Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 43693
Symbol: ATPsynC
Description: ATP synthase, subunit C

DataSource: http://genomernai.dkfz.de/v16/genedetails/43693

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	43693	FBgn0039830	CG1746	DRSC15236	-0.018285449	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	43693	FBgn0039830	CG1746	DRSC15236	13.05959162	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00014-A	43693		CG1746	DRSC15236	strong	Decreased Flag-Mad nuclear accumulation		no		Dpp pathway regulation	Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads.	Xu et al.	2007	17785517	Cell line	S2R+	Flag-Mad protein expression and subcellular location	Fluorescence	rp	Genome-wide	dsRNA	Visual inspection	np
GR00031-A-1	43693			HFA15236	0.9	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	43693			HFA15236	1.4	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	43693	FBgn0039830	CG1746	GM13193	sp	none		no	library: DGC1
GR00062-A	43693	FBgn0039830	CG1746	np	5	Decreased Ca2+ influx				Ca2+ channel regulation	CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.	Vig et al.	2006	16645049	Cell line	S2R+	Ca2+ influx	Fluorescence	np	Genome-wide	dsRNA	Complex, sp	>
GR00084-A-0	43693	FBgn0039830	CG1746	np	np	C. trachomatis infection down		no		C. trachomatis infection	RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry.	Elwell et al.	2008	18369471	Cell line	S2	C. trachomatis infection	High content (microscopy)	OpenBiosystems		dsRNA	Visual inspection	np
GR00128-A-1	43693	FBgn0039830	CG1746	DRSC15236	sp	none		no	hit according to not so stringent primary analysis	Combinatorial effect with methyl methanesulphonate (1)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Genome-wide	dsRNA	rp	rp	Additional information about the primary screen
GR00148-A	43693	FBgn0039830	CG1746	np	2.31	Increased P-JNK protein expression with PGN	15 min PGN induction	no		PGN-induced dJNK phosphorylation	A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling.	Bond and Foley	2009	19893628	Cell line	S2	P-JNK protein expression	Fluorescence	Custom-made	Genome-wide	dsRNA	Z-score	Complex criteria	Only hits stored in GenomeRNAi
GR00152-A	43693	CG1746	CG1746	np	-2.528	Downregulation of Notch pathway after Notch stimulation		no	N(delta)ecn Notch stimulation; con-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	43693	CG1746	CG1746	np	-0.1483	none		no	N(delta)ecn Notch stimulation; m3-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	43693	CG1746	CG1746	np	-1.4752	none		no	without Notch stimulation	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00159-A	43693		CG1746	DRSC15236	np	none		no		Muscle assembly and maintenance	RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance.	Bai et al.	2008	18359903	Primary cells	primary embryonic cells	Muscle cell morphology	Fluorescence	np	Selected and random genes	dsRNA	Percentage of muscles with a given phenotype	Severe: > 80 %; medium: ~ 50 %
GR00218-S	43693	CG1746		AMB27989	1.13	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	43693	CG1746		1746R	sp	Increased mannose Chp glycosylation		yes		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00239-A-2	43693	CG1746		1746R	np	none		no		Glycosylation regulation (2)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation	Luminescence	np	Selected genes	UAS-IR construct	p-value	< 0.005
GR00282-A	43693	CG1746	CG1746	106834	np	none			rudimentary ovaries	piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	43693	CG1746	CG1746	106834	-0.8464584581599697	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00305-A	43693	FBgn0039830	CG1746	np	2.3582206681642424	Increased Rift Valley fever virus (RVFV) infection			score in duplicate screen: 3.04	Rift Valley fever virus (RVFV) infection	A genome-wide RNAi screen reveals that mRNA decapping restricts bunyaviral replication by limiting the pools of Dcp2-accessible targets for cap-snatching	Hopkins et al.	2013	23824541	Cell line	DL1	RVFV N protein expression and DNA content	Fluorescence	Ambion	Genome-wide	dsRNA	Z-score	>	Additional secondary screen available. RVFV strain MP12 used in both screens.
GR00308-A	43693	CG1746	CG1746	np	2.33	Decreased cell aggregation				Cadherin-mediated cell-cell adhesion	A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cellâ€“cell adhesion	Toret et al.	2014	24446484	Cell line	S2	Cell aggregation	Fluorescence	V2 RNAi library (Thermo Fisher Scientific)	Genome-wide	dsRNA	Visual inspection; average of 3 replicates; 0	> 1.5	Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A	43693	FBgn0039830	CG1746	DRSC15236	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	43693	CG1746	CG1746	106834	np	Effect on follicular epithelium morphology			further phenotype data in classification screen	Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00315-A-2	43693	CG1746	CG1746	106834	np	No ovaries				Follicular epithelium development (2)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Selected genes	UAS-IR construct	Visual inspection	np	For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00326-A	43693	FBgn0039830	CG1746	1746	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00335-A	43693	FBgn0039830	CG1746	GL00390	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	43693	FBgn0039830	np	np	sp	none
GR00367-S	43693	CG1746		106834	np	lethal or adult morphology phenotype			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	43693	CG1746		106834	2.2599964552498655	Low decrease in nos and yTub expression			No eggs/larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2	43693	CG1746		106834	np	No germline, 100%				Defects in female germline	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	Vasa AND 1B1 expression	Immunofluorescence	VDRC	Selected genes	UAS-IR construct	np	np	Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	43693	CG1746		KK106834		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.