RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 43839
  • Symbol: eIF4G1
  • Description: eukaryotic translation initiation factor 4G1
DataSource: http://genomernai.dkfz.de/v16/genedetails/43839

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 43839 FBgn0023213 eIF-4G DRSC17218 6.400363835 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 43839 FBgn0023213 eIF-4G DRSC17218 5.320682683 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 43839 FBgn0023213 DRSC17218 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 43839 HFA17218 1.9 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 43839 HFA17218 0 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
43839 FBgn0023213 eIF-4G GM10051 sp Increased G1 DNA content, decreased G2 DNA content, decreased G1 cell size yes library: DGC1
43839 FBgn0023213 eIF-4G RE51676 sp none yes library: DGC2
GR00048-A-2 43839 FBgn0023213 eIF-4G np 1.236 none yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 43839 FBgn0023213 eIF-4G np 431 none no Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00092-A 43839 FBgn0023213 CG10811 RDM1190-7301638 np Abnormal spindle, monopolar spindle, increased number of cells in prometaphase no Microtubule assembly and organization Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. Ducat et al. 2008 18463163 Cell line S2 DNA content, alpha-tubulin and CP309 protein expression Fluorescence OpenBiosystems Drosophila RNAi Library 1.0 Mitotic regulator genes dsRNA Complex, sp Complex criteria
GR00130-A 43839 FBgn0023213 eIF-4G np <= -2 Decreased mutant human huntingtin aggregation no Mutant human huntingtin aggregation RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. Doumanis et al. 2009 19789644 Cell line BG2-c2 Nhtt(62Q)EGFP aggregate number and size Fluorescence OpenBiosystems RNAi library Selected genes dsRNA Z-score Suppressor: <
GR00134-A-1 43839 CG10811 eIF4G 17002 np Lethal yes early larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 43839 CG10811 eIF4G 17003 np Lethal yes early larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-3 43839 CG10811 eIF4G 17002, 17003 np Split myofibril, fading Z-line no Muscle morphogenesis and function (3) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 ZCL0663 protein expression Fluorescence np Selected genes UAS-IR construct np np
GR00135-A-1 43839 CG10811 eIF4G 17002 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 43839 CG10811 eIF4G 17003 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 43839 CG10811 17003 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 43839 CG10811 eIF4G 17003 -0.655913978494623 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00142-A-1 43839 CG10811 eIF4G 17003 -0.487179487179487 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 43839 CG10811 17002 sp Completely lethal (before pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 43839 CG10811 17003 sp Completely lethal (before pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00159-A 43839 eIF-4G DRSC17218 np none no Muscle assembly and maintenance RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Bai et al. 2008 18359903 Primary cells primary embryonic cells Muscle cell morphology Fluorescence np Selected and random genes dsRNA Percentage of muscles with a given phenotype Severe: > 80 %; medium: ~ 50 %
GR00170-A-1 43839 eIF_4G DRSC17218 -6.9 none no decreased cell viability or Sanger collection sequence HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00172-A-1 43839 CG10811, CG6754 eIF4G, nbs DRSC17218, custom (nbs) np Increased histone H3 phosphorylation with doxorubicin yes G2-M DNA damage checkpoint regulation (1) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Genome-wide dsRNA Mitotic cell number per well > 20
GR00172-A-1 43839 CG10811, CG6754 eIF4G, nbs DRSC29786, custom (nbs) np Increased histone H3 phosphorylation with doxorubicin yes G2-M DNA damage checkpoint regulation (1) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Genome-wide dsRNA Mitotic cell number per well > 20
GR00172-A-2 43839 CG10811 eIF4G DRSC17218, DRSC29786 31.11 Increased histone H3 phosphorylation with bleocin yes with bleocin G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 43839 CG10811 eIF4G DRSC17218, DRSC29786 31.4 Increased histone H3 phosphorylation with doxorubicin yes with doxorubicin G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 43839 CG10811 eIF4G DRSC17218, DRSC29786 49.62 Increased histone H3 phosphorylation with etoposide yes with etoposide G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2 43839 CG10811 eIF4G DRSC17218, DRSC29786 69.74 Increased histone H3 phosphorylation after X-ray exposure yes X-ray exposure G2-M DNA damage checkpoint regulation (2) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Selected genes dsRNA Relative mitotic index Complex criteria Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00190-A-1 43839 FBgn0023213 eIF4G 17003 0.103 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00190-A-1 43839 FBgn0023213 eIF4G 17003 -0.042 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00214-A-1 43839 FBgn0023213 eIF4G DRSC17218 -1.4 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 43839 FBgn0023213 eIF4G DRSC17218 -2.41 Decreased ERK phosphorylation after EGF stimulation 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 43839 FBgn0023213 eIF4G DRSC17218 -1.07 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 43839 FBgn0023213 eIF4G DRSC17218 -0.53 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 43839 CG10811 eIF4G AMB33482 0.35 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00282-A 43839 CG10811 eIF4G 17003 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 43839 CG10811 eIF4G 17002 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 43839 CG10811 eIF4G 17002 0.7596779742614859 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00291-A 43839 eIF-4G DRSC17218 sp Increased or decreased hedgehog (hh) secretion eliminated as hit by authors, sp Hedgehog (hh) secretion regulation A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila Aikin et al. 2012 22432040 Cell line S2 Hedgehog protein expression Luminescence DRSC Genome-wide dsRNA Z-score < -2 OR > 2 by at least 1/3 Z-scores 296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available.
GR00308-A 43839 CG10811 eIF-4G np 2.67 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 43839 FBgn0023213 eIF4G DRSC17218 np Actin defect, microtubule defect, other cellular phenotype decreased cell size, decreased level of actin, microtubule clumps Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00314-A 43839 FBgn0023213 eIF4G RNA005206 66.57 Decreased MAPK phosphorylation RAS-related MAPK activation A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila Ashton-Beaucage et al. 2014 24643257 Cell line S2 MAPK phosphorylation Fluorescence Custom-made Genome-wide dsRNA pMAPK signal (%GFP dsRNA) np S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai
GR00335-A 43839 FBgn0023213 eIF4G HMS00762 np F1 lethal F1 larval lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
43839 FBgn0023213 np np sp none
GR00367-S 43839 CG10811 17002 np liquid clearance defect VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 43839 CG10811 17002 -0.5134406205590275 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 43839 CG10811 GD17002 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 43839 CG10811 BL33049 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 43839 CG10811 GD17003 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.