Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 44259
Symbol: nbs
Description: nbs

DataSource: http://genomernai.dkfz.de/v16/genedetails/44259

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	44259	FBgn0026198	nbs	DRSC11373	-0.051988402	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	44259	FBgn0026198	nbs	DRSC11373	0.037498769	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0	44259	FBgn0086349		DRSC11373	weak	ERK activation increased		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	44259			HFA11373	1	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	44259			HFA11373	-0.2	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	44259	FBgn0026198	nbs	LD44438	sp	none		no	library: DGC1
GR00128-A-2	44259	FBgn0086349	CG6754	DRSC23793	sp	none		no		Combinatorial effect with methyl methanesulphonate (2)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Selected genes	dsRNA	rp	rp
GR00134-A-1	44259	CG6754	nbs	28215	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	44259	CG6754	nbs	28216	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	44259	CG6754	nbs	28215	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies Î±2Î´3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1	44259	CG6754	nbs	28216	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies Î±2Î´3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	44259	CG6754		28216	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCÎ”4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00142-A-1	44259	CG6754	CG6754	28216	0.231343283582089	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	44259	CG6754		28215	1	Loss of bristles		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	44259	CG6754		28216	sp	Colour defects, notum malformation death		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00172-A-1	44259	CG9797, CG6754	CG9797, nbs	custom (CG9797), custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		yes		G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00172-A-1	44259	CG3018, CG6754	lwr, nbs	custom (lwr), custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		yes		G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00172-A-1	44259	CG7413, CG6754	Rbf, nbs	custom (Rbf), custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		yes		G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00172-A-1	44259	CG9633, CG6754	RpA-70, nbs	custom (RpA-70), custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		yes		G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00172-A-1	44259	CG8068, CG6754	Su(var)2-10, nbs	custom (Su(var)2-10), custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		yes		G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00172-A-1	44259	CG15104, CG6754	Topors, nbs	custom (Topors), custom (nbs)	np	Increased histone H3 phosphorylation with doxorubicin		yes		G2-M DNA damage checkpoint regulation (1)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Genome-wide	dsRNA	Mitotic cell number per well	> 20
GR00172-A-2	44259	CG6754	nbs	custom (nbs)	9.31	none		yes	with bleocin	G2-M DNA damage checkpoint regulation (2)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Selected genes	dsRNA	Relative mitotic index	Complex criteria	Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2	44259	CG6754	nbs	custom (nbs)	27.41	Increased histone H3 phosphorylation with doxorubicin		yes	with doxorubicin	G2-M DNA damage checkpoint regulation (2)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Selected genes	dsRNA	Relative mitotic index	Complex criteria	Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2	44259	CG6754	nbs	custom (nbs)	27.44	Increased histone H3 phosphorylation with etoposide		yes	with etoposide	G2-M DNA damage checkpoint regulation (2)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Selected genes	dsRNA	Relative mitotic index	Complex criteria	Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00172-A-2	44259	CG6754	nbs	custom (nbs)	24.95	none		yes	X-ray exposure	G2-M DNA damage checkpoint regulation (2)	A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint.	Kondo and Perrimon	2011	21205937	Cell line	S2R+	Histone H3 phosphorylation	Fluorescence	DRSC 2.0 and custom-made	Selected genes	dsRNA	Relative mitotic index	Complex criteria	Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field.
GR00190-A-1	44259	FBgn0086349	nbs	28216	-0.319	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00214-A-1	44259	FBgn0261530	nbs	DRSC11373	0.69	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	44259	FBgn0261530	nbs	DRSC11373	0.1	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	44259	FBgn0261530	nbs	DRSC11373	0.95	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	44259	FBgn0261530	nbs	DRSC11373	0.09	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	44259	CG6754	nbs	AMB19675	1.13	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	44259	CG6754	nbs	28216	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	44259	CG6754	nbs	28215	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	44259	CG6754	nbs	110366	-0.500534331725396	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	44259	FBgn0086349	nbs	DRSC11373	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	44259	CG6754	nbs	110366	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
	44259	FBgn0261530	np	np	sp	none
GR00367-S	44259	CG6754		28216	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	44259	CG6754		110366	-0.44826152797702246	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A	44259			110366	0.3046444647607438	none				Regulation of olfactory memory formation	Identification of genes that promote or inhibit olfactory memory formation in Drosophila.	Walkinshaw et al.	2015	25644700	Organism	Nsyb-GAL4	T-maze performance	Visual inspection	VDRC	Selected genes	UAS-IR construct	Performance index, sp	High performer in olfactory memory formation: >	Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A	44259	CG6754		GD28216		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.