RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 48632
  • Symbol: Act87E
  • Description: Actin 87E
DataSource: http://genomernai.dkfz.de/v16/genedetails/48632

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 48632 HDC13406 DRSC14104 -1.976196565 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 48632 HDC13406 DRSC14104 -0.412419262 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 48632 FBgn0000046 Act87E DRSC22578 0.803343363 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 48632 FBgn0000046 Act87E DRSC22578 1.829126629 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00013-A 48632 CG18290 Act87E np np Decreased Chlamydia caviae infection no Chlamydia caviae infection RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection. Derre et al. 2007 17967059 Cell line SL2 Chlamydia MOMP and LPS expression and DNA content Fluorescence np Genome-wide dsRNA Visual inspection np Additional information about secondary screens
GR00031-A-1 48632 HFA14104 0.8 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 48632 HFA14104 0.8 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00032-A-0 48632 FBgn0003041 67096 np Multi-nucleate cells no Cytokinesis Terminal cytokinesis events uncovered after an RNAi screen. Echard et al. 2004 15380073 Cell line S2 Multinucleate cell incidence High content (microscopy) OpenBiosystems dsRNA Visual inspection np
48632 FBgn0000046 Act87E DRSC14104 weak Binucleate cells with microtubule extensions no
GR00034-A 48632 Actin np 12 Increased Dipt::lacZ protein expression without lipopolysaccharide stimulation, CDRi Imd pathway regulation Functional dissection of an innate immune response by a genome-wide RNAi screen. Foley and O'Farrell 2004 15221030 Cell line S2 Dipt::lacZ protein expression Colorimetrics Custom-made Selected genes dsRNA Percentage LacZ-positive cells Complex criteria Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish)
GR00034-A 48632 Actin np 22 Increased Dipt::lacZ protein expression after lipopolysaccharide stimulation with lipopolysaccharide stimulation, EDRi Imd pathway regulation Functional dissection of an innate immune response by a genome-wide RNAi screen. Foley and O'Farrell 2004 15221030 Cell line S2 Dipt::lacZ protein expression Colorimetrics Custom-made Selected genes dsRNA Percentage LacZ-positive cells Complex criteria Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish)
48632 FBgn0000046 Act87E RE12057 sp none no library: DGC2
48632 FBgn0000046 Act87E RE14441 sp none no library: DGC2
GR00159-A 48632 FBgn0000046 Act87E DRSC22578, DRSC23961 np Muscles with short and/or thin myofibrils no Muscle assembly and maintenance RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Bai et al. 2008 18359903 Primary cells primary embryonic cells Muscle cell morphology Fluorescence np Selected and random genes dsRNA Percentage of muscles with a given phenotype Severe: > 80 %; medium: ~ 50 %
GR00172-A-1 48632 Act87E, Act5C, Act88F, Act57B, Act79B, nbs DRSC14104, custom (nbs) np Increased histone H3 phosphorylation with doxorubicin no excluded from further analysis: role in cytokinesis G2-M DNA damage checkpoint regulation (1) A genome-wide RNAi screen identifies core components of the Gâ‚‚-M DNA damage checkpoint. Kondo and Perrimon 2011 21205937 Cell line S2R+ Histone H3 phosphorylation Fluorescence DRSC 2.0 and custom-made Genome-wide dsRNA Mitotic cell number per well > 20
GR00214-A-1 48632 DRSC14104 0.48 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 48632 DRSC14104 -0.56 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 48632 DRSC14104 -0.15 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 48632 DRSC14104 1.87 Increased ERK phosphorylation after sSpitz and EGF stimulation 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00216-A 48632 FBgn0000046 Act87E BKN31781 -0.1 none no Wnt proteins secretion p24 proteins are required for secretion of Wnt ligands. Buechling et al. 2011 22094269 Cell line S2R+ Wg pathway reporter Luminescence BKN Cellular transporting machinery genes dsRNA Z-score > 2.5 OR < -2.5 Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S 48632 CG18290 Act87E AMB31425 1.27 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00238-S-1 48632 CG18290 act87E 102480 sp Pupal lethal yes library: VDRC Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2 48632 CG18290 act87E 102480 sp Climbing defect no library: VDRC Muscle maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue TARGET Mef2-GAL4 Climbing ability Negative geotaxis assay np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1 48632 CG18290 18290R np Lethal no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 48632 CG18290 Act87E 102480 np none not expressed piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 48632 CG18290 Act87E 102480 -0.35875161969012187 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00308-A 48632 CG18290 np 1.67 Decreased cell aggregation validated in secondary screen Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 48632 FBgn0000046 Act87E DRSC14104 np Actin defect, microtubule defect, other cellular phenotype decreased cell size, cell shape round or non-adherent, decreased level of actin, microtubule processes, increased number of multinucleate cells, loss of cell monolayer Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00326-A 48632 FBgn0000046 Act87E 18290 np none Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
48632 FBgn0000046 np np sp none
48632 np np DRSC14104 -1.47 none fold change: 1.82
GR00367-S 48632 CG18290 102480 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 48632 CG18290 102480 -0.1791238437725674 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 48632 CG18290 KK102480 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.