Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 43587
Symbol: Mlc2
Description: Myosin light chain 2

DataSource: http://genomernai.dkfz.de/v16/genedetails/43587

Export (tab separated) Export to Excel

Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	43587	FBgn0002773	Mlc2	DRSC16741	1.699583695	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	43587	FBgn0002773	Mlc2	DRSC16741	0.093483066	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	43587			HFA16741	-0.4	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	43587			HFA16741	-0.6	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00045-A-0	43587	FBgn0002773		DRSC16741	-2.59	Decreased Hedgehog reporter activity		no		Hedgehog signaling	A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway.	Nybakken et al.	2005	16311596	Cell line	Clone 8	Hedgehog signaling	Dual luciferase	DRSC		dsRNA	Z-score	>
	43587	FBgn0002773	Mlc2	LD22691	sp	none		no	library: DGC1
	43587	FBgn0053196, FBgn0002773	dp, Mlc2	LP08904	sp	none		no	library: DGC2
GR00084-A-0	43587	FBgn0002773	CG2184	np	np	C. trachomatis infection down		no		C. trachomatis infection	RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry.	Elwell et al.	2008	18369471	Cell line	S2	C. trachomatis infection	High content (microscopy)	OpenBiosystems		dsRNA	Visual inspection	np
GR00159-A	43587	FBgn0002773	Mlc2	DRSC16741, DRSC32500, DRSC32501	severe	Muscles spread, actin and myosin filaments lack striation		no		Muscle assembly and maintenance	RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance.	Bai et al.	2008	18359903	Primary cells	primary embryonic cells	Muscle cell morphology	Fluorescence	np	Selected and random genes	dsRNA	Percentage of muscles with a given phenotype	Severe: > 80 %; medium: ~ 50 %
GR00175-A	43587	CG2184	Mlc2	DRSC32500	-0.0853169213505586	none		no		Hh pathway regulation	A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.	DasGupta et al.	2007	17903264	Cell line	rp	rp	Luminescence	DRSC-v	Selected genes	dsRNA	Average fractional change	Complex criteria
GR00175-A	43587	CG2184	Mlc2	DRSC32501	-0.189087908965102	none		no		Hh pathway regulation	A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.	DasGupta et al.	2007	17903264	Cell line	rp	rp	Luminescence	DRSC-v	Selected genes	dsRNA	Average fractional change	Complex criteria
GR00214-A-1	43587	FBgn0002773	Mlc2	DRSC28878	0.45	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	43587	FBgn0002773	Mlc2	DRSC28878	0.45	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	43587	FBgn0002773	Mlc2	DRSC28878	1.59	Increased ERK phosphorylation after sSpitz and EGF stimulation	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	43587	FBgn0002773	Mlc2	DRSC28878	0.09	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	43587	CG2184	Mlc2	AMB33179	-1.1	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00238-S-1	43587	CG2184	mlc2	104621	sp	Embryo lethal		yes	library: VDRC	Muscle development and maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	Mef2-GAL4	Climbing ability and viability	Negative geotaxis assay and visual inspection	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2	43587	CG2184	mlc2	104621	sp	none		no	library: VDRC	Muscle maintenance	The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.	Perkins et al.	2014	25977760	Tissue	TARGET Mef2-GAL4	Climbing ability	Negative geotaxis assay	np	Selected genes	UAS-IR construct	Complex, sp	Complex criteria	Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00282-A	43587	CG2184	Mlc2	51201	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	43587	CG2184	Mlc2	104621	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	43587	CG2184	Mlc2	104621	0.3278078746474332	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	43587	FBgn0002773	Mlc2	DRSC16741	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	43587	CG2184	Mlc2	104621	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00335-A	43587	FBgn0002773	Mlc2	JF01104	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	43587	FBgn0002773	Mlc2	HMS01583	np	F1 lethal				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	43587	FBgn0002773	Mlc2	JF01106	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	43587	FBgn0002773	np	np	sp	none
GR00367-S	43587	CG2184		104621	np	lethal or adult morphology phenotype			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	43587	CG2184		104621	-0.26382232012206785	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	43587	CG2184		GD51201		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.