| GR00002-A |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
-2.247452592 |
Decreased lipid droplet area |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
-0.699801128 |
Decreased lipid droplet area |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
-0.182863104 |
Decreased lipid droplet area |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
-1.749361709 |
Decreased lipid droplet area |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00026-A-0 |
45928 |
FBgn0003392 |
shibire |
np |
np |
Morphology |
|
no |
|
Cell morphology |
A functional genomic analysis of cell morphology using RNA interference. |
Kiger et al. |
2003 |
14527345 |
Cell line |
Kc167, S2R+ |
Morphology |
High content (microscopy) |
Custom-made |
|
dsRNA |
Visual inspection |
np |
|
GR00031-A-1 |
45928 |
|
|
HFA20373 |
-0.7 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
45928 |
|
|
HFA20373 |
0.2 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
45928 |
FBgn0003392 |
shi |
DRSC20373 |
medium |
Binucleate cells |
|
no |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
45928 |
FBgn0003392 |
shi |
LD21622 |
sp |
none |
|
no |
library: DGC1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00076-A |
45928 |
CG18102 |
Dynamin |
np |
2.59 |
none |
|
no |
|
CD4 regulation by human immunodeficiency virus Type 1 Nef (HIV-1 Nef) |
Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor. |
Chaudhuri et al. |
2007 |
17267500 |
Cell line |
S2 |
Human CD4 surface protein expression |
Fluorescence |
Expression Arrest Drosophila RNAi library |
Protein trafficking machinery genes |
dsRNA |
Fold downregulation |
np |
|
|
45928 |
FBgn0003392 |
shi |
np |
0.585 |
Decreased mitochondrial citrate synthase activity |
|
yes |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00134-A-1 |
45928 |
CG18102 |
shi |
3799 |
np |
Lethal |
|
yes |
early larval |
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00134-A-1 |
45928 |
CG18102 |
shi |
3798 |
np |
Lethal |
|
yes |
early pupal |
Muscle morphogenesis and function (1) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00134-A-2 |
45928 |
CG18102 |
shi |
np |
np |
Wing posture / locomotion / ability to fly defect or lethal / semi-lethal |
|
no |
|
Muscle morphogenesis and function (2) |
Systematic genetic analysis of muscle morphogenesis and function in Drosophila. |
Schnorrer et al. |
2010 |
20220848 |
Tissue |
Mef2-GAL4 |
Posture, locomotion, flight and viability |
Visual inspection |
Custom-made |
Selected genes |
UAS-IR construct |
rp |
S19 > 0.5 |
|
GR00135-A-1 |
45928 |
CG18102 |
shi |
3799 |
0.46 |
none |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00139-A |
45928 |
|
shi |
3798 |
sp |
Lethal |
|
no |
|
Fly viability and adult morphology |
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. |
Dietzl et al. |
2007 |
17625558 |
Organism |
Act5C-GAL4 |
Adult morphology and viability |
Visual inspection |
Custom-made |
Selected and random genes |
UAS-IR construct |
Phenotype strength |
np |
Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) |
GR00142-A-1 |
45928 |
CG18102 |
shi |
3798 |
-0.428571428571428 |
none |
|
no |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00144-A-4 |
45928 |
CG18102 |
|
3798 |
sp |
Completely lethal (pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-4 |
45928 |
CG18102 |
|
3799 |
sp |
Completely lethal (before pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00189-A-3 |
45928 |
FBgn0003392 |
shi |
np |
np |
Upregulation of Notch pathway |
|
yes |
|
Notch pathway regulation (3) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
GMR-GAL4 |
Notch pathway reporter |
Fluorescence |
Notch pathway component enriched RNAi library |
Selected genes |
UAS-IR construct |
Average fluorescent intensity |
Complex criteria |
|
GR00189-A-4 |
45928 |
FBgn0003392 |
shi |
3799 |
sp |
Wing disc NRE:EGFP: up, broader, gap; wing disc myrRFP: accumulation |
|
no |
en-GAL4; final gene scoring: lethal and/or indeterminable effect on Notch pathway |
Notch pathway regulation (4) |
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. |
Saj et al. |
2010 |
20493818 |
Tissue |
en-GAL4 and C96-GAL4 |
Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability |
Fluorescence and visual inspection |
np |
Selected genes |
UAS-IR construct |
Phenotype strength |
np |
|
GR00190-A-1 |
45928 |
FBgn0003392 |
shi |
3798 |
0.36 |
none |
|
no |
|
Adiposity regulation (1) |
Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. |
Pospisilik et al. |
2010 |
20074523 |
Organism |
Hsp70-GAL4;Tub-GAL80ts |
Total fly triglyceride expression |
