00152-A	42862	CG6147	Tsc1	np	0.79676	none		no	N(delta)ecn Notch stimulation; con-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	42862	CG6147	Tsc1	np	2.8669	Upregulation of Notch pathway after Notch stimulation		no	N(delta)ecn Notch stimulation; m3-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	42862	CG6147	Tsc1	np	-1.6425	none		no	without Notch stimulation	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00169-A	42862	FBgn0026317	Tsc1	DRSC16890	-4.4	Decreased dAkt phosphorylation after insulin stimulation	10 min insulin stimulation	yes		Akt-TOR pathway negative feedback regulation	Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling.	Kockel et al.	2010	20585550	Cell line	Kc167	dAkt phosphorylation	Fluorescence	Custom-made	Genome-wide	dsRNA	Z-score	< -2.5 OR > 2.5
GR00190-A-1	42862	FBgn0026317	Tsc1	22252	0.2	Increased triglyceride expression		yes		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00190-A-2	42862	FBgn0026317	Tsc1	22252	-0.295	Decreased triglyceride expression		no		Adiposity regulation (2)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	nsyb-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-3	42862	FBgn0026317	Tsc1	22252	-0.007	none		no		Adiposity regulation (3)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	C57-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-4	42862	FBgn0026317	Tsc1	22252	-0.011	none		no		Adiposity regulation (4)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	oe-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-5	42862	FBgn0026317	Tsc1	22252	-0.544	Decreased triglyceride expression		no		Adiposity regulation (5)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	ppl-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00218-S	42862	CG6147		AMB23022	-0.5	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	42862	CG6147		6147R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	42862	CG6147	Tsc1	110811	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	42862	CG6147	Tsc1	22252	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	42862	CG6147	Tsc1	22252	0.8324705201438476	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	42862	FBgn0026317	Tsc1	DRSC16890	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	42862	FBgn0026317	Tsc1	JF01484	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	42862	FBgn0026317	Tsc1	JF01262	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	42862	FBgn0026317	np	np	sp	none
GR00367-S	42862	CG6147		22252	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	42862	CG6147		22252	-0.3442777295238705	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	42862	CG6147		GD22252		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.