RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 45826
  • Symbol: bel
  • Description: belle
DataSource: http://genomernai.dkfz.de/v16/genedetails/45826

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 45826 FBgn0000171 bel DRSC16555 -1.318979737 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 45826 FBgn0000171 bel DRSC16555 -0.04997263 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00004-A-0 45826 bel DRSC16555 1.27 siRNA reporter upregulated no Drosophila small RNA pathways Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Zhou et al. 2008 19026789 Cell line S2 miRNA and siRNA pathway activity Dual luciferase DRSC dsRNA Fold change endo-siRNA reporter 1.5/0.6
GR00021-A-0 45826 FBgn0000171 DRSC16555 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 45826 HFA16555 1.2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 45826 HFA16555 1.7 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00035-A 45826 CG9748 bel KP037 sp none no Alternative splicing Identification of alternative splicing regulators by RNA interference in Drosophila. Park et al. 2004 15492211 Cell line S2 dAdar, para and Dscam mRNA expression Reverse transcription PCR and autoradiography Custom-made RNA-binding protein genes and spliceosomal genes dsRNA np Alternative exon splicing for >
GR00040-A-0 45826 FBgn0000171 DRSC16555 2.84 Increased Wnt reporter activity no Wnt signaling Functional genomic analysis of the Wnt-wingless signaling pathway. DasGupta et al. 2005 15817814 Cell line Clone 8 Wnt signaling Dual luciferase DRSC dsRNA Z-score >
GR00045-A-0 45826 FBgn0000171 DRSC16555 6.5 Increased Hedgehog reporter activity no Hedgehog signaling A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken et al. 2005 16311596 Cell line Clone 8 Hedgehog signaling Dual luciferase DRSC dsRNA Z-score >
45826 FBgn0000171 bel RE28061 sp Decreased G1 DNA content yes library: DGC2
GR00048-A-2 45826 FBgn0000171 bel np 1.231 none yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 45826 FBgn0000171 bel np 455 none yes Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-4 45826 FBgn0000171 bel np 0.6 Increased number of mitotic cells no Cell size and cell-cycle regulation (4) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Number of mitotic cells Fluorescence Selected genes dsRNA Percentage Visual inspection Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00052-A 45826 bel DRSC16555 np Decreased Ca2+ uptake after thapsigargin stimulation no Store-operated calcium influx regulation Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity. Zhang et al. 2006 16751269 Cell line S2 Ca2+ uptake Fluorescence np Genome-wide dsRNA Z-score < -3
GR00068-A-0 45826 FBgn0000171 belle np np Decreased RNAi effect no RNAi pathway Double-stranded RNA is internalized by scavenger receptor-mediated endocytosis in Drosophila S2 cells. Ulvila et al. 2006 16531407 Cell line S2 RNAi activity Viability and FACS Custom-made library dsRNA np np
GR00131-A-1 45826 bel DRSC16555 25 - 50 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00134-A-1 45826 CG9748 bel 6299 np Lethal no late larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 45826 CG9748 bel 6299 0 Developmentally lethal no Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00142-A-1 45826 CG9748 bel 6299 -0.2 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 45826 CG9748 6299 sp Completely lethal (before pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00148-A 45826 FBgn0000171 bel np -2.78 Decreased P-JNK protein expression with PGN 15 min PGN induction no PGN-induced dJNK phosphorylation A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. Bond and Foley 2009 19893628 Cell line S2 P-JNK protein expression Fluorescence Custom-made Genome-wide dsRNA Z-score Complex criteria Only hits stored in GenomeRNAi
GR00153-A 45826 bel np 3.2 Upregulation of Wnt/beta-catenin pathway without ∆NLrp6 no Wnt/beta-catenin pathway regulation Bili inhibits Wnt/beta-catenin signaling by regulating the recruitment of axin to LRP6. Kategaya et al. 2009 19572019 Cell line clone 8 beta-catenin dTF12 reporter Luminescence rp Genome-wide dsRNA Fold change > 2
GR00153-A 45826 bel np 3 Upregulation of Wnt/beta-catenin pathway after ∆NLrp6 stimulation with ∆NLrp6 no Wnt/beta-catenin pathway regulation Bili inhibits Wnt/beta-catenin signaling by regulating the recruitment of axin to LRP6. Kategaya et al. 2009 19572019 Cell line clone 8 beta-catenin dTF12 reporter Luminescence rp Genome-wide dsRNA Fold change > 2
GR00175-A 45826 CG9748 bel DRSC31930 0.224773960650947 none no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00175-A 45826 CG9748 bel DRSC31929 0.368531833863614 none no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00189-A-1 45826 FBgn0000171 bel BKN28143 np Downregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 45826 FBgn0000171 bel 6299 sp Upregulation of Notch pathway; wing pouch: spoon-like yes C96-GAL4 Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 45826 FBgn0000171 bel 6299 very strong Lethal yes en-GAL4; final gene scoring: upregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 45826 FBgn0000171 bel np np none no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00190-A-1 45826 FBgn0000171 bel 6299 -0.405 Decreased triglyceride expression yes Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00190-A-2 45826 FBgn0000171 bel 6299 d/l Lethal or developmental delay no Adiposity regulation (2) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue nsyb-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-3 45826 FBgn0000171 bel 6299 -0.536 Decreased triglyceride expression no Adiposity regulation (3) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue C57-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-4 45826 FBgn0000171 bel 6299 d/l Lethal or developmental delay no Adiposity regulation (4) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue oe-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-5 45826 FBgn0000171 bel 6299 d/l Lethal or developmental delay no Adiposity regulation (5) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue ppl-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00214-A-1 45826 FBgn0000171 bel DRSC25066 -0.81 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 45826 FBgn0000171 bel DRSC25066 0.19 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 45826 FBgn0000171 bel DRSC25066 3.25 Increased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 45826 FBgn0000171 bel DRSC25066 0.36 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 45826 CG9748 bel AMB24232 2.29 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00220-A 45826 FBgn0000171 bel BKN28143 -4.24 Decreased Srp/Lz-induced transcription yes Srp/Lz-induced transcriptional activation A genome-wide RNA interference screen identifies a differential role of the mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila. Gobert et al. 2010 20368357 Cell line Kc167 Srp/Lz-induced transcription reporter (PO45) Luminescence BKN Genome-wide dsRNA Z-score > 4 OR < -4
GR00239-A-1 45826 CG9748 9748Rb np Lethal no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 45826 CG9748 bel 6299 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 45826 CG9748 bel 6299 -0.045649635008374406 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 45826 FBgn0000171 bel DRSC16555 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A 45826 FBgn0263231 bel JF02884 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
45826 np np HFA16555 -0.39 none fold change: 1.17
45826 FBgn0000171 np np sp none
45826 FBgn0263231 np np 1.44 none fold change: 0.54
GR00367-S 45826 CG9748 6299 np liquid clearance defect VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 45826 CG9748 6299 0.5511151619575877 none Reduced eggs/larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2 45826 CG9748 6299 np none Defects in female germline Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 Vasa AND 1B1 expression Immunofluorescence VDRC Selected genes UAS-IR construct np np Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 45826 CG9748 BL28049 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 45826 CG9748 GD6299 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.