| GR00002-A |
42964 |
FBgn0039234 |
nct |
DRSC16172 |
-1.035487515 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
42964 |
FBgn0039234 |
nct |
DRSC16172 |
-0.012603228 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00031-A-1 |
42964 |
|
|
HFA16172 |
-0.1 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
42964 |
|
|
HFA16172 |
-1.1 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
42964 |
FBgn0039234 |
nct |
LD36812 |
sp |
none |
|
no |
library: DGC1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00128-A-1 |
42964 |
FBgn0039234 |
CG7012 |
DRSC16172 |
sp |
Synthetic lethal with methyl methanesulphonate |
|
no |
|
Combinatorial effect with methyl methanesulphonate (1) |
A network of conserved damage survival pathways revealed by a genomic RNAi screen. |
Ravi et al. |
2009 |
19543366 |
Cell line |
Kc167 |
Viability (synthetic lethal) |
Luminescence |
Version 1 |
Genome-wide |
dsRNA |
rp |
rp |
Additional information about the primary screen |
GR00144-A-1 |
42964 |
CG7012 |
nct |
3D8 T2 |
no phenotype |
none |
temperature: 29 |
yes |
|
Notch pathway regulation (1) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
sca-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-1 |
42964 |
CG7012 |
nct |
3D8 T1 |
no phenotype |
none |
temperature: 29 |
yes |
|
Notch pathway regulation (1) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
sca-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-2 |
42964 |
CG7012 |
nct |
3D8 T2 |
no phenotype |
none |
temperature: 29 |
yes |
|
Notch pathway regulation (2) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
fz3-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-2 |
42964 |
CG7012 |
nct |
3D8 T1 |
no phenotype |
none |
temperature: 29 |
yes |
|
Notch pathway regulation (2) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
fz3-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-3 |
42964 |
CG7012 |
nct |
3D8 T1 |
no phenotype |
none |
temperature: 29 |
no |
|
Notch pathway regulation (3) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-3 |
42964 |
CG7012 |
nct |
3D8 T2 |
no phenotype |
none |
temperature: 29 |
no |
|
Notch pathway regulation (3) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-1 |
42964 |
FBgn0039234 |
nct |
BKN20273 |
np |
Downregulation of Notch pathway |
|
yes |
|
Notch pathway regulation (1) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Cell line |
S2 |
Notch pathway reporter |
Luminescence |
BKN |
Genome-wide |
dsRNA |
Complex, sp |
Complex criteria |
|
GR00189-A-2 |
42964 |
FBgn0039234 |
Nct |
np |
sp |
Downregulation of Notch pathway; adult wing veins: broader |
|
no |
en-GAL4 |
Notch pathway regulation (2) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
en-GAL4, C96-GAL4 and vg-GAL4 |
Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability |
Fluorescence and visual inspection |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00218-S |
42964 |
CG7012 |
|
AMB34109 |
-1.03 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00311-A |
42964 |
FBgn0039234 |
nct |
DRSC16172 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00335-A |
42964 |
FBgn0039234 |
nct |
JF02648 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
|
42964 |
FBgn0039234 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00388-A |
42964 |
CG7012 |
|
BL27498 |
|
Lethal |
|
|
excess of ISC or EB |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |