RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 43506
• Symbol: IntS11
• Description: Integrator 11

DataSource: http://genomernai.dkfz.de/v16/genedetails/43506

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	43506	FBgn0039691	CG1972	DRSC15407	-0.21890561	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	43506	FBgn0039691	CG1972	DRSC15407	-0.68791368	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	43506			HFA15407	-0.9	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	43506			HFA15407	0.1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	43506	FBgn0039691	CG1972	LD08814	sp	none		no	library: DGC1
GR00092-A	43506	FBgn0039691	CG1972	RDM1190-7300201	np	none		no		Microtubule assembly and organization	Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin.	Ducat et al.	2008	18463163	Cell line	S2	DNA content, alpha-tubulin and CP309 protein expression	Fluorescence	OpenBiosystems Drosophila RNAi Library 1.0	Mitotic regulator genes	dsRNA	Complex, sp	Complex criteria
GR00130-A	43506	FBgn0039691	CG1972	np	1.5 - 2	Increased mutant human huntingtin aggregation		no		Mutant human huntingtin aggregation	RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation.	Doumanis et al.	2009	19789644	Cell line	BG2-c2	Nhtt(62Q)EGFP aggregate number and size	Fluorescence	OpenBiosystems RNAi library	Selected genes	dsRNA	Z-score	Suppressor: <
GR00134-A-1	43506	CG1972	CG1972	33450	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	43506	CG1972	CG1972	33451	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	43506	CG1972	CG1972	33450	0.02	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	43506	CG1972		33450	1	none		yes		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00138-A-2	43506	CG1972		33450	8.56	Decreased viability		no		Heart development and function (2)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00142-A-1	43506	CG1972	CG1972	33450	0.58955223880597	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	43506	CG1972		33450	sp	Bristle morphology defects, notum malformation death		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	43506	CG1972		33451	sp	Bristle morphology defects, notum malformation migration		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00152-A	43506	CG1972	CG1972	np	2.5367	none		no	without Notch stimulation	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	43506	CG1972	CG1972	np	-0.85452	none		no	N(delta)ecn Notch stimulation; con-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	43506	CG1972	CG1972	np	-4.8012	Downregulation of Notch pathway after Notch stimulation		no	N(delta)ecn Notch stimulation; m3-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00159-A	43506		CG1972	DRSC15407	np	none		no		Muscle assembly and maintenance	RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance.	Bai et al.	2008	18359903	Primary cells	primary embryonic cells	Muscle cell morphology	Fluorescence	np	Selected and random genes	dsRNA	Percentage of muscles with a given phenotype	Severe: > 80 %; medium: ~ 50 %
GR00183-A	43506	FBgn0039691	CG1972	33450	sp	GFP aggregates number, GFP aggregates size, ganglion mother cell shorter lineages, neuroblast underproliferation, lethal		no		Self-renewal and differentiation in neural stem cells	Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi.	Neumueller et al.	2011	21549331	Tissue	insc-GAL4	Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability	Fluorescence	np	Genome-wide	UAS-IR construct	Phenotype strength	> 1	Additional information about a secondary screen (KK library)
GR00189-A-1	43506	FBgn0039691	CG1972	BKN20820	np	Downregulation of Notch pathway		yes		Notch pathway regulation (1)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Cell line	S2	Notch pathway reporter	Luminescence	BKN	Genome-wide	dsRNA	Complex, sp	Complex criteria
GR00189-A-2	43506	FBgn0039691	CG1972	1972R-1	np	none		yes	C96-GAL4; final gene scoring: upregulation of Notch pathway	Notch pathway regulation (2)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4, C96-GAL4 and vg-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-2	43506	FBgn0039691	CG1972	1972R-2	strong	Lethal		yes	en-GAL4; final gene scoring: upregulation of Notch pathway	Notch pathway regulation (2)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4, C96-GAL4 and vg-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-2	43506	FBgn0039691	CG1972	1972R-1	sp	Upregulation of Notch pathway; wing disc size: overgrown; wing disc NRE:EGFP: up; wing disc myrRFP: accumulation; lethal		yes	en-GAL4	Notch pathway regulation (2)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4, C96-GAL4 and vg-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-2	43506	FBgn0039691	CG1972	1972R-2	sp	Upregulation of Notch pathway; wing pouch: spoon-like		yes	C96-GAL4	Notch pathway regulation (2)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	en-GAL4, C96-GAL4 and vg-GAL4	Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability	Fluorescence and visual inspection	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Phenotype strength	np
GR00189-A-3	43506	FBgn0039691	CG1972	np	np	none		no		Notch pathway regulation (3)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Tissue	GMR-GAL4	Notch pathway reporter	Fluorescence	Notch pathway component enriched RNAi library	Selected genes	UAS-IR construct	Average fluorescent intensity	Complex criteria
GR00190-A-1	43506	FBgn0039691	CG1972	33450	-0.392	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00218-S	43506	CG1972		AMB18277	0.39	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00220-A	43506	FBgn0039691	CG1972	BKN20820	-4.79	Decreased Srp/Lz-induced transcription		yes		Srp/Lz-induced transcriptional activation	A genome-wide RNA interference screen identifies a differential role of the mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila.	Gobert et al.	2010	20368357	Cell line	Kc167	Srp/Lz-induced transcription reporter (PO45)	Luminescence	BKN	Genome-wide	dsRNA	Z-score	> 4 OR < -4
GR00239-A-1	43506	CG1972		1972R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00245-A	43506	FBgn0039691	CG1972	DRSC15407	np	Increased formation of mutant Huntingtin (Httex1-Qn) protein aggregates		yes	validated	Huntingtin (Htt) aggregates formation	A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila.	Zhang et al.	2010	20100940	Cell line	S2	Mutant Httex1-Qn-eGFP protein expression and DNA content	Fluorescence	Version 1 and np	Genome-wide	dsRNA	Aggregate number, size, and signal intensity	> 2 standard deviations	Additional information about secondary screens (reproducibility and non-overlaping dsRNAs)
GR00282-A	43506	CG1972	CG1972	33451	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	43506	CG1972	CG1972	33450	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	43506	CG1972	IntS11	109408	-2.5094959381951987	Strongly increased transposon expression				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	43506	FBgn0039691	CG1972	DRSC15407	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	43506	CG1972	IntS11	109408	np	Effect on follicular epithelium morphology			further phenotype data in classification screen	Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00315-A-2	43506	CG1972	IntS11	109408	np	Defect in formation phase				Follicular epithelium development (2)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Selected genes	UAS-IR construct	Visual inspection	np	For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00326-A	43506	FBgn0039691	CG1972	1972	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
	43506	FBgn0039691	np	np	sp	none
GR00367-S	43506	CG1972		33450	np	liquid clearance defect			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	43506	CG1972		109408	-0.14161226570585694	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	43506	CG1972		GD33451		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.