Colorimetrics |
np |
Genome-wide |
UAS-IR construct |
Triglyceride change |
Z-score > 1.65 after 3 screening rounds |
Additional information about the primary screen |
GR00214-A-1 |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
1.65 |
Increased ERK phosphorylation after EGF stimulation |
0 minutes EGF stimulation |
yes |
|
RTK-Ras-ERK pathway regulation (1) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
S2R+ |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00214-A-1 |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
-0.95 |
none |
10 minutes EGF stimulation |
yes |
|
RTK-Ras-ERK pathway regulation (1) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
S2R+ |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00214-A-2 |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
-1.25 |
none |
10 minutes sSpitz stimulation |
no |
|
RTK-Ras-ERK pathway regulation (2) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
Kc167 |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00214-A-2 |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
-1.58 |
Decreased ERK phosphorylation after sSpitz and EGF stimulation |
30 minutes sSpitz stimulation |
no |
|
RTK-Ras-ERK pathway regulation (2) |
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. |
Friedman et al. |
2011 |
22028469 |
Cell line |
Kc167 |
ERK phosphorylation |
Fluorescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 1.5 OR < -1.5 |
|
GR00218-S |
45928 |
CG18102 |
shi |
AMB19769 |
0.44 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
45928 |
CG18102 |
|
18102R |
np |
Decreased Chp protein expression |
|
no |
not detectable Chp level |
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00282-A |
45928 |
CG18102 |
shi |
3798 |
np |
none |
|
|
|
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00282-A |
45928 |
CG18102 |
shi |
3799 |
np |
none |
|
|
|
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
45928 |
CG18102 |
shi |
105971 |
0.12151256069417346 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00296-A |
45928 |
|
shi |
np |
-15.25 |
Decreased Sindbis virus (SINV) infection |
|
|
prim. screen rep. B: -18.44; sec. val. screen: -2.57 |
Sindbis virus (SINV) infection |
Genome-wide RNAi Screen Identifies SEC61A and VCP as Conserved Regulators of Sindbis Virus Entry |
Panda et al. |
2013 |
24332855 |
Cell line |
DL1 |
Sindbis virus (SINV) GFP protein expression |
Fluorescence |
Ambion |
Genome-wide |
dsRNA |
Z-score |
< -2 OR > 2 |
Primary screen performed in duplicate. Z-scores from replicate B and from secondary validation screen are shown in the comment field. Recombinant SINV (HRsp) expressing GFP was used for infection. |
GR00311-A |
45928 |
FBgn0003392 |
shi |
DRSC20373 |
np |
Actin defect, microtubule defect, other cellular phenotype |
|
|
cell shape variable, decreased peripheral actin, microtubule processes, loss of cell monolayer |
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00315-A-1 |
45928 |
CG18102 |
shi |
105971 |
np |
Effect on follicular epithelium morphology |
|
|
further phenotype data in classification screen |
Follicular epithelium development (1) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Random genes |
UAS-IR construct |
Visual inspection |
np |
Further classification screen available, see follicular epithelium development (2). |
GR00315-A-2 |
45928 |
CG18102 |
shi |
105971 |
np |
Defect in formation and maturation phase |
|
|
|
Follicular epithelium development (2) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. |
GR00335-A |
45928 |
FBgn0003392 |
shi |
JF03133 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
GR00335-A |
45928 |
FBgn0003392 |
shi |
HMS00154 |
np |
F1 lethal |
|
|
F1 embryonic lethal |
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
|
45928 |
FBgn0003392 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
45928 |
CG18102 |
|
3799 |
np |
liquid clearance defect |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00379-A-1 |
45928 |
CG18102 |
|
105971 |
-0.17002648693745728 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00388-A |
45928 |
CG18102 |
|
KK105971 |
|
none |
|
|
excess of ISC or EB |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
45928 |
CG18102 |
|
GD3798 |
|
none |
|
|
excess of ISC or EB |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
45928 |
CG18102 |
|
BL28513 |
|
Lethal |
|
|
excess of ISC or EB |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
45928 |
CG18102 |
|
GD3799 |
|
none |
|
|
excess of ISC or EB |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |