Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 42952
Symbol: Ets96B
Description: Ets96B

DataSource: http://genomernai.dkfz.de/v16/genedetails/42952

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	42952	FBgn0039225	Ets96B	DRSC16127	0.309705331	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42952	FBgn0039225	Ets96B	DRSC16127	1.201627459	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00004-A-0	42952		Ets96B	DRSC16127	0.64	Endo-siRNA reporter downregulated		no		Drosophila small RNA pathways	Comparative analysis of argonaute-dependent small RNA pathways in Drosophila.	Zhou et al.	2008	19026789	Cell line	S2	miRNA and siRNA pathway activity	Dual luciferase	DRSC		dsRNA	Fold change endo-siRNA reporter	1.5/0.6
GR00004-A-0	42952		Ets96B	DRSC16127	0.79	siRNA reporter downregulated		no		Drosophila small RNA pathways	Comparative analysis of argonaute-dependent small RNA pathways in Drosophila.	Zhou et al.	2008	19026789	Cell line	S2	miRNA and siRNA pathway activity	Dual luciferase	DRSC		dsRNA	Fold change endo-siRNA reporter	1.5/0.6
GR00021-A-0	42952	FBgn0039225		DRSC16127	weak	ERK activation increased		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	42952			HFA16127	0.1	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42952			HFA16127	-0.5	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	42952	FBgn0039225, FBgn0039226	Ets96B, CG18410	SD17695	sp	none		no	library: DGC2
	42952	FBgn0039225, FBgn0032233	Ets96B, dpr19	RH09633	sp	none		no	library: DGC2
GR00065-A	42952	CG6892	Ets96B	Ets96B	np	none		no		Dendrite pattern formation	Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites.	Parrish et al.	2006	16547170	Tissue	GAL4221	mCD8 protein expression	Fluorescence	Custom-made	Transcription factors	UAS-IR construct	np	Phenotypes in multiple blind tests	Additional information about secondary screens
GR00135-A-1	42952	CG6892	Ets96B	30552	0.77	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies Î±2Î´3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00142-A-1	42952	CG6892	Ets96B	30552	0.362745098039215	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	42952	CG6892		30552	sp	Completely lethal (pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	42952	FBgn0039225	Ets96B	30552	2.215	Increased triglyceride expression		yes		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00190-A-2	42952	FBgn0039225	Ets96B	30552	-0.082	none		no		Adiposity regulation (2)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	nsyb-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-3	42952	FBgn0039225	Ets96B	30552	-0.299	Decreased triglyceride expression		no		Adiposity regulation (3)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	C57-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-4	42952	FBgn0039225	Ets96B	30552	-0.332	Decreased triglyceride expression		no		Adiposity regulation (4)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	oe-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00190-A-5	42952	FBgn0039225	Ets96B	30552	-0.4	Decreased triglyceride expression		no		Adiposity regulation (5)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Tissue	ppl-GAL4	Total fly triglyceride expression	Colorimetrics	np	Selected genes	UAS-IR construct	Triglyceride change	> 0.25 OR <-0.25 (change >25%)	Additional information about the primary screen
GR00214-A-1	42952	FBgn0039225	Ets96B	DRSC16127	-0.08	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	42952	FBgn0039225	Ets96B	DRSC16127	-0	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42952	FBgn0039225	Ets96B	DRSC16127	1.68	Increased ERK phosphorylation after sSpitz and EGF stimulation	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42952	FBgn0039225	Ets96B	DRSC16127	0.8	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	42952	CG6892		AMB21118	-0.47	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00284-A	42952	CG6892	Ets96B	30552	-0.3703169047402626	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	42952	FBgn0039225	Ets96B	DRSC16127	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	42952	FBgn0039225	Ets96B	HMS00092	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	42952	FBgn0039225	np	np	sp	none
	42952	np	np	HFA16127	-0.43	none			fold change: 1.19
GR00367-S	42952	CG6892		30552	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	42952	CG6892		30552	-0.20692447897230165	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	42952	CG6892		GD30552		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	42952	CG6892		BL34783		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.