RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 43723
  • Symbol: RpL6
  • Description: Ribosomal protein L6
DataSource: http://genomernai.dkfz.de/v16/genedetails/43723

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 43723 FBgn0039857 RpL6 DRSC22384 0.719052009 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 43723 FBgn0039857 RpL6 DRSC14323 2.441083367 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 43723 FBgn0039857 RpL6 DRSC14323 3.343017511 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 43723 FBgn0039857 RpL6 DRSC22384 -2.692218569 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 43723 FBgn0039857 DRSC22384 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 43723 CG11522 HFA14323 3.2 Decreased cell number and viability yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 43723 CG11522 HFA14323 3.1 Decreased cell number and viability no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00039-A-0 43723 FBgn0039857 DRSC14323 np Drosophila C virus infection reduced no Drosophila C virus infection Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition. Cherry et al. 2005 15713840 Cell line S2 Drosophila C virus infection High content (microscopy) DRSC dsRNA Visual inspection > 40% reduced infection
GR00045-A-0 43723 FBgn0039857 DRSC14323 -3 Decreased Hedgehog reporter activity no Hedgehog signaling A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken et al. 2005 16311596 Cell line Clone 8 Hedgehog signaling Dual luciferase DRSC dsRNA Z-score >
GR00047-A-1 43723 FBgn0039857 RpL6 DRSC14323 sp Decreased horseradish peroxidase protein expression no Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
43723 FBgn0033807, FBgn0039857 AQP, RpL6 GH06377 sp none no library: DGC2
43723 FBgn0039857 RpL6 RE08669 sp Decreased viability yes library: DGC2
GR00048-A-2 43723 FBgn0039857 RpL6 np 1.087 none yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 43723 FBgn0039857 RpL6 np 468 none no Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00090-A-0 43723 FBgn0039857 CG11522 np strong (but ribosomal) More dispersed droplets no Lipid storage Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Guo et al. 2008 18408709 Cell line S2 Lipid droplet staining High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00128-A-1 43723 FBgn0039857 CG11522 DRSC14323 sp none no hit according to not so stringent primary analysis Combinatorial effect with methyl methanesulphonate (1) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Genome-wide dsRNA rp rp Additional information about the primary screen
GR00128-A-2 43723 FBgn0039857 CG11522 DRSC32254 sp Synthetic lethal with methyl methanesulphonate no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00131-A-1 43723 RpL6 DRSC25982 25 - 50 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00135-A-1 43723 CG11522 RpL6 51715 0 Developmentally lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 43723 CG11522 RpL6 51716 -0.6 Lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 43723 CG11522 51715 0.19 Larval lethal no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00152-A 43723 CG11522 RpL6 np 2.116 Upregulation of Notch pathway after Notch stimulation no N(delta)ecn Notch stimulation; m3-luc normalization Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00152-A 43723 CG11522 RpL6 np -1.4553 none no without Notch stimulation Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00152-A 43723 CG11522 RpL6 np -0.040967 none no N(delta)ecn Notch stimulation; con-luc normalization Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00170-A-1 43723 RpL6 DRSC14323 -3.2 none no decreased cell viability or Sanger collection sequence HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00175-A 43723 CG11522 RpL6 DRSC32254 -0.446201851491092 Downregulation of Hh pathway no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00175-A 43723 CG11522 RpL6 DRSC32255 -0.385313587544595 Downregulation of Hh pathway no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00189-A-1 43723 FBgn0039857 RpL6 BKN28144 np Downregulation of Notch pathway no Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00191-A-1 43723 CG11522 RpL6 np np Increased melanotic mass formation yes Blood cell homeostasis regulation (1) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-2 43723 CG11522 RpL6 np np none yes Blood cell homeostasis regulation (2) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-3 43723 CG11522 RpL6 np 4.8 none yes Blood cell homeostasis regulation (3) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-4 43723 CG11522 RpL6 np 6.7 Increased melanotic mass formation yes Blood cell homeostasis regulation (4) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-5 43723 CG11522 RpL6 np 0 none yes Blood cell homeostasis regulation (5) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-6 43723 CG11522 RpL6 np np Lethal yes confirmed melanotic suppressor gene, non-overlapping dsRNA Blood cell homeostasis regulation (6) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-7 43723 CG11522 RpL6 np 5 Increased melanotic mass formation yes confirmed melanotic suppressor gene, non-overlapping dsRNA Blood cell homeostasis regulation (7) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-8 43723 CG11522 RpL6 np np Lethal no confirmed melanotic suppressor gene, non-overlapping dsRNA Blood cell homeostasis regulation (8) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00214-A-1 43723 FBgn0039857 RpL6 DRSC14323 -3.46 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 43723 FBgn0039857 RpL6 DRSC14323 2.97 Increased ERK phosphorylation after EGF stimulation 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 43723 FBgn0039857 RpL6 DRSC14323 0.73 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 43723 FBgn0039857 RpL6 DRSC14323 4.07 Increased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 43723 CG11522 AMB26877 -2.92 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 43723 CG11522 11522R sp Decreased mannose Chp glycosylation yes Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00239-A-2 43723 CG11522 11522R np none no Glycosylation regulation (2) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation Luminescence np Selected genes UAS-IR construct p-value < 0.005
GR00273-A-1 43723 FBgn0039857 RpL6 DRSC22384 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00273-A-1 43723 FBgn0039857 RpL6 DRSC25982 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00273-A-1 43723 FBgn0039857 RpL6 DRSC14323 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00282-A 43723 CG11522 RpL6 51715 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 43723 CG11522 RpL6 51716 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 43723 CG11522 RpL6 107302 -0.6608445053358079 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00291-A 43723 RpL6 DRSC14323 sp Increased or decreased hedgehog (hh) secretion eliminated as hit by authors, sp Hedgehog (hh) secretion regulation A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila Aikin et al. 2012 22432040 Cell line S2 Hedgehog protein expression Luminescence DRSC Genome-wide dsRNA Z-score < -2 OR > 2 by at least 1/3 Z-scores 296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available.
GR00305-A 43723 FBgn0039857 RpL6 np 2.362851603205994 Increased Rift Valley fever virus (RVFV) infection score in duplicate screen: 4.72 Rift Valley fever virus (RVFV) infection A genome-wide RNAi screen reveals that mRNA decapping restricts bunyaviral replication by limiting the pools of Dcp2-accessible targets for cap-snatching Hopkins et al. 2013 23824541 Cell line DL1 RVFV N protein expression and DNA content Fluorescence Ambion Genome-wide dsRNA Z-score > Additional secondary screen available. RVFV strain MP12 used in both screens.
GR00308-A 43723 CG11522 RpL6 np 4 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 43723 FBgn0039857 RpL6 DRSC14323 np Actin defect, other cellular phenotype cell shape variable, decreased level of actin, decreased cell number, loss of cell monolayer Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 43723 CG11522 RpL6 107302 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
43723 FBgn0039857 np np sp none
GR00379-A-1 43723 CG11522 107302 3.7426890053566924 Medium decrease in nos and yTub37c expression No eggs/larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2 43723 CG11522 107302 np No germline, 100% Defects in female germline Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 Vasa AND 1B1 expression Immunofluorescence VDRC Selected genes UAS-IR construct np np Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 43723 CG11522 BL34004 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 43723 CG11522 GD51716 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.